(Circular) (Six-base) MAPSORT of: pFN21AB6628.seq Check: 7341 from: 1 to: 5923 pFN21AB6628 5923 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2981 2981 Size: 5923 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2278 2896 3266 1937 Size: 341 618 370 4594 Fragments arranged by size: 4594 618 370 341 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2748 3986 5370 5638 1632 Size: 1116 1238 1384 268 1917 Fragments arranged by size: 1917 1384 1238 1116 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2439 1184 Size: 1255 4668 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3161 3193 3161 Size: 32 5891 AlwNI CAG_nnn'CTG Cuts at: 2974 3666 5238 2974 Size: 692 1572 3659 Fragments arranged by size: 3659 1572 692 ApaI G_GGCC'C Cuts at: 2863 3130 2863 Size: 267 5656 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2430 2540 3232 3458 3610 5719 1493 Size: 937 110 692 226 152 2109 1697 Fragments arranged by size: 2109 1697 937 692 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2439 3248 1870 Size: 73 496 809 4545 Fragments arranged by size: 4545 809 496 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3244 4699 618 Size: 325 217 627 1457 1455 1842 Fragments arranged by size: 1842 1457 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2757 2863 3130 3242 1391 Size: 1366 106 267 112 4072 Fragments arranged by size: 4072 1366 267 112 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2736 928 Size: 971 837 4115 Fragments arranged by size: 4115 971 837 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2149 2183 4114 4148 1903 Size: 34 212 34 1931 34 3678 Fragments arranged by size: 3678 1931 212 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2858 3086 4534 Size: 107 122 71 631 605 1186 228 1448 Cuts at: 4534 136 Size: 1525 Fragments arranged by size: 1525 1448 1186 631 605 228 122 107 71 BglII A'GATC_T Cuts at: 2443 5918 2443 Size: 3475 2448 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 2854 2863 3130 3984 5140 1790 Size: 1064 9 267 854 1156 2573 Fragments arranged by size: 2573 1156 1064 854 267 9 BmgBI CAC'GTC Cuts at: 1318 2513 1318 Size: 1195 4728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2364 2592 3175 Size: 416 53 83 399 24 947 228 583 Cuts at: 3175 3654 4612 442 Size: 479 958 1753 Fragments arranged by size: 1753 958 947 583 479 416 399 228 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 3181 4583 1239 Size: 1229 713 1402 2579 Fragments arranged by size: 2579 1402 1229 713 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2141 4586 882 Size: 617 642 2445 2219 Fragments arranged by size: 2445 2219 642 617 BsaAI yAC'GTr Cuts at: 493 1204 2489 2536 3714 493 Size: 711 1285 47 1178 2702 Fragments arranged by size: 2702 1285 1178 711 47 BsaBI GATnn'nnATC Cuts at: 2199 5917 2199 Size: 3718 2205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3730 4112 4747 275 Size: 53 83 186 936 2197 382 635 1451 Fragments arranged by size: 2197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2206 2218 2236 2248 3161 Size: 30 165 30 803 12 18 12 913 Cuts at: 3161 3191 1178 Size: 30 3910 Fragments arranged by size: 3910 913 803 165 30 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 1186 Size: 1213 4710 BseYI C'CCAG_C Cuts at: 2268 2596 5126 5622 2268 Size: 328 2530 496 2569 Fragments arranged by size: 2569 2530 496 328 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2696 1163 Size: 573 960 4390 Fragments arranged by size: 4390 960 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3984 4069 5140 3984 Size: 85 1071 4767 Fragments arranged by size: 4767 1071 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2757 2854 2863 3130 3242 3984 4069 Size: 399 967 97 9 267 112 742 85 Cuts at: 4069 5140 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 967 742 399 267 112 97 85 9 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2673 2981 3278 844 Size: 1829 308 297 3489 Fragments arranged by size: 3489 1829 308 297 BsrBI CCG'CTC Cuts at: 1394 1823 2808 3810 1394 Size: 429 985 1002 3507 Fragments arranged by size: 3507 1002 985 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2149 2526 2683 4418 4592 65 Size: 1183 901 377 157 1735 174 1396 Fragments arranged by size: 1735 1396 1183 901 377 174 157 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 2614 96 Size: 2518 3405 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 2593 794 Size: 1799 4124 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2443 2977 3253 3620 4005 4022 5474 5560 Size: 471 534 276 367 385 17 1452 86 Cuts at: 5560 5572 5918 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 534 471 385 367 346 276 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2382 2382 Size: 5923 BtgI C'CryG_G Cuts at: 513 1065 2004 2652 3219 513 Size: 552 939 648 567 3217 Fragments arranged by size: 3217 939 648 567 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2496 487 Size: 683 56 1270 3914 Fragments arranged by size: 3914 1270 683 56 BtsI GCAGTG_nn' Cuts at: 2252 3051 3439 4233 4253 2252 Size: 799 388 794 20 3922 Fragments arranged by size: 3922 799 794 388 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2568 2655 2911 Size: 54 1425 50 275 18 738 87 256 Cuts at: 2911 3370 4259 5888 8 Size: 459 889 1629 43 Fragments arranged by size: 1629 1425 889 738 459 275 256 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2061 3853 1353 Size: 708 1792 3423 Fragments arranged by size: 3423 1792 708 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 2748 3181 3986 4583 5370 5638 Size: 393 836 280 433 805 597 787 268 Cuts at: 5638 1239 Size: 1524 Fragments arranged by size: 1524 836 805 787 597 433 393 280 268 EcoNI CCTnn'n_nnAGG Cuts at: 2669 2786 2669 Size: 117 5806 EcoO109I rG'GnC_Cy Cuts at: 2860 3126 3127 2860 Size: 266 1 5656 Fragments arranged by size: 5656 266 1 EcoRI G'AATT_C Cuts at: 2430 2540 3232 3610 2430 Size: 110 692 378 4743 Fragments arranged by size: 4743 692 378 110 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 3033 5070 1678 Size: 1355 2037 2531 Fragments arranged by size: 2531 2037 1355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2167 2199 2206 2218 2238 Size: 32 135 32 595 32 7 12 20 Cuts at: 2238 2250 2444 2476 2492 2524 2592 2624 2969 Size: 12 194 32 16 32 68 32 345 Cuts at: 2969 3001 4564 4596 4638 4670 1373 Size: 32 1563 32 42 32 2626 Fragments arranged by size: 2626 1563 595 345 194 135 68 42 32 32 32 32 32 32 32 32 32 20 16 12 12 7 HincII GTy'rAC Cuts at: 669 1657 1938 2279 2897 3267 3513 669 Size: 988 281 341 618 370 246 3079 Fragments arranged by size: 3079 988 618 370 341 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2204 3064 5037 5221 1305 Size: 899 860 1973 184 2007 Fragments arranged by size: 2007 1973 899 860 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2570 2657 2913 10 Size: 54 1425 343 738 87 256 3020 Fragments arranged by size: 3020 1425 738 343 256 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2286 2313 3881 4240 4399 518 Size: 648 1120 27 1568 359 159 2042 Fragments arranged by size: 2042 1568 1120 648 359 159 27 MspA1I CmG'CkG Cuts at: 1606 2590 3339 4016 5164 5409 1606 Size: 984 749 677 1148 245 2120 Fragments arranged by size: 2120 1148 984 749 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2652 3219 513 Size: 552 939 648 567 3217 Fragments arranged by size: 3217 939 648 567 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NruI TCG'CGA Cuts at: 2296 2481 2296 Size: 185 5738 NspI r_CATG'y Cuts at: 1132 1229 2737 3281 4826 1132 Size: 97 1508 544 1545 2229 Fragments arranged by size: 2229 1545 1508 544 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2974 3220 1108 Size: 1866 246 3811 Fragments arranged by size: 3811 1866 246 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3161 3193 3992 4024 5505 5537 3161 Size: 32 799 32 1481 32 3547 Fragments arranged by size: 3547 1481 799 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2310 1613 Size: 697 5226 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 2859 3126 2859 Size: 267 5656 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2460 2492 2460 Size: 32 5891 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 SalI G'TCGA_C Cuts at: 1936 2277 2895 3265 1936 Size: 341 618 370 4594 Fragments arranged by size: 4594 618 370 341 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 2466 3271 4406 5087 5278 826 Size: 219 1421 805 1135 681 191 1471 Fragments arranged by size: 1471 1421 1135 805 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2439 4027 4928 5190 820 Size: 197 926 496 1588 901 262 1553 Fragments arranged by size: 1588 1553 926 901 496 262 197 SnaBI TAC'GTA Cuts at: 493 2489 3714 493 Size: 1996 1225 2702 Fragments arranged by size: 2702 1996 1225 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 2776 3847 5 Size: 47 2724 1071 2081 Fragments arranged by size: 2724 2081 1071 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2004 2652 3120 3219 3362 513 Size: 552 939 648 468 99 143 3074 Fragments arranged by size: 3074 939 648 552 468 143 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3950 3967 4120 4305 4750 325 Size: 1255 2370 17 153 185 445 1498 Fragments arranged by size: 2370 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2614 4169 5881 Size: 274 80 33 51 1443 637 1555 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1712 1555 1443 637 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 2439 1943 Size: 496 5427 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI