Restriction map for
pFN21AB6622
(Circular) (Six-base) MAPSORT of: pFN21AB6622.seq Check: 4920 from: 1 to: 6013
pFN21AB6622 6013 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3356 1937
Size: 1419 4594
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3162 4076 5460 5728 1632
Size: 1530 914 1384 268 1917
Fragments arranged by size:
1917 1530 1384 914 268
AflII C'TTAA_G
Cuts at: 820 1017 2143 820
Size: 197 1126 4690
Fragments arranged by size:
4690 1126 197
AflIII A'CryG_T
Cuts at: 1203 2582 4912 5889 1203
Size: 1379 2330 977 1327
Fragments arranged by size:
2330 1379 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 2145 1166
Size: 979 5034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2846 1184
Size: 1662 4351
AlwNI CAG_nnn'CTG
Cuts at: 3756 5328 3756
Size: 1572 4441
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 2070 2483 2659 3184 3322 3548 3700 5809
Size: 577 413 176 525 138 226 152 2109
Cuts at: 5809 1493
Size: 1697
Fragments arranged by size:
2109 1697 577 525 413 226 176 152
138
AseI AT'TA_AT
Cuts at: 160 1032 3155 4567 160
Size: 872 2123 1412 1606
Fragments arranged by size:
2123 1606 1412 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 2208 3338 1870
Size: 73 265 1130 4545
Fragments arranged by size:
4545 1130 265 73
BamHI G'GATC_C
Cuts at: 3343 3710 3343
Size: 367 5646
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3334 4789 618
Size: 325 217 627 1547 1455 1842
Fragments arranged by size:
1842 1547 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3332 1391
Size: 1941 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5042
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4624 136
Size: 107 122 71 631 605 2952 1525
Fragments arranged by size:
2952 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2905 6008 2905
Size: 3103 2910
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 4074 5230 1790
Size: 2284 1156 2573
Fragments arranged by size:
2573 2284 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6013
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2456 3744 4702
Size: 416 53 83 399 24 1039 1288 958
Cuts at: 4702 442
Size: 1753
Fragments arranged by size:
1753 1288 1039 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4673 1239
Size: 3434 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4138 5003 5301 4138
Size: 865 298 4850
Fragments arranged by size:
4850 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4676 882
Size: 617 3177 2219
Fragments arranged by size:
3177 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2078 3804 493
Size: 711 874 1726 2702
Fragments arranged by size:
2702 1726 874 711
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3820 4202 4837 275
Size: 53 83 186 936 2287 382 635 1451
Fragments arranged by size:
2287 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4446 4844 5118 5265 5676 4446
Size: 398 274 147 411 4783
Fragments arranged by size:
4783 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2112 2142 2370 2400 1178
Size: 30 165 30 709 30 228 30 4791
Fragments arranged by size:
4791 709 228 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6013
BseYI C'CCAG_C
Cuts at: 5216 5712 5216
Size: 496 5517
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2535 1163
Size: 573 799 4641
Fragments arranged by size:
4641 799 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3463 4224 5252 1540
Size: 275 186 1462 761 1028 2301
Fragments arranged by size:
2301 1462 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4074 4159 5230 4074
Size: 85 1071 4857
Fragments arranged by size:
4857 1071 85
BsmI GAATG_Cn'
Cuts at: 3247 3524 3617 3247
Size: 277 93 5643
Fragments arranged by size:
5643 277 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3332 4074 4159 5230 1391
Size: 399 1542 742 85 1071 2174
Fragments arranged by size:
2174 1542 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 3120 3902 5632 3120
Size: 782 1730 3501
Fragments arranged by size:
3501 1730 782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2722 2838 3368 844
Size: 1878 116 530 3489
Fragments arranged by size:
3489 1878 530 116
BsrBI CCG'CTC
Cuts at: 1394 1823 3900 1394
Size: 429 2077 3507
Fragments arranged by size:
3507 2077 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3003 4508 4682 65
Size: 1183 1755 1505 174 1396
Fragments arranged by size:
1755 1505 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2640 4657 4844 1700
Size: 110 830 2017 187 2869
Fragments arranged by size:
2869 2017 830 187 110
BsrGI T'GTAC_A
Cuts at: 96 2956 96
Size: 2860 3153
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6013
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 2441 1184
Size: 1257 4756
BstYI r'GATC_y
Cuts at: 1972 2905 3343 3710 4095 4112 5564 5650 5662
Size: 933 438 367 385 17 1452 86 12
Cuts at: 5662 6008 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 933 438 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2173 3165 513
Size: 552 1108 992 3361
Fragments arranged by size:
3361 1108 992 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2865 487
Size: 683 56 1639 3635
Fragments arranged by size:
3635 1639 683 56
BtsI GCAGTG_nn'
Cuts at: 3290 3529 4323 4343 3290
Size: 239 794 20 4960
Fragments arranged by size:
4960 794 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 2433 2695 2865 3009 3317 2433
Size: 262 170 144 308 5129
Fragments arranged by size:
5129 308 262 170 144
DraIII CAC_nnn'GTG
Cuts at: 2049 2049
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3460 4349 5978
Size: 54 1425 50 275 18 1630 889 1629
Cuts at: 5978 8
Size: 43
Fragments arranged by size:
1630 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3021 3943 1353
Size: 1668 922 3423
Fragments arranged by size:
3423 1668 922
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 4974 5120 387
Size: 1064 138 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3162 4076 4673 5460 5728 1239
Size: 393 1530 914 597 787 268 1524
Fragments arranged by size:
1530 1524 914 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2057
Size: 6013
EcoRI G'AATT_C
Cuts at: 2659 3322 3700 2659
Size: 663 378 4972
Fragments arranged by size:
4972 663 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2259 2259
Size: 6013
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 5160 1678
Size: 3482 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4654 4686 4728 4760 1373
Size: 32 135 32 3082 32 42 32 2626
Fragments arranged by size:
3082 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2395 2427 748
Size: 1647 32 4334
Fragments arranged by size:
4334 1647 32
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2356 5127 5311 1305
Size: 1051 2771 184 2007
Fragments arranged by size:
2771 2007 1051 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4191
Fragments arranged by size:
4191 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2145 2301 3009 3971 4330 4489 518
Size: 648 979 156 708 962 359 159 2042
Fragments arranged by size:
2042 979 962 708 648 359 159 156
MspA1I CmG'CkG
Cuts at: 1606 3205 3429 4106 5254 5499 1606
Size: 1599 224 677 1148 245 2120
Fragments arranged by size:
2120 1599 1148 677 245 224
NcoI C'CATG_G
Cuts at: 513 1065 2173 3165 513
Size: 552 1108 992 3361
Fragments arranged by size:
3361 1108 992 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 2586 2848 3371 4916 1132
Size: 97 1357 262 523 1545 2229
Fragments arranged by size:
2229 1545 1357 523 262 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6013
PciI A'CATG_T
Cuts at: 2582 4912 2582
Size: 2330 3683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6013
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5595 5627 4082
Size: 32 1481 32 4468
Fragments arranged by size:
4468 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 2990 3099 3583 2990
Size: 109 484 5420
Fragments arranged by size:
5420 484 109
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2277 2309 2277
Size: 32 5981
PstI C_TGCA'G
Cuts at: 830 2316 2719 3252 3365 830
Size: 1486 403 533 113 3478
Fragments arranged by size:
3478 1486 533 403 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
PvuII CAG'CTG
Cuts at: 3205 3205
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SapI GCTCTTCn'nnn_
Cuts at: 3021 3021
Size: 6013
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 2799 4261 5973 2799
Size: 1462 1712 2839
Fragments arranged by size:
2839 1712 1462
SfcI C'TryA_G
Cuts at: 826 1045 2312 2715 2801 3248 3270 3361 4496
Size: 219 1267 403 86 447 22 91 1135
Cuts at: 4496 5177 5368 826
Size: 681 191 1471
Fragments arranged by size:
1471 1267 1135 681 447 403 219 191
91 86 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 3340 1872
Size: 1468 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2143 4117 5018 5280 820
Size: 197 926 200 1974 901 262 1553
Fragments arranged by size:
1974 1553 926 901 262 200 197
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 2691 2953 3937 5
Size: 47 2639 262 984 2081
Fragments arranged by size:
2639 2081 984 262 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6013
StyI C'CwwG_G
Cuts at: 513 1065 2173 3165 3452 513
Size: 552 1108 992 287 3074
Fragments arranged by size:
3074 1108 992 552 287
SwaI ATTT'AAAT
Cuts at: 3009 3009
Size: 6013
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2460 2901 4040 4057 4210 4395 4840
Size: 1255 880 441 1139 17 153 185 445
Cuts at: 4840 325
Size: 1498
Fragments arranged by size:
1498 1255 1139 880 445 441 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2128 2797 2956
Size: 274 80 33 51 1443 151 669 159
Cuts at: 2956 4259 5971 96
Size: 1303 1712 138
Fragments arranged by size:
1712 1443 1303 669 274 159 151 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6013
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6013
XmaI C'CCGG_G
Cuts at: 1870 3338 1870
Size: 1468 4545
XmnI GAAnn'nnTTC
Cuts at: 2760 2810 4142 2760
Size: 50 1332 4631
Fragments arranged by size:
4631 1332 50
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI XcmI