Restriction map for
pFN21AB6641
(Circular) (Six-base) MAPSORT of: pFN21AB6641.seq Check: 7684 from: 1 to: 5959
pFN21AB6641 5959 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2788 2788
Size: 5959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2928 3302 1937
Size: 991 374 4594
Fragments arranged by size:
4594 991 374
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 2166 4086 4459 1563
Size: 603 1920 373 3063
Fragments arranged by size:
3063 1920 603 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2484 4022 5406 5674 1632
Size: 852 1538 1384 268 1917
Fragments arranged by size:
1917 1538 1384 852 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 2687 1166
Size: 1521 4438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2705 1184
Size: 1521 4438
AlwNI CAG_nnn'CTG
Cuts at: 2833 2845 3702 5274 2833
Size: 12 857 1572 3518
Fragments arranged by size:
3518 1572 857 12
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2911 3254 3268 3494 3646 5755 1493
Size: 1418 343 14 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 343 226 152 14
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2800 3280 4735 618
Size: 325 217 627 1013 480 1455 1842
Fragments arranged by size:
1842 1455 1013 627 480 325 217
BanII G_rGCy'C
Cuts at: 1391 2184 3278 1391
Size: 793 1094 4072
Fragments arranged by size:
4072 1094 793
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2093 3851 5061 2093
Size: 1758 1210 2991
Fragments arranged by size:
2991 1758 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BfrBI ATG'CAT
Cuts at: 2818 2818
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 2126 1318
Size: 808 5151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2911 3259 3690
Size: 416 53 83 399 24 1494 348 431
Cuts at: 3690 4648 442
Size: 958 1753
Fragments arranged by size:
1753 1494 958 431 416 399 348 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2944 3177 4619 1239
Size: 1705 233 1442 2579
Fragments arranged by size:
2579 1705 1442 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2467 4084 4949 5247 2467
Size: 1617 865 298 3179
Fragments arranged by size:
3179 1617 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2934 2964 3004 3034 1178
Size: 30 165 30 1531 30 40 30 4103
Fragments arranged by size:
4103 1531 165 40 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5959
BseYI C'CCAG_C
Cuts at: 5162 5658 5162
Size: 496 5463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2011 2513 2711 1163
Size: 573 275 502 198 4411
Fragments arranged by size:
4411 573 502 275 198
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2184 4020 4105 5176 2184
Size: 1836 85 1071 2967
Fragments arranged by size:
2967 1836 1071 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 3278 4020 4105 5176 1391
Size: 399 394 1094 742 85 1071 2174
Fragments arranged by size:
2174 1094 1071 742 399 394 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2788 3314 844
Size: 1944 526 3489
Fragments arranged by size:
3489 1944 526
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2299 3185 4454 4628 65
Size: 1183 1051 886 1269 174 1396
Fragments arranged by size:
1396 1269 1183 1051 886 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 3061 3289 3656 4041 4058 5510 5596 5608
Size: 1089 228 367 385 17 1452 86 12
Cuts at: 5608 5954 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1089 385 367 346 228 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2247 2696 513
Size: 552 1182 449 3776
Fragments arranged by size:
3776 1182 552 449
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3406 4295 5924
Size: 54 1425 50 275 18 1576 889 1629
Cuts at: 5924 8
Size: 43
Fragments arranged by size:
1629 1576 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2194 3889 1353
Size: 841 1695 3423
Fragments arranged by size:
3423 1695 841
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2182 2182
Size: 5959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2484 2944 3177 4022 4619 5406 5674
Size: 393 852 460 233 845 597 787 268
Cuts at: 5674 1239
Size: 1524
Fragments arranged by size:
1524 852 845 787 597 460 393 268
233
EcoO109I rG'GnC_Cy
Cuts at: 2451 2852 2451
Size: 401 5558
EcoRI G'AATT_C
Cuts at: 3254 3268 3646 3254
Size: 14 378 5567
Fragments arranged by size:
5567 378 14
EcoRV GAT'ATC
Cuts at: 2875 2875
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373
Size: 32 135 32 3028 32 42 32 2626
Fragments arranged by size:
3028 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2441 3303 3549 669
Size: 988 281 503 862 246 3079
Fragments arranged by size:
3079 988 862 503 281 246
HindIII A'AGCT_T
Cuts at: 748 2310 748
Size: 1562 4397
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2535 2687 3917 4276 4435 518
Size: 648 1369 152 1230 359 159 2042
Fragments arranged by size:
2042 1369 1230 648 359 159 152
MspA1I CmG'CkG
Cuts at: 1606 2394 3375 4052 5200 5445 1606
Size: 788 981 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 788 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NsiI A_TGCA'T
Cuts at: 2820 2820
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 2781 2818 3317 4862 1132
Size: 97 1552 37 499 1545 2229
Fragments arranged by size:
2229 1552 1545 499 97 37
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2808 2808
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2451 2852 2451
Size: 401 5558
PshAI GACnn'nnGTC
Cuts at: 1613 2222 1613
Size: 609 5350
PsiI TTA'TAA
Cuts at: 3168 3529 3168
Size: 361 5598
PstI C_TGCA'G
Cuts at: 830 3311 830
Size: 2481 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2394 2394
Size: 5959
SacI G_AGCT'C
Cuts at: 2184 2184
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 2151 4207 5919 2151
Size: 2056 1712 2191
Fragments arranged by size:
2191 2056 1712
SfcI C'TryA_G
Cuts at: 826 1045 3307 4442 5123 5314 826
Size: 219 2262 1135 681 191 1471
Fragments arranged by size:
2262 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2446 4063 4964 5226 820
Size: 197 926 503 1617 901 262 1553
Fragments arranged by size:
1617 1553 926 901 503 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 2359 3883 5
Size: 47 2307 1524 2081
Fragments arranged by size:
2307 2081 1524 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 3203 3398 513
Size: 552 2138 195 3074
Fragments arranged by size:
3074 2138 552 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3003 3986 4003 4156 4341 4786 325
Size: 1255 1423 983 17 153 185 445 1498
Fragments arranged by size:
1498 1423 1255 983 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2149 4205 5917
Size: 274 80 33 51 1443 172 2056 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
2056 1712 1443 274 172 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2934 2966 2987 3019 5919 5951 2934
Size: 32 21 32 2900 32 2942
Fragments arranged by size:
2942 2900 32 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2115 2254 3201 2011
Size: 104 139 947 4769
Fragments arranged by size:
4769 947 139 104
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI