Restriction map for
pFN21AB6629
(Circular) (Six-base) MAPSORT of: pFN21AB6629.seq Check: 4167 from: 1 to: 5950
pFN21AB6629 5950 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3293 1937
Size: 1356 4594
Acc65I G'GTAC_C
Cuts at: 2028 3271 2028
Size: 1243 4707
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2672 3215 4013 5397 5665 1632
Size: 1040 543 798 1384 268 1917
Fragments arranged by size:
1917 1384 1040 798 543 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 2862 4849 5826 1203
Size: 1659 1987 977 1327
Fragments arranged by size:
1987 1659 1327 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 2593 4679 2593
Size: 2086 3864
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AlwNI CAG_nnn'CTG
Cuts at: 2650 3693 5265 2650
Size: 1043 1572 3335
Fragments arranged by size:
3335 1572 1043
ApaI G_GGCC'C
Cuts at: 3136 3136
Size: 5950
ApaLI G'TGCA_C
Cuts at: 3242 4007 5163 3242
Size: 765 1156 4029
Fragments arranged by size:
4029 1156 765
ApoI r'AATT_y
Cuts at: 1493 2300 2744 2761 2885 3259 3485 3637 5746
Size: 807 444 17 124 374 226 152 2109
Cuts at: 5746 1493
Size: 1697
Fragments arranged by size:
2109 1697 807 444 374 226 152 124
17
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 3275 1870
Size: 73 1332 4545
Fragments arranged by size:
4545 1332 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 2891 3271 4726 618
Size: 325 217 627 241 863 380 1455 1842
Fragments arranged by size:
1842 1455 863 627 380 325 241 217
BanII G_rGCy'C
Cuts at: 1391 2633 3136 3269 1391
Size: 1242 503 133 4072
Fragments arranged by size:
4072 1242 503 133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3221 928
Size: 971 1322 3657
Fragments arranged by size:
3657 1322 971
BbvCI CC'TCA_GC
Cuts at: 2145 2145
Size: 5950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4141 4175 1903
Size: 34 2204 34 3678
Fragments arranged by size:
3678 2204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 2340 1548
Size: 792 5158
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2900 4561 136
Size: 107 122 71 631 605 1228 1661 1525
Fragments arranged by size:
1661 1525 1228 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 3136 3246 4011 5167 1790
Size: 1346 110 765 1156 2573
Fragments arranged by size:
2573 1346 1156 765 110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2278 2291 4610 1239
Size: 1039 13 2319 2579
Fragments arranged by size:
2579 2319 1039 13
Bpu10I CC'TnA_GC
Cuts at: 2145 2145
Size: 5950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4613 882
Size: 617 3114 2219
Fragments arranged by size:
3114 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2608 2863 3741 493
Size: 711 1404 255 878 2702
Fragments arranged by size:
2702 1404 878 711 255
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2913 3757 4139 4774
Size: 53 83 186 936 1380 844 382 635
Cuts at: 4774 275
Size: 1451
Fragments arranged by size:
1451 1380 936 844 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2823 4383 4781 5055 5202 5613 2823
Size: 1560 398 274 147 411 3160
Fragments arranged by size:
3160 1560 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2091 2121 2276 2306 1178
Size: 30 165 30 688 30 155 30 4822
Fragments arranged by size:
4822 688 165 155 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2202 2954 3139 1186
Size: 1016 752 185 3997
Fragments arranged by size:
3997 1016 752 185
BseYI C'CCAG_C
Cuts at: 2035 2257 5153 5649 2035
Size: 222 2896 496 2336
Fragments arranged by size:
2896 2336 496 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3107 1163
Size: 573 1371 4006
Fragments arranged by size:
4006 1371 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3400 4161 5189 1540
Size: 275 186 1000 399 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 399 275 186
BsiHKAI G_wGCw'C
Cuts at: 3246 4011 4096 5167 3246
Size: 765 85 1071 4029
Fragments arranged by size:
4029 1071 765 85
BsmI GAATG_Cn'
Cuts at: 3461 3554 3461
Size: 93 5857
BsmBI CGTCTCn'nnnn_
Cuts at: 3088 3088
Size: 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2633 3136 3246 3269 4011 4096 5167
Size: 399 843 503 110 23 742 85 1071
Cuts at: 5167 1391
Size: 2174
Fragments arranged by size:
2174 1071 843 742 503 399 110 85
23
BspEI T'CCGG_A
Cuts at: 2823 5613 2823
Size: 2790 3160
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2817 3305 844
Size: 1973 488 3489
Fragments arranged by size:
3489 1973 488
BsrBI CCG'CTC
Cuts at: 1394 1823 2920 2944 3103 3837 1394
Size: 429 1097 24 159 734 3507
Fragments arranged by size:
3507 1097 734 429 159 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 2088 4445 4619 65
Size: 1183 840 2357 174 1396
Fragments arranged by size:
2357 1396 1183 840 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 4594 4781 1700
Size: 110 221 2563 187 2869
Fragments arranged by size:
2869 2563 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 2439 96
Size: 2343 3607
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945
Size: 1308 367 385 17 1452 86 12 346
Cuts at: 5945 1972
Size: 1977
Fragments arranged by size:
1977 1452 1308 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2974 3127 513
Size: 552 939 970 153 3336
Fragments arranged by size:
3336 970 939 552 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2822 487
Size: 683 56 1596 3615
Fragments arranged by size:
3615 1596 683 56
BtsI GCAGTG_nn'
Cuts at: 3466 4260 4280 3466
Size: 794 20 5136
Fragments arranged by size:
5136 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2237 4957 809
Size: 507 921 2720 1802
Fragments arranged by size:
2720 1802 921 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2460 2998 3397
Size: 54 1425 50 275 18 630 538 399
Cuts at: 3397 4286 5915 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 630 538 399 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 3397 1537
Size: 275 1186 399 4090
Fragments arranged by size:
4090 1186 399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 2285 2575 3880 1353
Size: 744 188 290 1305 3423
Fragments arranged by size:
3423 1305 744 290 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4537 4911 5057 387
Size: 1064 138 2948 374 146 1280
Fragments arranged by size:
2948 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2278 2291 2672 3215 4013 4610 5397
Size: 393 646 13 381 543 798 597 787
Cuts at: 5397 5665 1239
Size: 268 1524
Fragments arranged by size:
1524 798 787 646 597 543 393 381
268 13
EcoO109I rG'GnC_Cy
Cuts at: 2275 3132 3133 2275
Size: 857 1 5092
Fragments arranged by size:
5092 857 1
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 5097 1678
Size: 3419 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 2579 2611 3193
Size: 32 135 32 609 32 366 32 582
Cuts at: 3193 3225 4591 4623 4665 4697 1373
Size: 32 1366 32 42 32 2626
Fragments arranged by size:
2626 1366 609 582 366 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3142 3294 3540 669
Size: 988 281 1204 152 246 3079
Fragments arranged by size:
3079 1204 988 281 246 152
HindIII A'AGCT_T
Cuts at: 748 2117 748
Size: 1369 4581
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 2032 3275 2032
Size: 1243 4707
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3180 5064 5248 1305
Size: 1875 1884 184 2007
Fragments arranged by size:
2007 1884 1875 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2462 10
Size: 54 1425 343 630 3498
Fragments arranged by size:
3498 1425 630 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2385 2717 3908 4267 4426 518
Size: 648 1080 139 332 1191 359 159 2042
Fragments arranged by size:
2042 1191 1080 648 359 332 159 139
MspA1I CmG'CkG
Cuts at: 1606 2055 2976 3129 3366 4043 5191 5436 1606
Size: 449 921 153 237 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 677 449 245 237 153
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4459
Fragments arranged by size:
4459 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2881 3190 3308 4853 1132
Size: 97 1652 309 118 1545 2229
Fragments arranged by size:
2229 1652 1545 309 118 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2211 1108
Size: 1103 4847
PfoI T'CCnGG_A
Cuts at: 2294 2294
Size: 5950
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2275
Size: 5950
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PspOMI G'GGCC_C
Cuts at: 3132 3132
Size: 5950
PstI C_TGCA'G
Cuts at: 830 2098 3302 830
Size: 1268 1204 3478
Fragments arranged by size:
3478 1268 1204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
SacII CC_GC'GG
Cuts at: 2977 3130 2977
Size: 153 5797
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SexAI A'CCwGG_T
Cuts at: 2433 2433
Size: 5950
SfcI C'TryA_G
Cuts at: 826 1045 2094 3298 4433 5114 5305 826
Size: 219 1049 1204 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 1049 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 2004 2560 3389 513
Size: 552 939 556 829 3074
Fragments arranged by size:
3074 939 829 556 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2501 2949 3977 3994 4147 4332 4777
Size: 1255 921 448 1028 17 153 185 445
Cuts at: 4777 325
Size: 1498
Fragments arranged by size:
1498 1255 1028 921 448 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 2616 4196
Size: 274 80 33 51 1443 462 177 1580
Cuts at: 4196 5908 96
Size: 1712 138
Fragments arranged by size:
1712 1580 1443 462 274 177 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5910 5942 5910
Size: 32 5918
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 2358 2831 4079 2358
Size: 473 1248 4229
Fragments arranged by size:
4229 1248 473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI