Restriction map for
pFN21AB6617
(Circular) (Six-base) MAPSORT of: pFN21AB6617.seq Check: 6905 from: 1 to: 5941
pFN21AB6617 5941 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2074 2345 2848 2074
Size: 271 503 5167
Fragments arranged by size:
5167 503 271
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 2345 3262 2345
Size: 917 5024
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 2630 4004 5388 5656 1632
Size: 581 417 1374 1384 268 1917
Fragments arranged by size:
1917 1384 1374 581 417 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3041 1184
Size: 1857 4084
AlwNI CAG_nnn'CTG
Cuts at: 2869 2947 3684 5256 2869
Size: 78 737 1572 3554
Fragments arranged by size:
3554 1572 737 78
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2223 2486 3266 1870
Size: 73 280 263 780 4545
Fragments arranged by size:
4545 780 280 263 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2345 2357 3171 3262 4717
Size: 325 217 627 558 12 814 91 1455
Cuts at: 4717 618
Size: 1842
Fragments arranged by size:
1842 1455 814 627 558 325 217 91
12
BanII G_rGCy'C
Cuts at: 1391 2046 2114 2941 3087 3260 1391
Size: 655 68 827 146 173 4072
Fragments arranged by size:
4072 827 655 173 146 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2410 928
Size: 971 511 4459
Fragments arranged by size:
4459 971 511
BbvCI CC'TCA_GC
Cuts at: 2411 2411
Size: 5941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2661 2670 2903
Size: 107 122 71 631 605 989 9 233
Cuts at: 2903 3102 4552 136
Size: 199 1450 1525
Fragments arranged by size:
1525 1450 989 631 605 233 199 122
107 71 9
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 2972 3358 2972
Size: 386 5555
Bme1580I G_kGCm'C
Cuts at: 1790 2362 4002 5158 1790
Size: 572 1640 1156 2573
Fragments arranged by size:
2573 1640 1156 572
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2123 2252 2273 2453 2623 2810 4601
Size: 791 93 129 21 180 170 187 1791
Cuts at: 4601 1239
Size: 2579
Fragments arranged by size:
2579 1791 791 187 180 170 129 93
21
Bpu10I CC'TnA_GC
Cuts at: 2384 2411 2384
Size: 27 5914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2966 4066 4931 5229 2966
Size: 1100 865 298 3678
Fragments arranged by size:
3678 1100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2478 3748 4130 4765
Size: 53 83 186 936 945 1270 382 635
Cuts at: 4765 275
Size: 1451
Fragments arranged by size:
1451 1270 945 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2772 4374 4772 5046 5193 5604 2772
Size: 1602 398 274 147 411 3109
Fragments arranged by size:
3109 1602 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2361 2391 1178
Size: 30 165 30 958 30 4728
Fragments arranged by size:
4728 958 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2708 2969 3089 1186
Size: 1522 261 120 4038
Fragments arranged by size:
4038 1522 261 120
BseYI C'CCAG_C
Cuts at: 2140 2361 2659 2872 2889 5144 5640 2140
Size: 221 298 213 17 2255 496 2441
Fragments arranged by size:
2441 2255 496 298 221 213 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2882 2972 1163
Size: 573 1146 90 4132
Fragments arranged by size:
4132 1146 573 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2114 3087 4002 4087 5158 2114
Size: 973 915 85 1071 2897
Fragments arranged by size:
2897 1071 973 915 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
BsmBI CGTCTCn'nnnn_
Cuts at: 3071 3071
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2114 2362 2941 3087 3260 4002
Size: 399 256 68 248 579 146 173 742
Cuts at: 4002 4087 5158 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 399 256 248 173
146 85 68
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 2829 3830 5560 2829
Size: 1001 1730 3210
Fragments arranged by size:
3210 1730 1001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2074 2336 2345 2848 3296 844
Size: 1230 262 9 503 448 3489
Fragments arranged by size:
3489 1230 503 448 262 9
BsrBI CCG'CTC
Cuts at: 1394 1823 3216 3828 1394
Size: 429 1393 612 3507
Fragments arranged by size:
3507 1393 612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2078 2401 4436 4610 65
Size: 1183 830 323 2035 174 1396
Fragments arranged by size:
2035 1396 1183 830 323 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2727 3129 4585 4772 1700
Size: 110 917 402 1456 187 2869
Fragments arranged by size:
2869 1456 917 402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 3062 4001 5013 3062
Size: 939 1012 3990
Fragments arranged by size:
3990 1012 939
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2328 2869 794
Size: 1534 541 3866
Fragments arranged by size:
3866 1534 541
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 2944 3271 3638 4023 4040 5492 5578 5590
Size: 972 327 367 385 17 1452 86 12
Cuts at: 5590 5936 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 972 385 367 346 327 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3167 3167
Size: 5941
BtgI C'CryG_G
Cuts at: 513 1065 2004 2643 2679 2779 513
Size: 552 939 639 36 100 3675
Fragments arranged by size:
3675 939 639 552 100 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2680 2732 3457 4251 4271 2680
Size: 52 725 794 20 4350
Fragments arranged by size:
4350 794 725 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DraIII CAC_nnn'GTG
Cuts at: 3052 3052
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2640 2729 2880
Size: 54 1425 50 275 18 810 89 151
Cuts at: 2880 2895 2949 3388 4277 5906 8
Size: 15 54 439 889 1629 43
Fragments arranged by size:
1629 1425 889 810 439 275 151 89
54 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 3871 1353
Size: 749 1769 3423
Fragments arranged by size:
3423 1769 749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2806 4528 4902 5048 387
Size: 1064 138 1217 1722 374 146 1280
Fragments arranged by size:
1722 1280 1217 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2112 3085 2112
Size: 973 4968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2123 2213 2252 2273 2453 2623
Size: 393 398 93 90 39 21 180 170
Cuts at: 2623 2630 2810 4004 4601 5388 5656 1239
Size: 7 180 1194 597 787 268 1524
Fragments arranged by size:
1524 1194 787 597 398 393 268 180
180 170 93 90 39 21 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2803 2855 2803
Size: 52 5889
EcoO109I rG'GnC_Cy
Cuts at: 2428 2449 2785 3139 3189 2428
Size: 21 336 354 50 5180
Fragments arranged by size:
5180 354 336 50 21
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3250 3250
Size: 5941
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2131 2744 2870 2909 5088 1678
Size: 453 613 126 39 2179 2531
Fragments arranged by size:
2531 2179 613 453 126 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2361 2393 4582 4614 4656
Size: 32 135 32 789 32 2189 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 2189 789 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2584 3285 3531 669
Size: 988 281 646 701 246 3079
Fragments arranged by size:
3079 988 701 646 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 2349 3266 2349
Size: 917 5024
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 5055 5239 1305
Size: 780 2970 184 2007
Fragments arranged by size:
2970 2007 780 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2882 2897 10
Size: 54 1425 343 1050 15 3054
Fragments arranged by size:
3054 1425 1050 343 54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2469 2642 3899 4258 4417 518
Size: 648 1303 173 1257 359 159 2042
Fragments arranged by size:
2042 1303 1257 648 359 173 159
MspA1I CmG'CkG
Cuts at: 1606 2050 3031 3357 4034 5182 5427 1606
Size: 444 981 326 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 677 444 326 245
NaeI GCC'GGC
Cuts at: 3131 3131
Size: 5941
NcoI C'CATG_G
Cuts at: 513 1065 2004 2679 513
Size: 552 939 675 3775
Fragments arranged by size:
3775 939 675 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 3129 3129
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2889 2947 1108
Size: 1781 58 4102
Fragments arranged by size:
4102 1781 58
PfoI T'CCnGG_A
Cuts at: 2250 2250
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2785 3189 2785
Size: 404 5537
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspXI vC'TCGA_Gb
Cuts at: 2486 2486
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2507 2718 2739 3030 3138 3180 3293 830
Size: 1677 211 21 291 108 42 113 3478
Fragments arranged by size:
3478 1677 291 211 113 108 42 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2050 2050
Size: 5941
SacI G_AGCT'C
Cuts at: 2114 3087 2114
Size: 973 4968
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 3189 3189
Size: 5941
SapI GCTCTTCn'nnn_
Cuts at: 2102 2102
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 2503 2714 2735 3026 3134 3176 3289
Size: 219 1458 211 21 291 108 42 113
Cuts at: 3289 4424 5105 5296 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1458 1135 681 291 219 211 191
113 108 42 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2225 3268 1872
Size: 353 1043 4545
Fragments arranged by size:
4545 1043 353
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 2981 4045 4946 5208 820
Size: 197 926 543 495 1064 901 262 1553
Fragments arranged by size:
1553 1064 926 901 543 495 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 2332 2679 3380 513
Size: 552 939 328 347 701 3074
Fragments arranged by size:
3074 939 701 552 347 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2849 2864 2881 3968 3985 4138 4323
Size: 1255 1269 15 17 1087 17 153 185
Cuts at: 4323 4768 325
Size: 445 1498
Fragments arranged by size:
1498 1269 1255 1087 445 185 153 17
17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4187 5899 96
Size: 274 80 33 51 1443 2210 1712 138
Fragments arranged by size:
2210 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2777 2890 2905 2777
Size: 113 15 5813
Fragments arranged by size:
5813 113 15
XhoI C'TCGA_G
Cuts at: 1943 2486 1943
Size: 543 5398
XmaI C'CCGG_G
Cuts at: 1870 2223 3266 1870
Size: 353 1043 4545
Fragments arranged by size:
4545 1043 353
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI