Restriction map for
pFN21AB6631
(Circular) (Six-base) MAPSORT of: pFN21AB6631.seq Check: 8274 from: 1 to: 5917
pFN21AB6631 5917 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3260 1937
Size: 1323 4594
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 3054 4044 4417 1563
Size: 1491 990 373 3063
Fragments arranged by size:
3063 1491 990 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 2414 2483 2552 2621 2690 2759 3980
Size: 713 69 69 69 69 69 69 1221
Cuts at: 3980 5364 5632 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1221 713 268 69 69 69
69 69 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 4816 5793 1203
Size: 3613 977 1327
Fragments arranged by size:
3613 1327 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3169 1184
Size: 1985 3932
AlwNI CAG_nnn'CTG
Cuts at: 2038 2245 2314 2383 2452 2521 2590 2659 2728
Size: 207 69 69 69 69 69 69 69
Cuts at: 2728 3191 3660 5232 2038
Size: 463 469 1572 2723
Fragments arranged by size:
2723 1572 469 463 207 69 69 69
69 69 69 69
ApaI G_GGCC'C
Cuts at: 2872 2872
Size: 5917
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 3077 3122 3226 3452 3604 5713 1493
Size: 1584 45 104 226 152 2109 1697
Fragments arranged by size:
2109 1697 1584 226 152 104 45
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 3242 1870
Size: 73 145 1154 4545
Fragments arranged by size:
4545 1154 145 73
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3238 4693 618
Size: 325 217 627 1451 1455 1842
Fragments arranged by size:
1842 1455 1451 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2172 2277 2872 3048 3236 1391
Size: 781 105 595 176 188 4072
Fragments arranged by size:
4072 781 595 188 176 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2916 3072 928
Size: 971 1017 156 3773
Fragments arranged by size:
3773 1017 971 156
BbvCI CC'TCA_GC
Cuts at: 2895 2895
Size: 5917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2087 3809 5019 2087
Size: 1722 1210 2985
Fragments arranged by size:
2985 1722 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4528 136
Size: 107 122 71 631 605 2856 1525
Fragments arranged by size:
2856 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2301 2370 2439 2508 2577 2646 2715 2962 5912
Size: 69 69 69 69 69 69 247 2950
Cuts at: 5912 2301
Size: 2306
Fragments arranged by size:
2950 2306 247 69 69 69 69 69
69
BlpI GC'TnA_GC
Cuts at: 2104 3334 2104
Size: 1230 4687
Bme1580I G_kGCm'C
Cuts at: 1790 2872 3978 5134 1790
Size: 1082 1106 1156 2573
Fragments arranged by size:
2573 1156 1106 1082
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4606 442
Size: 416 53 83 399 24 2231 958 1753
Fragments arranged by size:
2231 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 3142 3168 4577 1239
Size: 830 1073 26 1409 2579
Fragments arranged by size:
2579 1409 1073 830 26
Bpu10I CC'TnA_GC
Cuts at: 2895 2895
Size: 5917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4042 4907 5205 4042
Size: 865 298 4754
Fragments arranged by size:
4754 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4580 882
Size: 617 3081 2219
Fragments arranged by size:
3081 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2982 3708 493
Size: 711 1778 726 2702
Fragments arranged by size:
2702 1778 726 711
BsaBI GATnn'nnATC
Cuts at: 2967 5911 2967
Size: 2944 2973
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3724 4106 4741 275
Size: 53 83 186 936 2191 382 635 1451
Fragments arranged by size:
2191 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4350 4748 5022 5169 5580 4350
Size: 398 274 147 411 4687
Fragments arranged by size:
4687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5692
Fragments arranged by size:
5692 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2135 2912 3054 1186
Size: 880 69 777 142 4049
Fragments arranged by size:
4049 880 777 142 69
BseYI C'CCAG_C
Cuts at: 2038 2165 2195 2250 2319 2733 2823 2871 3014
Size: 127 30 55 69 414 90 48 143
Cuts at: 3014 5120 5616 2038
Size: 2106 496 2339
Fragments arranged by size:
2339 2106 496 414 143 127 90 69
55 48 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 4128 5156 1540
Size: 275 186 1366 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 3978 4063 5134 2277
Size: 1701 85 1071 3060
Fragments arranged by size:
3060 1701 1071 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2277 2872 3048 3236 3978 4063
Size: 399 382 105 595 176 188 742 85
Cuts at: 4063 5134 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 595 399 382 188 176
105 85
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3272 844
Size: 2428 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2993 3094 4412 4586 65
Size: 1183 1745 101 1318 174 1396
Fragments arranged by size:
1745 1396 1318 1183 174 101
BsrFI r'CCGG_y
Cuts at: 1700 1810 4561 4748 1700
Size: 110 2751 187 2869
Fragments arranged by size:
2869 2751 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 2301 2370 2439 2508 2577 2646 2715 2962
Size: 329 69 69 69 69 69 69 247
Cuts at: 2962 3129 3247 3614 3999 4016 5468 5554 5566
Size: 167 118 367 385 17 1452 86 12
Cuts at: 5566 5912 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 329 247 167
118 86 69 69 69 69 69 69
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2122 513
Size: 552 1057 4308
Fragments arranged by size:
4308 1057 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 2271 2340 2409 2478 2547 2616 2685 2754 3433
Size: 69 69 69 69 69 69 69 679
Cuts at: 3433 4227 4247 2271
Size: 794 20 3941
Fragments arranged by size:
3941 794 679 69 69 69 69 69
69 69 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4924 809
Size: 507 3608 1802
Fragments arranged by size:
3608 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 3364 4253
Size: 54 1425 50 275 18 289 1245 889
Cuts at: 4253 5882 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1245 889 289 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3847 1353
Size: 2494 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 2275
Size: 5917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2345 2414 2483 2552 2621 2690
Size: 393 437 276 69 69 69 69 69
Cuts at: 2690 2759 3142 3168 3980 4577 5364 5632 1239
Size: 69 383 26 812 597 787 268 1524
Fragments arranged by size:
1524 812 787 597 437 393 383 276
268 69 69 69 69 69 69 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2051 3157 2048
Size: 3 1106 4808
Fragments arranged by size:
4808 1106 3
EcoO109I rG'GnC_Cy
Cuts at: 2191 2868 2191
Size: 677 5240
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 5064 1678
Size: 3386 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4558 4590 4632 4664 1373
Size: 32 135 32 2986 32 42 32 2626
Fragments arranged by size:
2986 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 3507 669
Size: 988 281 1323 246 3079
Fragments arranged by size:
3079 1323 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2104 5031 5215 1305
Size: 799 2927 184 2007
Fragments arranged by size:
2927 2007 799 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2121 10
Size: 54 1425 343 289 3806
Fragments arranged by size:
3806 1425 343 289 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2948 3875 4234 4393 518
Size: 648 1782 927 359 159 2042
Fragments arranged by size:
2042 1782 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2062 2323 2737 2827 2917 3333 4010
Size: 432 24 261 414 90 90 416 677
Cuts at: 4010 5158 5403 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 432 416 414 261 245
90 90 24
NcoI C'CATG_G
Cuts at: 513 1065 2122 513
Size: 552 1057 4308
Fragments arranged by size:
4308 1057 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 3275 4820 1132
Size: 97 2046 1545 2229
Fragments arranged by size:
2229 2046 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2775 1348
Size: 1427 4490
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3126 1108
Size: 2018 3899
PfoI T'CCnGG_A
Cuts at: 2046 2046
Size: 5917
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PmlI CAC'GTG
Cuts at: 2982 2982
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2191
Size: 5917
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PspOMI G'GGCC_C
Cuts at: 2868 2868
Size: 5917
PspXI vC'TCGA_Gb
Cuts at: 2088 2088
Size: 5917
PstI C_TGCA'G
Cuts at: 830 2034 2266 2298 2335 2367 2404 2436 2473
Size: 1204 232 32 37 32 37 32 37
Cuts at: 2473 2542 2611 2680 2749 2922 3269 830
Size: 69 69 69 69 173 347 3478
Fragments arranged by size:
3478 1204 347 232 173 69 69 69
69 37 37 37 32 32 32
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
PvuII CAG'CTG
Cuts at: 2038 2062 2323 2737 2917 2038
Size: 24 261 414 180 5038
Fragments arranged by size:
5038 414 261 180 24
SacI G_AGCT'C
Cuts at: 2277 2277
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 4165 5877 4165
Size: 1712 4205
SfcI C'TryA_G
Cuts at: 826 1045 2030 2262 2294 2331 2363 2400 2432
Size: 219 985 232 32 37 32 37 32
Cuts at: 2432 2469 2538 2607 2676 2745 2912 2918 3265
Size: 37 69 69 69 69 167 6 347
Cuts at: 3265 4400 5081 5272 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 985 681 347 232 219 191
167 69 69 69 69 37 37 37
32 32 32 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3244 1872
Size: 1372 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2088 4021 4922 5184 820
Size: 197 926 145 1933 901 262 1553
Fragments arranged by size:
1933 1553 926 901 262 197 145
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 2070 1517
Size: 553 5364
StyI C'CwwG_G
Cuts at: 513 1065 2016 2071 2122 2187 2794 3356 513
Size: 552 951 55 51 65 607 562 3074
Fragments arranged by size:
3074 951 607 562 552 65 55 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 2327 2396 2465 2534 2603 2672
Size: 1255 678 69 69 69 69 69 69
Cuts at: 2672 2980 3944 3961 4114 4299 4744 325
Size: 308 964 17 153 185 445 1498
Fragments arranged by size:
1498 1255 964 678 445 308 185 153
69 69 69 69 69 69 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4163 5875 96
Size: 274 80 33 51 1443 2186 1712 138
Fragments arranged by size:
2186 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2744 2776 2966 2998 5877 5909 2744
Size: 32 190 32 2879 32 2752
Fragments arranged by size:
2879 2752 190 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 2088 1943
Size: 145 5772
XmaI C'CCGG_G
Cuts at: 1870 3242 1870
Size: 1372 4545
XmnI GAAnn'nnTTC
Cuts at: 3174 4046 3174
Size: 872 5045
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI