Restriction map for
pFN21AB6620
(Circular) (Six-base) MAPSORT of: pFN21AB6620.seq Check: 1515 from: 1 to: 6010
pFN21AB6620 6010 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3353 1937
Size: 1416 4594
Acc65I G'GTAC_C
Cuts at: 3035 3331 3035
Size: 296 5714
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2481 4073 5457 5725 1632
Size: 849 1592 1384 268 1917
Fragments arranged by size:
1917 1592 1384 849 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 2249 4909 5886 1203
Size: 1046 2660 977 1327
Fragments arranged by size:
2660 1327 1046 977
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 3258 1166
Size: 2092 3918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3276 1184
Size: 2092 3918
AlwNI CAG_nnn'CTG
Cuts at: 2612 2884 3753 5325 2612
Size: 272 869 1572 3297
Fragments arranged by size:
3297 1572 869 272
ApaI G_GGCC'C
Cuts at: 2413 3284 2413
Size: 871 5139
ApaLI G'TGCA_C
Cuts at: 4067 5223 4067
Size: 1156 4854
ApoI r'AATT_y
Cuts at: 1493 2260 3319 3545 3697 5806 1493
Size: 767 1059 226 152 2109 1697
Fragments arranged by size:
2109 1697 1059 767 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 2333 2414 3008 3194 3335 1870
Size: 73 390 81 594 186 141 4545
Fragments arranged by size:
4545 594 390 186 141 81 73
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 2198 2956 3035 3234
Size: 325 217 627 314 97 758 79 199
Cuts at: 3234 3260 3331 4786 618
Size: 26 71 1455 1842
Fragments arranged by size:
1842 1455 758 627 325 314 217 199
97 79 71 26
BanII G_rGCy'C
Cuts at: 1391 2076 2163 2413 2472 2928 3201 3284 3329
Size: 685 87 250 59 456 273 83 45
Cuts at: 3329 1391
Size: 4072
Fragments arranged by size:
4072 685 456 273 250 87 83 59
45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2372 2725 928
Size: 971 473 353 4213
Fragments arranged by size:
4213 971 473 353
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2324 2358 4201 4235 1903
Size: 34 50 34 303 34 1843 34 3678
Fragments arranged by size:
3678 1843 303 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2926 3902 5112 2926
Size: 976 1210 3824
Fragments arranged by size:
3824 1210 976
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2196 2454 4621
Size: 107 122 71 631 605 524 258 2167
Cuts at: 4621 136
Size: 1525
Fragments arranged by size:
2167 1525 631 605 524 258 122 107
71
BglII A'GATC_T
Cuts at: 6005 6005
Size: 6010
BlpI GC'TnA_GC
Cuts at: 3427 3427
Size: 6010
Bme1580I G_kGCm'C
Cuts at: 1790 2413 2526 3284 4071 5227 1790
Size: 623 113 758 787 1156 2573
Fragments arranged by size:
2573 1156 787 758 623 113
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2315 2365 2611
Size: 416 53 83 399 24 898 50 246
Cuts at: 2611 2786 2906 2931 2933 3119 3741 4699 442
Size: 175 120 25 2 186 622 958 1753
Fragments arranged by size:
1753 958 898 622 416 399 246 186
175 120 83 53 50 25 24 2
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2589 2919 3150 4670 1239
Size: 1350 330 231 1520 2579
Fragments arranged by size:
2579 1520 1350 330 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2774 4135 5000 5298 2774
Size: 1361 865 298 3486
Fragments arranged by size:
3486 1361 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3126 4673 882
Size: 617 1627 1547 2219
Fragments arranged by size:
2219 1627 1547 617
BsaAI yAC'GTr
Cuts at: 493 1204 3801 493
Size: 711 2597 2702
Fragments arranged by size:
2702 2597 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2797 3817 4199 4834
Size: 53 83 186 936 1264 1020 382 635
Cuts at: 4834 275
Size: 1451
Fragments arranged by size:
1451 1264 1020 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4443 4841 5115 5262 5673 4443
Size: 398 274 147 411 4780
Fragments arranged by size:
4780 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2984 3014 1178
Size: 30 165 30 1581 30 4174
Fragments arranged by size:
4174 1581 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2530 1186
Size: 1165 179 4666
Fragments arranged by size:
4666 1165 179
BseYI C'CCAG_C
Cuts at: 2059 2163 2546 2835 3139 5213 5709 2059
Size: 104 383 289 304 2074 496 2360
Fragments arranged by size:
2360 2074 496 383 304 289 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2335 1163
Size: 573 599 4838
Fragments arranged by size:
4838 599 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3460 4221 5249 1540
Size: 275 186 1459 761 1028 2301
Fragments arranged by size:
2301 1459 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 2472 3255 4071 4156 5227 2277
Size: 195 783 816 85 1071 3060
Fragments arranged by size:
3060 1071 816 783 195 85
BsmI GAATG_Cn'
Cuts at: 3045 3521 3614 3045
Size: 476 93 5441
Fragments arranged by size:
5441 476 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2163 2277 2413 2472 2526 2928
Size: 399 286 87 114 136 59 54 402
Cuts at: 2928 3201 3255 3284 3329 4071 4156 5227 1391
Size: 273 54 29 45 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 286 273 136
114 87 85 59 54 54 45 29
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2138 2639 3365 844
Size: 1294 501 726 3489
Fragments arranged by size:
3489 1294 726 501
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 1394
Size: 429 2074 3507
Fragments arranged by size:
3507 2074 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4505 4679 65
Size: 1183 3257 174 1396
Fragments arranged by size:
3257 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3056 4654 4841 1700
Size: 110 1246 1598 187 2869
Fragments arranged by size:
2869 1598 1246 187 110
BsrGI T'GTAC_A
Cuts at: 96 2420 96
Size: 2324 3686
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2178 794
Size: 1384 4626
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 3221 3221
Size: 6010
BstYI r'GATC_y
Cuts at: 1972 3340 3707 4092 4109 5561 5647 5659 6005
Size: 1368 367 385 17 1452 86 12 346
Cuts at: 6005 1972
Size: 1977
Fragments arranged by size:
1977 1452 1368 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3031 513
Size: 552 1966 3492
Fragments arranged by size:
3492 1966 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2070 487
Size: 683 56 844 4427
Fragments arranged by size:
4427 844 683 56
BtsI GCAGTG_nn'
Cuts at: 2425 2500 2610 2651 2882 3526 4320 4340 2425
Size: 75 110 41 231 644 794 20 4095
Fragments arranged by size:
4095 794 644 231 110 75 41 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DraIII CAC_nnn'GTG
Cuts at: 2574 2574
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3123 5017 809
Size: 507 1807 1894 1802
Fragments arranged by size:
1894 1807 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2688 3457 4346
Size: 54 1425 50 275 18 858 769 889
Cuts at: 4346 5975 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 858 769 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3457 1537
Size: 275 1645 4090
Fragments arranged by size:
4090 1645 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2744 3168 3940 1353
Size: 719 672 424 772 3423
Fragments arranged by size:
3423 772 719 672 424
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4597 4971 5117 387
Size: 1064 138 3008 374 146 1280
Fragments arranged by size:
3008 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2470 2470
Size: 6010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2481 2589 2919 3150 4073 4670 5457
Size: 393 849 108 330 231 923 597 787
Cuts at: 5457 5725 1239
Size: 268 1524
Fragments arranged by size:
1524 923 849 787 597 393 330 268
231 108
EcoNI CCTnn'n_nnAGG
Cuts at: 3274 3274
Size: 6010
EcoO109I rG'GnC_Cy
Cuts at: 2048 2410 2813 3125 3181 3280 2048
Size: 362 403 312 56 99 4778
Fragments arranged by size:
4778 403 362 312 99 56
EcoRI G'AATT_C
Cuts at: 2260 3319 3697 2260
Size: 1059 378 4573
Fragments arranged by size:
4573 1059 378
FspI TGC'GCA
Cuts at: 4516 4516
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 2553 5157 1678
Size: 875 2604 2531
Fragments arranged by size:
2604 2531 875
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2772 2804 4651 4683 4725
Size: 32 135 32 1200 32 1847 32 42
Cuts at: 4725 4757 1373
Size: 32 2626
Fragments arranged by size:
2626 1847 1200 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 3600 669
Size: 988 281 1416 246 3079
Fragments arranged by size:
3079 1416 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KpnI G_GTAC'C
Cuts at: 3039 3335 3039
Size: 296 5714
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 2646 2982 5124 5308 1305
Size: 792 549 336 2142 184 2007
Fragments arranged by size:
2142 2007 792 549 336 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4188
Fragments arranged by size:
4188 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2681 3258 3968 4327 4486 518
Size: 648 1515 577 710 359 159 2042
Fragments arranged by size:
2042 1515 710 648 577 359 159
MspA1I CmG'CkG
Cuts at: 1606 2187 2302 2379 2556 3426 4103 5251 5496
Size: 581 115 77 177 870 677 1148 245
Cuts at: 5496 1606
Size: 2120
Fragments arranged by size:
2120 1148 870 677 581 245 177 115
77
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5458
NdeI CA'TA_TG
Cuts at: 387 2110 387
Size: 1723 4287
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 2253 3368 4913 1132
Size: 97 1024 1115 1545 2229
Fragments arranged by size:
2229 1545 1115 1024 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6010
PciI A'CATG_T
Cuts at: 2249 4909 2249
Size: 2660 3350
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2590 1108
Size: 1482 4528
PfoI T'CCnGG_A
Cuts at: 2229 3127 2229
Size: 898 5112
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2813 3125 3181 2813
Size: 312 56 5642
Fragments arranged by size:
5642 312 56
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PspOMI G'GGCC_C
Cuts at: 2409 3280 2409
Size: 871 5139
PspXI vC'TCGA_Gb
Cuts at: 2333 2414 2333
Size: 81 5929
PstI C_TGCA'G
Cuts at: 830 2135 2771 3362 830
Size: 1305 636 591 3478
Fragments arranged by size:
3478 1305 636 591
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2302 2302
Size: 6010
SacI G_AGCT'C
Cuts at: 2472 2472
Size: 6010
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SanDI GG'GwC_CC
Cuts at: 2813 3181 2813
Size: 368 5642
SapI GCTCTTCn'nnn_
Cuts at: 2744 2744
Size: 6010
SbfI CC_TGCA'GG
Cuts at: 2135 3362 2135
Size: 1227 4783
ScaI AGT'ACT
Cuts at: 3108 4258 5970 3108
Size: 1150 1712 3148
Fragments arranged by size:
3148 1712 1150
SexAI A'CCwGG_T
Cuts at: 2570 2570
Size: 6010
SfcI C'TryA_G
Cuts at: 826 1045 2131 2767 2823 3358 4493 5174 5365
Size: 219 1086 636 56 535 1135 681 191
Cuts at: 5365 826
Size: 1471
Fragments arranged by size:
1471 1135 1086 681 636 535 219 191
56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 3056 1700
Size: 1356 4654
SmaI CCC'GGG
Cuts at: 1872 3010 3196 3337 1872
Size: 1138 186 141 4545
Fragments arranged by size:
4545 1138 186 141
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2333 2414 2789 4114 5015 5277
Size: 197 926 390 81 375 1325 901 262
Cuts at: 5277 820
Size: 1553
Fragments arranged by size:
1553 1325 926 901 390 375 262 197
81
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 3934 5
Size: 47 3882 2081
Fragments arranged by size:
3882 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6010
StyI C'CwwG_G
Cuts at: 513 1065 2015 2584 3284 3449 513
Size: 552 950 569 700 165 3074
Fragments arranged by size:
3074 950 700 569 552 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3317 4037 4054 4207 4392 4837 325
Size: 1255 1737 720 17 153 185 445 1498
Fragments arranged by size:
1737 1498 1255 720 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2420 3106 4256
Size: 274 80 33 51 1443 443 686 1150
Cuts at: 4256 5968 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1150 686 443 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5970 6002 5970
Size: 32 5978
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2683 3038 3218 2683
Size: 355 180 5475
Fragments arranged by size:
5475 355 180
XhoI C'TCGA_G
Cuts at: 1943 2333 2414 1943
Size: 390 81 5539
Fragments arranged by size:
5539 390 81
XmaI C'CCGG_G
Cuts at: 1870 3008 3194 3335 1870
Size: 1138 186 141 4545
Fragments arranged by size:
4545 1138 186 141
XmnI GAAnn'nnTTC
Cuts at: 4139 4139
Size: 6010
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PsrI RsrII SacII SfiI SfoI
SrfI SwaI