Restriction map for
pFN21AB6625
(Circular) (Six-base) MAPSORT of: pFN21AB6625.seq Check: 1314 from: 1 to: 5923
pFN21AB6625 5923 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2401 2401
Size: 5923
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2874 3733 278
Size: 53 83 186 936 1338 859 2468
Fragments arranged by size:
2468 1338 936 859 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 2064 4050 4423 1563
Size: 501 1986 373 3063
Fragments arranged by size:
3063 1986 501 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3986 5370 5638 1632
Size: 2354 1384 268 1917
Fragments arranged by size:
2354 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 3666 5238 3666
Size: 1572 4351
ApaI G_GGCC'C
Cuts at: 2539 2906 2539
Size: 367 5556
ApaLI G'TGCA_C
Cuts at: 2441 3980 5136 2441
Size: 1539 1156 3228
Fragments arranged by size:
3228 1539 1156
ApoI r'AATT_y
Cuts at: 1493 3232 3458 3610 5719 1493
Size: 1739 226 152 2109 1697
Fragments arranged by size:
2109 1739 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2140 2629 3034 3248 1870
Size: 73 197 489 405 214 4545
Fragments arranged by size:
4545 489 405 214 197 73
BamHI G'GATC_C
Cuts at: 2702 3253 3620 2702
Size: 551 367 5005
Fragments arranged by size:
5005 551 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3161 3244 4699 618
Size: 325 217 627 1374 83 1455 1842
Fragments arranged by size:
1842 1455 1374 627 325 217 83
BanII G_rGCy'C
Cuts at: 1391 2539 2906 3015 3086 3110 3242 1391
Size: 1148 367 109 71 24 132 4072
Fragments arranged by size:
4072 1148 367 132 109 71 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2505 928
Size: 971 606 4346
Fragments arranged by size:
4346 971 606
BbvCI CC'TCA_GC
Cuts at: 2171 2415 2171
Size: 244 5679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4534 136
Size: 107 122 71 631 605 2862 1525
Fragments arranged by size:
2862 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 3009 3340 3009
Size: 331 5592
Bme1580I G_kGCm'C
Cuts at: 1790 2445 2539 2906 3164 3192 3984 5140 1790
Size: 655 94 367 258 28 792 1156 2573
Fragments arranged by size:
2573 1156 792 655 367 258 94 28
BmgBI CAC'GTC
Cuts at: 1318 3054 1318
Size: 1736 4187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3229 3654 4612
Size: 416 53 83 399 24 1812 425 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1812 1753 958 425 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2285 2402 2656 2673 2794 4583 1239
Size: 914 132 117 254 17 121 1789 2579
Fragments arranged by size:
2579 1789 914 254 132 121 117 17
Bpu10I CC'TnA_GC
Cuts at: 2171 2415 2171
Size: 244 5679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 2744 4048 4913 5211 2240
Size: 504 1304 865 298 2952
Fragments arranged by size:
2952 1304 865 504 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2382 2824 2987 4586 882
Size: 617 883 442 163 1599 2219
Fragments arranged by size:
2219 1599 883 617 442 163
BsaAI yAC'GTr
Cuts at: 493 1204 2554 3714 493
Size: 711 1350 1160 2702
Fragments arranged by size:
2702 1350 1160 711
BsaBI GATnn'nnATC
Cuts at: 2283 5917 2283
Size: 3634 2289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2723 2831 2871 3730
Size: 53 83 186 936 1190 108 40 859
Cuts at: 3730 4112 4747 275
Size: 382 635 1451
Fragments arranged by size:
1451 1190 936 859 635 382 186 108
83 53 40
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 1178
Size: 30 165 30 1104 30 4564
Fragments arranged by size:
4564 1104 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2470 3091 3094 1186
Size: 1284 621 3 4015
Fragments arranged by size:
4015 1284 621 3
BseYI C'CCAG_C
Cuts at: 2731 3093 3204 5126 5622 2731
Size: 362 111 1922 496 3032
Fragments arranged by size:
3032 1922 496 362 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 1163
Size: 573 333 5017
Fragments arranged by size:
5017 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3186 3373 4134 5162 1540
Size: 275 186 1185 187 761 1028 2301
Fragments arranged by size:
2301 1185 1028 761 275 187 186
BsiHKAI G_wGCw'C
Cuts at: 2141 2445 3086 3984 4069 5140 2141
Size: 304 641 898 85 1071 2924
Fragments arranged by size:
2924 1071 898 641 304 85
BsmI GAATG_Cn'
Cuts at: 2248 3434 3527 2248
Size: 1186 93 4644
Fragments arranged by size:
4644 1186 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2445 2539 2906 3015 3086 3110
Size: 399 351 304 94 367 109 71 24
Cuts at: 3110 3164 3192 3242 3984 4069 5140 1391
Size: 54 28 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 351 304 109
94 85 71 54 50 28 24
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2401 3278 844
Size: 1557 877 3489
Fragments arranged by size:
3489 1557 877
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2661 3181 4567 4754 1700
Size: 110 851 520 1386 187 2869
Fragments arranged by size:
2869 1386 851 520 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2124 2702 3253 3620 4005 4022 5474 5560
Size: 152 578 551 367 385 17 1452 86
Cuts at: 5560 5572 5918 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 578 551 385 367 346 152
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2152 2325 2436 513
Size: 552 939 148 173 111 4000
Fragments arranged by size:
4000 939 552 173 148 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 3180 3439 4233 4253 3180
Size: 259 794 20 4850
Fragments arranged by size:
4850 794 259 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2431 3028 4930 809
Size: 507 1115 597 1902 1802
Fragments arranged by size:
1902 1802 1115 597 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2358 2455
Size: 54 1425 50 275 18 177 351 97
Cuts at: 2455 3183 3370 4259 5888 8
Size: 728 187 889 1629 43
Fragments arranged by size:
1629 1425 889 728 351 275 187 177
97 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3183 3370 1537
Size: 275 1371 187 4090
Fragments arranged by size:
4090 1371 275 187
EarI CTCTTCn'nnn_
Cuts at: 1353 3853 1353
Size: 2500 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3084 3084
Size: 5923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2285 2402 2656 2673 2794 3986
Size: 393 521 132 117 254 17 121 1192
Cuts at: 3986 4583 5370 5638 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1192 787 597 521 393 268 254
132 121 117 17
EcoO109I rG'GnC_Cy
Cuts at: 2535 2902 2903 2535
Size: 367 1 5555
Fragments arranged by size:
5555 367 1
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 2769 4429 2769
Size: 1660 4263
HaeII r_GCGC'y
Cuts at: 1678 2691 5070 1678
Size: 1013 2379 2531
Fragments arranged by size:
2531 2379 1013
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 2507 2539 2792
Size: 32 135 32 826 32 77 32 253
Cuts at: 2792 2824 2903 2935 4564 4596 4638 4670 1373
Size: 32 79 32 1629 32 42 32 2626
Fragments arranged by size:
2626 1629 826 253 135 79 77 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2457 10
Size: 54 1425 343 177 448 3476
Fragments arranged by size:
3476 1425 448 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3881 4240 4399 518
Size: 648 2715 359 159 2042
Fragments arranged by size:
2715 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2154 2327 2946 3048 3093 3339 4016 5164
Size: 548 173 619 102 45 246 677 1148
Cuts at: 5164 5409 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 619 548 246 245 173
102 45
NaeI GCC'GGC
Cuts at: 3183 3183
Size: 5923
NcoI C'CATG_G
Cuts at: 513 1065 2004 2436 513
Size: 552 939 432 4000
Fragments arranged by size:
4000 939 552 432
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NgoMIV G'CCGG_C
Cuts at: 3181 3181
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3281 4826 1132
Size: 97 2052 1545 2229
Fragments arranged by size:
2229 2052 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2539 1348
Size: 1191 4732
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5923
PfoI T'CCnGG_A
Cuts at: 2262 2262
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspOMI G'GGCC_C
Cuts at: 2535 2902 2535
Size: 367 5556
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 3048 3093 3048
Size: 45 5878
SacI G_AGCT'C
Cuts at: 3086 3086
Size: 5923
SacII CC_GC'GG
Cuts at: 2155 2328 2155
Size: 173 5750
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 3271 4406 5087 5278 826
Size: 219 2226 1135 681 191 1471
Fragments arranged by size:
2226 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2219 2759 4027 4928 5190 820
Size: 197 926 276 540 1268 901 262 1553
Fragments arranged by size:
1553 1268 926 901 540 276 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2436 2679 2835 2907 3362 513
Size: 552 939 432 243 156 72 455 3074
Fragments arranged by size:
3074 939 552 455 432 243 156 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3950 3967 4120 4305 4750 325
Size: 1255 2370 17 153 185 445 1498
Fragments arranged by size:
2370 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 2625 4169
Size: 274 80 33 51 1443 333 315 1544
Cuts at: 4169 5881 96
Size: 1712 138
Fragments arranged by size:
1712 1544 1443 333 315 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2872 3731 276
Size: 53 83 186 936 1338 859 2468
Fragments arranged by size:
2468 1338 936 859 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI