Restriction map for
pFN21AB6614
(Circular) (Six-base) MAPSORT of: pFN21AB6614.seq Check: 3567 from: 1 to: 6001
pFN21AB6614 6001 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3344 1937
Size: 1407 4594
Acc65I G'GTAC_C
Cuts at: 2361 3322 2361
Size: 961 5040
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 2815 4064 5448 5716 1632
Size: 1046 137 1249 1384 268 1917
Fragments arranged by size:
1917 1384 1249 1046 268 137
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 4900 5877 1203
Size: 3697 977 1327
Fragments arranged by size:
3697 1327 977
AgeI A'CCGG_T
Cuts at: 3208 4832 3208
Size: 1624 4377
AhdI GACnn_n'nnGTC
Cuts at: 3219 4730 3219
Size: 1511 4490
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3162 1184
Size: 1978 4023
AlwNI CAG_nnn'CTG
Cuts at: 2539 3037 3744 5316 2539
Size: 498 707 1572 3224
Fragments arranged by size:
3224 1572 707 498
ApaI G_GGCC'C
Cuts at: 2385 2385
Size: 6001
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 3310 3536 3688 5797 1493
Size: 1817 226 152 2109 1697
Fragments arranged by size:
2109 1817 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 2159 2377 3326 1870
Size: 73 216 218 949 4545
Fragments arranged by size:
4545 949 218 216 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2361 3322 4777 618
Size: 325 217 627 324 250 961 1455 1842
Fragments arranged by size:
1842 1455 961 627 325 324 250 217
BanII G_rGCy'C
Cuts at: 1391 2054 2385 2913 3320 1391
Size: 663 331 528 407 4072
Fragments arranged by size:
4072 663 528 407 331
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3131 928
Size: 971 1232 3798
Fragments arranged by size:
3798 1232 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2140 2174 4192 4226 1903
Size: 34 203 34 2018 34 3678
Fragments arranged by size:
3678 2018 203 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 5103 3893
Size: 1210 4791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2067 2495 4612
Size: 107 122 71 631 605 395 428 2117
Cuts at: 4612 136
Size: 1525
Fragments arranged by size:
2117 1525 631 605 428 395 122 107
71
BglII A'GATC_T
Cuts at: 2901 5996 2901
Size: 3095 2906
BlpI GC'TnA_GC
Cuts at: 2957 3418 2957
Size: 461 5540
Bme1580I G_kGCm'C
Cuts at: 1790 2317 2385 4062 5218 1790
Size: 527 68 1677 1156 2573
Fragments arranged by size:
2573 1677 1156 527 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3732 4690 442
Size: 416 53 83 399 24 2315 958 1753
Fragments arranged by size:
2315 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2161 2268 2339 2360 2636 2996 3155
Size: 781 141 107 71 21 276 360 159
Cuts at: 3155 3188 4661 1239
Size: 33 1473 2579
Fragments arranged by size:
2579 1473 781 360 276 159 141 107
71 33 21
Bpu10I CC'TnA_GC
Cuts at: 2750 2750
Size: 6001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2621 4126 4991 5289 2621
Size: 1505 865 298 3333
Fragments arranged by size:
3333 1505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4664 882
Size: 617 3165 2219
Fragments arranged by size:
3165 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3792 493
Size: 711 2588 2702
Fragments arranged by size:
2702 2588 711
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3808 4190 4825 275
Size: 53 83 186 936 2275 382 635 1451
Fragments arranged by size:
2275 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3208 4434 4832 5106 5253 5664 3208
Size: 1226 398 274 147 411 3545
Fragments arranged by size:
3545 1226 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5776
Fragments arranged by size:
5776 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2044 2336 2435 2657 2687 2690 3068
Size: 837 21 292 99 222 30 3 378
Cuts at: 3068 3259 1186
Size: 191 3928
Fragments arranged by size:
3928 837 378 292 222 191 99 30
21 3
BseYI C'CCAG_C
Cuts at: 2128 2952 3066 5204 5700 2128
Size: 824 114 2138 496 2429
Fragments arranged by size:
2429 2138 824 496 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2903 3035 1163
Size: 573 1167 132 4129
Fragments arranged by size:
4129 1167 573 132
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4212 5240 1540
Size: 275 186 1450 761 1028 2301
Fragments arranged by size:
2301 1450 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2913 4062 4147 5218 2913
Size: 1149 85 1071 3696
Fragments arranged by size:
3696 1149 1071 85
BsmI GAATG_Cn'
Cuts at: 3512 3605 3512
Size: 93 5908
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2317 2385 2913 3320 4062 4147
Size: 399 264 263 68 528 407 742 85
Cuts at: 4147 5218 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 528 407 399 264 263
85 68
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3271 3356 844
Size: 2427 85 3489
Fragments arranged by size:
3489 2427 85
BsrBI CCG'CTC
Cuts at: 1394 1823 3888 1394
Size: 429 2065 3507
Fragments arranged by size:
3507 2065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4496 4670 65
Size: 1183 3248 174 1396
Fragments arranged by size:
3248 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 2114 3208 4645 4832 1700
Size: 110 281 23 1094 1437 187 2869
Fragments arranged by size:
2869 1437 1094 281 187 110 23
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 2669 4061 5073 2669
Size: 1392 1012 3597
Fragments arranged by size:
3597 1392 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2088 2539 794
Size: 1294 451 4256
Fragments arranged by size:
4256 1294 451
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 3211 1184
Size: 2027 3974
BstXI CCAn_nnnn'nTGG
Cuts at: 2705 2705
Size: 6001
BstYI r'GATC_y
Cuts at: 1972 2132 2349 2436 2901 3027 3331 3698 4083
Size: 160 217 87 465 126 304 367 385
Cuts at: 4083 4100 5552 5638 5650 5996 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 465 385 367 346 304 217
160 126 87 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2451 513
Size: 552 1386 4063
Fragments arranged by size:
4063 1386 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5262
Fragments arranged by size:
5262 683 56
BtsI GCAGTG_nn'
Cuts at: 3517 4311 4331 3517
Size: 794 20 5187
Fragments arranged by size:
5187 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2601 2862 3448
Size: 54 1425 50 275 18 771 261 586
Cuts at: 3448 4337 5966 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 771 586 275 261 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3448 1537
Size: 275 1636 4090
Fragments arranged by size:
4090 1636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3237 3931 1353
Size: 1884 694 3423
Fragments arranged by size:
3423 1884 694
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2009 3005 4588 4962 5108 387
Size: 1064 138 420 996 1583 374 146 1280
Fragments arranged by size:
1583 1280 1064 996 420 374 146 138
EcoICRI GAG'CTC
Cuts at: 2911 2911
Size: 6001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2161 2268 2339 2360 2636 2678
Size: 393 388 141 107 71 21 276 42
Cuts at: 2678 2815 2996 3155 3188 4064 4661 5448 5716
Size: 137 181 159 33 876 597 787 268
Cuts at: 5716 1239
Size: 1524
Fragments arranged by size:
1524 876 787 597 393 388 276 268
181 159 141 137 107 71 42 33
21
EcoNI CCTnn'n_nnAGG
Cuts at: 2303 2321 2639 3167 2303
Size: 18 318 528 5137
Fragments arranged by size:
5137 528 318 18
EcoO109I rG'GnC_Cy
Cuts at: 2374 2381 2536 2374
Size: 7 155 5839
Fragments arranged by size:
5839 155 7
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FspI TGC'GCA
Cuts at: 2576 4507 2576
Size: 1931 4070
HaeII r_GCGC'y
Cuts at: 1678 5148 1678
Size: 3470 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2933 2965 3205 3237 4642
Size: 32 135 32 1361 32 240 32 1405
Cuts at: 4642 4674 4716 4748 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1405 1361 240 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KpnI G_GTAC'C
Cuts at: 2365 3326 2365
Size: 961 5040
MfeI C'AATT_G
Cuts at: 2415 3600 2415
Size: 1185 4816
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 5299 1305
Size: 3810 184 2007
Fragments arranged by size:
3810 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2603 2864 10
Size: 54 1425 343 771 261 3147
Fragments arranged by size:
3147 1425 771 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3959 4318 4477 518
Size: 648 2793 359 159 2042
Fragments arranged by size:
2793 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2181 3019 3061 3417 4094 5242 5487 1606
Size: 575 838 42 356 677 1148 245 2120
Fragments arranged by size:
2120 1148 838 677 575 356 245 42
NaeI GCC'GGC
Cuts at: 2093 2116 2093
Size: 23 5978
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5449
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NgoMIV G'CCGG_C
Cuts at: 2091 2114 2091
Size: 23 5978
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 3359 4904 1132
Size: 97 2130 1545 2229
Fragments arranged by size:
2229 2130 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6001
PfoI T'CCnGG_A
Cuts at: 3249 3249
Size: 6001
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 4070 4102 5583 5615 2810
Size: 32 1228 32 1481 32 3196
Fragments arranged by size:
3196 1481 1228 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PspOMI G'GGCC_C
Cuts at: 2381 2381
Size: 6001
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 5969
PstI C_TGCA'G
Cuts at: 830 2126 2430 2949 3353 830
Size: 1296 304 519 404 3478
Fragments arranged by size:
3478 1296 519 404 304
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 2181 3019 3061 2181
Size: 838 42 5121
Fragments arranged by size:
5121 838 42
SacI G_AGCT'C
Cuts at: 2913 2913
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SbfI CC_TGCA'GG
Cuts at: 2126 3353 2126
Size: 1227 4774
ScaI AGT'ACT
Cuts at: 2405 4249 5961 2405
Size: 1844 1712 2445
Fragments arranged by size:
2445 1844 1712
SfcI C'TryA_G
Cuts at: 826 1045 2122 2185 2395 2407 2426 2474 2945
Size: 219 1077 63 210 12 19 48 471
Cuts at: 2945 3349 4484 5165 5356 826
Size: 404 1135 681 191 1471
Fragments arranged by size:
1471 1135 1077 681 471 404 219 210
191 63 48 19 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 2379 3328 1872
Size: 507 949 4545
Fragments arranged by size:
4545 949 507
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2636 4105 5006 5268 820
Size: 197 926 693 1469 901 262 1553
Fragments arranged by size:
1553 1469 926 901 693 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 2930 1517
Size: 1413 4588
StyI C'CwwG_G
Cuts at: 513 1065 2931 3440 513
Size: 552 1866 509 3074
Fragments arranged by size:
3074 1866 552 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2099 2693 4028 4045 4198 4383 4828
Size: 1255 519 594 1335 17 153 185 445
Cuts at: 4828 325
Size: 1498
Fragments arranged by size:
1498 1335 1255 594 519 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2403 3195 4247
Size: 274 80 33 51 1443 426 792 1052
Cuts at: 4247 5959 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1052 792 426 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5961 5993 5961
Size: 32 5969
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 2611 2875 2929 3038 2335
Size: 276 264 54 109 5298
Fragments arranged by size:
5298 276 264 109 54
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 2377 3326 1870
Size: 507 949 4545
Fragments arranged by size:
4545 949 507
XmnI GAAnn'nnTTC
Cuts at: 4130 4130
Size: 6001
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspXI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI