Restriction map for
pFN21AB6647
(Circular) (Six-base) MAPSORT of: pFN21AB6647.seq Check: 5038 from: 1 to: 5917
pFN21AB6647 5917 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2355 3260 1937
Size: 418 905 4594
Fragments arranged by size:
4594 905 418
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2132 2617 3980 5364 5632 1632
Size: 500 485 1363 1384 268 1917
Fragments arranged by size:
1917 1384 1363 500 485 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2432 4816 5793 1203
Size: 1229 2384 977 1327
Fragments arranged by size:
2384 1327 1229 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AlwNI CAG_nnn'CTG
Cuts at: 2149 2999 3660 5232 2149
Size: 850 661 1572 2834
Fragments arranged by size:
2834 1572 850 661
ApaI G_GGCC'C
Cuts at: 2128 2934 2128
Size: 806 5111
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 3226 3452 3604 5713 1493
Size: 1733 226 152 2109 1697
Fragments arranged by size:
2109 1733 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2595 3242 1870
Size: 73 652 647 4545
Fragments arranged by size:
4545 652 647 73
BamHI G'GATC_C
Cuts at: 2274 3042 3247 3614 2274
Size: 768 205 367 4577
Fragments arranged by size:
4577 768 367 205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2298 2497 3238 4693 618
Size: 325 217 627 511 199 741 1455 1842
Fragments arranged by size:
1842 1455 741 627 511 325 217 199
BanII G_rGCy'C
Cuts at: 1391 2128 2559 2934 3236 1391
Size: 737 431 375 302 4072
Fragments arranged by size:
4072 737 431 375 302
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4528 136
Size: 107 122 71 631 605 2856 1525
Fragments arranged by size:
2856 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5912 5912
Size: 5917
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 2128 2835 2934 3978 5134 1790
Size: 338 707 99 1044 1156 2573
Fragments arranged by size:
2573 1156 1044 707 338 99
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4606 442
Size: 416 53 83 399 24 2231 958 1753
Fragments arranged by size:
2231 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2729 3228 4577 1239
Size: 1490 499 1349 2579
Fragments arranged by size:
2579 1490 1349 499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4042 4907 5205 4042
Size: 865 298 4754
Fragments arranged by size:
4754 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2321 4580 882
Size: 617 822 2259 2219
Fragments arranged by size:
2259 2219 822 617
BsaAI yAC'GTr
Cuts at: 493 1204 3708 493
Size: 711 2504 2702
Fragments arranged by size:
2702 2504 711
BsaBI GATnn'nnATC
Cuts at: 5911 5911
Size: 5917
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3724 4106 4741 275
Size: 53 83 186 936 2191 382 635 1451
Fragments arranged by size:
2191 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2295 4350 4748 5022 5169 5580 2295
Size: 2055 398 274 147 411 2632
Fragments arranged by size:
2632 2055 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2019 2049 2318 2348 2505
Size: 30 165 30 616 30 269 30 157
Cuts at: 2505 2535 3201 3231 1178
Size: 30 666 30 3864
Fragments arranged by size:
3864 666 616 269 165 157 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2223 2616 2726 2793 2993 3126 3131 3164
Size: 1037 393 110 67 200 133 5 33
Cuts at: 3164 3167 1186
Size: 3 3936
Fragments arranged by size:
3936 1037 393 200 133 110 67 33
5 3
BseYI C'CCAG_C
Cuts at: 2142 2590 2793 2849 3016 5120 5616 2142
Size: 448 203 56 167 2104 496 2443
Fragments arranged by size:
2443 2104 496 448 203 167 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3180 1163
Size: 573 1444 3900
Fragments arranged by size:
3900 1444 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 4128 5156 1540
Size: 275 186 1366 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2074 3978 4063 5134 2074
Size: 1904 85 1071 2857
Fragments arranged by size:
2857 1904 1071 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2128 2559 2835 2934 3236 3978
Size: 399 284 54 431 276 99 302 742
Cuts at: 3978 4063 5134 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 431 399 302 284 276
99 85 54
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3272 844
Size: 2428 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2313 4412 4586 65
Size: 1183 1065 2099 174 1396
Fragments arranged by size:
2099 1396 1183 1065 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4561 4748 1700
Size: 110 2751 187 2869
Fragments arranged by size:
2869 2751 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 2232 3977 4989 2232
Size: 1745 1012 3160
Fragments arranged by size:
3160 1745 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 2584 1184
Size: 1400 4517
BstXI CCAn_nnnn'nTGG
Cuts at: 2366 2425 2366
Size: 59 5858
BstYI r'GATC_y
Cuts at: 1972 2274 2605 3042 3247 3614 3999 4016 5468
Size: 302 331 437 205 367 385 17 1452
Cuts at: 5468 5554 5566 5912 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 437 385 367 346 331 302
205 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2883 3186 2883
Size: 303 5614
BtgI C'CryG_G
Cuts at: 513 1065 2004 2926 513
Size: 552 939 922 3504
Fragments arranged by size:
3504 939 922 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 2564 3433 4227 4247 2564
Size: 869 794 20 4234
Fragments arranged by size:
4234 869 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2437 4924 809
Size: 507 1121 2487 1802
Fragments arranged by size:
2487 1802 1121 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2910 3364 4253
Size: 54 1425 50 275 18 1080 454 889
Cuts at: 4253 5882 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1080 889 454 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 2564 3141 3847 1353
Size: 908 303 577 706 3423
Fragments arranged by size:
3423 908 706 577 303
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2617 2729 3228 3980 4577 5364
Size: 393 500 485 112 499 752 597 787
Cuts at: 5364 5632 1239
Size: 268 1524
Fragments arranged by size:
1524 787 752 597 500 499 485 393
268 112
EcoNI CCTnn'n_nnAGG
Cuts at: 2550 2874 3189 2550
Size: 324 315 5278
Fragments arranged by size:
5278 324 315
EcoO109I rG'GnC_Cy
Cuts at: 2124 2125 2191 2213 2879 2931 3127 3212 2124
Size: 1 66 22 666 52 196 85 4829
Fragments arranged by size:
4829 666 196 85 66 52 22 1
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FspI TGC'GCA
Cuts at: 2666 4423 2666
Size: 1757 4160
HaeII r_GCGC'y
Cuts at: 1678 5064 1678
Size: 3386 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2337 2350 2369 2378
Size: 32 135 32 746 19 13 19 9
Cuts at: 2378 2410 2866 2898 4558 4590 4632 4664 1373
Size: 32 456 32 1660 32 42 32 2626
Fragments arranged by size:
2626 1660 746 456 135 42 32 32
32 32 32 32 19 19 13 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 3507 669
Size: 988 281 1323 246 3079
Fragments arranged by size:
3079 1323 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2181 748
Size: 1433 4484
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 2720 3516 2720
Size: 796 5121
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2005 2361 2553 3156 5031 5215 1305
Size: 700 356 192 603 1875 184 2007
Fragments arranged by size:
2007 1875 700 603 356 192 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2912 10
Size: 54 1425 343 1080 3015
Fragments arranged by size:
3015 1425 1080 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2481 3875 4234 4393 518
Size: 648 1315 1394 359 159 2042
Fragments arranged by size:
2042 1394 1315 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2904 3333 4010 5158 5403 1606
Size: 540 758 429 677 1148 245 2120
Fragments arranged by size:
2120 1148 758 677 540 429 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4426
Fragments arranged by size:
4426 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 2436 3275 4820 1132
Size: 97 1207 839 1545 2229
Fragments arranged by size:
2229 1545 1207 839 97
PasI CC'CwG_GG
Cuts at: 1348 2203 2217 3037 1348
Size: 855 14 820 4228
Fragments arranged by size:
4228 855 820 14
PciI A'CATG_T
Cuts at: 2432 4816 2432
Size: 2384 3533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2149 2725 2741 1108
Size: 1041 576 16 4284
Fragments arranged by size:
4284 1041 576 16
PfoI T'CCnGG_A
Cuts at: 2277 2277
Size: 5917
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2191
Size: 5917
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PspOMI G'GGCC_C
Cuts at: 2124 2930 2124
Size: 806 5111
PstI C_TGCA'G
Cuts at: 830 2461 2571 2995 3269 830
Size: 1631 110 424 274 3478
Fragments arranged by size:
3478 1631 424 274 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
PvuII CAG'CTG
Cuts at: 2904 2904
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SapI GCTCTTCn'nnn_
Cuts at: 2564 2564
Size: 5917
SbfI CC_TGCA'GG
Cuts at: 2461 3269 2461
Size: 808 5109
ScaI AGT'ACT
Cuts at: 3205 4165 5877 3205
Size: 960 1712 3245
Fragments arranged by size:
3245 1712 960
SfcI C'TryA_G
Cuts at: 826 1045 2457 2567 2626 2899 2991 3265 4400
Size: 219 1412 110 59 273 92 274 1135
Cuts at: 4400 5081 5272 826
Size: 681 191 1471
Fragments arranged by size:
1471 1412 1135 681 274 273 219 191
110 92 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 2597 3244 1872
Size: 725 647 4545
Fragments arranged by size:
4545 725 647
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4021 4922 5184 820
Size: 197 926 2078 901 262 1553
Fragments arranged by size:
2078 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 2643 3054 1517
Size: 1126 411 4380
Fragments arranged by size:
4380 1126 411
StyI C'CwwG_G
Cuts at: 513 1065 2004 3356 513
Size: 552 939 1352 3074
Fragments arranged by size:
3074 1352 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 2930 3944 3961 4114 4299 4744
Size: 1255 443 907 1014 17 153 185 445
Cuts at: 4744 325
Size: 1498
Fragments arranged by size:
1498 1255 1014 907 445 443 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3203 4163 5875
Size: 274 80 33 51 1443 1226 960 1712
Cuts at: 5875 96
Size: 138
Fragments arranged by size:
1712 1443 1226 960 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 2505 2537 5877 5909 2019
Size: 32 454 32 3340 32 2027
Fragments arranged by size:
3340 2027 454 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2332 1316
Size: 1016 4901
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 2595 3242 1870
Size: 725 647 4545
Fragments arranged by size:
4545 725 647
XmnI GAAnn'nnTTC
Cuts at: 4046 4046
Size: 5917
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI