Restriction map for
pFN21AB6595
(Circular) (Six-base) MAPSORT of: pFN21AB6595.seq Check: 3911 from: 1 to: 5845
pFN21AB6595 5845 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3188 1937
Size: 1251 4594
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2064 3908 5292 5560 1632
Size: 432 1844 1384 268 1917
Fragments arranged by size:
1917 1844 1384 432 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 4744 5721 1203
Size: 3541 977 1327
Fragments arranged by size:
3541 1327 977
AgeI A'CCGG_T
Cuts at: 3080 4676 3080
Size: 1596 4249
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5845
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2343 2375 2343
Size: 32 5813
AlwNI CAG_nnn'CTG
Cuts at: 3588 5160 3588
Size: 1572 4273
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 2406 2964 3128 3154 3380 3532 5641 1493
Size: 913 558 164 26 226 152 2109 1697
Fragments arranged by size:
2109 1697 913 558 226 164 152 26
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 2745 3170 1870
Size: 73 802 425 4545
Fragments arranged by size:
4545 802 425 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2128 2161 2128
Size: 33 5812
BamHI G'GATC_C
Cuts at: 2128 3175 3542 2128
Size: 1047 367 4431
Fragments arranged by size:
4431 1047 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3166 4621 618
Size: 325 217 627 1379 1455 1842
Fragments arranged by size:
1842 1455 1379 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3164 1391
Size: 1773 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2255 3012 928
Size: 971 356 757 3761
Fragments arranged by size:
3761 971 757 356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2898 2932 4036 4070 1903
Size: 34 961 34 1104 34 3678
Fragments arranged by size:
3678 1104 961 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2129 3737 4947 2129
Size: 1608 1210 3027
Fragments arranged by size:
3027 1608 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BfrBI ATG'CAT
Cuts at: 2546 2546
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4456 136
Size: 107 122 71 631 605 2784 1525
Fragments arranged by size:
2784 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5840 5840
Size: 5845
BlpI GC'TnA_GC
Cuts at: 3262 3262
Size: 5845
Bme1580I G_kGCm'C
Cuts at: 1790 3906 5062 1790
Size: 2116 1156 2573
Fragments arranged by size:
2573 2116 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3576 4534 442
Size: 416 53 83 399 24 2159 958 1753
Fragments arranged by size:
2159 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4505 1239
Size: 3266 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3970 4835 5133 3970
Size: 865 298 4682
Fragments arranged by size:
4682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4508 882
Size: 617 3009 2219
Fragments arranged by size:
3009 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3636 493
Size: 711 2432 2702
Fragments arranged by size:
2702 2432 711
BsaBI GATnn'nnATC
Cuts at: 5839 5839
Size: 5845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3652 4034 4669 275
Size: 53 83 186 936 2119 382 635 1451
Fragments arranged by size:
2119 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3080 4278 4676 4950 5097 5508 3080
Size: 1198 398 274 147 411 3417
Fragments arranged by size:
3417 1198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5620
Fragments arranged by size:
5620 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2097 1186
Size: 911 4934
BseYI C'CCAG_C
Cuts at: 2994 5048 5544 2994
Size: 2054 496 3295
Fragments arranged by size:
3295 2054 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3295 4056 5084 1540
Size: 275 186 1294 761 1028 2301
Fragments arranged by size:
2301 1294 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3906 3991 5062 3906
Size: 85 1071 4689
Fragments arranged by size:
4689 1071 85
BsmI GAATG_Cn'
Cuts at: 2201 3356 3449 2201
Size: 1155 93 4597
Fragments arranged by size:
4597 1155 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3164 3906 3991 5062 1391
Size: 399 1374 742 85 1071 2174
Fragments arranged by size:
2174 1374 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 3734 5464 3734
Size: 1730 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3200 844
Size: 2356 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3732 1394
Size: 429 1909 3507
Fragments arranged by size:
3507 1909 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2165 2345 4340 4514 65
Size: 1183 917 180 1995 174 1396
Fragments arranged by size:
1995 1396 1183 917 180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3080 4489 4676 1700
Size: 110 1270 1409 187 2869
Fragments arranged by size:
2869 1409 1270 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5845
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5845
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 2128 2675 3175 3542 3927 3944 5396 5482
Size: 156 547 500 367 385 17 1452 86
Cuts at: 5482 5494 5840 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 547 500 385 367 346 156
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2109 2109
Size: 5845
BtgI C'CryG_G
Cuts at: 513 1065 2004 2333 513
Size: 552 939 329 4025
Fragments arranged by size:
4025 939 552 329
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5106
Fragments arranged by size:
5106 683 56
BtsI GCAGTG_nn'
Cuts at: 3361 4155 4175 3361
Size: 794 20 5031
Fragments arranged by size:
5031 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3292 4181 5810
Size: 54 1425 50 275 18 1462 889 1629
Cuts at: 5810 8
Size: 43
Fragments arranged by size:
1629 1462 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3292 1537
Size: 275 1480 4090
Fragments arranged by size:
4090 1480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3775 1353
Size: 2422 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 3908 4505 5292 5560 1239
Size: 393 432 1844 597 787 268 1524
Fragments arranged by size:
1844 1524 787 597 432 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2484 2484
Size: 5845
EcoO109I rG'GnC_Cy
Cuts at: 2792 2792
Size: 5845
EcoRI G'AATT_C
Cuts at: 2406 3154 3532 2406
Size: 748 378 4719
Fragments arranged by size:
4719 748 378
EcoRV GAT'ATC
Cuts at: 2394 2394
Size: 5845
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 4992 1678
Size: 3314 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 4486 4518 4560
Size: 32 135 32 541 32 2341 32 42
Cuts at: 4560 4592 1373
Size: 32 2626
Fragments arranged by size:
2626 2341 541 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2101 3189 3435 669
Size: 988 281 163 1088 246 3079
Fragments arranged by size:
3079 1088 988 281 246 163
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 2101 3435 2101
Size: 1334 4511
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 2449 3444 2449
Size: 995 4850
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4959 5143 1305
Size: 3654 184 2007
Fragments arranged by size:
3654 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4023
Fragments arranged by size:
4023 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2338 2551 3803 4162 4321 518
Size: 648 1172 213 1252 359 159 2042
Fragments arranged by size:
2042 1252 1172 648 359 213 159
MspA1I CmG'CkG
Cuts at: 1606 3261 3938 5086 5331 1606
Size: 1655 677 1148 245 2120
Fragments arranged by size:
2120 1655 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2333 513
Size: 552 939 329 4025
Fragments arranged by size:
4025 939 552 329
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NsiI A_TGCA'T
Cuts at: 2548 2548
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 3203 4748 1132
Size: 97 1974 1545 2229
Fragments arranged by size:
2229 1974 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5845
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5845
PfoI T'CCnGG_A
Cuts at: 2131 2131
Size: 5845
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2792 2792
Size: 5845
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PstI C_TGCA'G
Cuts at: 830 3197 830
Size: 2367 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SfcI C'TryA_G
Cuts at: 826 1045 2862 3193 4328 5009 5200 826
Size: 219 1817 331 1135 681 191 1471
Fragments arranged by size:
1817 1471 1135 681 331 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3949 4850 5112 820
Size: 197 926 2006 901 262 1553
Fragments arranged by size:
2006 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 2459 2944 3063 3769 5
Size: 47 2407 485 119 706 2081
Fragments arranged by size:
2407 2081 706 485 119 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5845
StyI C'CwwG_G
Cuts at: 513 1065 2004 2333 2850 3284 513
Size: 552 939 329 517 434 3074
Fragments arranged by size:
3074 939 552 517 434 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3872 3889 4042 4227 4672 325
Size: 1255 2292 17 153 185 445 1498
Fragments arranged by size:
2292 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2069 2491 4091
Size: 274 80 33 51 1443 92 422 1600
Cuts at: 4091 5803 96
Size: 1712 138
Fragments arranged by size:
1712 1600 1443 422 274 138 92 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5805 5837 5805
Size: 32 5813
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI