Restriction map for
pFN21AB6633
(Circular) (Six-base) MAPSORT of: pFN21AB6633.seq Check: 4312 from: 1 to: 5956
pFN21AB6633 5956 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2152 2152
Size: 5956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 2598 3277 2598
Size: 679 5277
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2973 4019 5403 5671 1632
Size: 1341 1046 1384 268 1917
Fragments arranged by size:
1917 1384 1341 1046 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 2684 1166
Size: 1518 4438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AlwNI CAG_nnn'CTG
Cuts at: 3699 5271 3699
Size: 1572 4384
ApaLI G'TGCA_C
Cuts at: 2060 4013 5169 2060
Size: 1953 1156 2847
Fragments arranged by size:
2847 1953 1156
ApoI r'AATT_y
Cuts at: 1493 3265 3491 3643 5752 1493
Size: 1772 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2218 3281 1870
Size: 73 90 185 1063 4545
Fragments arranged by size:
4545 1063 185 90 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2130 2222 2417 2598
Size: 325 217 627 336 7 92 195 181
Cuts at: 2598 3277 4732 618
Size: 679 1455 1842
Fragments arranged by size:
1842 1455 679 627 336 325 217 195
181 92 7
BanII G_rGCy'C
Cuts at: 1391 2189 2407 3275 1391
Size: 798 218 868 4072
Fragments arranged by size:
4072 868 798 218
BbeI G_GCGC'C
Cuts at: 2127 2134 2226 2421 2127
Size: 7 92 195 5662
Fragments arranged by size:
5662 195 92 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2988 928
Size: 971 1089 3896
Fragments arranged by size:
3896 1089 971
BbvCI CC'TCA_GC
Cuts at: 2202 2202
Size: 5956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4147 4181 1903
Size: 34 50 34 2126 34 3678
Fragments arranged by size:
3678 2126 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2603 3848 5058 2603
Size: 1245 1210 3501
Fragments arranged by size:
3501 1245 1210
BclI T'GATC_A
Cuts at: 1548 2776 1548
Size: 1228 4728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4567 136
Size: 107 122 71 631 605 2895 1525
Fragments arranged by size:
2895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 2177 3373 2177
Size: 1196 4760
Bme1580I G_kGCm'C
Cuts at: 1790 2064 4017 5173 1790
Size: 274 1953 1156 2573
Fragments arranged by size:
2573 1953 1156 274
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3687 4645 442
Size: 416 53 83 399 24 2270 958 1753
Fragments arranged by size:
2270 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2355 4616 1239
Size: 1116 2261 2579
Fragments arranged by size:
2579 2261 1116
Bpu10I CC'TnA_GC
Cuts at: 2202 2202
Size: 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2642 4619 882
Size: 617 1143 1977 2219
Fragments arranged by size:
2219 1977 1143 617
BsaAI yAC'GTr
Cuts at: 493 1204 2682 2686 3747 493
Size: 711 1478 4 1061 2702
Fragments arranged by size:
2702 1478 1061 711 4
BsaBI GATnn'nnATC
Cuts at: 2775 5950 2775
Size: 3175 2781
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 2131 2223 2418
Size: 53 83 186 936 591 7 92 195
Cuts at: 2418 2675 3763 4145 4780 275
Size: 257 1088 382 635 1451
Fragments arranged by size:
1451 1088 936 635 591 382 257 195
186 92 83 53 7
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5731
Fragments arranged by size:
5731 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2956 1186
Size: 1770 4186
BseYI C'CCAG_C
Cuts at: 2256 2483 2840 3149 5159 5655 2256
Size: 227 357 309 2010 496 2557
Fragments arranged by size:
2557 2010 496 357 309 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2259 2708 3012 3093 3223 1163
Size: 573 523 449 304 81 130 3896
Fragments arranged by size:
3896 573 523 449 304 130 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2325 2480 3406 4167 5195 1540
Size: 275 186 324 155 926 761 1028 2301
Fragments arranged by size:
2301 1028 926 761 324 275 186 155
BsiHKAI G_wGCw'C
Cuts at: 2028 2064 4017 4102 5173 2028
Size: 36 1953 85 1071 2811
Fragments arranged by size:
2811 1953 1071 85 36
BsiWI C'GTAC_G
Cuts at: 2682 2682
Size: 5956
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2064 2189 2407 3275 4017 4102
Size: 399 238 36 125 218 868 742 85
Cuts at: 4102 5173 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 868 742 399 238 218 125
85 36
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 2481 2586 2928 3311 844
Size: 1308 329 105 342 383 3489
Fragments arranged by size:
3489 1308 383 342 329 105
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 2110 2324 2601 4600 4787 1700
Size: 110 271 29 214 277 1999 187 2869
Fragments arranged by size:
2869 1999 277 271 214 187 110 29
BsrGI T'GTAC_A
Cuts at: 96 2703 96
Size: 2607 3349
BssHII G'CGCG_C
Cuts at: 1925 2052 2425 1925
Size: 127 373 5456
Fragments arranged by size:
5456 373 127
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3026 794
Size: 2232 3724
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2491 3157 2491
Size: 666 5290
BstYI r'GATC_y
Cuts at: 1972 2139 3286 3653 4038 4055 5507 5593 5605
Size: 167 1147 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1147 385 367 346 167 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3232 3232
Size: 5956
BtgI C'CryG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 4165
Fragments arranged by size:
4165 1239 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 2062 3472 4266 4286 2062
Size: 1410 794 20 3732
Fragments arranged by size:
3732 1410 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DraIII CAC_nnn'GTG
Cuts at: 2785 2785
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2322 2347
Size: 54 1425 50 275 18 253 239 25
Cuts at: 2347 2477 2652 2739 3403 4292 5921 8
Size: 130 175 87 664 889 1629 43
Fragments arranged by size:
1629 1425 889 664 275 253 239 175
130 87 54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2322 2477 3403 1537
Size: 275 510 155 926 4090
Fragments arranged by size:
4090 926 510 275 155
EarI CTCTTCn'nnn_
Cuts at: 1353 3886 1353
Size: 2533 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2242 2738 4543 4917 5063 387
Size: 1064 138 653 496 1805 374 146 1280
Fragments arranged by size:
1805 1280 1064 653 496 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2355 2973 4019 4616 5403 5671 1239
Size: 393 723 618 1046 597 787 268 1524
Fragments arranged by size:
1524 1046 787 723 618 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2215 2215
Size: 5956
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 2127 2134 2209 2226 2421 2447 5103 1678
Size: 449 7 75 17 195 26 2656 2531
Fragments arranged by size:
2656 2531 449 195 75 26 17 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4597 4629 4671 4703 1373
Size: 32 135 32 3025 32 42 32 2626
Fragments arranged by size:
3025 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KasI G'GCGC_C
Cuts at: 2123 2130 2222 2417 2123
Size: 7 92 195 5662
Fragments arranged by size:
5662 195 92 7
KpnI G_GTAC'C
Cuts at: 2602 3281 2602
Size: 679 5277
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2741 10
Size: 54 1425 343 909 3225
Fragments arranged by size:
3225 1425 909 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2684 3225 3914 4273 4432 518
Size: 648 1518 541 689 359 159 2042
Fragments arranged by size:
2042 1518 689 648 541 359 159
MspA1I CmG'CkG
Cuts at: 1606 2306 2345 3372 4049 5197 5442 1606
Size: 700 39 1027 677 1148 245 2120
Fragments arranged by size:
2120 1148 1027 700 677 245 39
NaeI GCC'GGC
Cuts at: 2083 2112 2326 2083
Size: 29 214 5713
Fragments arranged by size:
5713 214 29
NarI GG'CG_CC
Cuts at: 2124 2131 2223 2418 2124
Size: 7 92 195 5662
Fragments arranged by size:
5662 195 92 7
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5404
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2081 2110 2324 2081
Size: 29 214 5713
Fragments arranged by size:
5713 214 29
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 2477 3403 2477
Size: 926 5030
NspI r_CATG'y
Cuts at: 1132 1229 3024 3314 4859 1132
Size: 97 1795 290 1545 2229
Fragments arranged by size:
2229 1795 1545 290 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2896 1108
Size: 1788 4168
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2215 2215
Size: 5956
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PstI C_TGCA'G
Cuts at: 830 3308 830
Size: 2478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
SacII CC_GC'GG
Cuts at: 2307 2307
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SanDI GG'GwC_CC
Cuts at: 2215 2215
Size: 5956
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 2534 4204 5916 2534
Size: 1670 1712 2574
Fragments arranged by size:
2574 1712 1670
SexAI A'CCwGG_T
Cuts at: 2646 3156 2646
Size: 510 5446
SfcI C'TryA_G
Cuts at: 826 1045 3304 4439 5120 5311 826
Size: 219 2259 1135 681 191 1471
Fragments arranged by size:
2259 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2125 2132 2224 2419 2125
Size: 7 92 195 5662
Fragments arranged by size:
5662 195 92 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 2110 1700
Size: 410 5546
SmaI CCC'GGG
Cuts at: 1872 2220 3283 1872
Size: 348 1063 4545
Fragments arranged by size:
4545 1063 348
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4060 4961 5223 820
Size: 197 926 2117 901 262 1553
Fragments arranged by size:
2117 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2818 3395 513
Size: 552 1753 577 3074
Fragments arranged by size:
3074 1753 577 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2951 3983 4000 4153 4338 4783 325
Size: 1255 1371 1032 17 153 185 445 1498
Fragments arranged by size:
1498 1371 1255 1032 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 2703 2744
Size: 274 80 33 51 1443 555 171 41
Cuts at: 2744 3023 4202 5914 96
Size: 279 1179 1712 138
Fragments arranged by size:
1712 1443 1179 555 279 274 171 138
80 51 41 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 2399 3292 2399
Size: 893 5063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2856 2856
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 2218 3281 1870
Size: 348 1063 4545
Fragments arranged by size:
4545 1063 348
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SapI SfiI SrfI SwaI