Restriction map for
pFN21AB6668
(Circular) (Six-base) MAPSORT of: pFN21AB6668.seq Check: 6942 from: 1 to: 6064
pFN21AB6668 6064 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2319 3338 3407 1937
Size: 382 1019 69 4594
Fragments arranged by size:
4594 1019 382 69
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 2182 4191 4564 1563
Size: 619 2009 373 3063
Fragments arranged by size:
3063 2009 619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 2996 3096 4127 5511 5779 1632
Size: 575 789 100 1031 1384 268 1917
Fragments arranged by size:
1917 1384 1031 789 575 268 100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 2059 4963 5940 1203
Size: 856 2904 977 1327
Fragments arranged by size:
2904 1327 977 856
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 2738 4793 2738
Size: 2055 4009
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 2105 2704 3277 3807 5379 2105
Size: 599 573 530 1572 2790
Fragments arranged by size:
2790 1572 599 573 530
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 3373 3599 3751 5860 1493
Size: 1880 226 152 2109 1697
Fragments arranged by size:
2109 1880 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 3389 1870
Size: 73 1446 4545
Fragments arranged by size:
4545 1446 73
BamHI G'GATC_C
Cuts at: 3394 3761 3394
Size: 367 5697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2482 3385 4840 618
Size: 325 217 627 695 903 1455 1842
Fragments arranged by size:
1842 1455 903 695 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3383 1391
Size: 1992 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2206 2726 3295 928
Size: 971 307 520 569 3697
Fragments arranged by size:
3697 971 569 520 307
BbvCI CC'TCA_GC
Cuts at: 2148 3294 2148
Size: 1146 4918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2009 2043 4255 4289 1903
Size: 34 72 34 2212 34 3678
Fragments arranged by size:
3678 2212 72 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BfrBI ATG'CAT
Cuts at: 2282 2282
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2893 4675 136
Size: 107 122 71 631 605 1221 1782 1525
Fragments arranged by size:
1782 1525 1221 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2490 2809 3321 6059 2490
Size: 319 512 2738 2495
Fragments arranged by size:
2738 2495 512 319
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 2485 3263 4125 5281 1790
Size: 695 778 862 1156 2573
Fragments arranged by size:
2573 1156 862 778 695
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3795 4753 442
Size: 416 53 83 399 24 2378 958 1753
Fragments arranged by size:
2378 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3053 4724 1239
Size: 1814 1671 2579
Fragments arranged by size:
2579 1814 1671
Bpu10I CC'TnA_GC
Cuts at: 2148 3294 2148
Size: 1146 4918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2977 4189 5054 5352 2977
Size: 1212 865 298 3689
Fragments arranged by size:
3689 1212 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3046 4727 882
Size: 617 1547 1681 2219
Fragments arranged by size:
2219 1681 1547 617
BsaAI yAC'GTr
Cuts at: 493 1204 3855 493
Size: 711 2651 2702
Fragments arranged by size:
2702 2651 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2739 3233 3871 4253
Size: 53 83 186 936 1206 494 638 382
Cuts at: 4253 4888 275
Size: 635 1451
Fragments arranged by size:
1451 1206 936 638 635 494 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4497 4895 5169 5316 5727 4497
Size: 398 274 147 411 4834
Fragments arranged by size:
4834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5839
Fragments arranged by size:
5839 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 3224 1186
Size: 1186 852 4026
Fragments arranged by size:
4026 1186 852
BseYI C'CCAG_C
Cuts at: 2068 5267 5763 2068
Size: 3199 496 2369
Fragments arranged by size:
3199 2369 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3334 1163
Size: 573 1598 3893
Fragments arranged by size:
3893 1598 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4125 4210 5281 4125
Size: 85 1071 4908
Fragments arranged by size:
4908 1071 85
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2485 3263 3383 4125 4210 5281 1391
Size: 399 695 778 120 742 85 1071 2174
Fragments arranged by size:
2174 1071 778 742 695 399 120 85
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 3419 844
Size: 1181 1394 3489
Fragments arranged by size:
3489 1394 1181
BsrBI CCG'CTC
Cuts at: 1394 1823 3951 1394
Size: 429 2128 3507
Fragments arranged by size:
3507 2128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6064
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 2458 1184
Size: 1274 4790
BstXI CCAn_nnnn'nTGG
Cuts at: 2536 2536
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 2490 2809 3105 3321 3394 3761 4146 4163
Size: 518 319 296 216 73 367 385 17
Cuts at: 4163 5615 5701 5713 6059 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 518 385 367 346 319 296
216 86 73 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4573
Fragments arranged by size:
4573 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5325
Fragments arranged by size:
5325 683 56
BtsI GCAGTG_nn'
Cuts at: 2404 3580 4374 4394 2404
Size: 1176 794 20 4074
Fragments arranged by size:
4074 1176 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 2172 3368 2172
Size: 1196 4868
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2296 2748 3511
Size: 54 1425 50 275 18 466 452 763
Cuts at: 3511 4400 6029 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 763 466 452 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3994 1353
Size: 2641 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4651 5025 5171 387
Size: 1064 138 3062 374 146 1280
Fragments arranged by size:
3062 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2996 3053 3096 4127 4724 5511
Size: 393 575 789 57 43 1031 597 787
Cuts at: 5511 5779 1239
Size: 268 1524
Fragments arranged by size:
1524 1031 789 787 597 575 393 268
57 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2199 2357 2660 3068 2199
Size: 158 303 408 5195
Fragments arranged by size:
5195 408 303 158
EcoO109I rG'GnC_Cy
Cuts at: 2442 2611 3214 2442
Size: 169 603 5292
Fragments arranged by size:
5292 603 169
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 5211 1678
Size: 3533 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2724 2756 4705
Size: 32 135 32 506 32 614 32 1949
Cuts at: 4705 4737 4779 4811 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1949 614 506 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 2548 3408 3654 669
Size: 988 281 382 228 860 246 3079
Fragments arranged by size:
3079 988 860 382 281 246 228
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5178 5362 1305
Size: 3873 184 2007
Fragments arranged by size:
3873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2298 2750 10
Size: 54 1425 343 466 452 3324
Fragments arranged by size:
3324 1425 466 452 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2838 4022 4381 4540 518
Size: 648 1672 1184 359 159 2042
Fragments arranged by size:
2042 1672 1184 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3223 3480 4157 5305 5550 1606
Size: 1617 257 677 1148 245 2120
Fragments arranged by size:
2120 1617 1148 677 257 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4573
Fragments arranged by size:
4573 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NsiI A_TGCA'T
Cuts at: 2284 2284
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2063 3422 4967 1132
Size: 97 834 1359 1545 2229
Fragments arranged by size:
2229 1545 1359 834 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 2059 4963 2059
Size: 2904 3160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6064
PfoI T'CCnGG_A
Cuts at: 2535 2949 3069 2535
Size: 414 120 5530
Fragments arranged by size:
5530 414 120
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2442 3214 2442
Size: 772 5292
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PstI C_TGCA'G
Cuts at: 830 2022 2043 2478 2512 2790 3416 830
Size: 1192 21 435 34 278 626 3478
Fragments arranged by size:
3478 1192 626 435 278 34 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 2318 3406 1936
Size: 382 1088 4594
Fragments arranged by size:
4594 1088 382
SbfI CC_TGCA'GG
Cuts at: 2022 2478 3416 2022
Size: 456 938 4670
Fragments arranged by size:
4670 938 456
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 2018 2039 2474 2508 2728 2786 3412
Size: 219 973 21 435 34 220 58 626
Cuts at: 3412 4547 5228 5419 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 973 681 626 435 220 219
191 58 34 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2992 4168 5069 5331 820
Size: 197 926 1049 1176 901 262 1553
Fragments arranged by size:
1553 1176 1049 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 2780 1517
Size: 1263 4801
StyI C'CwwG_G
Cuts at: 513 1065 2004 3503 513
Size: 552 939 1499 3074
Fragments arranged by size:
3074 1499 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 3050 4091 4108 4261 4446 4891
Size: 1255 897 573 1041 17 153 185 445
Cuts at: 4891 325
Size: 1498
Fragments arranged by size:
1498 1255 1041 897 573 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4310 6022 96
Size: 274 80 33 51 1443 2333 1712 138
Fragments arranged by size:
2333 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6024 6056 6024
Size: 32 6032
Tth111I GACn'n_nGTC
Cuts at: 1316 2210 2543 3198 1316
Size: 894 333 655 4182
Fragments arranged by size:
4182 894 655 333
XbaI T'CTAG_A
Cuts at: 3265 3400 3265
Size: 135 5929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3148 3244 3148
Size: 96 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6064
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 4193 4193
Size: 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI