Restriction map for
pFN21AB6610
(Circular) (Six-base) MAPSORT of: pFN21AB6610.seq Check: 5146 from: 1 to: 5995
pFN21AB6610 5995 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 4122 4495 1563
Size: 2559 373 3063
Fragments arranged by size:
3063 2559 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 2394 4058 5442 5710 1632
Size: 716 46 1664 1384 268 1917
Fragments arranged by size:
1917 1664 1384 716 268 46
AfeI AGC'GCT
Cuts at: 2037 2672 2037
Size: 635 5360
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 4894 5871 1203
Size: 3691 977 1327
Fragments arranged by size:
3691 1327 977
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2778 3081 1184
Size: 1594 303 4098
Fragments arranged by size:
4098 1594 303
AlwNI CAG_nnn'CTG
Cuts at: 2541 2912 3738 5310 2541
Size: 371 826 1572 3226
Fragments arranged by size:
3226 1572 826 371
ApaI G_GGCC'C
Cuts at: 2338 2338
Size: 5995
ApaLI G'TGCA_C
Cuts at: 2768 4052 5208 2768
Size: 1284 1156 3555
Fragments arranged by size:
3555 1284 1156
ApoI r'AATT_y
Cuts at: 1493 2237 3304 3530 3682 5791 1493
Size: 744 1067 226 152 2109 1697
Fragments arranged by size:
2109 1697 1067 744 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4549 160
Size: 872 3517 1606
Fragments arranged by size:
3517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 2773 3185 3320 1870
Size: 73 830 412 135 4545
Fragments arranged by size:
4545 830 412 135 73
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2414 2956 3065 3316 4771
Size: 325 217 627 627 542 109 251 1455
Cuts at: 4771 618
Size: 1842
Fragments arranged by size:
1842 1455 627 627 542 325 251 217
109
BanII G_rGCy'C
Cuts at: 1391 2092 2175 2338 2483 2855 3111 3126 3288
Size: 701 83 163 145 372 256 15 162
Cuts at: 3288 3314 1391
Size: 26 4072
Fragments arranged by size:
4072 701 372 256 163 162 145 83
26 15
BbeI G_GCGC'C
Cuts at: 2960 2960
Size: 5995
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2501 928
Size: 971 602 4422
Fragments arranged by size:
4422 971 602
BbvCI CC'TCA_GC
Cuts at: 2832 2846 2946 2832
Size: 14 100 5881
Fragments arranged by size:
5881 100 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4186 4220 1903
Size: 34 2249 34 3678
Fragments arranged by size:
3678 2249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3887 5097 3887
Size: 1210 4785
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2603 3258
Size: 107 122 71 631 605 337 594 655
Cuts at: 3258 4606 136
Size: 1348 1525
Fragments arranged by size:
1525 1348 655 631 605 594 337 122
107 71
BglII A'GATC_T
Cuts at: 2935 3171 5990 2935
Size: 236 2819 2940
Fragments arranged by size:
2940 2819 236
BlpI GC'TnA_GC
Cuts at: 3412 3412
Size: 5995
Bme1580I G_kGCm'C
Cuts at: 1790 2338 2772 3133 3139 4056 5212 1790
Size: 548 434 361 6 917 1156 2573
Fragments arranged by size:
2573 1156 917 548 434 361 6
BmgBI CAC'GTC
Cuts at: 1318 2305 2458 2712 1318
Size: 987 153 254 4601
Fragments arranged by size:
4601 987 254 153
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2095 2468 3726
Size: 416 53 83 399 24 678 373 1258
Cuts at: 3726 4684 442
Size: 958 1753
Fragments arranged by size:
1753 1258 958 678 416 399 373 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2600 2639 2809 3000 4655 1239
Size: 935 426 39 170 191 1655 2579
Fragments arranged by size:
2579 1655 935 426 191 170 39
Bpu10I CC'TnA_GC
Cuts at: 2018 2832 2846 2946 2018
Size: 814 14 100 5067
Fragments arranged by size:
5067 814 100 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2905 4120 4985 5283 2905
Size: 1215 865 298 3617
Fragments arranged by size:
3617 1215 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2879 3020 4658 882
Size: 617 1380 141 1638 2219
Fragments arranged by size:
2219 1638 1380 617 141
BsaAI yAC'GTr
Cuts at: 493 1204 2053 3280 3786 493
Size: 711 849 1227 506 2702
Fragments arranged by size:
2702 1227 849 711 506
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2957 3802 4184 4819
Size: 53 83 186 936 1424 845 382 635
Cuts at: 4819 275
Size: 1451
Fragments arranged by size:
1451 1424 936 845 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4428 4826 5100 5247 5658 4428
Size: 398 274 147 411 4765
Fragments arranged by size:
4765 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2366 2396 2987 3017 1178
Size: 30 165 30 963 30 591 30 4156
Fragments arranged by size:
4156 963 591 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 2183 2378 2576 2579 2615 2863 2958
Size: 985 12 195 198 3 36 248 95
Cuts at: 2958 2963 3008 1186
Size: 5 45 4173
Fragments arranged by size:
4173 985 248 198 195 95 45 36
12 5 3
BseYI C'CCAG_C
Cuts at: 2960 5198 5694 2960
Size: 2238 496 3261
Fragments arranged by size:
3261 2238 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2640 3002 3074 1163
Size: 573 904 362 72 4084
Fragments arranged by size:
4084 904 573 362 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 3445 4206 5234 1540
Size: 275 186 81 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 2175 2772 3081 3288 4056 4141 5212 2175
Size: 597 309 207 768 85 1071 2958
Fragments arranged by size:
2958 1071 768 597 309 207 85
BsmI GAATG_Cn'
Cuts at: 3506 3599 3506
Size: 93 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2092 2175 2338 2483 2772 2855 3081
Size: 399 302 83 163 145 289 83 226
Cuts at: 3081 3111 3126 3133 3139 3288 3314 4056 4141
Size: 30 15 7 6 149 26 742 85
Cuts at: 4141 5212 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 302 289 226 163
149 145 85 83 83 30 26 15
7 6
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 3884 5614 3884
Size: 1730 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3350 844
Size: 2506 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3882 1394
Size: 429 2059 3507
Fragments arranged by size:
3507 2059 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2122 4490 4664 65
Size: 1183 874 2368 174 1396
Fragments arranged by size:
2368 1396 1183 874 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2953 3068 4639 4826 1700
Size: 110 1143 115 1571 187 2869
Fragments arranged by size:
2869 1571 1143 187 115 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5995
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5995
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 2062 1184
Size: 878 5117
BstXI CCAn_nnnn'nTGG
Cuts at: 3280 3280
Size: 5995
BstYI r'GATC_y
Cuts at: 1972 2935 3171 3325 3692 4077 4094 5546 5632
Size: 963 236 154 367 385 17 1452 86
Cuts at: 5632 5644 5990 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 963 385 367 346 236 154
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2313 513
Size: 552 939 309 4195
Fragments arranged by size:
4195 939 552 309
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5256
Fragments arranged by size:
5256 683 56
BtsI GCAGTG_nn'
Cuts at: 3511 4305 4325 3511
Size: 794 20 5181
Fragments arranged by size:
5181 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2422 2457 529
Size: 35 1858 35 4067
Fragments arranged by size:
4067 1858 35 35
DraI TTT'AAA
Cuts at: 3299 3299
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5002 809
Size: 507 3686 1802
Fragments arranged by size:
3686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 2400 2443
Size: 54 1425 50 275 18 486 84 43
Cuts at: 2443 3250 3442 4331 5960 8
Size: 807 192 889 1629 43
Fragments arranged by size:
1629 1425 889 807 486 275 192 84
54 50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 1537
Size: 275 1630 4090
Fragments arranged by size:
4090 1630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2985 3925 1353
Size: 1632 940 3423
Fragments arranged by size:
3423 1632 940
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4582 4956 5102 387
Size: 1064 138 2993 374 146 1280
Fragments arranged by size:
2993 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2173 3286 2173
Size: 1113 4882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2348 2394 2600 2639 2809 3000
Size: 393 542 174 46 206 39 170 191
Cuts at: 3000 4058 4655 5442 5710 1239
Size: 1058 597 787 268 1524
Fragments arranged by size:
1524 1058 787 597 542 393 268 206
191 174 170 46 39
EcoO109I rG'GnC_Cy
Cuts at: 2215 2334 2462 2470 2215
Size: 119 128 8 5740
Fragments arranged by size:
5740 128 119 8
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 2039 2632 2674 2960 5142 1678
Size: 361 593 42 286 2182 2531
Fragments arranged by size:
2531 2182 593 361 286 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 2744 2776 4636
Size: 32 135 32 635 32 505 32 1860
Cuts at: 4636 4668 4710 4742 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1860 635 505 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3339 3585 669
Size: 988 281 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5995
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KasI G'GCGC_C
Cuts at: 2956 2956
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5109 5293 1305
Size: 3804 184 2007
Fragments arranged by size:
3804 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2318 2402 3252 10
Size: 54 1425 343 486 84 850 2753
Fragments arranged by size:
2753 1425 850 486 343 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 3209 3953 4312 4471 518
Size: 648 960 1083 744 359 159 2042
Fragments arranged by size:
2042 1083 960 744 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2181 2611 2617 2909 3411 4088 5236 5481
Size: 575 430 6 292 502 677 1148 245
Cuts at: 5481 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 575 502 430 292 245
6
NaeI GCC'GGC
Cuts at: 2955 3070 2955
Size: 115 5880
NarI GG'CG_CC
Cuts at: 2957 2957
Size: 5995
NcoI C'CATG_G
Cuts at: 513 1065 2004 2313 513
Size: 552 939 309 4195
Fragments arranged by size:
4195 939 552 309
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NgoMIV G'CCGG_C
Cuts at: 2953 3068 2953
Size: 115 5880
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 3265 3353 4898 1132
Size: 97 2036 88 1545 2229
Fragments arranged by size:
2229 2036 1545 97 88
PasI CC'CwG_GG
Cuts at: 1348 2928 1348
Size: 1580 4415
PciI A'CATG_T
Cuts at: 4894 4894
Size: 5995
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2458 1108
Size: 1350 4645
PfoI T'CCnGG_A
Cuts at: 2451 2451
Size: 5995
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2215 2462 2215
Size: 247 5748
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5995
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PspOMI G'GGCC_C
Cuts at: 2334 2334
Size: 5995
PstI C_TGCA'G
Cuts at: 830 2207 2865 3347 830
Size: 1377 658 482 3478
Fragments arranged by size:
3478 1377 658 482
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 2617 2909 2617
Size: 292 5703
SacI G_AGCT'C
Cuts at: 2175 3288 2175
Size: 1113 4882
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SanDI GG'GwC_CC
Cuts at: 2462 2462
Size: 5995
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SfcI C'TryA_G
Cuts at: 826 1045 2203 2422 2861 3343 4478 5159 5350
Size: 219 1158 219 439 482 1135 681 191
Cuts at: 5350 826
Size: 1471
Fragments arranged by size:
1471 1158 1135 681 482 439 219 219
191
SfoI GGC'GCC
Cuts at: 2958 2958
Size: 5995
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 3322 1872
Size: 1450 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2920 4099 5000 5262 820
Size: 197 926 977 1179 901 262 1553
Fragments arranged by size:
1553 1179 977 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 3919 5
Size: 47 3867 2081
Fragments arranged by size:
3867 2081 47
StuI AGG'CCT
Cuts at: 1517 2048 1517
Size: 531 5464
StyI C'CwwG_G
Cuts at: 513 1065 2004 2313 2403 3434 513
Size: 552 939 309 90 1031 3074
Fragments arranged by size:
3074 1031 939 552 309 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2096 2891 4022 4039 4192 4377 4822
Size: 1255 516 795 1131 17 153 185 445
Cuts at: 4822 325
Size: 1498
Fragments arranged by size:
1498 1255 1131 795 516 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4241 5953 96
Size: 274 80 33 51 1443 2264 1712 138
Fragments arranged by size:
2264 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2366 2398 5955 5987 2366
Size: 32 3557 32 2374
Fragments arranged by size:
3557 2374 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3331 3331
Size: 5995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3110 3110
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 3320 1870
Size: 1450 4545
XmnI GAAnn'nnTTC
Cuts at: 4124 4124
Size: 5995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SapI SexAI SfiI SrfI SwaI