Restriction map for
pFN21AB6632
(Circular) (Six-base) MAPSORT of: pFN21AB6632.seq Check: 2001 from: 1 to: 6025
pFN21AB6632 6025 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2326 2380 3159 2326
Size: 54 779 5192
Fragments arranged by size:
5192 779 54
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3368 1937
Size: 1431 4594
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2867 4088 5472 5740 1632
Size: 557 678 1221 1384 268 1917
Fragments arranged by size:
1917 1384 1221 678 557 268
AfeI AGC'GCT
Cuts at: 2023 2023
Size: 6025
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 2268 2931 4924 5901 1203
Size: 1065 663 1993 977 1327
Fragments arranged by size:
1993 1327 1065 977 663
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AlwNI CAG_nnn'CTG
Cuts at: 2849 3768 5340 2849
Size: 919 1572 3534
Fragments arranged by size:
3534 1572 919
ApaLI G'TGCA_C
Cuts at: 4082 5238 4082
Size: 1156 4869
ApoI r'AATT_y
Cuts at: 1493 2940 3334 3560 3712 5821 1493
Size: 1447 394 226 152 2109 1697
Fragments arranged by size:
2109 1697 1447 394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2140 2717 3350 1870
Size: 73 197 577 633 4545
Fragments arranged by size:
4545 633 577 197 73
BamHI G'GATC_C
Cuts at: 2607 2803 3355 3722 2607
Size: 196 552 367 4910
Fragments arranged by size:
4910 552 367 196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2257 2294 2648 2678
Size: 325 217 627 255 215 37 354 30
Cuts at: 2678 2782 3128 3346 4801 618
Size: 104 346 218 1455 1842
Fragments arranged by size:
1842 1455 627 354 346 325 255 218
217 215 104 37 30
BanII G_rGCy'C
Cuts at: 1391 2109 3344 1391
Size: 718 1235 4072
Fragments arranged by size:
4072 1235 718
BbeI G_GCGC'C
Cuts at: 2046 2046
Size: 6025
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2234 928
Size: 971 335 4719
Fragments arranged by size:
4719 971 335
BbvCI CC'TCA_GC
Cuts at: 3156 3237 3285 3156
Size: 81 48 5896
Fragments arranged by size:
5896 81 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4216 4250 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 4636 136
Size: 107 122 71 631 605 421 2543 1525
Fragments arranged by size:
2543 1525 631 605 421 122 107 71
BglII A'GATC_T
Cuts at: 3309 6020 3309
Size: 2711 3314
BlpI GC'TnA_GC
Cuts at: 2025 3442 2025
Size: 1417 4608
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2262 2683 3133 4086 5242 1790
Size: 349 123 421 450 953 1156 2573
Fragments arranged by size:
2573 1156 953 450 421 349 123
BmgBI CAC'GTC
Cuts at: 1318 2932 1318
Size: 1614 4411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2300 3282 3756
Size: 416 53 83 399 24 883 982 474
Cuts at: 3756 4714 442
Size: 958 1753
Fragments arranged by size:
1753 982 958 883 474 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2231 2417 2756 2826 2880 3031 4685
Size: 884 108 186 339 70 54 151 1654
Cuts at: 4685 1239
Size: 2579
Fragments arranged by size:
2579 1654 884 339 186 151 108 70
54
Bpu10I CC'TnA_GC
Cuts at: 3156 3231 3237 3285 3156
Size: 75 6 48 5896
Fragments arranged by size:
5896 75 48 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 5015 5313 4150
Size: 865 298 4862
Fragments arranged by size:
4862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3112 4688 882
Size: 617 1613 1576 2219
Fragments arranged by size:
2219 1613 1576 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 2043 3832 4214
Size: 53 83 186 936 483 27 1789 382
Cuts at: 4214 4849 275
Size: 635 1451
Fragments arranged by size:
1789 1451 936 635 483 382 186 83
53 27
BsaWI w'CCGG_w
Cuts at: 2604 4458 4856 5130 5277 5688 2604
Size: 1854 398 274 147 411 2941
Fragments arranged by size:
2941 1854 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2524 2554 3029 3059 1178
Size: 30 165 30 1121 30 475 30 4144
Fragments arranged by size:
4144 1121 475 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2782 2868 3254 1186
Size: 1596 86 386 3957
Fragments arranged by size:
3957 1596 386 86
BseYI C'CCAG_C
Cuts at: 2083 2088 2261 2624 2828 2978 5228 5724 2083
Size: 5 173 363 204 150 2250 496 2384
Fragments arranged by size:
2384 2250 496 363 204 173 150 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2090 2921 1163
Size: 573 354 831 4267
Fragments arranged by size:
4267 831 573 354
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2842 4086 4171 5242 2842
Size: 1244 85 1071 3625
Fragments arranged by size:
3625 1244 1071 85
BsmI GAATG_Cn'
Cuts at: 3536 3629 3536
Size: 93 5932
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2139 2262 2683 2842 3133 3344
Size: 399 319 30 123 421 159 291 211
Cuts at: 3344 4086 4171 5242 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 421 399 319 291 211
159 123 85 30
BspEI T'CCGG_A
Cuts at: 5688 5688
Size: 6025
BspHI T'CATG_A
Cuts at: 2470 3061 3914 5644 2470
Size: 591 853 1730 2851
Fragments arranged by size:
2851 1730 853 591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2326 2380 3159 3380 844
Size: 1482 54 779 221 3489
Fragments arranged by size:
3489 1482 779 221 54
BsrBI CCG'CTC
Cuts at: 1394 1823 3067 3912 1394
Size: 429 1244 845 3507
Fragments arranged by size:
3507 1244 845 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2968 4520 4694 65
Size: 1183 1720 1552 174 1396
Fragments arranged by size:
1720 1552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2384 4669 4856 1700
Size: 110 574 2285 187 2869
Fragments arranged by size:
2869 2285 574 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6025
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 4085 5097 4085
Size: 1012 5013
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6025
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 2607 2803 3309 3355 3722 4107 4124 5576
Size: 635 196 506 46 367 385 17 1452
Cuts at: 5576 5662 5674 6020 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 635 506 385 367 346 196
86 46 17 12
Bsu36I CC'TnA_GG
Cuts at: 3025 3025
Size: 6025
BtgI C'CryG_G
Cuts at: 513 1065 2004 2062 2796 513
Size: 552 939 58 734 3742
Fragments arranged by size:
3742 939 734 552 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 3541 4335 4355 3541
Size: 794 20 5211
Fragments arranged by size:
5211 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DraIII CAC_nnn'GTG
Cuts at: 2068 2678 2068
Size: 610 5415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2278 3472 4361
Size: 54 1425 50 275 18 448 1194 889
Cuts at: 4361 5990 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1194 889 448 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2886 2995 3171 3955 1353
Size: 1533 109 176 784 3423
Fragments arranged by size:
3423 1533 784 176 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3158 3202 4612 4986 5132 387
Size: 1064 138 1569 44 1410 374 146 1280
Fragments arranged by size:
1569 1410 1280 1064 374 146 138 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2189 2231 2417 2756 2826 2867
Size: 393 491 66 42 186 339 70 41
Cuts at: 2867 2880 3031 4088 4685 5472 5740 1239
Size: 13 151 1057 597 787 268 1524
Fragments arranged by size:
1524 1057 787 597 491 393 339 268
186 151 70 66 42 41 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2777 2777
Size: 6025
EcoO109I rG'GnC_Cy
Cuts at: 2284 2770 3262 2284
Size: 486 492 5047
Fragments arranged by size:
5047 492 486
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 2025 2046 5172 1678
Size: 347 21 3126 2531
Fragments arranged by size:
3126 2531 347 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2524 2556 2599 2631 3029
Size: 32 135 32 952 32 43 32 398
Cuts at: 3029 3061 3194 3226 4666 4698 4740 4772 1373
Size: 32 133 32 1440 32 42 32 2626
Fragments arranged by size:
2626 1440 952 398 135 133 43 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3615 669
Size: 988 281 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6025
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KasI G'GCGC_C
Cuts at: 2042 2042
Size: 6025
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5139 5323 1305
Size: 3834 184 2007
Fragments arranged by size:
3834 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4203
Fragments arranged by size:
4203 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 3983 4342 4501 518
Size: 648 1071 1746 359 159 2042
Fragments arranged by size:
2042 1746 1071 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3014 3160 3441 4118 5266 5511 1606
Size: 1408 146 281 677 1148 245 2120
Fragments arranged by size:
2120 1408 1148 677 281 245 146
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 6025
NarI GG'CG_CC
Cuts at: 2043 2043
Size: 6025
NcoI C'CATG_G
Cuts at: 513 1065 2004 2796 513
Size: 552 939 792 3742
Fragments arranged by size:
3742 939 792 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 2272 2348 3383 4928 1132
Size: 97 1043 76 1035 1545 2229
Fragments arranged by size:
2229 1545 1043 1035 97 76
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6025
PciI A'CATG_T
Cuts at: 2268 4924 2268
Size: 2656 3369
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2869 2973 3203 1108
Size: 1761 104 230 3930
Fragments arranged by size:
3930 1761 230 104
PfoI T'CCnGG_A
Cuts at: 2433 2925 3114 2433
Size: 492 189 5344
Fragments arranged by size:
5344 492 189
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PspXI vC'TCGA_Gb
Cuts at: 2140 2140
Size: 6025
PstI C_TGCA'G
Cuts at: 830 2315 2781 3190 3377 830
Size: 1485 466 409 187 3478
Fragments arranged by size:
3478 1485 466 409 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 3014 3160 3014
Size: 146 5879
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SbfI CC_TGCA'GG
Cuts at: 3377 3377
Size: 6025
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SfcI C'TryA_G
Cuts at: 826 1045 2311 2777 3186 3373 4508 5189 5380
Size: 219 1266 466 409 187 1135 681 191
Cuts at: 5380 826
Size: 1471
Fragments arranged by size:
1471 1266 1135 681 466 409 219 191
187
SfoI GGC'GCC
Cuts at: 2044 2044
Size: 6025
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 3352 1872
Size: 1480 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2140 4129 5030 5292 820
Size: 197 926 197 1989 901 262 1553
Fragments arranged by size:
1989 1553 926 901 262 197 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 3383 1132
Size: 2251 3774
SspI AAT'ATT
Cuts at: 5 52 3949 5
Size: 47 3897 2081
Fragments arranged by size:
3897 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6025
StyI C'CwwG_G
Cuts at: 513 1065 2004 2796 3464 513
Size: 552 939 792 668 3074
Fragments arranged by size:
3074 939 792 668 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 4052 4069 4222 4407 4852 325
Size: 1255 702 1770 17 153 185 445 1498
Fragments arranged by size:
1770 1498 1255 702 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4271 5983 96
Size: 274 80 33 51 1443 2294 1712 138
Fragments arranged by size:
2294 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5985 6017 5985
Size: 32 5993
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6025
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XhoI C'TCGA_G
Cuts at: 1943 2140 1943
Size: 197 5828
XmaI C'CCGG_G
Cuts at: 1870 3350 1870
Size: 1480 4545
XmnI GAAnn'nnTTC
Cuts at: 4154 4154
Size: 6025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI