Restriction map for
pFN21AB6637
(Circular) (Six-base) MAPSORT of: pFN21AB6637.seq Check: 693 from: 1 to: 5959
pFN21AB6637 5959 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2164 2468 2993 2995 3077 3132 4022 5406
Size: 532 304 525 2 82 55 890 1384
Cuts at: 5406 5674 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 890 532 525 304 268 82
55 2
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 2920 4688 2920
Size: 1768 4191
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2051 2065 1184
Size: 867 14 5078
Fragments arranged by size:
5078 867 14
AlwNI CAG_nnn'CTG
Cuts at: 2863 3702 5274 2863
Size: 839 1572 3548
Fragments arranged by size:
3548 1572 839
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2317 3268 3494 3646 5755 1493
Size: 824 951 226 152 2109 1697
Fragments arranged by size:
2109 1697 951 824 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3161 3284 1870
Size: 73 1218 123 4545
Fragments arranged by size:
4545 1218 123 73
BamHI G'GATC_C
Cuts at: 2080 3289 3656 2080
Size: 1209 367 4383
Fragments arranged by size:
4383 1209 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2728 3049 3280 4735 618
Size: 325 217 627 941 321 231 1455 1842
Fragments arranged by size:
1842 1455 941 627 325 321 231 217
BanII G_rGCy'C
Cuts at: 1391 3278 1391
Size: 1887 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2004 2111 2919 3001 3181 928
Size: 971 105 107 808 82 180 3706
Fragments arranged by size:
3706 971 808 180 107 105 82
BbvCI CC'TCA_GC
Cuts at: 2076 2435 2076
Size: 359 5600
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4150 4184 1903
Size: 34 50 34 2129 34 3678
Fragments arranged by size:
3678 2129 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2125 1548
Size: 577 5382
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2231 3002 3690
Size: 416 53 83 399 24 814 771 688
Cuts at: 3690 4648 442
Size: 958 1753
Fragments arranged by size:
1753 958 814 771 688 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2390 4619 1239
Size: 1088 63 2229 2579
Fragments arranged by size:
2579 2229 1088 63
Bpu10I CC'TnA_GC
Cuts at: 2076 2435 2076
Size: 359 5600
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2556 4084 4949 5247 2556
Size: 1528 865 298 3268
Fragments arranged by size:
3268 1528 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2656 4622 882
Size: 617 1157 1966 2219
Fragments arranged by size:
2219 1966 1157 617
BsaAI yAC'GTr
Cuts at: 493 1204 2557 2704 3750 493
Size: 711 1353 147 1046 2702
Fragments arranged by size:
2702 1353 1046 711 147
BsaBI GATnn'nnATC
Cuts at: 2130 5953 2130
Size: 3823 2136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2338 4392 4790 5064 5211 5622 2338
Size: 2054 398 274 147 411 2675
Fragments arranged by size:
2675 2054 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3202 3232 1178
Size: 30 165 30 1799 30 3905
Fragments arranged by size:
3905 1799 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 3059 1186
Size: 1177 696 4086
Fragments arranged by size:
4086 1177 696
BseYI C'CCAG_C
Cuts at: 2787 3119 5162 5658 2787
Size: 332 2043 496 3088
Fragments arranged by size:
3088 2043 496 332
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4020 4105 5176 4020
Size: 85 1071 4803
Fragments arranged by size:
4803 1071 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3278 4020 4105 5176 1391
Size: 399 1488 742 85 1071 2174
Fragments arranged by size:
2174 1488 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2338 5622 2338
Size: 3284 2675
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3314 844
Size: 2470 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2268 2428 2821 3022 4454 4628 65
Size: 1183 1020 160 393 201 1432 174 1396
Fragments arranged by size:
1432 1396 1183 1020 393 201 174 160
BsrFI r'CCGG_y
Cuts at: 1700 1810 2417 4603 4790 1700
Size: 110 607 2186 187 2869
Fragments arranged by size:
2869 2186 607 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 2020 4019 5031 2020
Size: 1999 1012 2948
Fragments arranged by size:
2948 1999 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 2713 1184
Size: 1529 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 2902 2902
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2080 2349 3289 3656 4041 4058 5510 5596
Size: 108 269 940 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 940 385 367 346 269 108
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5407
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2230 487
Size: 683 56 1004 4216
Fragments arranged by size:
4216 1004 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 2647 3263 2647
Size: 616 5343
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3406 4295 5924
Size: 54 1425 50 275 18 1576 889 1629
Cuts at: 5924 8
Size: 43
Fragments arranged by size:
1629 1576 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2523 2685 2969 3230 3889 1353
Size: 1170 162 284 261 659 3423
Fragments arranged by size:
3423 1170 659 284 261 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2164 2327 2390 2468 2993 2995 3077
Size: 393 532 163 63 78 525 2 82
Cuts at: 3077 3132 4022 4619 5406 5674 1239
Size: 55 890 597 787 268 1524
Fragments arranged by size:
1524 890 787 597 532 525 393 268
163 82 78 63 55 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 2438 2075
Size: 363 5596
EcoO109I rG'GnC_Cy
Cuts at: 2311 2443 2311
Size: 132 5827
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
EcoRV GAT'ATC
Cuts at: 2887 2887
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2906 2938 4600 4632 4674
Size: 32 135 32 1334 32 1662 32 42
Cuts at: 4674 4706 1373
Size: 32 2626
Fragments arranged by size:
2626 1662 1334 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2512 2990 3303 3549 669
Size: 988 281 574 478 313 246 3079
Fragments arranged by size:
3079 988 574 478 313 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2235 5073 5257 1305
Size: 930 2838 184 2007
Fragments arranged by size:
2838 2007 930 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3146 3917 4276 4435 518
Size: 648 1980 771 359 159 2042
Fragments arranged by size:
2042 1980 771 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 2410 2787 3375 4052 5200 5445 1606
Size: 591 213 377 588 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 591 588 377 245 213
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 2755 3317 4862 1132
Size: 97 1526 562 1545 2229
Fragments arranged by size:
2229 1545 1526 562 97
PasI CC'CwG_GG
Cuts at: 1348 2086 2272 1348
Size: 738 186 5035
Fragments arranged by size:
5035 738 186
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2304 2364 2901 2304
Size: 60 537 5362
Fragments arranged by size:
5362 537 60
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PmlI CAC'GTG
Cuts at: 2704 2704
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 4028 4060 5541 5573 3155
Size: 32 841 32 1481 32 3541
Fragments arranged by size:
3541 1481 841 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 3311 830
Size: 2481 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2197 2410 2787 2197
Size: 213 377 5369
Fragments arranged by size:
5369 377 213
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SapI GCTCTTCn'nnn_
Cuts at: 2969 2969
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SexAI A'CCwGG_T
Cuts at: 2709 2709
Size: 5959
SfcI C'TryA_G
Cuts at: 826 1045 2250 2855 3307 4442 5123 5314 826
Size: 219 1205 605 452 1135 681 191 1471
Fragments arranged by size:
1471 1205 1135 681 605 452 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2571 4063 4964 5226 820
Size: 197 926 628 1492 901 262 1553
Fragments arranged by size:
1553 1492 926 901 628 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 2046 1517
Size: 529 5430
StyI C'CwwG_G
Cuts at: 513 1065 2280 3066 3398 513
Size: 552 1215 786 332 3074
Fragments arranged by size:
3074 1215 786 552 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2151 2779 2894 3266 3986 4003 4156
Size: 1255 571 628 115 372 720 17 153
Cuts at: 4156 4341 4786 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 720 628 571 445 372 185
153 115 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4205 5917 96
Size: 274 80 33 51 1443 2228 1712 138
Fragments arranged by size:
2228 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2320 2352 3225 3257 5919 5951 2320
Size: 32 873 32 2662 32 2328
Fragments arranged by size:
2662 2328 873 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI XcmI