Restriction map for
pFN21AB6592
(Circular) (Six-base) MAPSORT of: pFN21AB6592.seq Check: 9641 from: 1 to: 5923
pFN21AB6592 5923 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2510 3986 5370 5638 1632
Size: 878 1476 1384 268 1917
Fragments arranged by size:
1917 1476 1384 878 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 2581 4822 5799 1203
Size: 1378 2241 977 1327
Fragments arranged by size:
2241 1378 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 2661 1166
Size: 1495 4428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2796 1184
Size: 1612 4311
AlwNI CAG_nnn'CTG
Cuts at: 2092 2200 3666 5238 2092
Size: 108 1466 1572 2777
Fragments arranged by size:
2777 1572 1466 108
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 2044 2391 2603 2843 3232 3458 3610 5719
Size: 551 347 212 240 389 226 152 2109
Cuts at: 5719 1493
Size: 1697
Fragments arranged by size:
2109 1697 551 389 347 240 226 212
152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 3248 1870
Size: 73 1305 4545
Fragments arranged by size:
4545 1305 73
BamHI G'GATC_C
Cuts at: 2790 3253 3620 2790
Size: 463 367 5093
Fragments arranged by size:
5093 463 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2766 3244 4699 618
Size: 325 217 627 979 478 1455 1842
Fragments arranged by size:
1842 1455 979 627 478 325 217
BanII G_rGCy'C
Cuts at: 1391 2637 2983 3242 1391
Size: 1246 346 259 4072
Fragments arranged by size:
4072 1246 346 259
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2714 2748 4114 4148 1903
Size: 34 777 34 1366 34 3678
Fragments arranged by size:
3678 1366 777 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3215 4534
Size: 107 122 71 631 605 337 1206 1319
Cuts at: 4534 136
Size: 1525
Fragments arranged by size:
1525 1319 1206 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2065 5918 2065
Size: 3853 2070
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 3984 5140 1790
Size: 2194 1156 2573
Fragments arranged by size:
2573 2194 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3654 4612 442
Size: 416 53 83 399 24 2237 958 1753
Fragments arranged by size:
2237 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2744 1056
Size: 1688 4235
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2073 2073
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 2459 2492 4583 1239
Size: 897 323 33 2091 2579
Fragments arranged by size:
2579 2091 897 323 33
Bpu10I CC'TnA_GC
Cuts at: 3003 3219 3003
Size: 216 5707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2582 3714 493
Size: 711 1378 1132 2702
Fragments arranged by size:
2702 1378 1132 711
BsaBI GATnn'nnATC
Cuts at: 2481 5917 2481
Size: 3436 2487
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3730 4112 4747 275
Size: 53 83 186 936 2197 382 635 1451
Fragments arranged by size:
2197 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5698
Fragments arranged by size:
5698 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 1186
Size: 1132 4791
BseYI C'CCAG_C
Cuts at: 2034 5126 5622 2034
Size: 3092 496 2335
Fragments arranged by size:
3092 2335 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2601 1163
Size: 573 865 4485
Fragments arranged by size:
4485 865 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2637 2983 3984 4069 5140 2637
Size: 346 1001 85 1071 3420
Fragments arranged by size:
3420 1071 1001 346 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2637 2983 3242 3984 4069 5140 1391
Size: 399 847 346 259 742 85 1071 2174
Fragments arranged by size:
2174 1071 847 742 399 346 259 85
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 2040 3812 5542 2040
Size: 1772 1730 2421
Fragments arranged by size:
2421 1772 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2299 3278 844
Size: 1455 979 3489
Fragments arranged by size:
3489 1455 979
BsrBI CCG'CTC
Cuts at: 1394 1823 2052 3810 1394
Size: 429 229 1758 3507
Fragments arranged by size:
3507 1758 429 229
BsrDI GCAATG_nn'
Cuts at: 65 1248 2163 4418 4592 65
Size: 1183 915 2255 174 1396
Fragments arranged by size:
2255 1396 1183 915 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4567 4754 1700
Size: 110 2757 187 2869
Fragments arranged by size:
2869 2757 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 2180 3093 3983 4995 2180
Size: 913 890 1012 3108
Fragments arranged by size:
3108 1012 913 890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 2967 4727 2967
Size: 1760 4163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstXI CCAn_nnnn'nTGG
Cuts at: 2902 3071 2902
Size: 169 5754
BstYI r'GATC_y
Cuts at: 1972 2065 2088 2277 2790 2991 3123 3253 3620
Size: 93 23 189 513 201 132 130 367
Cuts at: 3620 4005 4022 5474 5560 5572 5918 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 513 385 367 346 201 189
132 130 93 86 23 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3201 513
Size: 552 939 1197 3235
Fragments arranged by size:
3235 1197 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 3439 4233 4253 3439
Size: 794 20 5109
Fragments arranged by size:
5109 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3370 4259 5888
Size: 54 1425 50 275 18 1540 889 1629
Cuts at: 5888 8
Size: 43
Fragments arranged by size:
1629 1540 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3853 1353
Size: 2500 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3023 4510 4884 5030 387
Size: 1064 138 1434 1487 374 146 1280
Fragments arranged by size:
1487 1434 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2635 2981 2635
Size: 346 5577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2459 2492 2510 3986 4583 5370
Size: 393 504 323 33 18 1476 597 787
Cuts at: 5370 5638 1239
Size: 268 1524
Fragments arranged by size:
1524 1476 787 597 504 393 323 268
33 18
EcoRI G'AATT_C
Cuts at: 2044 2391 3232 3610 2044
Size: 347 841 378 4357
Fragments arranged by size:
4357 841 378 347
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 5070 1678
Size: 3392 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3007 3039 4564 4596 4638
Size: 32 135 32 1435 32 1525 32 42
Cuts at: 4638 4670 1373
Size: 32 2626
Fragments arranged by size:
2626 1525 1435 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2133 2142 748
Size: 1385 9 4529
Fragments arranged by size:
4529 1385 9
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2661 2858 3184 3881 4240 4399 518
Size: 648 1495 197 326 697 359 159 2042
Fragments arranged by size:
2042 1495 697 648 359 326 197 159
MspA1I CmG'CkG
Cuts at: 1606 2885 3339 4016 5164 5409 1606
Size: 1279 454 677 1148 245 2120
Fragments arranged by size:
2120 1279 1148 677 454 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3201 513
Size: 552 939 1197 3235
Fragments arranged by size:
3235 1197 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 2740 1052
Size: 1688 4235
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 2690 3281 4826 1132
Size: 97 1461 591 1545 2229
Fragments arranged by size:
2229 1545 1461 591 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2383 1108
Size: 1275 4648
PfoI T'CCnGG_A
Cuts at: 2091 2091
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PmlI CAC'GTG
Cuts at: 2582 2582
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2651 1613
Size: 1038 4885
PsiI TTA'TAA
Cuts at: 2433 2565 3493 2433
Size: 132 928 4863
Fragments arranged by size:
4863 928 132
PstI C_TGCA'G
Cuts at: 830 3001 3275 830
Size: 2171 274 3478
Fragments arranged by size:
3478 2171 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2885 2885
Size: 5923
SacI G_AGCT'C
Cuts at: 2637 2983 2637
Size: 346 5577
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 2880 2997 3271 4406 5087 5278 826
Size: 219 1835 117 274 1135 681 191 1471
Fragments arranged by size:
1835 1471 1135 681 274 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4027 4928 5190 820
Size: 197 926 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 2959 3847 5
Size: 47 2907 888 2081
Fragments arranged by size:
2907 2081 888 47
StuI AGG'CCT
Cuts at: 1517 2678 1517
Size: 1161 4762
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 3201 3362 513
Size: 552 939 264 933 161 3074
Fragments arranged by size:
3074 939 933 552 264 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3950 3967 4120 4305 4750 325
Size: 1255 2370 17 153 185 445 1498
Fragments arranged by size:
2370 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 4169 5881
Size: 274 80 33 51 1443 441 1751 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1751 1712 1443 441 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2206 2238 2750 2782 3123 3155 5883 5915 2206
Size: 32 512 32 341 32 2728 32 2214
Fragments arranged by size:
2728 2214 512 341 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 2809 3259 2809
Size: 450 5473
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI