Restriction map for
pFN21AB6663
(Circular) (Six-base) MAPSORT of: pFN21AB6663.seq Check: 2711 from: 1 to: 5977
pFN21AB6663 5977 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4040 5424 5692 1632
Size: 2408 1384 268 1917
Fragments arranged by size:
2408 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 2120 4706 2120
Size: 2586 3391
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2088 2120 2591 2623 2088
Size: 32 471 32 5442
Fragments arranged by size:
5442 471 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2990 3720 5292 2990
Size: 730 1572 3675
Fragments arranged by size:
3675 1572 730
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 2183 3286 3512 3664 5773 1493
Size: 690 1103 226 152 2109 1697
Fragments arranged by size:
2109 1697 1103 690 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2445 4531 160
Size: 872 1413 2086 1606
Fragments arranged by size:
2086 1606 1413 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 3063 3246 3302 1870
Size: 73 1120 183 56 4545
Fragments arranged by size:
4545 1120 183 73 56
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3298 4753 618
Size: 325 217 627 1511 1455 1842
Fragments arranged by size:
1842 1511 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2761 3296 1391
Size: 1370 535 4072
Fragments arranged by size:
4072 1370 535
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5006
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2766 3149 3869 5079 2766
Size: 383 720 1210 3664
Fragments arranged by size:
3664 1210 720 383
BclI T'GATC_A
Cuts at: 1548 2232 1548
Size: 684 5293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2955 4588 136
Size: 107 122 71 631 605 1283 1633 1525
Fragments arranged by size:
1633 1525 1283 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 2569 3394 2569
Size: 825 5152
Bme1580I G_kGCm'C
Cuts at: 1790 4038 5194 1790
Size: 2248 1156 2573
Fragments arranged by size:
2573 2248 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2735 3708 4666
Size: 416 53 83 399 24 1318 973 958
Cuts at: 4666 442
Size: 1753
Fragments arranged by size:
1753 1318 973 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3284
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2775 4637 1239
Size: 851 685 1862 2579
Fragments arranged by size:
2579 1862 851 685
Bpu10I CC'TnA_GC
Cuts at: 2959 2959
Size: 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2209 4102 4967 5265 2209
Size: 1893 865 298 2921
Fragments arranged by size:
2921 1893 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2053 3768 493
Size: 711 849 1715 2702
Fragments arranged by size:
2702 1715 849 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3784 4166 4801 275
Size: 53 83 186 936 2251 382 635 1451
Fragments arranged by size:
2251 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2088 2118 2591 2621 3151
Size: 30 165 30 685 30 473 30 530
Cuts at: 3151 3181 1178
Size: 30 3974
Fragments arranged by size:
3974 685 530 473 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2640 2709 3033 3123 1186
Size: 1454 69 324 90 4040
Fragments arranged by size:
4040 1454 324 90 69
BseYI C'CCAG_C
Cuts at: 2779 5180 5676 2779
Size: 2401 496 3080
Fragments arranged by size:
3080 2401 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5404
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4038 4123 5194 4038
Size: 85 1071 4821
Fragments arranged by size:
4821 1071 85
BsmI GAATG_Cn'
Cuts at: 3030 3488 3581 3030
Size: 458 93 5426
Fragments arranged by size:
5426 458 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2761 3296 4038 4123 5194 1391
Size: 399 971 535 742 85 1071 2174
Fragments arranged by size:
2174 1071 971 742 535 399 85
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 2223 3866 5596 2223
Size: 1643 1730 2604
Fragments arranged by size:
2604 1730 1643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3332 844
Size: 2488 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 2605 3864 1394
Size: 429 206 576 1259 3507
Fragments arranged by size:
3507 1259 576 429 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 4472 4646 65
Size: 1183 3224 174 1396
Fragments arranged by size:
3224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3121 794
Size: 2327 3650
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 3092 1184
Size: 1908 4069
BstXI CCAn_nnnn'nTGG
Cuts at: 2171 2674 2171
Size: 503 5474
BstYI r'GATC_y
Cuts at: 1972 3080 3307 3674 4059 4076 5528 5614 5626
Size: 1108 227 367 385 17 1452 86 12
Cuts at: 5626 5972 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1108 385 367 346 227 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3273 3273
Size: 5977
BtgI C'CryG_G
Cuts at: 513 1065 2004 2563 2944 3087 3151 513
Size: 552 939 559 381 143 64 3339
Fragments arranged by size:
3339 939 559 552 381 143 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 3493 4287 4307 3493
Size: 794 20 5163
Fragments arranged by size:
5163 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DraIII CAC_nnn'GTG
Cuts at: 2900 2900
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2862 4984 809
Size: 507 1546 2122 1802
Fragments arranged by size:
2122 1802 1546 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 3230 3424
Size: 54 1425 50 275 18 325 1075 194
Cuts at: 3424 4313 5942 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1075 889 325 275 194 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3907 1353
Size: 2554 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2775 4040 4637 5424 5692 1239
Size: 393 458 685 1265 597 787 268 1524
Fragments arranged by size:
1524 1265 787 685 597 458 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2415 2706 2415
Size: 291 5686
EcoO109I rG'GnC_Cy
Cuts at: 2059 2407 3068 3101 3200 3263 2059
Size: 348 661 33 99 63 4773
Fragments arranged by size:
4773 661 348 99 63 33
EcoRI G'AATT_C
Cuts at: 2183 3286 3664 2183
Size: 1103 378 4496
Fragments arranged by size:
4496 1103 378
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 2370 2563 5124 1678
Size: 692 193 2561 2531
Fragments arranged by size:
2561 2531 692 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2106 2138 2896 2928 4618
Size: 32 135 32 534 32 758 32 1690
Cuts at: 4618 4650 4692 4724 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1690 758 534 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2125 3321 3567 669
Size: 988 281 187 1196 246 3079
Fragments arranged by size:
3079 1196 988 281 246 187
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 2478 3576 2478
Size: 1098 4879
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5091 5275 1305
Size: 3786 184 2007
Fragments arranged by size:
3786 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2157 10
Size: 54 1425 343 325 3830
Fragments arranged by size:
3830 1425 343 325 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 2228 3935 4294 4453 518
Size: 648 966 96 1707 359 159 2042
Fragments arranged by size:
2042 1707 966 648 359 159 96
MspA1I CmG'CkG
Cuts at: 1606 2086 2632 3196 3393 4070 5218 5463 1606
Size: 480 546 564 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 564 546 480 245 197
NcoI C'CATG_G
Cuts at: 513 1065 2004 2563 2944 3087 3151 513
Size: 552 939 559 381 143 64 3339
Fragments arranged by size:
3339 939 559 552 381 143 64
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2137 2716 3058 3335 4880 1132
Size: 97 908 579 342 277 1545 2229
Fragments arranged by size:
2229 1545 908 579 342 277 97
PasI CC'CwG_GG
Cuts at: 1348 2846 1348
Size: 1498 4479
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2753 2900 2990 3020 3185 1108
Size: 1645 147 90 30 165 3900
Fragments arranged by size:
3900 1645 165 147 90 30
PfoI T'CCnGG_A
Cuts at: 2752 3034 2752
Size: 282 5695
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2088 2120 2591 2623 4046 4078 5559 5591 2088
Size: 32 471 32 1423 32 1481 32 2474
Fragments arranged by size:
2474 1481 1423 471 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2407 3101 2407
Size: 694 5283
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspXI vC'TCGA_Gb
Cuts at: 3246 3246
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2818 3329 830
Size: 1988 511 3478
Fragments arranged by size:
3478 1988 511
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2632 2632
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SanDI GG'GwC_CC
Cuts at: 2407 2407
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2814 3325 4460 5141 5332 826
Size: 219 1769 511 1135 681 191 1471
Fragments arranged by size:
1769 1471 1135 681 511 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2188 3246 4081 4982 5244 820
Size: 197 926 245 1058 835 901 262 1553
Fragments arranged by size:
1553 1058 926 901 835 262 245 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 2716 3058 3335 1132
Size: 1584 342 277 3774
Fragments arranged by size:
3774 1584 342 277
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2004 2164 2550 2563 2944 3087 3151
Size: 552 939 160 386 13 381 143 64
Cuts at: 3151 3416 513
Size: 265 3074
Fragments arranged by size:
3074 939 552 386 381 265 160 143
64 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2144 2901 3111 3229 4004 4021 4174
Size: 1255 564 757 210 118 775 17 153
Cuts at: 4174 4359 4804 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 775 757 564 445 210 185
153 118 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4223 5935 96
Size: 274 80 33 51 1443 2246 1712 138
Fragments arranged by size:
2246 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3151 3183 5937 5969 3151
Size: 32 2754 32 3159
Fragments arranged by size:
3159 2754 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 2951 3158 2050
Size: 901 207 4869
Fragments arranged by size:
4869 901 207
XhoI C'TCGA_G
Cuts at: 1943 3246 1943
Size: 1303 4674
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 2100 2583 4106 2100
Size: 483 1523 3971
Fragments arranged by size:
3971 1523 483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI