Restriction map for
pFN21AB6645
(Circular) (Six-base) MAPSORT of: pFN21AB6645.seq Check: 1061 from: 1 to: 5962
pFN21AB6645 5962 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2246 2246
Size: 5962
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2165 3010 4025 5409 5677 1632
Size: 533 845 1015 1384 268 1917
Fragments arranged by size:
1917 1384 1015 845 533 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 2130 4861 5838 1203
Size: 927 2731 977 1327
Fragments arranged by size:
2731 1327 977 927
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2852 3173 1184
Size: 1668 321 3973
Fragments arranged by size:
3973 1668 321
AlwNI CAG_nnn'CTG
Cuts at: 3705 5277 3705
Size: 1572 4390
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 3287 1870
Size: 73 1344 4545
Fragments arranged by size:
4545 1344 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2522 2638 2731 3283 4738
Size: 325 217 627 735 116 93 552 1455
Cuts at: 4738 618
Size: 1842
Fragments arranged by size:
1842 1455 735 627 552 325 217 116
93
BanII G_rGCy'C
Cuts at: 1391 2226 3281 1391
Size: 835 1055 4072
Fragments arranged by size:
4072 1055 835
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2539 2603 2984 3002 928
Size: 971 640 64 381 18 3888
Fragments arranged by size:
3888 971 640 381 64 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3109 3143 4153 4187 1903
Size: 34 1172 34 1010 34 3678
Fragments arranged by size:
3678 1172 1010 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3123 5957 3123
Size: 2834 3128
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 2197 2850 3152 4023 5179 1790
Size: 407 653 302 871 1156 2573
Fragments arranged by size:
2573 1156 871 653 407 302
BmgBI CAC'GTC
Cuts at: 1318 2908 1318
Size: 1590 4372
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3199 3693 4651
Size: 416 53 83 399 24 1782 494 958
Cuts at: 4651 442
Size: 1753
Fragments arranged by size:
1782 1753 958 494 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2900 4622 1239
Size: 1661 1722 2579
Fragments arranged by size:
2579 1722 1661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2879 2951 3203 4087 4952 5250 2879
Size: 72 252 884 865 298 3591
Fragments arranged by size:
3591 884 865 298 252 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2573 2772 4625 882
Size: 617 1074 199 1853 2219
Fragments arranged by size:
2219 1853 1074 617 199
BsaAI yAC'GTr
Cuts at: 493 1204 2932 3753 493
Size: 711 1728 821 2702
Fragments arranged by size:
2702 1728 821 711
BsaBI GATnn'nnATC
Cuts at: 2678 5956 2678
Size: 3278 2684
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 2684 2714 1178
Size: 30 165 30 714 30 537 30 4426
Fragments arranged by size:
4426 714 537 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 2417 2783 2816 3242 3245 1186
Size: 1228 3 366 33 426 3 3903
Fragments arranged by size:
3903 1228 426 366 33 3 3
BseYI C'CCAG_C
Cuts at: 2111 2219 2815 3097 5165 5661 2111
Size: 108 596 282 2068 496 2412
Fragments arranged by size:
2412 2068 596 496 282 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2812 1163
Size: 573 1076 4313
Fragments arranged by size:
4313 1076 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2856 3412 4173 5201 1540
Size: 275 186 855 556 761 1028 2301
Fragments arranged by size:
2301 1028 855 761 556 275 186
BsiHKAI G_wGCw'C
Cuts at: 4023 4108 5179 4023
Size: 85 1071 4806
Fragments arranged by size:
4806 1071 85
BsiWI C'GTAC_G
Cuts at: 2928 2928
Size: 5962
BsmI GAATG_Cn'
Cuts at: 2320 3473 3566 2320
Size: 1153 93 4716
Fragments arranged by size:
4716 1153 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2197 2226 2850 3152 3281 4023 4108
Size: 399 407 29 624 302 129 742 85
Cuts at: 4108 5179 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 624 407 399 302 129
85 29
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2246 2790 3317 844
Size: 1402 544 527 3489
Fragments arranged by size:
3489 1402 544 527
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2205 3145 4457 4631 65
Size: 1183 957 940 1312 174 1396
Fragments arranged by size:
1396 1312 1183 957 940 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4606 4793 1700
Size: 110 2796 187 2869
Fragments arranged by size:
2869 2796 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2262 794
Size: 1468 4494
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 3073 1184
Size: 1889 4073
BstXI CCAn_nnnn'nTGG
Cuts at: 2212 2654 2212
Size: 442 5520
BstYI r'GATC_y
Cuts at: 1972 2937 3036 3123 3292 3659 4044 4061 5513
Size: 965 99 87 169 367 385 17 1452
Cuts at: 5513 5599 5611 5957 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 965 385 367 346 169 99
87 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3225 3225
Size: 5962
BtgI C'CryG_G
Cuts at: 513 1065 2082 2850 3144 513
Size: 552 1017 768 294 3331
Fragments arranged by size:
3331 1017 768 552 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3184 487
Size: 683 56 1958 3265
Fragments arranged by size:
3265 1958 683 56
BtsI GCAGTG_nn'
Cuts at: 3478 4272 4292 3478
Size: 794 20 5148
Fragments arranged by size:
5148 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2537 4969 809
Size: 507 1221 2432 1802
Fragments arranged by size:
2432 1802 1221 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2676 2755 2853
Size: 54 1425 50 275 18 846 79 98
Cuts at: 2853 3180 3409 4298 5927 8
Size: 327 229 889 1629 43
Fragments arranged by size:
1629 1425 889 846 327 275 229 98
79 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2853 3409 1537
Size: 275 1041 556 4090
Fragments arranged by size:
4090 1041 556 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3892 1353
Size: 2539 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2900 3010 4025 4622 5409 5677
Size: 393 533 735 110 1015 597 787 268
Cuts at: 5677 1239
Size: 1524
Fragments arranged by size:
1524 1015 787 735 597 533 393 268
110
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 2378
Size: 5962
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 5109 1678
Size: 3431 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2684 2716 4603 4635 4677
Size: 32 135 32 1112 32 1887 32 42
Cuts at: 4677 4709 1373
Size: 32 2626
Fragments arranged by size:
2626 1887 1112 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3195 5076 5260 1305
Size: 1890 1881 184 2007
Fragments arranged by size:
2007 1890 1881 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2678 3182 10
Size: 54 1425 343 846 504 2790
Fragments arranged by size:
2790 1425 846 504 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2324 3920 4279 4438 518
Size: 648 1044 114 1596 359 159 2042
Fragments arranged by size:
2042 1596 1044 648 359 159 114
MspA1I CmG'CkG
Cuts at: 1606 3378 4055 5203 5448 1606
Size: 1772 677 1148 245 2120
Fragments arranged by size:
2120 1772 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5410
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2134 3178 3320 4865 1132
Size: 97 905 1044 142 1545 2229
Fragments arranged by size:
2229 1545 1044 905 142 97
PasI CC'CwG_GG
Cuts at: 1348 2190 1348
Size: 842 5120
PciI A'CATG_T
Cuts at: 2130 4861 2130
Size: 2731 3231
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3070 1108
Size: 1962 4000
PfoI T'CCnGG_A
Cuts at: 2877 2877
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2585 1613
Size: 972 4990
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2436 2490 3314 830
Size: 1606 54 824 3478
Fragments arranged by size:
3478 1606 824 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2432 2486 3310 4445 5126 5317 826
Size: 219 1387 54 824 1135 681 191 1471
Fragments arranged by size:
1471 1387 1135 824 681 219 191 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2894 2966 3218 4066 4967 5229
Size: 197 926 951 72 252 848 901 262
Cuts at: 5229 820
Size: 1553
Fragments arranged by size:
1553 951 926 901 848 262 252 197
72
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 2373 1517
Size: 856 5106
StyI C'CwwG_G
Cuts at: 513 1065 3401 513
Size: 552 2336 3074
Fragments arranged by size:
3074 2336 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4208 5920 96
Size: 274 80 33 51 1443 2231 1712 138
Fragments arranged by size:
2231 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2080 2080
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 2015 2156 4091 2015
Size: 141 1935 3886
Fragments arranged by size:
3886 1935 141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsmBI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI