Restriction map for
pFN21AB6658
(Circular) (Six-base) MAPSORT of: pFN21AB6658.seq Check: 6036 from: 1 to: 6058
pFN21AB6658 6058 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2878 2878
Size: 6058
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2106 3027 3114 3868
Size: 53 83 186 936 570 921 87 754
Cuts at: 3868 278
Size: 2468
Fragments arranged by size:
2468 936 921 754 570 186 87 83
53
AccI GT'mk_AC
Cuts at: 1937 3027 3401 1937
Size: 1090 374 4594
Fragments arranged by size:
4594 1090 374
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3093 4121 5505 5773 1632
Size: 1461 1028 1384 268 1917
Fragments arranged by size:
1917 1461 1384 1028 268
AfeI AGC'GCT
Cuts at: 3078 3078
Size: 6058
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 4957 5934 1203
Size: 3754 977 1327
Fragments arranged by size:
3754 1327 977
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 2062 3115 4787 2062
Size: 1053 1672 3333
Fragments arranged by size:
3333 1672 1053
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 2222
Size: 32 6026
AlwNI CAG_nnn'CTG
Cuts at: 2482 2698 3801 5373 2482
Size: 216 1103 1572 3167
Fragments arranged by size:
3167 1572 1103 216
ApaI G_GGCC'C
Cuts at: 2036 2476 2036
Size: 440 5618
ApaLI G'TGCA_C
Cuts at: 3251 4115 5271 3251
Size: 864 1156 4038
Fragments arranged by size:
4038 1156 864
ApoI r'AATT_y
Cuts at: 1493 3367 3593 3745 5854 1493
Size: 1874 226 152 2109 1697
Fragments arranged by size:
2109 1874 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2450 2670 3084 3287 3383 1870
Size: 73 70 437 220 414 203 96 4545
Fragments arranged by size:
4545 437 414 220 203 96 73 70
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 3160 3379 4834 618
Size: 325 217 627 516 857 219 1455 1842
Fragments arranged by size:
1842 1455 857 627 516 325 219 217
BanII G_rGCy'C
Cuts at: 1391 2036 2476 2535 2631 2802 3377 1391
Size: 645 440 59 96 171 575 4072
Fragments arranged by size:
4072 645 575 440 171 96 59
BbeI G_GCGC'C
Cuts at: 2307 2307
Size: 6058
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 2611 928
Size: 971 199 513 4375
Fragments arranged by size:
4375 971 513 199
BbvCI CC'TCA_GC
Cuts at: 2441 2895 2441
Size: 454 5604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4249 4283 1903
Size: 34 2312 34 3678
Fragments arranged by size:
3678 2312 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3950 5160 3950
Size: 1210 4848
BclI T'GATC_A
Cuts at: 1548 2313 1548
Size: 765 5293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4669 136
Size: 107 122 71 631 605 2997 1525
Fragments arranged by size:
2997 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2388 6053 2388
Size: 3665 2393
BlpI GC'TnA_GC
Cuts at: 2084 3475 2084
Size: 1391 4667
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2193 2476 3165 3242 3255 4119 5275
Size: 246 157 283 689 77 13 864 1156
Cuts at: 5275 1790
Size: 2573
Fragments arranged by size:
2573 1156 864 689 283 246 157 77
13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2563 3789 4747
Size: 416 53 83 399 24 1146 1226 958
Cuts at: 4747 442
Size: 1753
Fragments arranged by size:
1753 1226 1146 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 3343 2696
Size: 647 5411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2843 2933 3254 3313 4718 1239
Size: 1604 90 321 59 1405 2579
Fragments arranged by size:
2579 1604 1405 321 90 59
Bpu10I CC'TnA_GC
Cuts at: 2441 2529 2895 2441
Size: 88 366 5604
Fragments arranged by size:
5604 366 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4183 5048 5346 4183
Size: 865 298 4895
Fragments arranged by size:
4895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3289 4721 882
Size: 617 1790 1432 2219
Fragments arranged by size:
2219 1790 1432 617
BsaAI yAC'GTr
Cuts at: 493 1204 2113 3849 493
Size: 711 909 1736 2702
Fragments arranged by size:
2702 1736 909 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2304 3024 3111
Size: 53 83 186 936 570 201 720 87
Cuts at: 3111 3207 3865 4247 4882 275
Size: 96 658 382 635 1451
Fragments arranged by size:
1451 936 720 658 635 570 382 201
186 96 87 83 53
BsaWI w'CCGG_w
Cuts at: 4491 4889 5163 5310 5721 4491
Size: 398 274 147 411 4828
Fragments arranged by size:
4828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2120 2147 2150 1178
Size: 30 165 30 714 3 27 3 5086
Fragments arranged by size:
5086 714 165 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2141 2585 1186
Size: 907 48 444 4659
Fragments arranged by size:
4659 907 444 48
BseYI C'CCAG_C
Cuts at: 2593 3245 5261 5757 2593
Size: 652 2016 496 2894
Fragments arranged by size:
2894 2016 652 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2399 2669 3298 1163
Size: 573 663 270 629 3923
Fragments arranged by size:
3923 663 629 573 270
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3357 3508 4269 5297 1540
Size: 275 186 1356 151 761 1028 2301
Fragments arranged by size:
2301 1356 1028 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 2802 3255 4119 4204 5275 2802
Size: 453 864 85 1071 3585
Fragments arranged by size:
3585 1071 864 453 85
BsmI GAATG_Cn'
Cuts at: 3569 3662 3569
Size: 93 5965
BsmBI CGTCTCn'nnnn_
Cuts at: 2210 2210
Size: 6058
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2193 2476 2535 2631 2802 3165
Size: 399 246 157 283 59 96 171 363
Cuts at: 3165 3242 3255 3377 4119 4204 5275 1391
Size: 77 13 122 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 283 246 171
157 122 96 85 77 59 13
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 3947 5677 3947
Size: 1730 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2878 3413 844
Size: 2034 535 3489
Fragments arranged by size:
3489 2034 535
BsrBI CCG'CTC
Cuts at: 1394 1823 2259 3945 1394
Size: 429 436 1686 3507
Fragments arranged by size:
3507 1686 436 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4553 4727 65
Size: 1183 3305 174 1396
Fragments arranged by size:
3305 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2409 3209 4702 4889 1700
Size: 110 599 800 1493 187 2869
Fragments arranged by size:
2869 1493 800 599 187 110
BsrGI T'GTAC_A
Cuts at: 96 2784 96
Size: 2688 3370
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 2522 4118 5130 2522
Size: 1596 1012 3450
Fragments arranged by size:
3450 1596 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2374 2698 3225 794
Size: 1580 324 527 3627
Fragments arranged by size:
3627 1580 527 324
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstXI CCAn_nnnn'nTGG
Cuts at: 2044 2152 2044
Size: 108 5950
BstYI r'GATC_y
Cuts at: 1972 2388 2709 3388 3755 4140 4157 5609 5695
Size: 416 321 679 367 385 17 1452 86
Cuts at: 5695 5707 6053 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 679 416 385 367 346 321
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3197 3197
Size: 6058
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2234 2298 2488 2760 2769
Size: 552 939 168 62 64 190 272 9
Cuts at: 2769 513
Size: 3802
Fragments arranged by size:
3802 939 552 272 190 168 64 62
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2863 487
Size: 683 56 1637 3682
Fragments arranged by size:
3682 1637 683 56
BtsI GCAGTG_nn'
Cuts at: 2138 3574 4368 4388 2138
Size: 1436 794 20 3808
Fragments arranged by size:
3808 1436 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 3362 3362
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2224 5065 809
Size: 507 908 2841 1802
Fragments arranged by size:
2841 1802 908 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2205 2502
Size: 54 1425 50 275 18 285 90 297
Cuts at: 2502 2652 2805 2943 3505 4394 6023 8
Size: 150 153 138 562 889 1629 43
Fragments arranged by size:
1629 1425 889 562 297 285 275 153
150 138 90 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3988 1353
Size: 2635 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2800 2800
Size: 6058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2843 2933 3093 3254 3313 4121 4718
Size: 393 1211 90 160 161 59 808 597
Cuts at: 4718 5505 5773 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1211 808 787 597 393 268 161
160 90 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2423 3196 2423
Size: 773 5285
EcoO109I rG'GnC_Cy
Cuts at: 2033 2472 2776 2938 3283 3310 2033
Size: 439 304 162 345 27 4781
Fragments arranged by size:
4781 439 345 304 162 27
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 2307 2753 3080 5205 1678
Size: 629 446 327 2125 2531
Fragments arranged by size:
2531 2125 629 446 327
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2417 2449 3101
Size: 32 135 32 476 32 337 32 652
Cuts at: 3101 3133 4699 4731 4773 4805 1373
Size: 32 1566 32 42 32 2626
Fragments arranged by size:
2626 1566 652 476 337 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KasI G'GCGC_C
Cuts at: 2303 2303
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 2685 3132 3204 5172 5356 1305
Size: 903 477 447 72 1968 184 2007
Fragments arranged by size:
2007 1968 903 477 447 184 72
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2654 2807 10
Size: 54 1425 343 285 537 153 3261
Fragments arranged by size:
3261 1425 537 343 285 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2547 2660 3274 4016 4375 4534 518
Size: 648 1381 113 614 742 359 159 2042
Fragments arranged by size:
2042 1381 742 648 614 359 159 113
MspA1I CmG'CkG
Cuts at: 1606 3474 4151 5299 5544 1606
Size: 1868 677 1148 245 2120
Fragments arranged by size:
2120 1868 1148 677 245
NaeI GCC'GGC
Cuts at: 2411 3211 2411
Size: 800 5258
NarI GG'CG_CC
Cuts at: 2304 2304
Size: 6058
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4567
Fragments arranged by size:
4567 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NgoMIV G'CCGG_C
Cuts at: 2409 3209 2409
Size: 800 5258
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 2905 3178 3223 3273 3416 4961 1132
Size: 97 1676 273 45 50 143 1545 2229
Fragments arranged by size:
2229 1676 1545 273 143 97 50 45
PasI CC'CwG_GG
Cuts at: 1348 2179 1348
Size: 831 5227
PciI A'CATG_T
Cuts at: 4957 4957
Size: 6058
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6058
PfoI T'CCnGG_A
Cuts at: 2066 2066
Size: 6058
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PmlI CAC'GTG
Cuts at: 2113 2113
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2776 2938 3283 2776
Size: 162 345 5551
Fragments arranged by size:
5551 345 162
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PspOMI G'GGCC_C
Cuts at: 2032 2472 2032
Size: 440 5618
PstI C_TGCA'G
Cuts at: 830 2283 2588 2703 3410 830
Size: 1453 305 115 707 3478
Fragments arranged by size:
3478 1453 707 305 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
SacI G_AGCT'C
Cuts at: 2802 2802
Size: 6058
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SanDI GG'GwC_CC
Cuts at: 2776 3283 2776
Size: 507 5551
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 2279 2584 2699 3406 4541 5222 5413
Size: 219 1234 305 115 707 1135 681 191
Cuts at: 5413 826
Size: 1471
Fragments arranged by size:
1471 1234 1135 707 681 305 219 191
115
SfoI GGC'GCC
Cuts at: 2305 2305
Size: 6058
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 2015 3385 1872
Size: 143 1370 4545
Fragments arranged by size:
4545 1370 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2670 4162 5063 5325 820
Size: 197 926 727 1492 901 262 1553
Fragments arranged by size:
1553 1492 926 901 727 262 197
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3223 3416 1132
Size: 2091 193 3774
Fragments arranged by size:
3774 2091 193
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 3191 1517
Size: 1674 4384
StyI C'CwwG_G
Cuts at: 513 1065 2004 2808 3497 513
Size: 552 939 804 689 3074
Fragments arranged by size:
3074 939 804 689 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4085 4102 4255 4440 4885 325
Size: 1255 2505 17 153 185 445 1498
Fragments arranged by size:
2505 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2784 4304 6016
Size: 274 80 33 51 1443 807 1520 1712
Cuts at: 6016 96
Size: 138
Fragments arranged by size:
1712 1520 1443 807 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2117 2120 2149 2152 6018 6050 2117
Size: 3 29 3 3866 32 2125
Fragments arranged by size:
3866 2125 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 1316 2345 2642 1316
Size: 1029 297 4732
Fragments arranged by size:
4732 1029 297
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 2670 1943
Size: 727 5331
XmaI C'CCGG_G
Cuts at: 1870 2013 3383 1870
Size: 143 1370 4545
Fragments arranged by size:
4545 1370 143
XmnI GAAnn'nnTTC
Cuts at: 4187 4187
Size: 6058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2104 3025 3112 3866
Size: 53 83 186 936 570 921 87 754
Cuts at: 3866 276
Size: 2468
Fragments arranged by size:
2468 936 921 754 570 186 87 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PspXI PsrI
PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI
XcmI