Restriction map for
pFN21AB6657
(Circular) (Six-base) MAPSORT of: pFN21AB6657.seq Check: 4037 from: 1 to: 5974
pFN21AB6657 5974 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2270 2654 3180 3317 1937
Size: 333 384 526 137 4594
Fragments arranged by size:
4594 526 384 333 137
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 2935 4101 4474 1563
Size: 1372 1166 373 3063
Fragments arranged by size:
3063 1372 1166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2829 4037 5421 5689 1632
Size: 1197 1208 1384 268 1917
Fragments arranged by size:
1917 1384 1208 1197 268
AflII C'TTAA_G
Cuts at: 820 1017 2873 820
Size: 197 1856 3921
Fragments arranged by size:
3921 1856 197
AflIII A'CryG_T
Cuts at: 1203 2152 4873 5850 1203
Size: 949 2721 977 1327
Fragments arranged by size:
2721 1327 977 949
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 2188 2807 3076 3717 5289 2188
Size: 619 269 641 1572 2873
Fragments arranged by size:
2873 1572 641 619 269
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2612 3283 3509 3661 5770 1493
Size: 1119 671 226 152 2109 1697
Fragments arranged by size:
2109 1697 1119 671 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 1870
Size: 73 1356 4545
Fragments arranged by size:
4545 1356 73
BamHI G'GATC_C
Cuts at: 2977 3304 3671 2977
Size: 327 367 5280
Fragments arranged by size:
5280 367 327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3293 1391
Size: 1902 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2726 3168 928
Size: 971 827 442 3734
Fragments arranged by size:
3734 971 827 442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3109 3866 5076 3109
Size: 757 1210 4007
Fragments arranged by size:
4007 1210 757
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BfrBI ATG'CAT
Cuts at: 2295 2295
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2646 5969 2646
Size: 3323 2651
BlpI GC'TnA_GC
Cuts at: 2010 3391 2010
Size: 1381 4593
Bme1580I G_kGCm'C
Cuts at: 1790 4035 5191 1790
Size: 2245 1156 2573
Fragments arranged by size:
2573 2245 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3705 4663 442
Size: 416 53 83 399 24 2288 958 1753
Fragments arranged by size:
2288 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4634 1239
Size: 3395 2579
Bpu10I CC'TnA_GC
Cuts at: 2602 2602
Size: 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4099 4964 5262 4099
Size: 865 298 4811
Fragments arranged by size:
4811 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3060 4637 882
Size: 617 1561 1577 2219
Fragments arranged by size:
2219 1577 1561 617
BsaAI yAC'GTr
Cuts at: 493 1204 2260 3765 493
Size: 711 1056 1505 2702
Fragments arranged by size:
2702 1505 1056 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2731 2761 1178
Size: 30 165 30 1328 30 4391
Fragments arranged by size:
4391 1328 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 3126 3228 1186
Size: 1516 424 102 3932
Fragments arranged by size:
3932 1516 424 102
BseYI C'CCAG_C
Cuts at: 2280 5177 5673 2280
Size: 2897 496 2581
Fragments arranged by size:
2897 2581 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2259 1163
Size: 573 523 4878
Fragments arranged by size:
4878 573 523
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4035 4120 5191 4035
Size: 85 1071 4818
Fragments arranged by size:
4818 1071 85
BsmI GAATG_Cn'
Cuts at: 2199 3485 3578 2199
Size: 1286 93 4595
Fragments arranged by size:
4595 1286 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3293 4035 4120 5191 1391
Size: 399 1503 742 85 1071 2174
Fragments arranged by size:
2174 1503 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3157 3863 5593 3157
Size: 706 1730 3538
Fragments arranged by size:
3538 1730 706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2297 4469 4643 65
Size: 1183 1049 2172 174 1396
Fragments arranged by size:
2172 1396 1183 1049 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2343 2376 2646 2977 3086 3304 3671 4056
Size: 371 33 270 331 109 218 367 385
Cuts at: 4056 4073 5525 5611 5623 5969 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 371 367 346 331 270
218 109 86 33 17 12
BstZ17I GTA'TAC
Cuts at: 2271 2271
Size: 5974
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2551 2626 2827 3490 4284 4304 2551
Size: 75 201 663 794 20 4221
Fragments arranged by size:
4221 794 663 201 75 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2767 3421 4310
Size: 54 1425 50 275 18 937 654 889
Cuts at: 4310 5939 8
Size: 1629 43
Fragments arranged by size:
1629 1425 937 889 654 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3904 1353
Size: 2551 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2829 4037 4634 5421 5689 1239
Size: 393 1197 1208 597 787 268 1524
Fragments arranged by size:
1524 1208 1197 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2174 2174
Size: 5974
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 2846 2878 4615
Size: 32 135 32 878 32 364 32 1737
Cuts at: 4615 4647 4689 4721 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1737 878 364 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2880 3318 3564 669
Size: 988 281 942 438 246 3079
Fragments arranged by size:
3079 988 942 438 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 2409 3573 2409
Size: 1164 4810
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2769 10
Size: 54 1425 343 937 3215
Fragments arranged by size:
3215 1425 937 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2151 2216 2424 3932 4291 4450 518
Size: 648 985 65 208 1508 359 159 2042
Fragments arranged by size:
2042 1508 985 648 359 208 159 65
MspA1I CmG'CkG
Cuts at: 1606 3076 3390 4067 5215 5460 1606
Size: 1470 314 677 1148 245 2120
Fragments arranged by size:
2120 1470 1148 677 314 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NsiI A_TGCA'T
Cuts at: 2297 2297
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 2156 2299 3332 4877 1132
Size: 97 927 143 1033 1545 2229
Fragments arranged by size:
2229 1545 1033 927 143 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 2152 4873 2152
Size: 2721 3253
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2046 2078 2046
Size: 32 5942
PstI C_TGCA'G
Cuts at: 830 2822 3326 830
Size: 1992 504 3478
Fragments arranged by size:
3478 1992 504
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 3076 3076
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 2557 2586 2655 2664 2818 3238 3322
Size: 219 1512 29 69 9 154 420 84
Cuts at: 3322 4457 5138 5329 826
Size: 1135 681 191 1471
Fragments arranged by size:
1512 1471 1135 681 420 219 191 154
84 69 29 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2873 4078 4979 5241 820
Size: 197 926 930 1205 901 262 1553
Fragments arranged by size:
1553 1205 930 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 2199 2385 3413 513
Size: 552 939 195 186 1028 3074
Fragments arranged by size:
3074 1028 939 552 195 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2437 3025 4001 4018 4171 4356 4801
Size: 1255 857 588 976 17 153 185 445
Cuts at: 4801 325
Size: 1498
Fragments arranged by size:
1498 1255 976 857 588 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4220 5932 96
Size: 274 80 33 51 1443 2243 1712 138
Fragments arranged by size:
2243 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2883 2915 5934 5966 2883
Size: 32 3019 32 2891
Fragments arranged by size:
3019 2891 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI