Restriction map for
pFN21AB6596
(Circular) (Six-base) MAPSORT of: pFN21AB6596.seq Check: 9577 from: 1 to: 5926
pFN21AB6596 5926 bp
With 182 enzymes: SgfI *
March 28, 2012 12:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2862 3269 1937
Size: 925 407 4594
Fragments arranged by size:
4594 925 407
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2861 3184 3989 5373 5641 1632
Size: 1229 323 805 1384 268 1917
Fragments arranged by size:
1917 1384 1229 805 323 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 4825 5802 1203
Size: 3622 977 1327
Fragments arranged by size:
3622 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 2447 2598 2657 3086 1166
Size: 1281 151 59 429 4006
Fragments arranged by size:
4006 1281 429 151 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2742 1184
Size: 1558 4368
AlwNI CAG_nnn'CTG
Cuts at: 3669 5241 3669
Size: 1572 4354
ApaI G_GGCC'C
Cuts at: 3097 3097
Size: 5926
ApaLI G'TGCA_C
Cuts at: 2767 3983 5139 2767
Size: 1216 1156 3554
Fragments arranged by size:
3554 1216 1156
ApoI r'AATT_y
Cuts at: 1493 3235 3461 3613 5722 1493
Size: 1742 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2690 3251 1870
Size: 73 747 561 4545
Fragments arranged by size:
4545 747 561 73
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3247 4702 618
Size: 325 217 627 1460 1455 1842
Fragments arranged by size:
1842 1460 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2894 3097 3245 1391
Size: 1503 203 148 4072
Fragments arranged by size:
4072 1503 203 148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2344 928
Size: 971 445 4510
Fragments arranged by size:
4510 971 445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4117 4151 1903
Size: 34 2180 34 3678
Fragments arranged by size:
3678 2180 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BfrBI ATG'CAT
Cuts at: 2648 2648
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2246 4537 136
Size: 107 122 71 631 605 574 2291 1525
Fragments arranged by size:
2291 1525 631 605 574 122 107 71
BglII A'GATC_T
Cuts at: 2830 5921 2830
Size: 3091 2835
BlpI GC'TnA_GC
Cuts at: 3343 3343
Size: 5926
Bme1580I G_kGCm'C
Cuts at: 1790 2771 3097 3987 5143 1790
Size: 981 326 890 1156 2573
Fragments arranged by size:
2573 1156 981 890 326
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3657 4615 442
Size: 416 53 83 399 24 2240 958 1753
Fragments arranged by size:
2240 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 3014 3086 4586 1239
Size: 1163 612 72 1500 2579
Fragments arranged by size:
2579 1500 1163 612 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2513 3062 4589 882
Size: 617 1014 549 1527 2219
Fragments arranged by size:
2219 1527 1014 617 549
BsaAI yAC'GTr
Cuts at: 493 1204 3717 493
Size: 711 2513 2702
Fragments arranged by size:
2702 2513 711
BsaBI GATnn'nnATC
Cuts at: 2168 5920 2168
Size: 3752 2174
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3733 4115 4750 275
Size: 53 83 186 936 2200 382 635 1451
Fragments arranged by size:
2200 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2964 4359 4757 5031 5178 5589 2964
Size: 1395 398 274 147 411 3301
Fragments arranged by size:
3301 1395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2400 2430 3137 3167 1178
Size: 30 165 30 997 30 707 30 3937
Fragments arranged by size:
3937 997 707 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2689 3116 3119 3199 1186
Size: 1503 427 3 80 3913
Fragments arranged by size:
3913 1503 427 80 3
BseYI C'CCAG_C
Cuts at: 2528 5129 5625 2528
Size: 2601 496 2829
Fragments arranged by size:
2829 2601 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2771 2894 3222 3987 4072 5143 2771
Size: 123 328 765 85 1071 3554
Fragments arranged by size:
3554 1071 765 328 123 85
BsmI GAATG_Cn'
Cuts at: 2252 3437 3530 2252
Size: 1185 93 4648
Fragments arranged by size:
4648 1185 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2771 2894 3097 3222 3245 3987 4072
Size: 399 981 123 203 125 23 742 85
Cuts at: 4072 5143 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 981 742 399 203 125 123
85 23
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2848 3281 844
Size: 2004 433 3489
Fragments arranged by size:
3489 2004 433
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2987 4570 4757 1700
Size: 110 1177 1583 187 2869
Fragments arranged by size:
2869 1583 1177 187 110
BsrGI T'GTAC_A
Cuts at: 96 2901 3048 96
Size: 2805 147 2974
Fragments arranged by size:
2974 2805 147
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 2714 3098 3986 4998 2714
Size: 384 888 1012 3642
Fragments arranged by size:
3642 1012 888 384
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5926
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 2134 1184
Size: 950 4976
BstXI CCAn_nnnn'nTGG
Cuts at: 2449 3088 2449
Size: 639 5287
BstYI r'GATC_y
Cuts at: 1972 2685 2830 3256 3623 4008 4025 5477 5563
Size: 713 145 426 367 385 17 1452 86
Cuts at: 5563 5575 5921 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 713 426 385 367 346 145
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2244 513
Size: 552 939 240 4195
Fragments arranged by size:
4195 939 552 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2326 487
Size: 683 56 1100 4087
Fragments arranged by size:
4087 1100 683 56
BtsI GCAGTG_nn'
Cuts at: 3049 3442 4236 4256 3049
Size: 393 794 20 4719
Fragments arranged by size:
4719 794 393 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DraIII CAC_nnn'GTG
Cuts at: 3173 3173
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2400 2555 2868
Size: 54 1425 50 275 18 570 155 313
Cuts at: 2868 2883 3373 4262 5891 8
Size: 15 490 889 1629 43
Fragments arranged by size:
1629 1425 889 570 490 313 275 155
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3169 3856 1353
Size: 1816 687 3423
Fragments arranged by size:
3423 1816 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2892 2892
Size: 5926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2402 2861 3014 3086 3184 3989 4586
Size: 393 770 459 153 72 98 805 597
Cuts at: 4586 5373 5641 1239
Size: 787 268 1524
Fragments arranged by size:
1524 805 787 770 597 459 393 268
153 98 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2389 2389
Size: 5926
EcoO109I rG'GnC_Cy
Cuts at: 2470 3093 2470
Size: 623 5303
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 5926
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 2988 5073 1678
Size: 1310 2085 2531
Fragments arranged by size:
2531 2085 1310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3137 3169 4567 4599 4641
Size: 32 135 32 1565 32 1398 32 42
Cuts at: 4641 4673 1373
Size: 32 2626
Fragments arranged by size:
2626 1565 1398 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3180 5040 5224 1305
Size: 1875 1860 184 2007
Fragments arranged by size:
2007 1875 1860 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2402 2557 2870 2885 10
Size: 54 1425 343 570 155 313 15 3051
Fragments arranged by size:
3051 1425 570 343 313 155 54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 2447 2598 2600 2657 3086 3884
Size: 648 966 315 151 2 57 429 798
Cuts at: 3884 4243 4402 518
Size: 359 159 2042
Fragments arranged by size:
2042 966 798 648 429 359 315 159
151 57 2
MspA1I CmG'CkG
Cuts at: 1606 2114 2348 3342 4019 5167 5412 1606
Size: 508 234 994 677 1148 245 2120
Fragments arranged by size:
2120 1148 994 677 508 245 234
NaeI GCC'GGC
Cuts at: 2989 2989
Size: 5926
NcoI C'CATG_G
Cuts at: 513 1065 2004 2244 513
Size: 552 939 240 4195
Fragments arranged by size:
4195 939 552 240
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NgoMIV G'CCGG_C
Cuts at: 2987 2987
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NsiI A_TGCA'T
Cuts at: 2650 2650
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 2960 3284 4829 1132
Size: 97 1731 324 1545 2229
Fragments arranged by size:
2229 1731 1545 324 97
PasI CC'CwG_GG
Cuts at: 1348 2497 3037 1348
Size: 1149 540 4237
Fragments arranged by size:
4237 1149 540
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2784 2877 1108
Size: 1676 93 4157
Fragments arranged by size:
4157 1676 93
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2470 2470
Size: 5926
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PspOMI G'GGCC_C
Cuts at: 3093 3093
Size: 5926
PstI C_TGCA'G
Cuts at: 830 3278 830
Size: 2448 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2114 2114
Size: 5926
SacI G_AGCT'C
Cuts at: 2894 2894
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SapI GCTCTTCn'nnn_
Cuts at: 3169 3169
Size: 5926
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 3274 4409 5090 5281 826
Size: 219 2229 1135 681 191 1471
Fragments arranged by size:
2229 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 2987 1700
Size: 1287 4639
SmaI CCC'GGG
Cuts at: 1872 2692 3253 1872
Size: 820 561 4545
Fragments arranged by size:
4545 820 561
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4030 4931 5193 820
Size: 197 926 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 2960 3284 1132
Size: 1828 324 3774
Fragments arranged by size:
3774 1828 324
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 2240 1517
Size: 723 5203
StyI C'CwwG_G
Cuts at: 513 1065 2004 2244 3123 3365 513
Size: 552 939 240 879 242 3074
Fragments arranged by size:
3074 939 879 552 242 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3953 3970 4123 4308 4753 325
Size: 1255 2373 17 153 185 445 1498
Fragments arranged by size:
2373 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2901 3048 4172
Size: 274 80 33 51 1443 924 147 1124
Cuts at: 4172 5884 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1124 924 274 147 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5886 5918 5886
Size: 32 5894
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2377 2737 2878 3082 2377
Size: 360 141 204 5221
Fragments arranged by size:
5221 360 204 141
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 2690 3251 1870
Size: 820 561 4545
Fragments arranged by size:
4545 820 561
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NarI NruI PacI PfoI PmlI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI