Restriction map for
pFN21AB6623
(Circular) (Six-base) MAPSORT of: pFN21AB6623.seq Check: 8973 from: 1 to: 5944
pFN21AB6623 5944 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3754 278
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3287 1937
Size: 1350 4594
Acc65I G'GTAC_C
Cuts at: 3265 3265
Size: 5944
AclI AA'CG_TT
Cuts at: 1563 4071 4444 1563
Size: 2508 373 3063
Fragments arranged by size:
3063 2508 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2133 2318 4007 5391 5659 1632
Size: 501 185 1689 1384 268 1917
Fragments arranged by size:
1917 1689 1384 501 268 185
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5747
AflIII A'CryG_T
Cuts at: 1203 4843 5820 1203
Size: 3640 977 1327
Fragments arranged by size:
3640 1327 977
AgeI A'CCGG_T
Cuts at: 4775 4775
Size: 5944
AhdI GACnn_n'nnGTC
Cuts at: 4673 4673
Size: 5944
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5944
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5944
AlwNI CAG_nnn'CTG
Cuts at: 3687 5259 3687
Size: 1572 4372
ApaLI G'TGCA_C
Cuts at: 4001 5157 4001
Size: 1156 4788
ApoI r'AATT_y
Cuts at: 1493 2080 2789 3253 3479 3631 5740 1493
Size: 587 709 464 226 152 2109 1697
Fragments arranged by size:
2109 1697 709 587 464 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4498 160
Size: 872 3466 1606
Fragments arranged by size:
3466 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
AvaI C'yCGr_G
Cuts at: 1870 1943 3269 1870
Size: 73 1326 4545
Fragments arranged by size:
4545 1326 73
BamHI G'GATC_C
Cuts at: 3274 3641 3274
Size: 367 5577
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3265 4720 618
Size: 325 217 627 1478 1455 1842
Fragments arranged by size:
1842 1478 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2050 2148 2351 3263 1391
Size: 659 98 203 912 4072
Fragments arranged by size:
4072 912 659 203 98
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2336 2603 2750 2832 3049 3165 928
Size: 971 437 267 147 82 217 116 3707
Fragments arranged by size:
3707 971 437 267 217 147 116 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4135 4169 1903
Size: 34 2198 34 3678
Fragments arranged by size:
3678 2198 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2048 2167 3836 5046 2048
Size: 119 1669 1210 2946
Fragments arranged by size:
2946 1669 1210 119
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5944
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2231 4555 136
Size: 107 122 71 631 605 559 2324 1525
Fragments arranged by size:
2324 1525 631 605 559 122 107 71
BglII A'GATC_T
Cuts at: 5939 5939
Size: 5944
BlpI GC'TnA_GC
Cuts at: 3361 3361
Size: 5944
Bme1580I G_kGCm'C
Cuts at: 1790 3068 4005 5161 1790
Size: 1278 937 1156 2573
Fragments arranged by size:
2573 1278 1156 937
BmgBI CAC'GTC
Cuts at: 1318 2170 1318
Size: 852 5092
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2209 3675 4633
Size: 416 53 83 399 24 792 1466 958
Cuts at: 4633 442
Size: 1753
Fragments arranged by size:
1753 1466 958 792 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5944
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3189 3189
Size: 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2804 4604 1239
Size: 1565 1800 2579
Fragments arranged by size:
2579 1800 1565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2882 2906 4069 4934 5232 2882
Size: 24 1163 865 298 3594
Fragments arranged by size:
3594 1163 865 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4607 882
Size: 617 3108 2219
Fragments arranged by size:
3108 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3735 493
Size: 711 2531 2702
Fragments arranged by size:
2702 2531 711
BsaBI GATnn'nnATC
Cuts at: 5938 5938
Size: 5944
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3751 4133 4768 275
Size: 53 83 186 936 2218 382 635 1451
Fragments arranged by size:
2218 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3204 4377 4775 5049 5196 5607 3204
Size: 1173 398 274 147 411 3541
Fragments arranged by size:
3541 1173 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5719
Fragments arranged by size:
5719 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3122 1186
Size: 1936 4008
BseYI C'CCAG_C
Cuts at: 5147 5643 5147
Size: 496 5448
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5371
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3394 4155 5183 1540
Size: 275 186 1393 761 1028 2301
Fragments arranged by size:
2301 1393 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4005 4090 5161 4005
Size: 85 1071 4788
Fragments arranged by size:
4788 1071 85
BsmI GAATG_Cn'
Cuts at: 3455 3548 3455
Size: 93 5851
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2148 2351 3068 3263 4005 4090
Size: 399 260 98 203 717 195 742 85
Cuts at: 4090 5161 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 717 399 260 203 195
98 85
BspEI T'CCGG_A
Cuts at: 3204 5607 3204
Size: 2403 3541
BspHI T'CATG_A
Cuts at: 3833 5563 3833
Size: 1730 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2616 3299 844
Size: 1772 683 3489
Fragments arranged by size:
3489 1772 683
BsrBI CCG'CTC
Cuts at: 1394 1823 3831 1394
Size: 429 2008 3507
Fragments arranged by size:
3507 2008 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2517 4439 4613 65
Size: 1183 1269 1922 174 1396
Fragments arranged by size:
1922 1396 1269 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4588 4775 1700
Size: 110 2778 187 2869
Fragments arranged by size:
2869 2778 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5944
BssSI C'ACGA_G
Cuts at: 4004 5016 4004
Size: 1012 4932
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5944
BstBI TT'CG_AA
Cuts at: 4748 4748
Size: 5944
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5944
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 2530 2341
Size: 189 5755
BstYI r'GATC_y
Cuts at: 1972 3274 3641 4026 4043 5495 5581 5593 5939
Size: 1302 367 385 17 1452 86 12 346
Cuts at: 5939 1972
Size: 1977
Fragments arranged by size:
1977 1452 1302 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3021 513
Size: 552 1956 3436
Fragments arranged by size:
3436 1956 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5205
Fragments arranged by size:
5205 683 56
BtsI GCAGTG_nn'
Cuts at: 3460 4254 4274 3460
Size: 794 20 5130
Fragments arranged by size:
5130 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5944
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5909
DraI TTT'AAA
Cuts at: 3248 3248
Size: 5944
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4951 809
Size: 507 3635 1802
Fragments arranged by size:
3635 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 3391 4280
Size: 54 1425 50 275 18 393 1168 889
Cuts at: 4280 5909 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1168 889 393 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3391 1537
Size: 275 1579 4090
Fragments arranged by size:
4090 1579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2901 3874 1353
Size: 1548 973 3423
Fragments arranged by size:
3423 1548 973
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4531 4905 5051 387
Size: 1064 138 2942 374 146 1280
Fragments arranged by size:
2942 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2318 2804 4007 4604 5391 5659
Size: 393 501 185 486 1203 597 787 268
Cuts at: 5659 1239
Size: 1524
Fragments arranged by size:
1524 1203 787 597 501 486 393 268
185
EcoRI G'AATT_C
Cuts at: 3253 3631 3253
Size: 378 5566
FspI TGC'GCA
Cuts at: 4450 4450
Size: 5944
HaeII r_GCGC'y
Cuts at: 1678 2917 5091 1678
Size: 1239 2174 2531
Fragments arranged by size:
2531 2174 1239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2957 2989 4585
Size: 32 135 32 809 32 544 32 1596
Cuts at: 4585 4617 4659 4691 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1596 809 544 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2374 3288 3534 669
Size: 988 281 436 914 246 3079
Fragments arranged by size:
3079 988 914 436 281 246
HindIII A'AGCT_T
Cuts at: 748 2258 748
Size: 1510 4434
HpaI GTT'AAC
Cuts at: 3534 3534
Size: 5944
KpnI G_GTAC'C
Cuts at: 3269 3269
Size: 5944
MfeI C'AATT_G
Cuts at: 2208 3543 2208
Size: 1335 4609
MluI A'CGCG_T
Cuts at: 5820 5820
Size: 5944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2967 3000 5058 5242 1305
Size: 1662 33 2058 184 2007
Fragments arranged by size:
2058 2007 1662 184 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2225 10
Size: 54 1425 343 393 3729
Fragments arranged by size:
3729 1425 393 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 3026 3902 4261 4420 518
Size: 648 870 990 876 359 159 2042
Fragments arranged by size:
2042 990 876 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3360 4037 5185 5430 1606
Size: 1754 677 1148 245 2120
Fragments arranged by size:
2120 1754 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3021 513
Size: 552 1956 3436
Fragments arranged by size:
3436 1956 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5944
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5944
NotI GC'GGCC_GC
Cuts at: 3391 3391
Size: 5944
NspI r_CATG'y
Cuts at: 1132 1229 3302 4847 1132
Size: 97 2073 1545 2229
Fragments arranged by size:
2229 2073 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2050 1348
Size: 702 5242
PciI A'CATG_T
Cuts at: 4843 4843
Size: 5944
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2221 1108
Size: 1113 4831
PfoI T'CCnGG_A
Cuts at: 2958 2958
Size: 5944
PmeI GTTT'AAAC
Cuts at: 3248 3248
Size: 5944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 5526 5558 4013
Size: 32 1481 32 4399
Fragments arranged by size:
4399 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5944
PsiI TTA'TAA
Cuts at: 2319 3514 2319
Size: 1195 4749
PstI C_TGCA'G
Cuts at: 830 2709 3296 830
Size: 1879 587 3478
Fragments arranged by size:
3478 1879 587
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5944
SalI G'TCGA_C
Cuts at: 1936 3286 1936
Size: 1350 4594
SbfI CC_TGCA'GG
Cuts at: 3296 3296
Size: 5944
ScaI AGT'ACT
Cuts at: 4192 5904 4192
Size: 1712 4232
SfcI C'TryA_G
Cuts at: 826 1045 2705 3292 4427 5108 5299 826
Size: 219 1660 587 1135 681 191 1471
Fragments arranged by size:
1660 1471 1135 681 587 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5944
SmaI CCC'GGG
Cuts at: 1872 3271 1872
Size: 1399 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2897 2921 4048 4949 5211 820
Size: 197 926 954 24 1127 901 262 1553
Fragments arranged by size:
1553 1127 954 926 901 262 197 24
SnaBI TAC'GTA
Cuts at: 493 3735 493
Size: 3242 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5944
SphI G_CATG'C
Cuts at: 1132 3302 1132
Size: 2170 3774
SspI AAT'ATT
Cuts at: 5 52 3868 5
Size: 47 3816 2081
Fragments arranged by size:
3816 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5944
StyI C'CwwG_G
Cuts at: 513 1065 2229 2334 3021 3383 513
Size: 552 1164 105 687 362 3074
Fragments arranged by size:
3074 1164 687 552 362 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2107 2202 3971 3988 4141 4326 4771
Size: 1255 527 95 1769 17 153 185 445
Cuts at: 4771 325
Size: 1498
Fragments arranged by size:
1769 1498 1255 527 445 185 153 95
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4190 5902 96
Size: 274 80 33 51 1443 2213 1712 138
Fragments arranged by size:
2213 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5904 5936 5904
Size: 32 5912
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5944
XbaI T'CTAG_A
Cuts at: 3280 3280
Size: 5944
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5944
XmaI C'CCGG_G
Cuts at: 1870 3269 1870
Size: 1399 4545
XmnI GAAnn'nnTTC
Cuts at: 2118 4073 2118
Size: 1955 3989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3752 276
Size: 53 83 186 936 2218 2468
Fragments arranged by size:
2468 2218 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI