Restriction map for
pFN21AB6605
(Circular) (Six-base) MAPSORT of: pFN21AB6605.seq Check: 9480 from: 1 to: 5884
pFN21AB6605 5884 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 2339 2654 2757 2985 3947 5331 5599
Size: 485 222 315 103 228 962 1384 268
Cuts at: 5599 1632
Size: 1917
Fragments arranged by size:
1917 1384 962 485 315 268 228 222
103
AflII C'TTAA_G
Cuts at: 820 1017 2306 820
Size: 197 1289 4398
Fragments arranged by size:
4398 1289 197
AflIII A'CryG_T
Cuts at: 1203 4783 5760 1203
Size: 3580 977 1327
Fragments arranged by size:
3580 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2228 1184
Size: 1044 4840
AlwNI CAG_nnn'CTG
Cuts at: 2768 3627 5199 2768
Size: 859 1572 3453
Fragments arranged by size:
3453 1572 859
ApaI G_GGCC'C
Cuts at: 2150 2150
Size: 5884
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 2453 2579 2873 3193 3419 3571 5680 1493
Size: 960 126 294 320 226 152 2109 1697
Fragments arranged by size:
2109 1697 960 320 294 226 152 126
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 3209 1870
Size: 73 1266 4545
Fragments arranged by size:
4545 1266 73
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3099 3205 4660 618
Size: 325 217 627 1312 106 1455 1842
Fragments arranged by size:
1842 1455 1312 627 325 217 106
BanII G_rGCy'C
Cuts at: 1391 2150 3203 1391
Size: 759 1053 4072
Fragments arranged by size:
4072 1053 759
BbeI G_GCGC'C
Cuts at: 3103 3103
Size: 5884
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2344 2389 928
Size: 971 445 45 4423
Fragments arranged by size:
4423 971 445 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4075 4109 1903
Size: 34 50 34 2054 34 3678
Fragments arranged by size:
3678 2054 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 2344 1548
Size: 796 5088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4495 136
Size: 107 122 71 631 605 2823 1525
Fragments arranged by size:
2823 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2606 5879 2606
Size: 3273 2611
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2150 3945 5101 1790
Size: 226 134 1795 1156 2573
Fragments arranged by size:
2573 1795 1156 226 134
BmgBI CAC'GTC
Cuts at: 1318 2534 3059 3086 1318
Size: 1216 525 27 4116
Fragments arranged by size:
4116 1216 525 27
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2683 3615 4573
Size: 416 53 83 399 24 1266 932 958
Cuts at: 4573 442
Size: 1753
Fragments arranged by size:
1753 1266 958 932 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2184 2184
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3044 4544 1239
Size: 1805 1500 2579
Fragments arranged by size:
2579 1805 1500
Bpu10I CC'TnA_GC
Cuts at: 2169 2169
Size: 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4009 4874 5172 4009
Size: 865 298 4721
Fragments arranged by size:
4721 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3033 4547 882
Size: 617 1534 1514 2219
Fragments arranged by size:
2219 1534 1514 617
BsaAI yAC'GTr
Cuts at: 493 1204 3675 493
Size: 711 2471 2702
Fragments arranged by size:
2702 2471 711
BsaBI GATnn'nnATC
Cuts at: 5878 5878
Size: 5884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3100 3691 4073 4708
Size: 53 83 186 936 1567 591 382 635
Cuts at: 4708 275
Size: 1451
Fragments arranged by size:
1567 1451 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2991 4317 4715 4989 5136 5547 2991
Size: 1326 398 274 147 411 3328
Fragments arranged by size:
3328 1326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5659
Fragments arranged by size:
5659 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2562 2819 1186
Size: 1111 265 257 4251
Fragments arranged by size:
4251 1111 265 257
BseYI C'CCAG_C
Cuts at: 2255 3172 5087 5583 2255
Size: 917 1915 496 2556
Fragments arranged by size:
2556 1915 917 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5311
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3334 4095 5123 1540
Size: 275 186 1333 761 1028 2301
Fragments arranged by size:
2301 1333 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3945 4030 5101 3945
Size: 85 1071 4728
Fragments arranged by size:
4728 1071 85
BsmI GAATG_Cn'
Cuts at: 3167 3395 3488 3167
Size: 228 93 5563
Fragments arranged by size:
5563 228 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2279 3021 2279
Size: 742 5142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2150 3203 3945 4030 5101 1391
Size: 399 226 134 1053 742 85 1071 2174
Fragments arranged by size:
2174 1071 1053 742 399 226 134 85
BspEI T'CCGG_A
Cuts at: 2991 5547 2991
Size: 2556 3328
BspHI T'CATG_A
Cuts at: 3773 5503 3773
Size: 1730 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3239 844
Size: 2395 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4379 4553 65
Size: 1183 3131 174 1396
Fragments arranged by size:
3131 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2126 4528 4715 1700
Size: 110 316 2402 187 2869
Fragments arranged by size:
2869 2402 316 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 2403 2565 2639 3944 4956 2403
Size: 162 74 1305 1012 3331
Fragments arranged by size:
3331 1305 1012 162 74
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5884
BstBI TT'CG_AA
Cuts at: 2418 4688 2418
Size: 2270 3614
BstEII G'GTnAC_C
Cuts at: 1184 2041 1184
Size: 857 5027
BstYI r'GATC_y
Cuts at: 1972 2606 2663 3214 3581 3966 3983 5435 5521
Size: 634 57 551 367 385 17 1452 86
Cuts at: 5521 5533 5879 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 634 551 385 367 346 86
57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2175 2298 2527 3109 513
Size: 552 1110 123 229 582 3288
Fragments arranged by size:
3288 1110 582 552 229 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5145
Fragments arranged by size:
5145 683 56
BtsI GCAGTG_nn'
Cuts at: 2918 3400 4194 4214 2918
Size: 482 794 20 4588
Fragments arranged by size:
4588 794 482 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DraIII CAC_nnn'GTG
Cuts at: 2405 2405
Size: 5884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3127 4891 809
Size: 507 1811 1764 1802
Fragments arranged by size:
1811 1802 1764 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3046 3331 4220
Size: 54 1425 50 275 18 1216 285 889
Cuts at: 4220 5849 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1216 889 285 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2506 2787 2946 3814 1353
Size: 1153 281 159 868 3423
Fragments arranged by size:
3423 1153 868 281 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4471 4845 4991 387
Size: 1064 138 2882 374 146 1280
Fragments arranged by size:
2882 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2339 2654 2757 2985 3044 3947
Size: 393 485 222 315 103 228 59 903
Cuts at: 3947 4544 5331 5599 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 903 787 597 485 393 315 268
228 222 103 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2490 2490
Size: 5884
EcoO109I rG'GnC_Cy
Cuts at: 2146 2147 2146
Size: 1 5883
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FspI TGC'GCA
Cuts at: 2216 4390 2216
Size: 2174 3710
HaeII r_GCGC'y
Cuts at: 1678 2306 3103 5031 1678
Size: 628 797 1928 2531
Fragments arranged by size:
2531 1928 797 628
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2999 3031 4525
Size: 32 135 32 488 32 907 32 1494
Cuts at: 4525 4557 4599 4631 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1494 907 488 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 3228 3474 669
Size: 988 281 334 956 246 3079
Fragments arranged by size:
3079 988 956 334 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KasI G'GCGC_C
Cuts at: 3099 3099
Size: 5884
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2217 2311 4998 5182 1305
Size: 912 94 2687 184 2007
Fragments arranged by size:
2687 2007 912 184 94
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4062
Fragments arranged by size:
4062 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2625 3842 4201 4360 518
Size: 648 1459 1217 359 159 2042
Fragments arranged by size:
2042 1459 1217 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2177 2212 2248 3022 3300 3977 5125 5370
Size: 571 35 36 774 278 677 1148 245
Cuts at: 5370 1606
Size: 2120
Fragments arranged by size:
2120 1148 774 677 571 278 245 36
35
NaeI GCC'GGC
Cuts at: 2128 2128
Size: 5884
NarI GG'CG_CC
Cuts at: 3100 3100
Size: 5884
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5332
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NgoMIV G'CCGG_C
Cuts at: 2126 2126
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 3242 4787 1132
Size: 97 2013 1545 2229
Fragments arranged by size:
2229 2013 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2150 1348
Size: 802 5082
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3059 3110 1108
Size: 1951 51 3882
Fragments arranged by size:
3882 1951 51
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PspOMI G'GGCC_C
Cuts at: 2146 2146
Size: 5884
PstI C_TGCA'G
Cuts at: 830 3236 830
Size: 2406 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
PvuII CAG'CTG
Cuts at: 2212 3022 2212
Size: 810 5074
SacII CC_GC'GG
Cuts at: 2178 2178
Size: 5884
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SfcI C'TryA_G
Cuts at: 826 1045 3232 4367 5048 5239 826
Size: 219 2187 1135 681 191 1471
Fragments arranged by size:
2187 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3101 3101
Size: 5884
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 3211 1872
Size: 1339 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2306 3988 4889 5151 820
Size: 197 926 363 1682 901 262 1553
Fragments arranged by size:
1682 1553 926 901 363 262 197
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5884
StyI C'CwwG_G
Cuts at: 513 1065 3043 3323 513
Size: 552 1978 280 3074
Fragments arranged by size:
3074 1978 552 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2485 2496 3137 3911 3928 4081 4266
Size: 1255 905 11 641 774 17 153 185
Cuts at: 4266 4711 325
Size: 445 1498
Fragments arranged by size:
1498 1255 905 774 641 445 185 153
17 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4130 5842 96
Size: 274 80 33 51 1443 2153 1712 138
Fragments arranged by size:
2153 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5844 5876 5844
Size: 32 5852
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 2263
Size: 5884
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 3209 1870
Size: 1339 4545
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI
SwaI