Restriction map for
pFN21AB6606
(Circular) (Six-base) MAPSORT of: pFN21AB6606.seq Check: 344 from: 1 to: 5998
pFN21AB6606 5998 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2072 2072
Size: 5998
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2624 4061 5445 5713 1632
Size: 992 1437 1384 268 1917
Fragments arranged by size:
1917 1437 1384 992 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 4727 4727
Size: 5998
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2307 1184
Size: 1123 4875
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2528 2560 2528
Size: 32 5966
AlwNI CAG_nnn'CTG
Cuts at: 2164 3133 3741 5313 2164
Size: 969 608 1572 2849
Fragments arranged by size:
2849 1572 969 608
ApaI G_GGCC'C
Cuts at: 2873 2873
Size: 5998
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 2288 3307 3533 3685 5794 1493
Size: 795 1019 226 152 2109 1697
Fragments arranged by size:
2109 1697 1019 795 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2118 2652 3195 3242 3323 1870
Size: 73 91 84 534 543 47 81 4545
Fragments arranged by size:
4545 543 534 91 84 81 73 47
BamHI G'GATC_C
Cuts at: 3123 3328 3695 3123
Size: 205 367 5426
Fragments arranged by size:
5426 367 205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2212 2239 3319 4774
Size: 325 217 627 273 152 27 1080 1455
Cuts at: 4774 618
Size: 1842
Fragments arranged by size:
1842 1455 1080 627 325 273 217 152
27
BanII G_rGCy'C
Cuts at: 1391 2873 3204 3317 1391
Size: 1482 331 113 4072
Fragments arranged by size:
4072 1482 331 113
BbeI G_GCGC'C
Cuts at: 2243 2243
Size: 5998
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2416 3064 3166 3267 928
Size: 971 517 648 102 101 3659
Fragments arranged by size:
3659 971 648 517 102 101
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2254 2288 4189 4223 1903
Size: 34 317 34 1901 34 3678
Fragments arranged by size:
3678 1901 317 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2831 4609 136
Size: 107 122 71 631 605 1159 1778 1525
Fragments arranged by size:
1778 1525 1159 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2559 5993 2559
Size: 3434 2564
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 2217 2652 2873 4059 5215 1790
Size: 427 435 221 1186 1156 2573
Fragments arranged by size:
2573 1186 1156 435 427 221
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2639 3729 4687
Size: 416 53 83 399 24 1222 1090 958
Cuts at: 4687 442
Size: 1753
Fragments arranged by size:
1753 1222 1090 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 3026 3188 3245 4658 1239
Size: 1280 507 162 57 1413 2579
Fragments arranged by size:
2579 1413 1280 507 162 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2573 4123 4988 5286 2573
Size: 1550 865 298 3285
Fragments arranged by size:
3285 1550 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3134 4661 882
Size: 617 1635 1527 2219
Fragments arranged by size:
2219 1635 1527 617
BsaAI yAC'GTr
Cuts at: 493 1204 3789 493
Size: 711 2585 2702
Fragments arranged by size:
2702 2585 711
BsaBI GATnn'nnATC
Cuts at: 2558 5992 2558
Size: 3434 2564
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2240 2963 3805 4187
Size: 53 83 186 936 707 723 842 382
Cuts at: 4187 4822 275
Size: 635 1451
Fragments arranged by size:
1451 936 842 723 707 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2179 4431 4829 5103 5250 5661 2179
Size: 2252 398 274 147 411 2516
Fragments arranged by size:
2516 2252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2528 2558 1178
Size: 30 165 30 1125 30 4618
Fragments arranged by size:
4618 1125 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2957 2984 3031 3242 1186
Size: 1771 27 47 211 3942
Fragments arranged by size:
3942 1771 211 47 27
BseYI C'CCAG_C
Cuts at: 2010 2171 2784 3024 5201 5697 2010
Size: 161 613 240 2177 496 2311
Fragments arranged by size:
2311 2177 613 496 240 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2303 2790 1163
Size: 573 567 487 4371
Fragments arranged by size:
4371 573 567 487
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2301 3097 3204 4059 4144 5215 2301
Size: 796 107 855 85 1071 3084
Fragments arranged by size:
3084 1071 855 796 107 85
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
BsmBI CGTCTCn'nnnn_
Cuts at: 2531 2954 2531
Size: 423 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2301 2652 2873 3097 3204 3317
Size: 399 427 84 351 221 224 107 113
Cuts at: 3317 4059 4144 5215 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 399 351 224 221
113 107 85 84
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 2625 3097 3887 5617 2625
Size: 472 790 1730 3006
Fragments arranged by size:
3006 1730 790 472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2072 2667 3279 3353 844
Size: 1228 595 612 74 3489
Fragments arranged by size:
3489 1228 612 595 74
BsrBI CCG'CTC
Cuts at: 1394 1823 2374 3885 1394
Size: 429 551 1511 3507
Fragments arranged by size:
3507 1511 551 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4493 4667 65
Size: 1183 3245 174 1396
Fragments arranged by size:
3245 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 4642 4829 1700
Size: 110 299 2533 187 2869
Fragments arranged by size:
2869 2533 299 187 110
BsrGI T'GTAC_A
Cuts at: 96 2388 96
Size: 2292 3706
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 4058 5070 4058
Size: 1012 4986
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2281 2305 794
Size: 1487 24 4487
Fragments arranged by size:
4487 1487 24
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 2728 1184
Size: 1544 4454
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2209 3085 2029
Size: 180 876 4942
Fragments arranged by size:
4942 876 180
BstYI r'GATC_y
Cuts at: 1972 2559 2776 3123 3162 3328 3695 4080 4097
Size: 587 217 347 39 166 367 385 17
Cuts at: 4097 5549 5635 5647 5993 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 587 385 367 347 346 217
166 86 39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2515 3168 513
Size: 552 1450 653 3343
Fragments arranged by size:
3343 1450 653 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 2128 3514 4308 4328 2128
Size: 1386 794 20 3798
Fragments arranged by size:
3798 1386 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DraIII CAC_nnn'GTG
Cuts at: 2305 2521 2305
Size: 216 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2682 3445 4334
Size: 54 1425 50 275 18 852 763 889
Cuts at: 4334 5963 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 852 763 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2952 3928 1353
Size: 1599 976 3423
Fragments arranged by size:
3423 1599 976
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3167 4585 4959 5105 387
Size: 1064 138 1578 1418 374 146 1280
Fragments arranged by size:
1578 1418 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3202 3202
Size: 5998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2624 3026 3188 3245 4061 4658
Size: 393 887 105 402 162 57 816 597
Cuts at: 4658 5445 5713 1239
Size: 787 268 1524
Fragments arranged by size:
1524 887 816 787 597 402 393 268
162 105 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2663 3242 2663
Size: 579 5419
EcoO109I rG'GnC_Cy
Cuts at: 2049 2101 2122 2224 2377 2656 2668 2869 2870
Size: 52 21 102 153 279 12 201 1
Cuts at: 2870 3065 3130 2049
Size: 195 65 4917
Fragments arranged by size:
4917 279 201 195 153 102 65 52
21 12 1
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 2440 2284
Size: 156 5842
FspI TGC'GCA
Cuts at: 2348 4504 2348
Size: 2156 3842
HaeII r_GCGC'y
Cuts at: 1678 2243 5145 1678
Size: 565 2902 2531
Fragments arranged by size:
2902 2531 565
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2800 2832 4639 4671 4713
Size: 32 135 32 1228 32 1807 32 42
Cuts at: 4713 4745 1373
Size: 32 2626
Fragments arranged by size:
2626 1807 1228 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2635 3342 3588 669
Size: 988 281 697 707 246 3079
Fragments arranged by size:
3079 988 707 697 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KasI G'GCGC_C
Cuts at: 2239 2239
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5112 5296 1305
Size: 3807 184 2007
Fragments arranged by size:
3807 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2684 10
Size: 54 1425 343 852 3324
Fragments arranged by size:
3324 1425 852 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3083 3956 4315 4474 518
Size: 648 1917 873 359 159 2042
Fragments arranged by size:
2042 1917 873 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2141 2398 2998 3414 4091 5239 5484 1606
Size: 535 257 600 416 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 600 535 416 257 245
NaeI GCC'GGC
Cuts at: 2111 2111
Size: 5998
NarI GG'CG_CC
Cuts at: 2240 2240
Size: 5998
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5446
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NgoMIV G'CCGG_C
Cuts at: 2109 2109
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 3356 4901 1132
Size: 97 2127 1545 2229
Fragments arranged by size:
2229 2127 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5998
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5096
PfoI T'CCnGG_A
Cuts at: 2496 2496
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2560 4067 4099 5580 5612 2528
Size: 32 1507 32 1481 32 2914
Fragments arranged by size:
2914 1507 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2224 2377 2656 2668 2101
Size: 123 153 279 12 5431
Fragments arranged by size:
5431 279 153 123 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PspOMI G'GGCC_C
Cuts at: 2869 2869
Size: 5998
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 5966
PstI C_TGCA'G
Cuts at: 830 2135 2277 3138 3220 3276 3350 830
Size: 1305 142 861 82 56 74 3478
Fragments arranged by size:
3478 1305 861 142 82 74 56
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2141 2398 2998 2141
Size: 257 600 5141
Fragments arranged by size:
5141 600 257
SacI G_AGCT'C
Cuts at: 3204 3204
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SbfI CC_TGCA'GG
Cuts at: 3138 3350 3138
Size: 212 5786
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SexAI A'CCwGG_T
Cuts at: 2739 2739
Size: 5998
SfcI C'TryA_G
Cuts at: 826 1045 2044 2131 2273 3134 3216 3272 3346
Size: 219 999 87 142 861 82 56 74
Cuts at: 3346 4481 5162 5353 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 999 861 681 219 191 142
87 82 74 56
SfiI GGCCn_nnn'nGGCC
Cuts at: 2831 2831
Size: 5998
SfoI GGC'GCC
Cuts at: 2241 2241
Size: 5998
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 2654 3197 3325 1872
Size: 782 543 128 4545
Fragments arranged by size:
4545 782 543 128
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2588 4102 5003 5265 820
Size: 197 926 645 1514 901 262 1553
Fragments arranged by size:
1553 1514 926 901 645 262 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 2040 1517
Size: 523 5475
StyI C'CwwG_G
Cuts at: 513 1065 2202 2415 3437 513
Size: 552 1137 213 1022 3074
Fragments arranged by size:
3074 1137 1022 552 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4025 4042 4195 4380 4825 325
Size: 1255 2445 17 153 185 445 1498
Fragments arranged by size:
2445 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2388 4244 5956
Size: 274 80 33 51 1443 411 1856 1712
Cuts at: 5956 96
Size: 138
Fragments arranged by size:
1856 1712 1443 411 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5958 5990 5958
Size: 32 5966
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2206 2590 3151 2206
Size: 384 561 5053
Fragments arranged by size:
5053 561 384
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 2652 3195 3323 1870
Size: 782 543 128 4545
Fragments arranged by size:
4545 782 543 128
XmnI GAAnn'nnTTC
Cuts at: 4127 4127
Size: 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI
PacI PmlI PspXI RsrII SacII SanDI SapI SrfI
SwaI