Restriction map for
pFN21AB6655
(Circular) (Six-base) MAPSORT of: pFN21AB6655.seq Check: 3764 from: 1 to: 5974
pFN21AB6655 5974 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2067 3784 278
Size: 53 83 186 936 531 1717 2468
Fragments arranged by size:
2468 1717 936 531 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 3216 4037 5421 5689 1632
Size: 653 931 821 1384 268 1917
Fragments arranged by size:
1917 1384 931 821 653 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 3006 4805 3006
Size: 1799 4175
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 2255 1166
Size: 1089 4885
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2741 2773 3023 3055 2741
Size: 32 250 32 5660
Fragments arranged by size:
5660 250 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3717 5289 3717
Size: 1572 4402
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2689 3283 3509 3661 5770 1493
Size: 1196 594 226 152 2109 1697
Fragments arranged by size:
2109 1697 1196 594 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 1870
Size: 73 1356 4545
Fragments arranged by size:
4545 1356 73
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3293 1391
Size: 1902 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3169 3866 5076 3169
Size: 697 1210 4067
Fragments arranged by size:
4067 1210 697
BclI T'GATC_A
Cuts at: 1548 2727 2835 1548
Size: 1179 108 4687
Fragments arranged by size:
4687 1179 108
BfrBI ATG'CAT
Cuts at: 2552 2568 2552
Size: 16 5958
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3131 4585 136
Size: 107 122 71 631 605 1459 1454 1525
Fragments arranged by size:
1525 1459 1454 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2877 5969 2877
Size: 3092 2882
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 4035 5191 1790
Size: 2245 1156 2573
Fragments arranged by size:
2573 2245 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2069 3705 4663
Size: 416 53 83 399 24 652 1636 958
Cuts at: 4663 442
Size: 1753
Fragments arranged by size:
1753 1636 958 652 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2401 2065
Size: 336 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 3050 3209 3275 4634 1239
Size: 821 990 159 66 1359 2579
Fragments arranged by size:
2579 1359 990 821 159 66
Bpu10I CC'TnA_GC
Cuts at: 2103 2103
Size: 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4099 4964 5262 4099
Size: 865 298 4811
Fragments arranged by size:
4811 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2213 2600 3065 3074 4637 882
Size: 617 714 387 465 9 1563 2219
Fragments arranged by size:
2219 1563 714 617 465 387 9
BsaAI yAC'GTr
Cuts at: 493 1204 2173 3765 493
Size: 711 969 1592 2702
Fragments arranged by size:
2702 1592 969 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 3781 4163 4798
Size: 53 83 186 936 531 1717 382 635
Cuts at: 4798 275
Size: 1451
Fragments arranged by size:
1717 1451 936 635 531 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3006 4407 4805 5079 5226 5637 3006
Size: 1401 398 274 147 411 3343
Fragments arranged by size:
3343 1401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2741 2771 3023 3053 1178
Size: 30 165 30 1338 30 252 30 4099
Fragments arranged by size:
4099 1338 252 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5974
BseYI C'CCAG_C
Cuts at: 2889 5177 5673 2889
Size: 2288 496 3190
Fragments arranged by size:
3190 2288 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4035 4120 5191 4035
Size: 85 1071 4818
Fragments arranged by size:
4818 1071 85
BsmI GAATG_Cn'
Cuts at: 2552 3485 3578 2552
Size: 933 93 4948
Fragments arranged by size:
4948 933 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2428 2428
Size: 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3293 4035 4120 5191 1391
Size: 399 1503 742 85 1071 2174
Fragments arranged by size:
2174 1503 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 3329 844
Size: 1286 1199 3489
Fragments arranged by size:
3489 1286 1199
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2200 4469 4643 65
Size: 1183 952 2269 174 1396
Fragments arranged by size:
2269 1396 1183 952 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3006 4618 4805 1700
Size: 110 1196 1612 187 2869
Fragments arranged by size:
2869 1612 1196 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 3002 1184
Size: 1818 4156
BstXI CCAn_nnnn'nTGG
Cuts at: 2257 3112 2257
Size: 855 5119
BstYI r'GATC_y
Cuts at: 1972 2877 2991 3304 3671 4056 4073 5525 5611
Size: 905 114 313 367 385 17 1452 86
Cuts at: 5611 5623 5969 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 905 385 367 346 313 114
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2684 2684
Size: 5974
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 513
Size: 552 939 48 4435
Fragments arranged by size:
4435 939 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2637 3490 4284 4304 2637
Size: 853 794 20 4307
Fragments arranged by size:
4307 853 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DraIII CAC_nnn'GTG
Cuts at: 2701 2701
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2079 3114
Size: 54 1425 50 275 18 177 72 1035
Cuts at: 3114 3421 4310 5939 8
Size: 307 889 1629 43
Fragments arranged by size:
1629 1425 1035 889 307 275 177 72
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2591 3192 3213 3904 1353
Size: 1238 601 21 691 3423
Fragments arranged by size:
3423 1238 691 601 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2285 3050 3209 3216 3275 4037
Size: 393 428 225 765 159 7 59 762
Cuts at: 4037 4634 5421 5689 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 765 762 597 428 393 268
225 159 59 7
EcoO109I rG'GnC_Cy
Cuts at: 2380 2680 2380
Size: 300 5674
EcoRI G'AATT_C
Cuts at: 2689 3283 3661 2689
Size: 594 378 5002
Fragments arranged by size:
5002 594 378
EcoRV GAT'ATC
Cuts at: 2242 2782 2242
Size: 540 5434
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3248 3280 4615 4647 4689
Size: 32 135 32 1676 32 1335 32 42
Cuts at: 4689 4721 1373
Size: 32 2626
Fragments arranged by size:
2626 1676 1335 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3052 3318 3564 669
Size: 988 281 1114 266 246 3079
Fragments arranged by size:
3079 1114 988 281 266 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2081 10
Size: 54 1425 343 177 72 3903
Fragments arranged by size:
3903 1425 343 177 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2951 3932 4291 4450 518
Size: 648 1089 696 981 359 159 2042
Fragments arranged by size:
2042 1089 981 696 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2833 2893 3390 4067 5215 5460 1606
Size: 1227 60 497 677 1148 245 2120
Fragments arranged by size:
2120 1227 1148 677 497 245 60
NcoI C'CATG_G
Cuts at: 513 1065 2004 2052 513
Size: 552 939 48 4435
Fragments arranged by size:
4435 939 552 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NsiI A_TGCA'T
Cuts at: 2554 2570 2554
Size: 16 5958
NspI r_CATG'y
Cuts at: 1132 1229 2572 2961 3182 3332 4877 1132
Size: 97 1343 389 221 150 1545 2229
Fragments arranged by size:
2229 1545 1343 389 221 150 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2701 1108
Size: 1593 4381
PfoI T'CCnGG_A
Cuts at: 2037 2387 2037
Size: 350 5624
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2773 3023 3055 4043 4075 5556 5588 2741
Size: 32 250 32 988 32 1481 32 3127
Fragments arranged by size:
3127 1481 988 250 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2380 2680 2380
Size: 300 5674
PshAI GACnn'nnGTC
Cuts at: 1613 2033 2612 1613
Size: 420 579 4975
Fragments arranged by size:
4975 579 420
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PstI C_TGCA'G
Cuts at: 830 3326 830
Size: 2496 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2893 2893
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SapI GCTCTTCn'nnn_
Cuts at: 3213 3213
Size: 5974
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 3322 4457 5138 5329 826
Size: 219 2277 1135 681 191 1471
Fragments arranged by size:
2277 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4078 4979 5241 820
Size: 197 926 2135 901 262 1553
Fragments arranged by size:
2135 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 3413 513
Size: 552 939 48 1361 3074
Fragments arranged by size:
3074 1361 939 552 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 2639 4001 4018 4171 4356 4801
Size: 1255 672 387 1362 17 153 185 445
Cuts at: 4801 325
Size: 1498
Fragments arranged by size:
1498 1362 1255 672 445 387 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4220 5932 96
Size: 274 80 33 51 1443 2243 1712 138
Fragments arranged by size:
2243 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 2383 2415 5934 5966 2252
Size: 32 99 32 3519 32 2260
Fragments arranged by size:
3519 2260 99 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2905 2905
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2065 3782 276
Size: 53 83 186 936 531 1717 2468
Fragments arranged by size:
2468 1717 936 531 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI