Restriction map for
pFN21AB6656
(Circular) (Six-base) MAPSORT of: pFN21AB6656.seq Check: 4961 from: 1 to: 6055
pFN21AB6656 6055 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3189 3865 278
Size: 53 83 186 936 1653 676 2468
Fragments arranged by size:
2468 1653 936 676 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 2225 3376 2225
Size: 1151 4904
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 2730 3200 4118 5502 5770 1632
Size: 977 121 470 918 1384 268 1917
Fragments arranged by size:
1917 1384 977 918 470 268 121
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 3108 4954 5931 1203
Size: 1905 1846 977 1327
Fragments arranged by size:
1905 1846 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 2117 1166
Size: 951 5104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2765 1184
Size: 1581 4474
AlwNI CAG_nnn'CTG
Cuts at: 2086 2565 2758 3068 3798 5370 2086
Size: 479 193 310 730 1572 2771
Fragments arranged by size:
2771 1572 730 479 310 193
ApaI G_GGCC'C
Cuts at: 2572 2572
Size: 6055
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 3089 3171 3364 3590 3742 5851 1493
Size: 1596 82 193 226 152 2109 1697
Fragments arranged by size:
2109 1697 1596 226 193 152 82
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 3195 3380 1870
Size: 73 1252 185 4545
Fragments arranged by size:
4545 1252 185 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2225 3273 3376 4831 618
Size: 325 217 627 438 1048 103 1455 1842
Fragments arranged by size:
1842 1455 1048 627 438 325 217 103
BanII G_rGCy'C
Cuts at: 1391 2572 2790 3374 1391
Size: 1181 218 584 4072
Fragments arranged by size:
4072 1181 584 218
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5084
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2672 2706 2914 2948 4246 4280 1903
Size: 34 735 34 208 34 1298 34 3678
Fragments arranged by size:
3678 1298 735 208 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2175 2705 3071
Size: 107 122 71 631 605 503 530 366
Cuts at: 3071 4666 136
Size: 1595 1525
Fragments arranged by size:
1595 1525 631 605 530 503 366 122
107 71
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 2280 2417 3472 2280
Size: 137 1055 4863
Fragments arranged by size:
4863 1055 137
Bme1580I G_kGCm'C
Cuts at: 1790 2531 2572 4116 5272 1790
Size: 741 41 1544 1156 2573
Fragments arranged by size:
2573 1544 1156 741 41
BmgBI CAC'GTC
Cuts at: 1318 2107 2629 3109 1318
Size: 789 522 480 4264
Fragments arranged by size:
4264 789 522 480
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3786 4744 442
Size: 416 53 83 399 24 2369 958 1753
Fragments arranged by size:
2369 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2126 2891 2126
Size: 765 5290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2939 4715 1239
Size: 1700 1776 2579
Fragments arranged by size:
2579 1776 1700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2864 3344 4180 5045 5343 2864
Size: 480 836 865 298 3576
Fragments arranged by size:
3576 865 836 480 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2937 3011 4718 882
Size: 617 1438 74 1707 2219
Fragments arranged by size:
2219 1707 1438 617 74
BsaAI yAC'GTr
Cuts at: 493 1204 3846 493
Size: 711 2642 2702
Fragments arranged by size:
2702 2642 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2685 3020 3186 3282
Size: 53 83 186 936 1152 335 166 96
Cuts at: 3282 3862 4244 4879 275
Size: 580 382 635 1451
Fragments arranged by size:
1451 1152 936 635 580 382 335 186
166 96 83 53
BsaWI w'CCGG_w
Cuts at: 2033 4488 4886 5160 5307 5718 2033
Size: 2455 398 274 147 411 2370
Fragments arranged by size:
2455 2370 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5830
Fragments arranged by size:
5830 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 2789 2867 1186
Size: 1258 345 78 4374
Fragments arranged by size:
4374 1258 345 78
BseYI C'CCAG_C
Cuts at: 2185 2565 2635 2860 5258 5754 2185
Size: 380 70 225 2398 496 2486
Fragments arranged by size:
2486 2398 496 380 225 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2771 3332 1163
Size: 573 1035 561 3886
Fragments arranged by size:
3886 1035 573 561
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2112 2333 2388 2469 3330 4116 4201 5272 2112
Size: 221 55 81 861 786 85 1071 2895
Fragments arranged by size:
2895 1071 861 786 221 85 81 55
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
BsmBI CGTCTCn'nnnn_
Cuts at: 2097 2619 2097
Size: 522 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2333 2388 2469 2531 2572 2790
Size: 399 322 221 55 81 62 41 218
Cuts at: 2790 3330 3374 4116 4201 5272 1391
Size: 540 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 540 399 322 221 218
85 81 62 55 44 41
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 2721 3258 3944 5674 2721
Size: 537 686 1730 3102
Fragments arranged by size:
3102 1730 686 537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 2844 3410 844
Size: 1250 750 566 3489
Fragments arranged by size:
3489 1250 750 566
BsrBI CCG'CTC
Cuts at: 1394 1823 2284 3942 1394
Size: 429 461 1658 3507
Fragments arranged by size:
3507 1658 461 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2965 4550 4724 65
Size: 1183 1717 1585 174 1396
Fragments arranged by size:
1717 1585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4699 4886 1700
Size: 110 2889 187 2869
Fragments arranged by size:
2889 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 3341 4115 5127 3341
Size: 774 1012 4269
Fragments arranged by size:
4269 1012 774
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2086 794
Size: 1292 4763
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstXI CCAn_nnnn'nTGG
Cuts at: 3057 3057
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 3385 3752 4137 4154 5606 5692 5704 6050
Size: 1413 367 385 17 1452 86 12 346
Cuts at: 6050 1972
Size: 1977
Fragments arranged by size:
1977 1452 1413 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3069 3069
Size: 6055
BtgI C'CryG_G
Cuts at: 513 1065 2065 2254 2907 513
Size: 552 1000 189 653 3661
Fragments arranged by size:
3661 1000 653 552 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 2070 2688 3571 4365 4385 2070
Size: 618 883 794 20 3740
Fragments arranged by size:
3740 883 794 618 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DraIII CAC_nnn'GTG
Cuts at: 2358 2358
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 2121 2811
Size: 54 1425 50 275 18 197 94 690
Cuts at: 2811 2985 3502 4391 6020 8
Size: 174 517 889 1629 43
Fragments arranged by size:
1629 1425 889 690 517 275 197 174
94 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3985 1353
Size: 2632 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2880 4642 5016 5162 387
Size: 1064 138 1291 1762 374 146 1280
Fragments arranged by size:
1762 1291 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 2730 2939 3200 4118 4715 5502
Size: 393 977 121 209 261 918 597 787
Cuts at: 5502 5770 1239
Size: 268 1524
Fragments arranged by size:
1524 977 918 787 597 393 268 261
209 121
EcoO109I rG'GnC_Cy
Cuts at: 2083 2134 2569 3073 2083
Size: 51 435 504 5065
Fragments arranged by size:
5065 504 435 51
EcoRI G'AATT_C
Cuts at: 3171 3364 3742 3171
Size: 193 378 5484
Fragments arranged by size:
5484 378 193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 6055
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 5202 1678
Size: 3524 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4696 4728 4770 4802 1373
Size: 32 135 32 3124 32 42 32 2626
Fragments arranged by size:
3124 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 2229 3380 2229
Size: 1151 4904
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5169 5353 1305
Size: 3864 184 2007
Fragments arranged by size:
3864 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2029 2813 2987 10
Size: 54 1425 343 197 784 174 3078
Fragments arranged by size:
3078 1425 784 343 197 174 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 4013 4372 4531 518
Size: 648 951 1896 359 159 2042
Fragments arranged by size:
2042 1896 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2458 2562 3471 4148 5296 5541 1606
Size: 852 104 909 677 1148 245 2120
Fragments arranged by size:
2120 1148 909 852 677 245 104
NcoI C'CATG_G
Cuts at: 513 1065 2907 513
Size: 552 1842 3661
Fragments arranged by size:
3661 1842 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 3413 4958 1132
Size: 97 2184 1545 2229
Fragments arranged by size:
2229 2184 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6055
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2134 2083
Size: 51 6004
PshAI GACnn'nnGTC
Cuts at: 1613 3003 1613
Size: 1390 4665
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PspOMI G'GGCC_C
Cuts at: 2568 2568
Size: 6055
PstI C_TGCA'G
Cuts at: 830 2601 3407 830
Size: 1771 806 3478
Fragments arranged by size:
3478 1771 806
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2458 2458
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SexAI A'CCwGG_T
Cuts at: 2745 3351 2745
Size: 606 5449
SfcI C'TryA_G
Cuts at: 826 1045 2597 3305 3403 4538 5219 5410 826
Size: 219 1552 708 98 1135 681 191 1471
Fragments arranged by size:
1552 1471 1135 708 681 219 191 98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2879 3323 4159 5060 5322 820
Size: 197 926 936 444 836 901 262 1553
Fragments arranged by size:
1553 936 926 901 836 444 262 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 2310 2699 1517
Size: 793 389 4873
Fragments arranged by size:
4873 793 389
StyI C'CwwG_G
Cuts at: 513 1065 2130 2246 2814 2907 2988 3012 3494
Size: 552 1065 116 568 93 81 24 482
Cuts at: 3494 513
Size: 3074
Fragments arranged by size:
3074 1065 568 552 482 116 93 81
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2841 3044 4082 4099 4252 4437 4882
Size: 1255 1261 203 1038 17 153 185 445
Cuts at: 4882 325
Size: 1498
Fragments arranged by size:
1498 1261 1255 1038 445 203 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2055 2436 2805
Size: 274 80 33 51 1443 78 381 369
Cuts at: 2805 4301 6013 96
Size: 1496 1712 138
Fragments arranged by size:
1712 1496 1443 381 369 274 138 80
78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6015 6047 6015
Size: 32 6023
Tth111I GACn'n_nGTC
Cuts at: 1316 3188 1316
Size: 1872 4183
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2161 2914 2161
Size: 753 5302
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 3089 4184 3089
Size: 1095 4960
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3187 3863 276
Size: 53 83 186 936 1653 676 2468
Fragments arranged by size:
2468 1653 936 676 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI