Restriction map for
pFN21AB6630
(Circular) (Six-base) MAPSORT of: pFN21AB6630.seq Check: 6773 from: 1 to: 6025
pFN21AB6630 6025 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3314 3314
Size: 6025
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3368 1937
Size: 1431 4594
Acc65I G'GTAC_C
Cuts at: 3346 3346
Size: 6025
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 3244 4088 5472 5740 1632
Size: 812 800 844 1384 268 1917
Fragments arranged by size:
1917 1384 844 812 800 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 4924 5901 1203
Size: 3721 977 1327
Fragments arranged by size:
3721 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 4754 4754
Size: 6025
AleI CACnn'nnGTG
Cuts at: 1166 3228 1166
Size: 2062 3963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2378 2410 2378
Size: 32 5993
AlwNI CAG_nnn'CTG
Cuts at: 2140 3148 3768 5340 2140
Size: 1008 620 1572 2825
Fragments arranged by size:
2825 1572 1008 620
ApaLI G'TGCA_C
Cuts at: 4082 5238 4082
Size: 1156 4869
ApoI r'AATT_y
Cuts at: 1493 3334 3560 3712 5821 1493
Size: 1841 226 152 2109 1697
Fragments arranged by size:
2109 1841 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2928 4579 160
Size: 872 1896 1651 1606
Fragments arranged by size:
1896 1651 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2272 3350 1870
Size: 73 329 1078 4545
Fragments arranged by size:
4545 1078 329 73
BamHI G'GATC_C
Cuts at: 3355 3722 3355
Size: 367 5658
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3222 3302 3346 4801 618
Size: 325 217 627 1435 80 44 1455 1842
Fragments arranged by size:
1842 1455 1435 627 325 217 80 44
BanII G_rGCy'C
Cuts at: 1391 2086 3344 1391
Size: 695 1258 4072
Fragments arranged by size:
4072 1258 695
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3067 928
Size: 971 1168 3886
Fragments arranged by size:
3886 1168 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4216 4250 1903
Size: 34 50 34 2195 34 3678
Fragments arranged by size:
3678 2195 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2639 4636 136
Size: 107 122 71 631 605 967 1997 1525
Fragments arranged by size:
1997 1525 967 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 2180 2572 3305 4086 5242 1790
Size: 390 392 733 781 1156 2573
Fragments arranged by size:
2573 1156 781 733 392 390
BmgBI CAC'GTC
Cuts at: 1318 2104 1318
Size: 786 5239
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3756 4714 442
Size: 416 53 83 399 24 2339 958 1753
Fragments arranged by size:
2339 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 2137 2753 2784 3215 4685 1239
Size: 861 37 616 31 431 1470 2579
Fragments arranged by size:
2579 1470 861 616 431 37 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 5015 5313 4150
Size: 865 298 4862
Fragments arranged by size:
4862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2163 4688 882
Size: 617 664 2525 2219
Fragments arranged by size:
2525 2219 664 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 6019 6019
Size: 6025
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2592 3832 4214 4849
Size: 53 83 186 936 1059 1240 382 635
Cuts at: 4849 275
Size: 1451
Fragments arranged by size:
1451 1240 1059 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2283 2505 4458 4856 5130 5277 5688 2283
Size: 222 1953 398 274 147 411 2620
Fragments arranged by size:
2620 1953 411 398 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 1178
Size: 30 165 30 1041 30 4729
Fragments arranged by size:
4729 1041 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2449 2596 2723 1186
Size: 1263 147 127 4488
Fragments arranged by size:
4488 1263 147 127
BseYI C'CCAG_C
Cuts at: 2859 5228 5724 2859
Size: 2369 496 3160
Fragments arranged by size:
3160 2369 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2308 2503 3209 3227 1163
Size: 573 572 195 706 18 3961
Fragments arranged by size:
3961 706 573 572 195 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3475 4236 5264 1540
Size: 275 186 1474 761 1028 2301
Fragments arranged by size:
2301 1474 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 4086 4171 5242 2601
Size: 1485 85 1071 3384
Fragments arranged by size:
3384 1485 1071 85
BsmI GAATG_Cn'
Cuts at: 2273 2783 3536 3629 2273
Size: 510 753 93 4669
Fragments arranged by size:
4669 753 510 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2180 2572 2601 3305 3344 4086
Size: 399 296 94 392 29 704 39 742
Cuts at: 4086 4171 5242 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 704 399 392 296 94
85 39 29
BspEI T'CCGG_A
Cuts at: 2283 2505 5688 2283
Size: 222 3183 2620
Fragments arranged by size:
3183 2620 222
BspHI T'CATG_A
Cuts at: 3914 5644 3914
Size: 1730 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2427 3314 3380 844
Size: 1583 887 66 3489
Fragments arranged by size:
3489 1583 887 66
BsrBI CCG'CTC
Cuts at: 1394 1823 3912 1394
Size: 429 2089 3507
Fragments arranged by size:
3507 2089 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3224 4520 4694 65
Size: 1183 1976 1296 174 1396
Fragments arranged by size:
1976 1396 1296 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2047 4669 4856 1700
Size: 110 237 2622 187 2869
Fragments arranged by size:
2869 2622 237 187 110
BsrGI T'GTAC_A
Cuts at: 96 2439 96
Size: 2343 3682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 4085 5097 4085
Size: 1012 5013
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2191 794
Size: 1397 4628
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstXI CCAn_nnnn'nTGG
Cuts at: 3286 3286
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 3105 3355 3722 4107 4124 5576 5662 5674
Size: 1133 250 367 385 17 1452 86 12
Cuts at: 5674 6020 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1133 385 367 346 250 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2445 3249 513
Size: 552 1380 804 3289
Fragments arranged by size:
3289 1380 804 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2643 487
Size: 683 56 1417 3869
Fragments arranged by size:
3869 1417 683 56
BtsI GCAGTG_nn'
Cuts at: 2515 3239 3541 4335 4355 2515
Size: 724 302 794 20 4185
Fragments arranged by size:
4185 794 724 302 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2099 2134 529
Size: 35 1535 35 4420
Fragments arranged by size:
4420 1535 35 35
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DraIII CAC_nnn'GTG
Cuts at: 3250 3250
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5032 809
Size: 507 3716 1802
Fragments arranged by size:
3716 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2685 3024 3156
Size: 54 1425 50 275 18 855 339 132
Cuts at: 3156 3472 4361 5990 8
Size: 316 889 1629 43
Fragments arranged by size:
1629 1425 889 855 339 316 275 132
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3472 1537
Size: 275 1660 4090
Fragments arranged by size:
4090 1660 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2242 2398 2606 3955 1353
Size: 765 124 156 208 1349 3423
Fragments arranged by size:
3423 1349 765 208 156 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4612 4986 5132 387
Size: 1064 138 3023 374 146 1280
Fragments arranged by size:
3023 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2137 2444 2753 2784 3215 3244
Size: 393 468 37 307 309 31 431 29
Cuts at: 3244 4088 4685 5472 5740 1239
Size: 844 597 787 268 1524
Fragments arranged by size:
1524 844 787 597 468 431 393 309
307 268 37 31 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2353 2353
Size: 6025
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 3022 5172 1678
Size: 1344 2150 2531
Fragments arranged by size:
2531 2150 1344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 4666 4698 4740
Size: 32 135 32 752 32 2310 32 42
Cuts at: 4740 4772 1373
Size: 32 2626
Fragments arranged by size:
2626 2310 752 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3615 669
Size: 988 281 1431 246 3079
Fragments arranged by size:
3079 1431 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6025
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KpnI G_GTAC'C
Cuts at: 3350 3350
Size: 6025
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 2601 5139 5323 1305
Size: 1116 180 2538 184 2007
Fragments arranged by size:
2538 2007 1116 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2687 3026 10
Size: 54 1425 343 855 339 3009
Fragments arranged by size:
3009 1425 855 343 339 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2537 2788 3228 3983 4342 4501 518
Size: 648 1371 251 440 755 359 159 2042
Fragments arranged by size:
2042 1371 755 648 440 359 251 159
MspA1I CmG'CkG
Cuts at: 1606 2029 3112 3193 3441 4118 5266 5511 1606
Size: 423 1083 81 248 677 1148 245 2120
Fragments arranged by size:
2120 1148 1083 677 423 248 245 81
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5473
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 2542 3383 4928 1132
Size: 97 1313 841 1545 2229
Fragments arranged by size:
2229 1545 1313 841 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6025
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2889 3148 1108
Size: 1781 259 3985
Fragments arranged by size:
3985 1781 259
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3015 3595 3015
Size: 580 5445
PstI C_TGCA'G
Cuts at: 830 2034 2145 3311 3377 830
Size: 1204 111 1166 66 3478
Fragments arranged by size:
3478 1204 1166 111 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 3112 3193 3112
Size: 81 5944
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SapI GCTCTTCn'nnn_
Cuts at: 2118 2606 2118
Size: 488 5537
SbfI CC_TGCA'GG
Cuts at: 2145 3311 3377 2145
Size: 1166 66 4793
Fragments arranged by size:
4793 1166 66
ScaI AGT'ACT
Cuts at: 2675 4273 5985 2675
Size: 1598 1712 2715
Fragments arranged by size:
2715 1712 1598
SfcI C'TryA_G
Cuts at: 826 1045 2030 2141 2452 3307 3373 4508 5189
Size: 219 985 111 311 855 66 1135 681
Cuts at: 5189 5380 826
Size: 191 1471
Fragments arranged by size:
1471 1135 985 855 681 311 219 191
111 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 3352 1872
Size: 1480 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4129 5030 5292 820
Size: 197 926 2186 901 262 1553
Fragments arranged by size:
2186 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 3383 1132
Size: 2251 3774
SspI AAT'ATT
Cuts at: 5 52 3949 5
Size: 47 3897 2081
Fragments arranged by size:
3897 2081 47
StuI AGG'CCT
Cuts at: 1517 2359 2715 1517
Size: 842 356 4827
Fragments arranged by size:
4827 842 356
StyI C'CwwG_G
Cuts at: 513 1065 2485 3130 3273 3464 513
Size: 552 1420 645 143 191 3074
Fragments arranged by size:
3074 1420 645 552 191 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2844 4052 4069 4222 4407 4852 325
Size: 1255 1264 1208 17 153 185 445 1498
Fragments arranged by size:
1498 1264 1255 1208 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 2673 4271
Size: 274 80 33 51 1443 462 234 1598
Cuts at: 4271 5983 96
Size: 1712 138
Fragments arranged by size:
1712 1598 1443 462 274 234 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2884 2916 3138 3170 5985 6017 2884
Size: 32 222 32 2815 32 2892
Fragments arranged by size:
2892 2815 222 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6025
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2362 2839 2362
Size: 477 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 3350 1870
Size: 1480 4545
XmnI GAAnn'nnTTC
Cuts at: 2305 4154 2305
Size: 1849 4176
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI