Restriction map for
pFN21AB6640
(Circular) (Six-base) MAPSORT of: pFN21AB6640.seq Check: 9671 from: 1 to: 6031
pFN21AB6640 6031 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 278
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3374 1937
Size: 1437 4594
Acc65I G'GTAC_C
Cuts at: 3352 3352
Size: 6031
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2451 2583 4094 5478 5746 1632
Size: 819 132 1511 1384 268 1917
Fragments arranged by size:
1917 1511 1384 819 268 132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 2287 4930 5907 1203
Size: 1084 2643 977 1327
Fragments arranged by size:
2643 1327 1084 977
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6031
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2145 1184
Size: 961 5070
AlwNI CAG_nnn'CTG
Cuts at: 3774 5346 3774
Size: 1572 4459
ApaLI G'TGCA_C
Cuts at: 4088 5244 4088
Size: 1156 4875
ApoI r'AATT_y
Cuts at: 1493 2715 2724 2904 3169 3340 3566 3718 5827
Size: 1222 9 180 265 171 226 152 2109
Cuts at: 5827 1493
Size: 1697
Fragments arranged by size:
2109 1697 1222 265 226 180 171 152
9
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 3356 1870
Size: 73 1413 4545
Fragments arranged by size:
4545 1413 73
BamHI G'GATC_C
Cuts at: 3361 3728 3361
Size: 367 5664
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2858 3352 4807 618
Size: 325 217 627 1071 494 1455 1842
Fragments arranged by size:
1842 1455 1071 627 494 325 217
BanII G_rGCy'C
Cuts at: 1391 2317 3350 1391
Size: 926 1033 4072
Fragments arranged by size:
4072 1033 926
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2439 2520 928
Size: 971 540 81 4439
Fragments arranged by size:
4439 971 540 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4222 4256 1903
Size: 34 2285 34 3678
Fragments arranged by size:
3678 2285 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3923 5133 3923
Size: 1210 4821
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4642 136
Size: 107 122 71 631 605 2970 1525
Fragments arranged by size:
2970 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6026 6026
Size: 6031
BlpI GC'TnA_GC
Cuts at: 2316 3448 2316
Size: 1132 4899
Bme1580I G_kGCm'C
Cuts at: 1790 3280 4092 5248 1790
Size: 1490 812 1156 2573
Fragments arranged by size:
2573 1490 1156 812
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6031
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2051 3762 4720
Size: 416 53 83 399 24 634 1711 958
Cuts at: 4720 442
Size: 1753
Fragments arranged by size:
1753 1711 958 634 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2552 1056
Size: 1496 4535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4691 1239
Size: 3452 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4156 5021 5319 4156
Size: 865 298 4868
Fragments arranged by size:
4868 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2039 4694 882
Size: 617 540 2655 2219
Fragments arranged by size:
2655 2219 617 540
BsaAI yAC'GTr
Cuts at: 493 1204 3275 3822 493
Size: 711 2071 547 2702
Fragments arranged by size:
2702 2071 711 547
BsaBI GATnn'nnATC
Cuts at: 6025 6025
Size: 6031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3838 4220 4855 275
Size: 53 83 186 936 2305 382 635 1451
Fragments arranged by size:
2305 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4464 4862 5136 5283 5694 4464
Size: 398 274 147 411 4801
Fragments arranged by size:
4801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5806
Fragments arranged by size:
5806 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3312 1186
Size: 2126 3905
BseYI C'CCAG_C
Cuts at: 5234 5730 5234
Size: 496 5535
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5458
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3481 4242 5270 1540
Size: 275 186 1480 761 1028 2301
Fragments arranged by size:
2301 1480 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4092 4177 5248 4092
Size: 85 1071 4875
Fragments arranged by size:
4875 1071 85
BsmI GAATG_Cn'
Cuts at: 2693 3542 3635 2693
Size: 849 93 5089
Fragments arranged by size:
5089 849 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2317 3280 3350 4092 4177 5248 1391
Size: 399 527 963 70 742 85 1071 2174
Fragments arranged by size:
2174 1071 963 742 527 399 85 70
BspEI T'CCGG_A
Cuts at: 5694 5694
Size: 6031
BspHI T'CATG_A
Cuts at: 3920 5650 3920
Size: 1730 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3092 3386 844
Size: 2248 294 3489
Fragments arranged by size:
3489 2248 294
BsrBI CCG'CTC
Cuts at: 1394 1823 3918 1394
Size: 429 2095 3507
Fragments arranged by size:
3507 2095 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4526 4700 65
Size: 1183 3278 174 1396
Fragments arranged by size:
3278 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4675 4862 1700
Size: 110 2865 187 2869
Fragments arranged by size:
2869 2865 187 110
BsrGI T'GTAC_A
Cuts at: 96 3019 96
Size: 2923 3108
BssHII G'CGCG_C
Cuts at: 1925 2026 1925
Size: 101 5930
BssSI C'ACGA_G
Cuts at: 4091 5103 4091
Size: 1012 5019
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2270 3160 794
Size: 1476 890 3665
Fragments arranged by size:
3665 1476 890
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstYI r'GATC_y
Cuts at: 1972 3361 3728 4113 4130 5582 5668 5680 6026
Size: 1389 367 385 17 1452 86 12 346
Cuts at: 6026 1972
Size: 1977
Fragments arranged by size:
1977 1452 1389 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2016 513
Size: 552 951 4528
Fragments arranged by size:
4528 951 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5292
Fragments arranged by size:
5292 683 56
BtsI GCAGTG_nn'
Cuts at: 2529 2571 3547 4341 4361 2529
Size: 42 976 794 20 4199
Fragments arranged by size:
4199 976 794 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 3335 3335
Size: 6031
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5038 809
Size: 507 3722 1802
Fragments arranged by size:
3722 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2275 3478 4367
Size: 54 1425 50 275 18 445 1203 889
Cuts at: 4367 5996 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1203 889 445 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3478 1537
Size: 275 1666 4090
Fragments arranged by size:
4090 1666 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2599 2808 3961 1353
Size: 1246 209 1153 3423
Fragments arranged by size:
3423 1246 1153 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4618 4992 5138 387
Size: 1064 138 3029 374 146 1280
Fragments arranged by size:
3029 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 2583 4094 4691 5478 5746 1239
Size: 393 819 132 1511 597 787 268 1524
Fragments arranged by size:
1524 1511 819 787 597 393 268 132
EcoNI CCTnn'n_nnAGG
Cuts at: 2870 2870
Size: 6031
EcoO109I rG'GnC_Cy
Cuts at: 3259 3259
Size: 6031
EcoRI G'AATT_C
Cuts at: 2904 3340 3718 2904
Size: 436 378 5217
Fragments arranged by size:
5217 436 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 6031
FspI TGC'GCA
Cuts at: 4537 4537
Size: 6031
HaeII r_GCGC'y
Cuts at: 1678 5178 1678
Size: 3500 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2510 2542 4672 4704 4746
Size: 32 135 32 938 32 2130 32 42
Cuts at: 4746 4778 1373
Size: 32 2626
Fragments arranged by size:
2626 2130 938 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3241 3375 3621 669
Size: 988 281 1303 134 246 3079
Fragments arranged by size:
3079 1303 988 281 246 134
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6031
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KpnI G_GTAC'C
Cuts at: 3356 3356
Size: 6031
MfeI C'AATT_G
Cuts at: 2462 3195 3630 2462
Size: 733 435 4863
Fragments arranged by size:
4863 733 435
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5145 5329 1305
Size: 3840 184 2007
Fragments arranged by size:
3840 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2277 10
Size: 54 1425 343 445 3764
Fragments arranged by size:
3764 1425 445 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2665 3989 4348 4507 518
Size: 648 1499 1324 359 159 2042
Fragments arranged by size:
2042 1499 1324 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 3447 4124 5272 5517 1606
Size: 412 1429 677 1148 245 2120
Fragments arranged by size:
2120 1429 1148 677 412 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5479
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6031
NheI G'CTAG_C
Cuts at: 1052 2548 1052
Size: 1496 4535
NotI GC'GGCC_GC
Cuts at: 3478 3478
Size: 6031
NspI r_CATG'y
Cuts at: 1132 1229 2291 3389 4934 1132
Size: 97 1062 1098 1545 2229
Fragments arranged by size:
2229 1545 1098 1062 97
PasI CC'CwG_GG
Cuts at: 1348 2708 1348
Size: 1360 4671
PciI A'CATG_T
Cuts at: 2287 4930 2287
Size: 2643 3388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6031
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5613 5645 4100
Size: 32 1481 32 4486
Fragments arranged by size:
4486 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3259 3259
Size: 6031
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6031
PsiI TTA'TAA
Cuts at: 2490 3601 2490
Size: 1111 4920
PstI C_TGCA'G
Cuts at: 830 2266 3383 830
Size: 1436 1117 3478
Fragments arranged by size:
3478 1436 1117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 6031
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SbfI CC_TGCA'GG
Cuts at: 3383 3383
Size: 6031
ScaI AGT'ACT
Cuts at: 4279 5991 4279
Size: 1712 4319
SfcI C'TryA_G
Cuts at: 826 1045 2262 3379 4514 5195 5386 826
Size: 219 1217 1117 1135 681 191 1471
Fragments arranged by size:
1471 1217 1135 1117 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 3358 1872
Size: 1486 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4135 5036 5298 820
Size: 197 926 2192 901 262 1553
Fragments arranged by size:
2192 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 3007 152
Size: 2855 3176
SphI G_CATG'C
Cuts at: 1132 3389 1132
Size: 2257 3774
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6031
StyI C'CwwG_G
Cuts at: 513 1065 3470 513
Size: 552 2405 3074
Fragments arranged by size:
3074 2405 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2053 4058 4075 4228 4413 4858 325
Size: 1255 473 2005 17 153 185 445 1498
Fragments arranged by size:
2005 1498 1255 473 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2390 2817 3019
Size: 274 80 33 51 1443 413 427 202
Cuts at: 3019 4277 5989 96
Size: 1258 1712 138
Fragments arranged by size:
1712 1443 1258 427 413 274 202 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5991 6023 5991
Size: 32 5999
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3292 3367 3292
Size: 75 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 3356 1870
Size: 1486 4545
XmnI GAAnn'nnTTC
Cuts at: 2789 3063 4160 2789
Size: 274 1097 4660
Fragments arranged by size:
4660 1097 274
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 276
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI