Restriction map for
pFN21AB6608
(Circular) (Six-base) MAPSORT of: pFN21AB6608.seq Check: 5805 from: 1 to: 5998
pFN21AB6608 5998 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2401 2853 2401
Size: 452 5546
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2715 3228 3341 1937
Size: 778 513 113 4594
Fragments arranged by size:
4594 778 513 113
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 2324 2861 3084 3230 4061 5445 5713
Size: 656 36 537 223 146 831 1384 268
Cuts at: 5713 1632
Size: 1917
Fragments arranged by size:
1917 1384 831 656 537 268 223 146
36
AfeI AGC'GCT
Cuts at: 2803 2803
Size: 5998
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 2117 2625 4897 5874 1203
Size: 914 508 2272 977 1327
Fragments arranged by size:
2272 1327 977 914 508
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 3143 4727 3143
Size: 1584 4414
AleI CACnn'nnGTG
Cuts at: 1166 2624 1166
Size: 1458 4540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AlwNI CAG_nnn'CTG
Cuts at: 3741 5313 3741
Size: 1572 4426
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 2171 2498 2552 2968 3307 3533 3685 5794
Size: 678 327 54 416 339 226 152 2109
Cuts at: 5794 1493
Size: 1697
Fragments arranged by size:
2109 1697 678 416 339 327 226 152
54
AscI GG'CGCG_CC
Cuts at: 2021 2021
Size: 5998
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 3323 1870
Size: 73 1380 4545
Fragments arranged by size:
4545 1380 73
BamHI G'GATC_C
Cuts at: 3258 3328 3695 3258
Size: 70 367 5561
Fragments arranged by size:
5561 367 70
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2601 3319 4774 618
Size: 325 217 627 814 718 1455 1842
Fragments arranged by size:
1842 1455 814 718 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2014 2615 3317 1391
Size: 623 601 702 4072
Fragments arranged by size:
4072 702 623 601
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2382 2416 4189 4223 1903
Size: 34 445 34 1773 34 3678
Fragments arranged by size:
3678 1773 445 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 2661 1548
Size: 1113 4885
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2018 4609 136
Size: 107 122 71 631 605 346 2591 1525
Fragments arranged by size:
2591 1525 631 605 346 122 107 71
BglII A'GATC_T
Cuts at: 2327 5993 2327
Size: 3666 2332
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 3208 4059 5215 1790
Size: 1418 851 1156 2573
Fragments arranged by size:
2573 1418 1156 851
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2920 3729 4687
Size: 416 53 83 399 24 1503 809 958
Cuts at: 4687 442
Size: 1753
Fragments arranged by size:
1753 1503 958 809 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2657 2657
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2469 4658 1239
Size: 1230 2189 2579
Fragments arranged by size:
2579 2189 1230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2713 4123 4988 5286 2713
Size: 1410 865 298 3425
Fragments arranged by size:
3425 1410 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2626 3789 493
Size: 711 1422 1163 2702
Fragments arranged by size:
2702 1422 1163 711
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3225 3805 4187 4822
Size: 53 83 186 936 1692 580 382 635
Cuts at: 4822 275
Size: 1451
Fragments arranged by size:
1692 1451 936 635 580 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2385 2989 4431 4829 5103 5250 5661 2385
Size: 604 1442 398 274 147 411 2722
Fragments arranged by size:
2722 1442 604 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2747 2777 2849 2879 1178
Size: 30 165 30 1344 30 72 30 4297
Fragments arranged by size:
4297 1344 165 72 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2467 2472 1186
Size: 1281 5 4712
Fragments arranged by size:
4712 1281 5
BseYI C'CCAG_C
Cuts at: 2344 2655 3189 5201 5697 2344
Size: 311 534 2012 496 2645
Fragments arranged by size:
2645 2012 534 496 311
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 1163
Size: 573 693 4732
Fragments arranged by size:
4732 693 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4059 4144 5215 4059
Size: 85 1071 4842
Fragments arranged by size:
4842 1071 85
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2615 3208 3317 4059 4144 5215
Size: 399 224 601 593 109 742 85 1071
Cuts at: 5215 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 601 593 399 224 109
85
BspEI T'CCGG_A
Cuts at: 2989 5661 2989
Size: 2672 3326
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2219 2401 2853 3353 844
Size: 1375 182 452 500 3489
Fragments arranged by size:
3489 1375 500 452 182
BsrBI CCG'CTC
Cuts at: 1394 1823 3885 1394
Size: 429 2062 3507
Fragments arranged by size:
3507 2062 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2074 2829 4493 4667 65
Size: 1183 826 755 1664 174 1396
Fragments arranged by size:
1664 1396 1183 826 755 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2268 4642 4829 1700
Size: 110 458 2374 187 2869
Fragments arranged by size:
2869 2374 458 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5998
BssHII G'CGCG_C
Cuts at: 1925 2021 1925
Size: 96 5902
BssSI C'ACGA_G
Cuts at: 3232 4058 5070 3232
Size: 826 1012 4160
Fragments arranged by size:
4160 1012 826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5998
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstYI r'GATC_y
Cuts at: 1972 2327 2844 3258 3328 3695 4080 4097 5549
Size: 355 517 414 70 367 385 17 1452
Cuts at: 5549 5635 5647 5993 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 517 414 385 367 355 346
86 70 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2262 3196 513
Size: 552 1197 934 3315
Fragments arranged by size:
3315 1197 934 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 2674 3514 4308 4328 2674
Size: 840 794 20 4344
Fragments arranged by size:
4344 840 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2711 5005 809
Size: 507 1395 2294 1802
Fragments arranged by size:
2294 1802 1395 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2509 2586 3445
Size: 54 1425 50 275 18 679 77 859
Cuts at: 3445 4334 5963 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 859 679 275 77 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2343 3273 3928 1353
Size: 990 930 655 3423
Fragments arranged by size:
3423 990 930 655
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4585 4959 5105 387
Size: 1064 138 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2324 2469 2861 3084 3230 4061
Size: 393 656 36 145 392 223 146 831
Cuts at: 4061 4658 5445 5713 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 831 787 656 597 393 392 268
223 146 145 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2789 2789
Size: 5998
EcoO109I rG'GnC_Cy
Cuts at: 2526 3137 2526
Size: 611 5387
EcoRI G'AATT_C
Cuts at: 2552 2968 3307 3685 2552
Size: 416 339 378 4865
Fragments arranged by size:
4865 416 378 339
FseI GG_CCGG'CC
Cuts at: 2272 2272
Size: 5998
FspI TGC'GCA
Cuts at: 2458 2871 4504 2458
Size: 413 1633 3952
Fragments arranged by size:
3952 1633 413
HaeII r_GCGC'y
Cuts at: 1678 2670 2805 5145 1678
Size: 992 135 2340 2531
Fragments arranged by size:
2531 2340 992 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3129 3161 3250 3282 4639
Size: 32 135 32 1557 32 89 32 1357
Cuts at: 4639 4671 4713 4745 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1557 1357 135 89 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2151 2166 2766 5112 5296 1305
Size: 846 15 600 2346 184 2007
Fragments arranged by size:
2346 2007 846 600 184 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2511 10
Size: 54 1425 343 679 3497
Fragments arranged by size:
3497 1425 679 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 3077 3956 4315 4474 518
Size: 648 1458 453 879 359 159 2042
Fragments arranged by size:
2042 1458 879 648 453 359 159
MspA1I CmG'CkG
Cuts at: 1606 2344 2998 3414 4091 5239 5484 1606
Size: 738 654 416 677 1148 245 2120
Fragments arranged by size:
2120 1148 738 677 654 416 245
NaeI GCC'GGC
Cuts at: 2270 2270
Size: 5998
NcoI C'CATG_G
Cuts at: 513 1065 2262 513
Size: 552 1197 4249
Fragments arranged by size:
4249 1197 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NgoMIV G'CCGG_C
Cuts at: 2268 2268
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 2121 3356 4901 1132
Size: 97 892 1235 1545 2229
Fragments arranged by size:
2229 1545 1235 892 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5998
PciI A'CATG_T
Cuts at: 2117 4897 2117
Size: 2780 3218
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5998
PfoI T'CCnGG_A
Cuts at: 2879 2879
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3137 3137
Size: 5998
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PstI C_TGCA'G
Cuts at: 830 2372 3350 830
Size: 1542 978 3478
Fragments arranged by size:
3478 1542 978
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2344 2998 2344
Size: 654 5344
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SanDI GG'GwC_CC
Cuts at: 3137 3137
Size: 5998
SapI GCTCTTCn'nnn_
Cuts at: 2343 2343
Size: 5998
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 2135 4246 5958 2135
Size: 2111 1712 2175
Fragments arranged by size:
2175 2111 1712
SfcI C'TryA_G
Cuts at: 826 1045 2368 2593 2797 3346 4481 5162 5353
Size: 219 1323 225 204 549 1135 681 191
Cuts at: 5353 826
Size: 1471
Fragments arranged by size:
1471 1323 1135 681 549 225 219 204
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3325 1872
Size: 1453 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2728 4102 5003 5265 820
Size: 197 926 785 1374 901 262 1553
Fragments arranged by size:
1553 1374 926 901 785 262 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2262 2853 3157 3273 3437 513
Size: 552 1197 591 304 116 164 3074
Fragments arranged by size:
3074 1197 591 552 304 164 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 4025 4042 4195 4380 4825 325
Size: 1255 1067 1378 17 153 185 445 1498
Fragments arranged by size:
1498 1378 1255 1067 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 4244 5956
Size: 274 80 33 51 1443 156 2111 1712
Cuts at: 5956 96
Size: 138
Fragments arranged by size:
2111 1712 1443 274 156 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2106 2138 2849 2881 5958 5990 2106
Size: 32 711 32 3077 32 2114
Fragments arranged by size:
3077 2114 711 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 3323 1870
Size: 1453 4545
XmnI GAAnn'nnTTC
Cuts at: 4127 4127
Size: 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI XcmI