Restriction map for
pFN21AB6607
(Circular) (Six-base) MAPSORT of: pFN21AB6607.seq Check: 5146 from: 1 to: 5932
pFN21AB6607 5932 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2380 2428 2380
Size: 48 5884
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3742 278
Size: 53 83 186 936 2206 2468
Fragments arranged by size:
2468 2206 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3275 1937
Size: 1338 4594
Acc65I G'GTAC_C
Cuts at: 3253 3253
Size: 5932
AclI AA'CG_TT
Cuts at: 1563 4059 4432 1563
Size: 2496 373 3063
Fragments arranged by size:
3063 2496 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2745 2930 3162 3995 5379 5647 1632
Size: 407 706 185 232 833 1384 268 1917
Fragments arranged by size:
1917 1384 833 706 407 268 232 185
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5735
AflIII A'CryG_T
Cuts at: 1203 4831 5808 1203
Size: 3628 977 1327
Fragments arranged by size:
3628 1327 977
AgeI A'CCGG_T
Cuts at: 4763 4763
Size: 5932
AhdI GACnn_n'nnGTC
Cuts at: 4661 4661
Size: 5932
AleI CACnn'nnGTG
Cuts at: 1166 2321 1166
Size: 1155 4777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2643 1184
Size: 1459 4473
AlwNI CAG_nnn'CTG
Cuts at: 2282 2495 2771 3059 3675 5247 2282
Size: 213 276 288 616 1572 2967
Fragments arranged by size:
2967 1572 616 288 276 213
ApaLI G'TGCA_C
Cuts at: 2550 3989 5145 2550
Size: 1439 1156 3337
Fragments arranged by size:
3337 1439 1156
ApoI r'AATT_y
Cuts at: 1493 2834 3187 3241 3467 3619 5728 1493
Size: 1341 353 54 226 152 2109 1697
Fragments arranged by size:
2109 1697 1341 353 226 152 54
AseI AT'TA_AT
Cuts at: 160 1032 2141 4486 160
Size: 872 1109 2345 1606
Fragments arranged by size:
2345 1606 1109 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 3257 1870
Size: 73 117 1197 4545
Fragments arranged by size:
4545 1197 117 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3146 3179 3146
Size: 33 5899
BamHI G'GATC_C
Cuts at: 3262 3629 3262
Size: 367 5565
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3253 4708 618
Size: 325 217 627 1466 1455 1842
Fragments arranged by size:
1842 1466 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2752 3251 1391
Size: 1361 499 4072
Fragments arranged by size:
4072 1361 499
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2027 2751 928
Size: 971 128 724 4109
Fragments arranged by size:
4109 971 724 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2906 2940 4123 4157 1903
Size: 34 50 34 885 34 1183 34 3678
Fragments arranged by size:
3678 1183 885 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3824 5034 3824
Size: 1210 4722
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5932
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4543 136
Size: 107 122 71 631 605 2871 1525
Fragments arranged by size:
2871 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5927 5927
Size: 5932
BlpI GC'TnA_GC
Cuts at: 3349 3349
Size: 5932
Bme1580I G_kGCm'C
Cuts at: 1790 2554 3993 5149 1790
Size: 764 1439 1156 2573
Fragments arranged by size:
2573 1439 1156 764
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5932
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2754 3663 4621
Size: 416 53 83 399 24 1337 909 958
Cuts at: 4621 442
Size: 1753
Fragments arranged by size:
1753 1337 958 909 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2012 2370 2715 2838 4592 1239
Size: 773 358 345 123 1754 2579
Fragments arranged by size:
2579 1754 773 358 345 123
Bpu10I CC'TnA_GC
Cuts at: 2328 2328
Size: 5932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2715 4057 4922 5220 2715
Size: 1342 865 298 3427
Fragments arranged by size:
3427 1342 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4595 882
Size: 617 3096 2219
Fragments arranged by size:
3096 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3723 493
Size: 711 2519 2702
Fragments arranged by size:
2702 2519 711
BsaBI GATnn'nnATC
Cuts at: 5926 5926
Size: 5932
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3739 4121 4756 275
Size: 53 83 186 936 2206 382 635 1451
Fragments arranged by size:
2206 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4365 4763 5037 5184 5595 4365
Size: 398 274 147 411 4702
Fragments arranged by size:
4702 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2734 2764 1178
Size: 30 165 30 1331 30 4346
Fragments arranged by size:
4346 1331 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2742 3036 3039 1186
Size: 892 664 294 3 4079
Fragments arranged by size:
4079 892 664 294 3
BseYI C'CCAG_C
Cuts at: 5135 5631 5135
Size: 496 5436
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2400 2448 1163
Size: 573 664 48 4647
Fragments arranged by size:
4647 664 573 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3382 4143 5171 1540
Size: 275 186 1381 761 1028 2301
Fragments arranged by size:
2301 1381 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2554 3993 4078 5149 2554
Size: 1439 85 1071 3337
Fragments arranged by size:
3337 1439 1071 85
BsmI GAATG_Cn'
Cuts at: 3443 3536 3443
Size: 93 5839
BsmBI CGTCTCn'nnnn_
Cuts at: 2060 2060
Size: 5932
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2554 2752 3251 3993 4078 5149 1391
Size: 399 764 198 499 742 85 1071 2174
Fragments arranged by size:
2174 1071 764 742 499 399 198 85
BspEI T'CCGG_A
Cuts at: 5595 5595
Size: 5932
BspHI T'CATG_A
Cuts at: 3821 5551 3821
Size: 1730 4202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1999 2120 2380 2422 2428 3287 844
Size: 1155 121 260 42 6 859 3489
Fragments arranged by size:
3489 1155 859 260 121 42 6
BsrBI CCG'CTC
Cuts at: 1394 1823 3819 1394
Size: 429 1996 3507
Fragments arranged by size:
3507 1996 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4427 4601 65
Size: 1183 3179 174 1396
Fragments arranged by size:
3179 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4576 4763 1700
Size: 110 2766 187 2869
Fragments arranged by size:
2869 2766 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5932
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5932
BssSI C'ACGA_G
Cuts at: 3992 5004 3992
Size: 1012 4920
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2549 2771 794
Size: 1755 222 3955
Fragments arranged by size:
3955 1755 222
BstBI TT'CG_AA
Cuts at: 4736 4736
Size: 5932
BstEII G'GTnAC_C
Cuts at: 1184 2011 2224 1184
Size: 827 213 4892
Fragments arranged by size:
4892 827 213
BstXI CCAn_nnnn'nTGG
Cuts at: 2323 2323
Size: 5932
BstYI r'GATC_y
Cuts at: 1972 3262 3629 4014 4031 5483 5569 5581 5927
Size: 1290 367 385 17 1452 86 12 346
Cuts at: 5927 1972
Size: 1977
Fragments arranged by size:
1977 1452 1290 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3096 3096
Size: 5932
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5380
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5193
Fragments arranged by size:
5193 683 56
BtsI GCAGTG_nn'
Cuts at: 2247 2673 2772 3046 3448 4242 4262 2247
Size: 426 99 274 402 794 20 3917
Fragments arranged by size:
3917 794 426 402 274 99 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5932
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5897
DraI TTT'AAA
Cuts at: 3236 3236
Size: 5932
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2566 4939 809
Size: 507 1250 2373 1802
Fragments arranged by size:
2373 1802 1250 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3379 4268 5897
Size: 54 1425 50 275 18 1549 889 1629
Cuts at: 5897 8
Size: 43
Fragments arranged by size:
1629 1549 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3379 1537
Size: 275 1567 4090
Fragments arranged by size:
4090 1567 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3862 1353
Size: 2509 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4519 4893 5039 387
Size: 1064 138 2930 374 146 1280
Fragments arranged by size:
2930 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 2039 2370 2715 2745 2838 2930
Size: 393 380 27 331 345 30 93 92
Cuts at: 2930 3162 3995 4592 5379 5647 1239
Size: 232 833 597 787 268 1524
Fragments arranged by size:
1524 833 787 597 393 380 345 331
268 232 93 92 30 27
EcoRI G'AATT_C
Cuts at: 3241 3619 3241
Size: 378 5554
FspI TGC'GCA
Cuts at: 4438 4438
Size: 5932
HaeII r_GCGC'y
Cuts at: 1678 5079 1678
Size: 3401 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2489 2521 2582 2614 4573
Size: 32 135 32 917 32 61 32 1959
Cuts at: 4573 4605 4647 4679 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1959 917 135 61 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2722 3276 3522 669
Size: 988 281 784 554 246 3079
Fragments arranged by size:
3079 988 784 554 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5932
HpaI GTT'AAC
Cuts at: 3522 3522
Size: 5932
KpnI G_GTAC'C
Cuts at: 3257 3257
Size: 5932
MfeI C'AATT_G
Cuts at: 3531 3531
Size: 5932
MluI A'CGCG_T
Cuts at: 5808 5808
Size: 5932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2360 5046 5230 1305
Size: 1055 2686 184 2007
Fragments arranged by size:
2686 2007 1055 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4110
Fragments arranged by size:
4110 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 2600 3890 4249 4408 518
Size: 648 1155 279 1290 359 159 2042
Fragments arranged by size:
2042 1290 1155 648 359 279 159
MspA1I CmG'CkG
Cuts at: 1606 2777 3348 4025 5173 5418 1606
Size: 1171 571 677 1148 245 2120
Fragments arranged by size:
2120 1171 1148 677 571 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5380
NdeI CA'TA_TG
Cuts at: 387 3072 387
Size: 2685 3247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5932
NotI GC'GGCC_GC
Cuts at: 3379 3379
Size: 5932
NspI r_CATG'y
Cuts at: 1132 1229 3290 4835 1132
Size: 97 2061 1545 2229
Fragments arranged by size:
2229 2061 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5932
PciI A'CATG_T
Cuts at: 4831 4831
Size: 5932
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5932
PmeI GTTT'AAAC
Cuts at: 3236 3236
Size: 5932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4001 4033 5514 5546 4001
Size: 32 1481 32 4387
Fragments arranged by size:
4387 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5932
PsiI TTA'TAA
Cuts at: 3502 3502
Size: 5932
PstI C_TGCA'G
Cuts at: 830 2452 2776 2782 3284 830
Size: 1622 324 6 502 3478
Fragments arranged by size:
3478 1622 502 324 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5932
PvuII CAG'CTG
Cuts at: 2777 2777
Size: 5932
SalI G'TCGA_C
Cuts at: 1936 3274 1936
Size: 1338 4594
SbfI CC_TGCA'GG
Cuts at: 3284 3284
Size: 5932
ScaI AGT'ACT
Cuts at: 2743 4180 5892 2743
Size: 1437 1712 2783
Fragments arranged by size:
2783 1712 1437
SfcI C'TryA_G
Cuts at: 826 1045 2292 2448 2772 2778 2912 3111 3209
Size: 219 1247 156 324 6 134 199 98
Cuts at: 3209 3280 4415 5096 5287 826
Size: 71 1135 681 191 1471
Fragments arranged by size:
1471 1247 1135 681 324 219 199 191
156 134 98 71 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5932
SmaI CCC'GGG
Cuts at: 1872 3259 1872
Size: 1387 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2730 4036 4937 5199 820
Size: 197 926 787 1306 901 262 1553
Fragments arranged by size:
1553 1306 926 901 787 262 197
SnaBI TAC'GTA
Cuts at: 493 3723 493
Size: 3230 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5932
SphI G_CATG'C
Cuts at: 1132 3290 1132
Size: 2158 3774
SspI AAT'ATT
Cuts at: 5 52 3856 5
Size: 47 3804 2081
Fragments arranged by size:
3804 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5932
StyI C'CwwG_G
Cuts at: 513 1065 2352 3371 513
Size: 552 1287 1019 3074
Fragments arranged by size:
3074 1287 1019 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2511 3959 3976 4129 4314 4759 325
Size: 1255 931 1448 17 153 185 445 1498
Fragments arranged by size:
1498 1448 1255 931 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2501 2741 4178
Size: 274 80 33 51 1443 524 240 1437
Cuts at: 4178 5890 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1437 524 274 240 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5892 5924 5892
Size: 32 5900
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5932
XbaI T'CTAG_A
Cuts at: 3268 3268
Size: 5932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 2320
Size: 5932
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5932
XmaI C'CCGG_G
Cuts at: 1870 3257 1870
Size: 1387 4545
XmnI GAAnn'nnTTC
Cuts at: 2997 4061 2997
Size: 1064 4868
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3740 276
Size: 53 83 186 936 2206 2468
Fragments arranged by size:
2468 2206 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI