Restriction map for
pFN21AB6664
(Circular) (Six-base) MAPSORT of: pFN21AB6664.seq Check: 122 from: 1 to: 6061
pFN21AB6664 6061 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 2190 4188 4561 1563
Size: 627 1998 373 3063
Fragments arranged by size:
3063 1998 627 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2463 4124 5508 5776 1632
Size: 831 1661 1384 268 1917
Fragments arranged by size:
1917 1661 1384 831 268
AflII C'TTAA_G
Cuts at: 820 1017 2168 820
Size: 197 1151 4713
Fragments arranged by size:
4713 1151 197
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 2879 1166
Size: 1713 4348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 2513 2770 3221 3804 5376 2513
Size: 257 451 583 1572 3198
Fragments arranged by size:
3198 1572 583 451 257
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 2077 2365 2545 2640 3370 3596 3748 5857
Size: 584 288 180 95 730 226 152 2109
Cuts at: 5857 1493
Size: 1697
Fragments arranged by size:
2109 1697 730 584 288 226 180 152
95
AseI AT'TA_AT
Cuts at: 160 1032 2133 4615 160
Size: 872 1101 2482 1606
Fragments arranged by size:
2482 1606 1101 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 3386 1870
Size: 73 89 1354 4545
Fragments arranged by size:
4545 1354 89 73
AvrII C'CTAG_G
Cuts at: 2572 2572
Size: 6061
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2814 2847 2814
Size: 33 6028
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 3382 4837 618
Size: 325 217 627 255 1340 1455 1842
Fragments arranged by size:
1842 1455 1340 627 325 255 217
BanII G_rGCy'C
Cuts at: 1391 2012 3128 3380 1391
Size: 621 1116 252 4072
Fragments arranged by size:
4072 1116 621 252
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3150 928
Size: 971 1251 3839
Fragments arranged by size:
3839 1251 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2446 6056 2446
Size: 3610 2451
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2045 4122 5278 1790
Size: 255 2077 1156 2573
Fragments arranged by size:
2573 2077 1156 255
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3299 3792 4750
Size: 416 53 83 399 24 1882 493 958
Cuts at: 4750 442
Size: 1753
Fragments arranged by size:
1882 1753 958 493 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 4721 1239
Size: 1133 2349 2579
Fragments arranged by size:
2579 2349 1133
Bpu10I CC'TnA_GC
Cuts at: 3142 3142
Size: 6061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2240 4724 882
Size: 617 741 2484 2219
Fragments arranged by size:
2484 2219 741 617
BsaAI yAC'GTr
Cuts at: 493 1204 3852 493
Size: 711 2648 2702
Fragments arranged by size:
2702 2648 711
BsaBI GATnn'nnATC
Cuts at: 2213 6055 2213
Size: 3842 2219
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3248 3868 4250 4885
Size: 53 83 186 936 1715 620 382 635
Cuts at: 4885 275
Size: 1451
Fragments arranged by size:
1715 1451 936 635 620 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3209 3239 1178
Size: 30 165 30 1806 30 4000
Fragments arranged by size:
4000 1806 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2002 3217 1186
Size: 816 1215 4030
Fragments arranged by size:
4030 1215 816
BseYI C'CCAG_C
Cuts at: 2971 5264 5760 2971
Size: 2293 496 3272
Fragments arranged by size:
3272 2293 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3128 4122 4207 5278 3128
Size: 994 85 1071 3911
Fragments arranged by size:
3911 1071 994 85
BsmI GAATG_Cn'
Cuts at: 2301 3572 3665 2301
Size: 1271 93 4697
Fragments arranged by size:
4697 1271 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2045 3128 3380 4122 4207 5278
Size: 399 222 33 1083 252 742 85 1071
Cuts at: 5278 1391
Size: 2174
Fragments arranged by size:
2174 1083 1071 742 399 252 222 85
33
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 2430 3950 5680 2430
Size: 1520 1730 2811
Fragments arranged by size:
2811 1730 1520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2375 3416 844
Size: 1531 1041 3489
Fragments arranged by size:
3489 1531 1041
BsrBI CCG'CTC
Cuts at: 1394 1823 3948 1394
Size: 429 2125 3507
Fragments arranged by size:
3507 2125 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3066 4556 4730 65
Size: 1183 1818 1490 174 1396
Fragments arranged by size:
1818 1490 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4705 4892 1700
Size: 110 2895 187 2869
Fragments arranged by size:
2895 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2499 96
Size: 2403 3658
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3221 794
Size: 2427 3634
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 2028 2173 2446 3391 3758 4143 4160 5612
Size: 56 145 273 945 367 385 17 1452
Cuts at: 5612 5698 5710 6056 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 945 385 367 346 273 145
86 56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4570
Fragments arranged by size:
4570 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 3255 3577 4371 4391 3255
Size: 322 794 20 4925
Fragments arranged by size:
4925 794 322 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3259 3294 529
Size: 35 2695 35 3296
Fragments arranged by size:
3296 2695 35 35
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DraIII CAC_nnn'GTG
Cuts at: 2402 2402
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2278 3508 4397
Size: 54 1425 50 275 18 448 1230 889
Cuts at: 4397 6026 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1230 889 448 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2652 3039 3133 3991 1353
Size: 1299 387 94 858 3423
Fragments arranged by size:
3423 1299 858 387 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3126 3126
Size: 6061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2463 4124 4721 5508 5776 1239
Size: 393 740 91 1661 597 787 268 1524
Fragments arranged by size:
1661 1524 787 740 597 393 268 91
EcoO109I rG'GnC_Cy
Cuts at: 3265 3265
Size: 6061
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 5208 1678
Size: 3530 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 3140 3172 3209
Size: 32 135 32 773 32 763 32 37
Cuts at: 3209 3241 4702 4734 4776 4808 1373
Size: 32 1461 32 42 32 2626
Fragments arranged by size:
2626 1461 773 763 135 42 37 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5175 5359 1305
Size: 3870 184 2007
Fragments arranged by size:
3870 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4239
Fragments arranged by size:
4239 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 4019 4378 4537 518
Size: 648 1713 1140 359 159 2042
Fragments arranged by size:
2042 1713 1140 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2710 2804 3477 4154 5302 5547 1606
Size: 1104 94 673 677 1148 245 2120
Fragments arranged by size:
2120 1148 1104 677 673 245 94
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4570
Fragments arranged by size:
4570 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2348 3419 4964 1132
Size: 97 1119 1071 1545 2229
Fragments arranged by size:
2229 1545 1119 1071 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2996 1108
Size: 1888 4173
PfoI T'CCnGG_A
Cuts at: 2650 2650
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2252 1613
Size: 639 5422
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PstI C_TGCA'G
Cuts at: 830 2315 2377 3136 3413 830
Size: 1485 62 759 277 3478
Fragments arranged by size:
3478 1485 759 277 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 2710 2804 2710
Size: 94 5967
SacI G_AGCT'C
Cuts at: 3128 3128
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SapI GCTCTTCn'nnn_
Cuts at: 3133 3133
Size: 6061
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SexAI A'CCwGG_T
Cuts at: 2911 2911
Size: 6061
SfcI C'TryA_G
Cuts at: 826 1045 2218 2311 2373 2751 3132 3409 4544
Size: 219 1173 93 62 378 381 277 1135
Cuts at: 4544 5225 5416 826
Size: 681 191 1471
Fragments arranged by size:
1471 1173 1135 681 381 378 277 219
191 93 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 2034 3388 1872
Size: 162 1354 4545
Fragments arranged by size:
4545 1354 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 4165 5066 5328 820
Size: 197 926 225 1997 901 262 1553
Fragments arranged by size:
1997 1553 926 901 262 225 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2004 2572 3111 3261 3500 513
Size: 552 939 568 539 150 239 3074
Fragments arranged by size:
3074 939 568 552 539 239 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4088 4105 4258 4443 4888 325
Size: 1255 2508 17 153 185 445 1498
Fragments arranged by size:
2508 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2499 2822 4307
Size: 274 80 33 51 1443 522 323 1485
Cuts at: 4307 6019 96
Size: 1712 138
Fragments arranged by size:
1712 1485 1443 522 323 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6021 6053 6021
Size: 32 6029
Tth111I GACn'n_nGTC
Cuts at: 1316 3287 1316
Size: 1971 4090
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2268 2550 2268
Size: 282 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 2032 3386 1870
Size: 162 1354 4545
Fragments arranged by size:
4545 1354 162
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI