Restriction map for
pFN21AB6644
(Circular) (Six-base) MAPSORT of: pFN21AB6644.seq Check: 5737 from: 1 to: 6034
pFN21AB6644 6034 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3844 278
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3377 1937
Size: 1440 4594
Acc65I G'GTAC_C
Cuts at: 3355 3355
Size: 6034
AclI AA'CG_TT
Cuts at: 1563 4161 4534 1563
Size: 2598 373 3063
Fragments arranged by size:
3063 2598 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3293 4097 5481 5749 1632
Size: 1661 804 1384 268 1917
Fragments arranged by size:
1917 1661 1384 804 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5837
AflIII A'CryG_T
Cuts at: 1203 4933 5910 1203
Size: 3730 977 1327
Fragments arranged by size:
3730 1327 977
AgeI A'CCGG_T
Cuts at: 2130 4865 2130
Size: 2735 3299
AhdI GACnn_n'nnGTC
Cuts at: 4763 4763
Size: 6034
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6034
AlwNI CAG_nnn'CTG
Cuts at: 2377 3777 5349 2377
Size: 1400 1572 3062
Fragments arranged by size:
3062 1572 1400
ApaLI G'TGCA_C
Cuts at: 4091 5247 4091
Size: 1156 4878
ApoI r'AATT_y
Cuts at: 1493 2762 3343 3569 3721 5830 1493
Size: 1269 581 226 152 2109 1697
Fragments arranged by size:
2109 1697 1269 581 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4588 160
Size: 872 3556 1606
Fragments arranged by size:
3556 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 2169 2931 2994 3359 1870
Size: 73 193 33 762 63 365 4545
Fragments arranged by size:
4545 762 365 193 73 63 33
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2943 2976 2943
Size: 33 6001
BamHI G'GATC_C
Cuts at: 2153 3364 3731 2153
Size: 1211 367 4456
Fragments arranged by size:
4456 1211 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2165 2860 2981 3355
Size: 325 217 627 291 87 695 121 374
Cuts at: 3355 4810 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 695 627 374 325 291 217
121 87
BanII G_rGCy'C
Cuts at: 1391 2609 3353 1391
Size: 1218 744 4072
Fragments arranged by size:
4072 1218 744
BbeI G_GCGC'C
Cuts at: 2169 2169
Size: 6034
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 2683 928
Size: 971 138 646 4279
Fragments arranged by size:
4279 971 646 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2242 2276 4225 4259 1903
Size: 34 50 34 221 34 1949 34 3678
Fragments arranged by size:
3678 1949 221 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2514 3060 3926 5136 2514
Size: 546 866 1210 3412
Fragments arranged by size:
3412 1210 866 546
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6034
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 2110 4645
Size: 107 122 71 631 605 386 52 2535
Cuts at: 4645 136
Size: 1525
Fragments arranged by size:
2535 1525 631 605 386 122 107 71
52
BglII A'GATC_T
Cuts at: 2586 6029 2586
Size: 3443 2591
BlpI GC'TnA_GC
Cuts at: 3451 3451
Size: 6034
Bme1580I G_kGCm'C
Cuts at: 1790 2083 4095 5251 1790
Size: 293 2012 1156 2573
Fragments arranged by size:
2573 2012 1156 293
BmgBI CAC'GTC
Cuts at: 1318 2855 1318
Size: 1537 4497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3765 4723 442
Size: 416 53 83 399 24 2348 958 1753
Fragments arranged by size:
2348 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6034
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 3045 2356
Size: 689 5345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3015 4694 1239
Size: 1776 1679 2579
Fragments arranged by size:
2579 1776 1679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4159 5024 5322 4159
Size: 865 298 4871
Fragments arranged by size:
4871 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2646 4697 882
Size: 617 1147 2051 2219
Fragments arranged by size:
2219 2051 1147 617
BsaAI yAC'GTr
Cuts at: 493 1204 2948 3825 493
Size: 711 1744 877 2702
Fragments arranged by size:
2702 1744 877 711
BsaBI GATnn'nnATC
Cuts at: 6028 6028
Size: 6034
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 2166 3841 4223
Size: 53 83 186 936 569 64 1675 382
Cuts at: 4223 4858 275
Size: 635 1451
Fragments arranged by size:
1675 1451 936 635 569 382 186 83
64 53
BsaWI w'CCGG_w
Cuts at: 2075 2130 2773 4467 4865 5139 5286 5697 2075
Size: 55 643 1694 398 274 147 411 2412
Fragments arranged by size:
2412 1694 643 411 398 274 147 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5809
Fragments arranged by size:
5809 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 2880 1186
Size: 1258 436 4340
Fragments arranged by size:
4340 1258 436
BseYI C'CCAG_C
Cuts at: 5237 5733 5237
Size: 496 5538
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2868 3072 1163
Size: 573 1132 204 4125
Fragments arranged by size:
4125 1132 573 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3484 4245 5273 1540
Size: 275 186 1483 761 1028 2301
Fragments arranged by size:
2301 1483 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3031 3046 3318 4095 4180 5251 3031
Size: 15 272 777 85 1071 3814
Fragments arranged by size:
3814 1071 777 272 85 15
BsmI GAATG_Cn'
Cuts at: 3545 3638 3545
Size: 93 5941
BsmBI CGTCTCn'nnnn_
Cuts at: 2112 2112
Size: 6034
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2609 3031 3046 3318 3353 4095
Size: 399 293 526 422 15 272 35 742
Cuts at: 4095 4180 5251 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 422 399 293 272
85 35 15
BspEI T'CCGG_A
Cuts at: 5697 5697
Size: 6034
BspHI T'CATG_A
Cuts at: 3923 5653 3923
Size: 1730 4304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2322 3389 844
Size: 1478 1067 3489
Fragments arranged by size:
3489 1478 1067
BsrBI CCG'CTC
Cuts at: 1394 1823 3921 1394
Size: 429 2098 3507
Fragments arranged by size:
3507 2098 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4529 4703 65
Size: 1183 3281 174 1396
Fragments arranged by size:
3281 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 2130 4678 4865 1700
Size: 110 230 90 2548 187 2869
Fragments arranged by size:
2869 2548 230 187 110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6034
BssHII G'CGCG_C
Cuts at: 1925 2030 1925
Size: 105 5929
BssSI C'ACGA_G
Cuts at: 2847 4094 5106 2847
Size: 1247 1012 3775
Fragments arranged by size:
3775 1247 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6034
BstBI TT'CG_AA
Cuts at: 4838 4838
Size: 6034
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6034
BstYI r'GATC_y
Cuts at: 1972 2153 2586 2680 3364 3731 4116 4133 5585
Size: 181 433 94 684 367 385 17 1452
Cuts at: 5585 5671 5683 6029 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 684 433 385 367 346 181
94 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2064 2158 2217 2745 2914 513
Size: 552 999 94 59 528 169 3633
Fragments arranged by size:
3633 999 552 528 169 94 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5295
Fragments arranged by size:
5295 683 56
BtsI GCAGTG_nn'
Cuts at: 3550 4344 4364 3550
Size: 794 20 5220
Fragments arranged by size:
5220 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5999
DraI TTT'AAA
Cuts at: 3338 3338
Size: 6034
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3265 5041 809
Size: 507 1949 1776 1802
Fragments arranged by size:
1949 1802 1776 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2274 2328
Size: 54 1425 50 275 18 384 60 54
Cuts at: 2328 3238 3481 4370 5999 8
Size: 910 243 889 1629 43
Fragments arranged by size:
1629 1425 910 889 384 275 243 60
54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3481 1537
Size: 275 1669 4090
Fragments arranged by size:
4090 1669 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2565 2628 3964 1353
Size: 1212 63 1336 3423
Fragments arranged by size:
3423 1336 1212 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2160 4621 4995 5141 387
Size: 1064 138 571 2461 374 146 1280
Fragments arranged by size:
2461 1280 1064 571 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3015 3293 4097 4694 5481 5749 1239
Size: 393 1383 278 804 597 787 268 1524
Fragments arranged by size:
1524 1383 804 787 597 393 278 268
EcoO109I rG'GnC_Cy
Cuts at: 2222 3277 2222
Size: 1055 4979
EcoRI G'AATT_C
Cuts at: 3343 3721 3343
Size: 378 5656
EcoRV GAT'ATC
Cuts at: 2791 2791
Size: 6034
FspI TGC'GCA
Cuts at: 2642 4540 2642
Size: 1898 4136
HaeII r_GCGC'y
Cuts at: 1678 2014 2169 2252 5181 1678
Size: 336 155 83 2929 2531
Fragments arranged by size:
2929 2531 336 155 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2124 4675 4707 4749
Size: 32 135 32 520 32 2551 32 42
Cuts at: 4749 4781 1373
Size: 32 2626
Fragments arranged by size:
2626 2551 520 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3378 3624 669
Size: 988 281 1440 246 3079
Fragments arranged by size:
3079 1440 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3231 748
Size: 2483 3551
HpaI GTT'AAC
Cuts at: 3624 3624
Size: 6034
KasI G'GCGC_C
Cuts at: 2165 2165
Size: 6034
KpnI G_GTAC'C
Cuts at: 3359 3359
Size: 6034
MfeI C'AATT_G
Cuts at: 3633 3633
Size: 6034
MluI A'CGCG_T
Cuts at: 5910 5910
Size: 6034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2902 5148 5332 1305
Size: 1597 2246 184 2007
Fragments arranged by size:
2246 2007 1597 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3240 10
Size: 54 1425 343 1408 2804
Fragments arranged by size:
2804 1425 1408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3321 3992 4351 4510 518
Size: 648 2155 671 359 159 2042
Fragments arranged by size:
2155 2042 671 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2160 2200 2260 2374 3053 3450 4127
Size: 516 38 40 60 114 679 397 677
Cuts at: 4127 5275 5520 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 679 677 516 397 245 114
60 40 38
NarI GG'CG_CC
Cuts at: 2166 2166
Size: 6034
NcoI C'CATG_G
Cuts at: 513 1065 2064 2745 513
Size: 552 999 681 3802
Fragments arranged by size:
3802 999 681 552
NdeI CA'TA_TG
Cuts at: 387 3100 3322 387
Size: 2713 222 3099
Fragments arranged by size:
3099 2713 222
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6034
NotI GC'GGCC_GC
Cuts at: 3481 3481
Size: 6034
NspI r_CATG'y
Cuts at: 1132 1229 2690 3392 4937 1132
Size: 97 1461 702 1545 2229
Fragments arranged by size:
2229 1545 1461 702 97
PasI CC'CwG_GG
Cuts at: 1348 2907 1348
Size: 1559 4475
PciI A'CATG_T
Cuts at: 4933 4933
Size: 6034
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2746 1108
Size: 1638 4396
PmeI GTTT'AAAC
Cuts at: 3338 3338
Size: 6034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 5616 5648 4103
Size: 32 1481 32 4489
Fragments arranged by size:
4489 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2222 2222
Size: 6034
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6034
PsiI TTA'TAA
Cuts at: 3604 3604
Size: 6034
PspXI vC'TCGA_Gb
Cuts at: 2994 2994
Size: 6034
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2495 2527 2495
Size: 32 6002
PstI C_TGCA'G
Cuts at: 830 2568 3386 830
Size: 1738 818 3478
Fragments arranged by size:
3478 1738 818
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6034
PvuII CAG'CTG
Cuts at: 2374 2374
Size: 6034
SacII CC_GC'GG
Cuts at: 2161 2161
Size: 6034
SalI G'TCGA_C
Cuts at: 1936 3376 1936
Size: 1440 4594
SanDI GG'GwC_CC
Cuts at: 2222 2222
Size: 6034
SapI GCTCTTCn'nnn_
Cuts at: 2628 2628
Size: 6034
SbfI CC_TGCA'GG
Cuts at: 3386 3386
Size: 6034
ScaI AGT'ACT
Cuts at: 4282 5994 4282
Size: 1712 4322
SfcI C'TryA_G
Cuts at: 826 1045 2564 3382 4517 5198 5389 826
Size: 219 1519 818 1135 681 191 1471
Fragments arranged by size:
1519 1471 1135 818 681 219 191
SfoI GGC'GCC
Cuts at: 2167 2167
Size: 6034
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6034
SmaI CCC'GGG
Cuts at: 1872 2138 2171 3361 1872
Size: 266 33 1190 4545
Fragments arranged by size:
4545 1190 266 33
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2994 4138 5039 5301 820
Size: 197 926 1051 1144 901 262 1553
Fragments arranged by size:
1553 1144 1051 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3825 493
Size: 3332 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6034
SphI G_CATG'C
Cuts at: 1132 3392 1132
Size: 2260 3774
SrfI GCCC'GGGC
Cuts at: 2171 2171
Size: 6034
SspI AAT'ATT
Cuts at: 5 52 3191 3958 5
Size: 47 3139 767 2081
Fragments arranged by size:
3139 2081 767 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6034
StyI C'CwwG_G
Cuts at: 513 1065 2064 2625 2745 3473 513
Size: 552 999 561 120 728 3074
Fragments arranged by size:
3074 999 728 561 552 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2275 3333 4061 4078 4231 4416 4861
Size: 1255 695 1058 728 17 153 185 445
Cuts at: 4861 325
Size: 1498
Fragments arranged by size:
1498 1255 1058 728 695 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4280 5992 96
Size: 274 80 33 51 1443 2303 1712 138
Fragments arranged by size:
2303 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2876 5994 6026 2844
Size: 32 3118 32 2852
Fragments arranged by size:
3118 2852 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2104 1316
Size: 788 5246
XbaI T'CTAG_A
Cuts at: 2809 3370 2809
Size: 561 5473
XhoI C'TCGA_G
Cuts at: 1943 2994 1943
Size: 1051 4983
XmaI C'CCGG_G
Cuts at: 1870 2136 2169 3359 1870
Size: 266 33 1190 4545
Fragments arranged by size:
4545 1190 266 33
XmnI GAAnn'nnTTC
Cuts at: 4163 4163
Size: 6034
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3842 276
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI RsrII SacI SexAI SfiI SwaI
XcmI