Restriction map for
pFN21AB6611
(Circular) (Six-base) MAPSORT of: pFN21AB6611.seq Check: 8519 from: 1 to: 5941
pFN21AB6611 5941 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2986 2986
Size: 5941
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4004 5388 5656 1632
Size: 2372 1384 268 1917
Fragments arranged by size:
2372 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 2922 4840 5817 1203
Size: 1719 1918 977 1327
Fragments arranged by size:
1918 1719 1327 977
AgeI A'CCGG_T
Cuts at: 2310 4772 2310
Size: 2462 3479
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 2979 1166
Size: 1813 4128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2628 1184
Size: 1444 4497
AlwNI CAG_nnn'CTG
Cuts at: 2068 3684 5256 2068
Size: 1616 1572 2753
Fragments arranged by size:
2753 1616 1572
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2014 3250 3476 3628 5737 1493
Size: 521 1236 226 152 2109 1697
Fragments arranged by size:
2109 1697 1236 521 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2590 3266 1870
Size: 73 647 676 4545
Fragments arranged by size:
4545 676 647 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2304 2328 2569 2885 3262
Size: 325 217 627 517 24 241 316 377
Cuts at: 3262 4717 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 517 377 325 316 241
217 24
BanII G_rGCy'C
Cuts at: 1391 2409 3087 3168 3260 1391
Size: 1018 678 81 92 4072
Fragments arranged by size:
4072 1018 678 92 81
BbeI G_GCGC'C
Cuts at: 2889 2889
Size: 5941
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 3001 928
Size: 971 138 964 3868
Fragments arranged by size:
3868 971 964 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2267 2735 3833 5043 2267
Size: 468 1098 1210 3165
Fragments arranged by size:
3165 1210 1098 468
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2688 4552 136
Size: 107 122 71 631 605 1016 1864 1525
Fragments arranged by size:
1864 1525 1016 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2868 5936 2868
Size: 3068 2873
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2572 4002 5158 1790
Size: 782 1430 1156 2573
Fragments arranged by size:
2573 1430 1156 782
BmgBI CAC'GTC
Cuts at: 1318 2923 1318
Size: 1605 4336
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2598 2807 4601 1239
Size: 1359 209 1794 2579
Fragments arranged by size:
2579 1794 1359 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2246 4066 4931 5229 2228
Size: 18 1820 865 298 2940
Fragments arranged by size:
2940 1820 865 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2824 2914 3732 493
Size: 711 1620 90 818 2702
Fragments arranged by size:
2702 1620 818 711 90
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2597 2775 2886 3748
Size: 53 83 186 936 1064 178 111 862
Cuts at: 3748 4130 4765 275
Size: 382 635 1451
Fragments arranged by size:
1451 1064 936 862 635 382 186 178
111 83 53
BsaWI w'CCGG_w
Cuts at: 2310 4374 4772 5046 5193 5604 2310
Size: 2064 398 274 147 411 2647
Fragments arranged by size:
2647 2064 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2909 2939 1178
Size: 30 165 30 1506 30 4180
Fragments arranged by size:
4180 1506 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2861 3095 1186
Size: 1675 234 4032
Fragments arranged by size:
4032 1675 234
BseYI C'CCAG_C
Cuts at: 2394 2769 3161 5144 5640 2394
Size: 375 392 1983 496 2695
Fragments arranged by size:
2695 1983 496 392 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 2588 1163
Size: 573 645 207 4516
Fragments arranged by size:
4516 645 573 207
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2878 3391 4152 5180 1540
Size: 275 186 877 513 761 1028 2301
Fragments arranged by size:
2301 1028 877 761 513 275 186
BsiHKAI G_wGCw'C
Cuts at: 2222 2409 3087 3134 4002 4087 5158 2222
Size: 187 678 47 868 85 1071 3005
Fragments arranged by size:
3005 1071 868 678 187 85 47
BsmI GAATG_Cn'
Cuts at: 3206 3452 3545 3206
Size: 246 93 5602
Fragments arranged by size:
5602 246 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2222 2409 2572 3087 3134 3168 3260
Size: 399 432 187 163 515 47 34 92
Cuts at: 3260 4002 4087 5158 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 515 432 399 187 163
92 85 47 34
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2986 3000 3296 844
Size: 2142 14 296 3489
Fragments arranged by size:
3489 2142 296 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2857 3031 3828 1394
Size: 429 1034 174 797 3507
Fragments arranged by size:
3507 1034 797 429 174
BsrDI GCAATG_nn'
Cuts at: 65 1248 4436 4610 65
Size: 1183 3188 174 1396
Fragments arranged by size:
3188 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2310 4585 4772 1700
Size: 110 500 2275 187 2869
Fragments arranged by size:
2869 2275 500 187 110
BsrGI T'GTAC_A
Cuts at: 96 2487 2925 3000 96
Size: 2391 438 75 3037
Fragments arranged by size:
3037 2391 438 75
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2356
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 2163 2868 3271 3638 4023 4040 5492 5578
Size: 191 705 403 367 385 17 1452 86
Cuts at: 5578 5590 5936 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 705 403 385 367 346 191
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2231 2474 2931 3117 513
Size: 552 939 227 243 457 186 3337
Fragments arranged by size:
3337 939 552 457 243 227 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3028 487
Size: 683 56 1802 3400
Fragments arranged by size:
3400 1802 683 56
BtsI GCAGTG_nn'
Cuts at: 2600 3457 4251 4271 2600
Size: 857 794 20 4270
Fragments arranged by size:
4270 857 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2691 2726 529
Size: 35 2127 35 3744
Fragments arranged by size:
3744 2127 35 35
DraI TTT'AAA
Cuts at: 2019 3245 2019
Size: 1226 4715
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2135 4948 809
Size: 507 819 2813 1802
Fragments arranged by size:
2813 1802 819 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2346 3388
Size: 54 1425 50 275 18 249 267 1042
Cuts at: 3388 4277 5906 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1042 889 275 267 249 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3242 3871 1353
Size: 1889 629 3423
Fragments arranged by size:
3423 1889 629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4528 4902 5048 387
Size: 1064 138 435 2504 374 146 1280
Fragments arranged by size:
2504 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2407 3085 2407
Size: 678 5263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2598 2807 4004 4601 5388 5656 1239
Size: 393 966 209 1197 597 787 268 1524
Fragments arranged by size:
1524 1197 966 787 597 393 268 209
EcoNI CCTnn'n_nnAGG
Cuts at: 2528 2528
Size: 5941
EcoO109I rG'GnC_Cy
Cuts at: 2065 2065
Size: 5941
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
EcoRV GAT'ATC
Cuts at: 2204 2204
Size: 5941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 5941
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2387 2889 5088 1678
Size: 709 502 2199 2531
Fragments arranged by size:
2531 2199 709 502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2721 2753 4582 4614 4656
Size: 32 135 32 1149 32 1829 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 1829 1149 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2971 3285 3531 669
Size: 988 281 1033 314 246 3079
Fragments arranged by size:
3079 1033 988 314 281 246
HindIII A'AGCT_T
Cuts at: 748 2341 748
Size: 1593 4348
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2885 2885
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3171 5055 5239 1305
Size: 1866 1884 184 2007
Fragments arranged by size:
2007 1884 1866 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 2348 10
Size: 54 1425 343 249 267 3603
Fragments arranged by size:
3603 1425 343 267 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2979 3107 3137 3899 4258 4417 518
Size: 648 1813 128 30 762 359 159 2042
Fragments arranged by size:
2042 1813 762 648 359 159 128 30
MspA1I CmG'CkG
Cuts at: 1606 2059 2476 2679 2685 3357 4034 5182 5427
Size: 453 417 203 6 672 677 1148 245
Cuts at: 5427 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 672 453 417 245 203
6
NarI GG'CG_CC
Cuts at: 2886 2886
Size: 5941
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3106 3299 4844 1132
Size: 97 1877 193 1545 2229
Fragments arranged by size:
2229 1877 1545 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2065
Size: 5941
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2030 2064 2997 3293 830
Size: 1200 34 933 296 3478
Fragments arranged by size:
3478 1200 933 296 34
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2059 2059
Size: 5941
SacI G_AGCT'C
Cuts at: 2409 3087 2409
Size: 678 5263
SacII CC_GC'GG
Cuts at: 2477 2477
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SbfI CC_TGCA'GG
Cuts at: 2997 3293 2997
Size: 296 5645
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SexAI A'CCwGG_T
Cuts at: 2184 2355 2529 2184
Size: 171 174 5596
Fragments arranged by size:
5596 174 171
SfcI C'TryA_G
Cuts at: 826 1045 2026 2060 2442 2993 3289 4424 5105
Size: 219 981 34 382 551 296 1135 681
Cuts at: 5105 5296 826
Size: 191 1471
Fragments arranged by size:
1471 1135 981 681 551 382 296 219
191 34
SfoI GGC'GCC
Cuts at: 2887 2887
Size: 5941
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2592 3268 1872
Size: 720 676 4545
Fragments arranged by size:
4545 720 676
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 2261 4045 4946 5208 820
Size: 197 926 300 18 1784 901 262 1553
Fragments arranged by size:
1784 1553 926 901 300 262 197 18
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 2180 3865 5
Size: 47 2128 1685 2081
Fragments arranged by size:
2128 2081 1685 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 3380 513
Size: 552 939 1376 3074
Fragments arranged by size:
3074 1376 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2587 3968 3985 4138 4323 4768 325
Size: 1255 1007 1381 17 153 185 445 1498
Fragments arranged by size:
1498 1381 1255 1007 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 2925 3000
Size: 274 80 33 51 1443 510 438 75
Cuts at: 3000 4187 5899 96
Size: 1187 1712 138
Fragments arranged by size:
1712 1443 1187 510 438 274 138 80
75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 5901 5933 2549
Size: 32 3320 32 2557
Fragments arranged by size:
3320 2557 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3217 1316
Size: 1901 4040
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3154 3154
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2590 3266 1870
Size: 720 676 4545
Fragments arranged by size:
4545 720 676
XmnI GAAnn'nnTTC
Cuts at: 2807 4070 2807
Size: 1263 4678
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI