Restriction map for
pFN21AB6591
(Circular) (Six-base) MAPSORT of: pFN21AB6591.seq Check: 4965 from: 1 to: 5845
pFN21AB6591 5845 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2619 3655 278
Size: 53 83 186 936 1083 1036 2468
Fragments arranged by size:
2468 1083 1036 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2199 2358 3188 1937
Size: 262 159 830 4594
Fragments arranged by size:
4594 830 262 159
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3068 3908 5292 5560 1632
Size: 1436 840 1384 268 1917
Fragments arranged by size:
1917 1436 1384 840 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 2427 4744 5721 1203
Size: 1224 2317 977 1327
Fragments arranged by size:
2317 1327 1224 977
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2570 1184
Size: 1386 4459
AlwNI CAG_nnn'CTG
Cuts at: 2088 3588 5160 2088
Size: 1500 1572 2773
Fragments arranged by size:
2773 1572 1500
ApaI G_GGCC'C
Cuts at: 2045 2045
Size: 5845
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 2368 3154 3380 3532 5641 1493
Size: 875 786 226 152 2109 1697
Fragments arranged by size:
2109 1697 875 786 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2790 3170 1870
Size: 73 113 734 380 4545
Fragments arranged by size:
4545 734 380 113 73
BamHI G'GATC_C
Cuts at: 3100 3175 3542 3100
Size: 75 367 5403
Fragments arranged by size:
5403 367 75
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2597 3166 4621 618
Size: 325 217 627 810 569 1455 1842
Fragments arranged by size:
1842 1455 810 627 569 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2112 2157 3164 1391
Size: 654 67 45 1007 4072
Fragments arranged by size:
4072 1007 654 67 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 2619 2686 2842 928
Size: 971 383 337 67 156 3931
Fragments arranged by size:
3931 971 383 337 156 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4036 4070 1903
Size: 34 2099 34 3678
Fragments arranged by size:
3678 2099 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 2253 1548
Size: 705 5140
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4456 136
Size: 107 122 71 631 605 2784 1525
Fragments arranged by size:
2784 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2022 2685 5840 2022
Size: 663 3155 2027
Fragments arranged by size:
3155 2027 663
BlpI GC'TnA_GC
Cuts at: 3262 3262
Size: 5845
Bme1580I G_kGCm'C
Cuts at: 1790 2045 3906 5062 1790
Size: 255 1861 1156 2573
Fragments arranged by size:
2573 1861 1156 255
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3576 4534 442
Size: 416 53 83 399 24 2159 958 1753
Fragments arranged by size:
2159 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2917 4505 1239
Size: 932 746 1588 2579
Fragments arranged by size:
2579 1588 932 746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2747 3970 4835 5133 2747
Size: 1223 865 298 3459
Fragments arranged by size:
3459 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4508 882
Size: 617 3009 2219
Fragments arranged by size:
3009 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3636 493
Size: 711 2432 2702
Fragments arranged by size:
2702 2432 711
BsaBI GATnn'nnATC
Cuts at: 2021 5839 2021
Size: 3818 2027
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2616 3652 4034 4669
Size: 53 83 186 936 1083 1036 382 635
Cuts at: 4669 275
Size: 1451
Fragments arranged by size:
1451 1083 1036 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4278 4676 4950 5097 5508 4278
Size: 398 274 147 411 4615
Fragments arranged by size:
4615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5620
Fragments arranged by size:
5620 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5845
BseYI C'CCAG_C
Cuts at: 2100 5048 5544 2100
Size: 2948 496 2401
Fragments arranged by size:
2948 2401 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3295 4056 5084 1540
Size: 275 186 1294 761 1028 2301
Fragments arranged by size:
2301 1294 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2636 2909 3074 3906 3991 5062 2636
Size: 273 165 832 85 1071 3419
Fragments arranged by size:
3419 1071 832 273 165 85
BsmI GAATG_Cn'
Cuts at: 3356 3449 3356
Size: 93 5752
BsmBI CGTCTCn'nnnn_
Cuts at: 2765 2765
Size: 5845
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2112 2157 2636 2909 3074 3164
Size: 399 255 67 45 479 273 165 90
Cuts at: 3164 3906 3991 5062 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 479 399 273 255 165
90 85 67 45
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 2679 3734 5464 2679
Size: 1055 1730 3060
Fragments arranged by size:
3060 1730 1055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 2817 3200 844
Size: 1213 760 383 3489
Fragments arranged by size:
3489 1213 760 383
BsrBI CCG'CTC
Cuts at: 1394 1823 3064 3732 1394
Size: 429 1241 668 3507
Fragments arranged by size:
3507 1241 668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2475 4340 4514 65
Size: 1183 1227 1865 174 1396
Fragments arranged by size:
1865 1396 1227 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 4489 4676 1700
Size: 110 319 2360 187 2869
Fragments arranged by size:
2869 2360 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5845
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5845
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 2022 2685 3100 3175 3542 3927 3944 5396
Size: 50 663 415 75 367 385 17 1452
Cuts at: 5396 5482 5494 5840 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 663 415 385 367 346 86
75 50 17 12
Bsu36I CC'TnA_GG
Cuts at: 2956 3113 2956
Size: 157 5688
BtgI C'CryG_G
Cuts at: 513 1065 2004 2029 513
Size: 552 939 25 4329
Fragments arranged by size:
4329 939 552 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2935 487
Size: 683 56 1709 3397
Fragments arranged by size:
3397 1709 683 56
BtsI GCAGTG_nn'
Cuts at: 3361 4155 4175 3361
Size: 794 20 5031
Fragments arranged by size:
5031 794 20
ClaI AT'CG_AT
Cuts at: 1554 3123 1554
Size: 1569 4276
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DraIII CAC_nnn'GTG
Cuts at: 2494 2494
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2621 4852 809
Size: 507 1305 2231 1802
Fragments arranged by size:
2231 1802 1305 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 3292 4181
Size: 54 1425 50 275 18 342 1120 889
Cuts at: 4181 5810 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1120 889 342 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3292 1537
Size: 275 1480 4090
Fragments arranged by size:
4090 1480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2681 3775 1353
Size: 1328 1094 3423
Fragments arranged by size:
3423 1328 1094
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2917 3068 3908 4505 5292 5560
Size: 393 539 746 151 840 597 787 268
Cuts at: 5560 1239
Size: 1524
Fragments arranged by size:
1524 840 787 746 597 539 393 268
151
EcoNI CCTnn'n_nnAGG
Cuts at: 2891 2891
Size: 5845
EcoO109I rG'GnC_Cy
Cuts at: 2042 2671 2042
Size: 629 5216
EcoRI G'AATT_C
Cuts at: 2368 3154 3532 2368
Size: 786 378 4681
Fragments arranged by size:
4681 786 378
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 2064 2088 2130 4992 1678
Size: 386 24 42 2862 2531
Fragments arranged by size:
2862 2531 386 42 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2645 2677 2743 2775 4486
Size: 32 135 32 1073 32 66 32 1711
Cuts at: 4486 4518 4560 4592 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1711 1073 135 66 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3189 3435 669
Size: 988 281 1251 246 3079
Fragments arranged by size:
3079 1251 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4959 5143 1305
Size: 3654 184 2007
Fragments arranged by size:
3654 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4023
Fragments arranged by size:
4023 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 2270 2480 2747 2888 3803 4162
Size: 648 1083 21 210 267 141 915 359
Cuts at: 4162 4321 518
Size: 159 2042
Fragments arranged by size:
2042 1083 915 648 359 267 210 159
141 21
MspA1I CmG'CkG
Cuts at: 1606 2031 2149 2924 3261 3938 5086 5331 1606
Size: 425 118 775 337 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 677 425 337 245 118
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 5845
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4354
Fragments arranged by size:
4354 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 2431 3203 4748 1132
Size: 97 1202 772 1545 2229
Fragments arranged by size:
2229 1545 1202 772 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5845
PciI A'CATG_T
Cuts at: 2427 4744 2427
Size: 2317 3528
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5845
PfoI T'CCnGG_A
Cuts at: 3027 3027
Size: 5845
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2671 2671
Size: 5845
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PspOMI G'GGCC_C
Cuts at: 2041 2041
Size: 5845
PstI C_TGCA'G
Cuts at: 830 3197 830
Size: 2367 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 2149 2924 2149
Size: 775 5070
SacII CC_GC'GG
Cuts at: 2032 2032
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SfcI C'TryA_G
Cuts at: 826 1045 3193 4328 5009 5200 826
Size: 219 2148 1135 681 191 1471
Fragments arranged by size:
2148 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 3949 4850 5112 820
Size: 197 926 819 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 819 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5845
StyI C'CwwG_G
Cuts at: 513 1065 2004 2590 3284 513
Size: 552 939 586 694 3074
Fragments arranged by size:
3074 939 694 586 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3872 3889 4042 4227 4672 325
Size: 1255 2292 17 153 185 445 1498
Fragments arranged by size:
2292 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4091 5803 96
Size: 274 80 33 51 1443 2114 1712 138
Fragments arranged by size:
2114 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2378 2410 5805 5837 2378
Size: 32 3395 32 2386
Fragments arranged by size:
3395 2386 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2461 2830 2461
Size: 369 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2617 3653 276
Size: 53 83 186 936 1083 1036 2468
Fragments arranged by size:
2468 1083 1036 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI