Restriction map for
pFN21AB6665
(Circular) (Six-base) MAPSORT of: pFN21AB6665.seq Check: 8187 from: 1 to: 5977
pFN21AB6665 5977 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2456 2465 3197 4040 5424 5692 1632
Size: 824 9 732 843 1384 268 1917
Fragments arranged by size:
1917 1384 843 824 732 268 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 2052 2632 2761 3720 5292 2052
Size: 580 129 959 1572 2737
Fragments arranged by size:
2737 1572 959 580 129
ApaI G_GGCC'C
Cuts at: 2188 2219 2188
Size: 31 5946
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 3286 3512 3664 5773 1493
Size: 1793 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 3028 3302 1870
Size: 73 1085 274 4545
Fragments arranged by size:
4545 1085 274 73
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2100 3005 3207 3272 3298
Size: 325 217 627 313 905 202 65 26
Cuts at: 3298 4753 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 905 627 325 313 217 202
65 26
BanII G_rGCy'C
Cuts at: 1391 2188 2219 2963 3296 1391
Size: 797 31 744 333 4072
Fragments arranged by size:
4072 797 744 333 31
BbeI G_GCGC'C
Cuts at: 3276 3276
Size: 5977
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2504 2612 928
Size: 971 605 108 4293
Fragments arranged by size:
4293 971 605 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2597 3237 4588
Size: 107 122 71 631 605 925 640 1351
Cuts at: 4588 136
Size: 1525
Fragments arranged by size:
1525 1351 925 640 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 2268 2276 3149 3394 2268
Size: 8 873 245 4851
Fragments arranged by size:
4851 873 245 8
Bme1580I G_kGCm'C
Cuts at: 1790 2103 2188 2219 3115 4038 5194 1790
Size: 313 85 31 896 923 1156 2573
Fragments arranged by size:
2573 1156 923 896 313 85 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2191 2215 3016
Size: 416 53 83 399 24 774 24 801
Cuts at: 3016 3708 4666 442
Size: 692 958 1753
Fragments arranged by size:
1753 958 801 774 692 416 399 83
53 24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1995 2046 2255 2312 4637 1239
Size: 756 51 209 57 2325 2579
Fragments arranged by size:
2579 2325 756 209 57 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4102 4967 5265 4102
Size: 865 298 4814
Fragments arranged by size:
4814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2684 4640 882
Size: 617 1185 1956 2219
Fragments arranged by size:
2219 1956 1185 617
BsaAI yAC'GTr
Cuts at: 493 1204 3259 3768 493
Size: 711 2055 509 2702
Fragments arranged by size:
2702 2055 711 509
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3273 3784 4166 4801
Size: 53 83 186 936 1740 511 382 635
Cuts at: 4801 275
Size: 1451
Fragments arranged by size:
1740 1451 936 635 511 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5752
Fragments arranged by size:
5752 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2918 3071 3182 3203 1186
Size: 1732 153 111 21 3960
Fragments arranged by size:
3960 1732 153 111 21
BseYI C'CCAG_C
Cuts at: 2166 2371 5180 5676 2166
Size: 205 2809 496 2467
Fragments arranged by size:
2809 2467 496 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2270 1163
Size: 573 534 4870
Fragments arranged by size:
4870 573 534
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2400 3427 4188 5216 1540
Size: 275 186 399 1027 761 1028 2301
Fragments arranged by size:
2301 1028 1027 761 399 275 186
BsiHKAI G_wGCw'C
Cuts at: 4038 4123 5194 4038
Size: 85 1071 4821
Fragments arranged by size:
4821 1071 85
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
BsmBI CGTCTCn'nnnn_
Cuts at: 2548 2548
Size: 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2188 2219 2963 3115 3296 4038
Size: 399 313 85 31 744 152 181 742
Cuts at: 4038 4123 5194 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 744 742 399 313 181 152
85 85 31
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 3332 844
Size: 1858 630 3489
Fragments arranged by size:
3489 1858 630
BsrBI CCG'CTC
Cuts at: 1394 1823 2272 2329 3864 1394
Size: 429 449 57 1535 3507
Fragments arranged by size:
3507 1535 449 429 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 4472 4646 65
Size: 1183 3224 174 1396
Fragments arranged by size:
3224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 2361 4621 4808 1700
Size: 110 293 258 2260 187 2869
Fragments arranged by size:
2869 2260 293 258 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 2140 2280 2335 1925
Size: 215 140 55 5567
Fragments arranged by size:
5567 215 140 55
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5977
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 2971 1184
Size: 1787 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 3259 3259
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 2842 3307 3674 4059 4076 5528 5614 5626
Size: 870 465 367 385 17 1452 86 12
Cuts at: 5626 5972 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 870 465 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2038 2038
Size: 5977
BtgI C'CryG_G
Cuts at: 513 1065 2004 2205 2866 513
Size: 552 939 201 661 3624
Fragments arranged by size:
3624 939 661 552 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2630 487
Size: 683 56 1404 3834
Fragments arranged by size:
3834 1404 683 56
BtsI GCAGTG_nn'
Cuts at: 2782 3493 4287 4307 2782
Size: 711 794 20 4452
Fragments arranged by size:
4452 794 711 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2540 4984 809
Size: 507 1224 2444 1802
Fragments arranged by size:
2444 1802 1224 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 2880 2891
Size: 54 1425 50 275 18 567 483 11
Cuts at: 2891 2953 3130 3229 3424 4313 5942 8
Size: 62 177 99 195 889 1629 43
Fragments arranged by size:
1629 1425 889 567 483 275 195 177
99 62 54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2397 3424 1537
Size: 275 585 1027 4090
Fragments arranged by size:
4090 1027 585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2432 2832 3066 3907 1353
Size: 657 422 400 234 841 3423
Fragments arranged by size:
3423 841 657 422 400 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2046 2255 2312 2456 2465 3197
Size: 393 363 51 209 57 144 9 732
Cuts at: 3197 4040 4637 5424 5692 1239
Size: 843 597 787 268 1524
Fragments arranged by size:
1524 843 787 732 597 393 363 268
209 144 57 51 9
EcoO109I rG'GnC_Cy
Cuts at: 2116 2215 2216 3033 2116
Size: 99 1 817 5060
Fragments arranged by size:
5060 817 99 1
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FspI TGC'GCA
Cuts at: 2561 4483 2561
Size: 1922 4055
HaeII r_GCGC'y
Cuts at: 1678 2541 2713 3276 5124 1678
Size: 863 172 563 1848 2531
Fragments arranged by size:
2531 1848 863 563 172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2599 2631 2894 2926 3047
Size: 32 135 32 1027 32 263 32 121
Cuts at: 3047 3079 4618 4650 4692 4724 1373
Size: 32 1539 32 42 32 2626
Fragments arranged by size:
2626 1539 1027 263 135 121 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KasI G'GCGC_C
Cuts at: 3272 3272
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5091 5275 1305
Size: 3786 184 2007
Fragments arranged by size:
3786 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2882 2893 3132 10
Size: 54 1425 343 1050 11 239 2855
Fragments arranged by size:
2855 1425 1050 343 239 54 11
MslI CAynn'nnrTG
Cuts at: 518 1166 2882 3935 4294 4453 518
Size: 648 1716 1053 359 159 2042
Fragments arranged by size:
2042 1716 1053 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 2127 2347 2557 2716 3393 4070 5218
Size: 480 41 220 210 159 677 677 1148
Cuts at: 5218 5463 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 677 480 245 220 210
159 41
NarI GG'CG_CC
Cuts at: 3273 3273
Size: 5977
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4486
Fragments arranged by size:
4486 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2252 3335 4880 1132
Size: 97 1023 1083 1545 2229
Fragments arranged by size:
2229 1545 1083 1023 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2173 2761 2889 1108
Size: 1065 588 128 4196
Fragments arranged by size:
4196 1065 588 128
PfoI T'CCnGG_A
Cuts at: 2133 2133
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2116 3033 2116
Size: 917 5060
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspOMI G'GGCC_C
Cuts at: 2184 2215 2184
Size: 31 5946
PstI C_TGCA'G
Cuts at: 830 2571 3329 830
Size: 1741 758 3478
Fragments arranged by size:
3478 1741 758
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2127 2557 2127
Size: 430 5547
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SanDI GG'GwC_CC
Cuts at: 3033 3033
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SexAI A'CCwGG_T
Cuts at: 2118 2118
Size: 5977
SfcI C'TryA_G
Cuts at: 826 1045 2068 2567 3325 4460 5141 5332 826
Size: 219 1023 499 758 1135 681 191 1471
Fragments arranged by size:
1471 1135 1023 758 681 499 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3237 3237
Size: 5977
SfoI GGC'GCC
Cuts at: 3274 3274
Size: 5977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3030 3304 1872
Size: 1158 274 4545
Fragments arranged by size:
4545 1158 274
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4081 4982 5244 820
Size: 197 926 2138 901 262 1553
Fragments arranged by size:
2138 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 2252 3335 1132
Size: 1120 1083 3774
Fragments arranged by size:
3774 1120 1083
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 2036 2243 2684 1517
Size: 519 207 441 4810
Fragments arranged by size:
4810 519 441 207
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 2913 3211 3416 513
Size: 552 939 588 321 298 205 3074
Fragments arranged by size:
3074 939 588 552 321 298 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2879 4004 4021 4174 4359 4804 325
Size: 1255 1299 1125 17 153 185 445 1498
Fragments arranged by size:
1498 1299 1255 1125 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4223 5935 96
Size: 274 80 33 51 1443 2246 1712 138
Fragments arranged by size:
2246 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3044 3076 5937 5969 3044
Size: 32 2861 32 3052
Fragments arranged by size:
3052 2861 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2120 1316
Size: 804 5173
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3224 3224
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3028 3302 1870
Size: 1158 274 4545
Fragments arranged by size:
4545 1158 274
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SapI SrfI
SwaI