Restriction map for
pFN21AB6616
(Circular) (Six-base) MAPSORT of: pFN21AB6616.seq Check: 7230 from: 1 to: 6004
pFN21AB6616 6004 bp
With 182 enzymes: SgfI *
June 30, 2009 16:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2639 2639
Size: 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2973 4067 5451 5719 1632
Size: 1341 1094 1384 268 1917
Fragments arranged by size:
1917 1384 1341 1094 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 2073 4903 5880 1203
Size: 870 2830 977 1327
Fragments arranged by size:
2830 1327 977 870
AgeI A'CCGG_T
Cuts at: 2561 2994 4835 2561
Size: 433 1841 3730
Fragments arranged by size:
3730 1841 433
AhdI GACnn_n'nnGTC
Cuts at: 2719 4733 2719
Size: 2014 3990
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 2029 3747 5319 2029
Size: 1718 1572 2714
Fragments arranged by size:
2714 1718 1572
ApaI G_GGCC'C
Cuts at: 3235 3235
Size: 6004
ApaLI G'TGCA_C
Cuts at: 2522 4061 5217 2522
Size: 1539 1156 3309
Fragments arranged by size:
3309 1539 1156
ApoI r'AATT_y
Cuts at: 1493 2298 3313 3539 3691 5800 1493
Size: 805 1015 226 152 2109 1697
Fragments arranged by size:
2109 1697 1015 805 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 2669 2827 3329 1870
Size: 73 726 158 502 4545
Fragments arranged by size:
4545 726 502 158 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2461 2494 2461
Size: 33 5971
BamHI G'GATC_C
Cuts at: 2265 3334 3701 2265
Size: 1069 367 4568
Fragments arranged by size:
4568 1069 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2567 3016 3106 3325 4780
Size: 325 217 627 780 449 90 219 1455
Cuts at: 4780 618
Size: 1842
Fragments arranged by size:
1842 1455 780 627 449 325 219 217
90
BanII G_rGCy'C
Cuts at: 1391 2651 2981 3235 3323 1391
Size: 1260 330 254 88 4072
Fragments arranged by size:
4072 1260 330 254 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5033
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3251 4615 136
Size: 107 122 71 631 605 1579 1364 1525
Fragments arranged by size:
1579 1525 1364 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 3203 3421 3203
Size: 218 5786
Bme1580I G_kGCm'C
Cuts at: 1790 2526 2967 3111 3235 4065 5221 1790
Size: 736 441 144 124 830 1156 2573
Fragments arranged by size:
2573 1156 830 736 441 144 124
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2444 3735 4693
Size: 416 53 83 399 24 1027 1291 958
Cuts at: 4693 442
Size: 1753
Fragments arranged by size:
1753 1291 1027 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2516 4664 1239
Size: 1277 2148 2579
Fragments arranged by size:
2579 2148 1277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2894 4129 4994 5292 2228
Size: 666 1235 865 298 2940
Fragments arranged by size:
2940 1235 865 666 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4667 882
Size: 617 3168 2219
Fragments arranged by size:
3168 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2561 2931 2994 4437 4835 5109 5256 5667 2561
Size: 370 63 1443 398 274 147 411 2898
Fragments arranged by size:
2898 1443 411 398 370 274 147 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 2681 2711 1178
Size: 30 165 30 801 30 447 30 4471
Fragments arranged by size:
4471 801 447 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2326 3143 1186
Size: 1140 817 4047
Fragments arranged by size:
4047 1140 817
BseYI C'CCAG_C
Cuts at: 2029 2167 2726 5207 5703 2029
Size: 138 559 2481 496 2330
Fragments arranged by size:
2481 2330 559 496 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2242 2591 3020 1163
Size: 573 506 349 429 4147
Fragments arranged by size:
4147 573 506 429 349
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2526 3209 4065 4150 5221 2526
Size: 683 856 85 1071 3309
Fragments arranged by size:
3309 1071 856 683 85
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
BsmBI CGTCTCn'nnnn_
Cuts at: 2207 2207
Size: 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2526 2651 2967 2981 3111 3209 3235
Size: 399 736 125 316 14 130 98 26
Cuts at: 3235 3323 4065 4150 5221 1391
Size: 88 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 736 399 316 130 125
98 88 85 26 14
BspEI T'CCGG_A
Cuts at: 2931 5667 2931
Size: 2736 3268
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 2511 2556 2639 2865 3042 3292 3359
Size: 1328 339 45 83 226 177 250 67
Cuts at: 3359 844
Size: 3489
Fragments arranged by size:
3489 1328 339 250 226 177 83 67
45
BsrBI CCG'CTC
Cuts at: 1394 1823 2848 3891 1394
Size: 429 1025 1043 3507
Fragments arranged by size:
3507 1043 1025 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4499 4673 65
Size: 1183 3251 174 1396
Fragments arranged by size:
3251 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2561 2884 2994 3279 4648 4835 1700
Size: 110 751 323 110 285 1369 187 2869
Fragments arranged by size:
2869 1369 751 323 285 187 110 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2419 3255 794
Size: 1625 836 3543
Fragments arranged by size:
3543 1625 836
BstBI TT'CG_AA
Cuts at: 2769 4808 2769
Size: 2039 3965
BstEII G'GTnAC_C
Cuts at: 1184 2731 1184
Size: 1547 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2686 2011
Size: 675 5329
BstYI r'GATC_y
Cuts at: 1972 2238 2265 2910 3273 3334 3701 4086 4103
Size: 266 27 645 363 61 367 385 17
Cuts at: 4103 5555 5641 5653 5999 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 645 385 367 363 346 266
86 61 27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2464 2751 513
Size: 552 939 460 287 3766
Fragments arranged by size:
3766 939 552 460 287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 2603 3520 4314 4334 2603
Size: 917 794 20 4273
Fragments arranged by size:
4273 917 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2595 2886 3451
Size: 54 1425 50 275 18 765 291 565
Cuts at: 3451 4340 5969 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 765 565 291 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2358 3068 3120 3934 1353
Size: 1005 710 52 814 3423
Fragments arranged by size:
3423 1005 814 710 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2636 3236 3250 3266 4591 4965
Size: 1064 138 1047 600 14 16 1325 374
Cuts at: 4965 5111 387
Size: 146 1280
Fragments arranged by size:
1325 1280 1064 1047 600 374 146 138
16 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2516 2973 4067 4664 5451 5719 1239
Size: 393 884 457 1094 597 787 268 1524
Fragments arranged by size:
1524 1094 884 787 597 457 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3164 3164
Size: 6004
EcoO109I rG'GnC_Cy
Cuts at: 2161 3031 3058 3118 3157 3232 2161
Size: 870 27 60 39 75 4933
Fragments arranged by size:
4933 870 75 60 39 27
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2888 2888
Size: 6004
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 3154 5151 1678
Size: 1476 1997 2531
Fragments arranged by size:
2531 1997 1476
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2681 2705 2713
Size: 32 135 32 470 32 607 24 8
Cuts at: 2713 2737 4645 4677 4719 4751 1373
Size: 24 1908 32 42 32 2626
Fragments arranged by size:
2626 1908 607 470 135 42 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2607 5118 5302 1305
Size: 1302 2511 184 2007
Fragments arranged by size:
2511 2007 1302 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4182
Fragments arranged by size:
4182 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2328 3011 3962 4321 4480 518
Size: 648 843 319 683 951 359 159 2042
Fragments arranged by size:
2042 951 843 683 648 359 319 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2341 2482 2925 3202 3420 4097 5245
Size: 423 312 141 443 277 218 677 1148
Cuts at: 5245 5490 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 443 423 312 277 245
218 141
NaeI GCC'GGC
Cuts at: 3281 3281
Size: 6004
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4513
Fragments arranged by size:
4513 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NgoMIV G'CCGG_C
Cuts at: 3279 3279
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2038 2413 3362 4907 1132
Size: 97 809 375 949 1545 2229
Fragments arranged by size:
2229 1545 949 809 375 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6004
PciI A'CATG_T
Cuts at: 4903 4903
Size: 6004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 1108
Size: 1236 4768
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2161 3031 3058 3118 2161
Size: 870 27 60 5047
Fragments arranged by size:
5047 870 60 27
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PspOMI G'GGCC_C
Cuts at: 3231 3231
Size: 6004
PstI C_TGCA'G
Cuts at: 830 3356 830
Size: 2526 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 2029 2341 2482 2925 3202 2029
Size: 312 141 443 277 4831
Fragments arranged by size:
4831 443 312 277 141
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 2396 4252 5964 2396
Size: 1856 1712 2436
Fragments arranged by size:
2436 1856 1712
SfcI C'TryA_G
Cuts at: 826 1045 3352 4487 5168 5359 826
Size: 219 2307 1135 681 191 1471
Fragments arranged by size:
2307 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 2561 1700
Size: 861 5143
SmaI CCC'GGG
Cuts at: 1872 2829 3331 1872
Size: 957 502 4545
Fragments arranged by size:
4545 957 502
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 2873 4108 5009 5271 820
Size: 197 926 300 630 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 630 300 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 2413 3362 1132
Size: 1281 949 3774
Fragments arranged by size:
3774 1281 949
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 3254 1517
Size: 1737 4267
StyI C'CwwG_G
Cuts at: 513 1065 2004 2988 3165 3443 513
Size: 552 939 984 177 278 3074
Fragments arranged by size:
3074 984 939 552 278 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2394 4250 5962
Size: 274 80 33 51 1443 417 1856 1712
Cuts at: 5962 96
Size: 138
Fragments arranged by size:
1856 1712 1443 417 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 5964 5996 2204
Size: 32 3728 32 2212
Fragments arranged by size:
3728 2212 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 2827 3329 1870
Size: 957 502 4545
Fragments arranged by size:
4545 957 502
XmnI GAAnn'nnTTC
Cuts at: 2444 4133 2444
Size: 1689 4315
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI