Restriction map for
pFN21AB6590
(Circular) (Six-base) MAPSORT of: pFN21AB6590.seq Check: 2724 from: 1 to: 5923
pFN21AB6590 5923 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2673 3733 278
Size: 53 83 186 936 1137 1060 2468
Fragments arranged by size:
2468 1137 1060 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2877 3266 1937
Size: 940 389 4594
Fragments arranged by size:
4594 940 389
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 3986 5370 5638 1632
Size: 530 1824 1384 268 1917
Fragments arranged by size:
1917 1824 1384 530 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 2882 4754 2882
Size: 1872 4051
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2427 2459 2427
Size: 32 5891
AlwNI CAG_nnn'CTG
Cuts at: 3666 5238 3666
Size: 1572 4351
ApaI G_GGCC'C
Cuts at: 2990 3145 2990
Size: 155 5768
ApaLI G'TGCA_C
Cuts at: 3088 3980 5136 3088
Size: 892 1156 3875
Fragments arranged by size:
3875 1156 892
ApoI r'AATT_y
Cuts at: 1493 2704 2767 3232 3458 3610 5719 1493
Size: 1211 63 465 226 152 2109 1697
Fragments arranged by size:
2109 1697 1211 465 226 152 63
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2177 3248 1870
Size: 73 234 1071 4545
Fragments arranged by size:
4545 1071 234 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 2338 3244 4699 618
Size: 325 217 627 495 56 906 1455 1842
Fragments arranged by size:
1842 1455 906 627 495 325 217 56
BanII G_rGCy'C
Cuts at: 1391 2060 2990 3145 3242 1391
Size: 669 930 155 97 4072
Fragments arranged by size:
4072 930 669 155 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2665 2862 928
Size: 971 766 197 3989
Fragments arranged by size:
3989 971 766 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3190 3224 4114 4148 1903
Size: 34 1253 34 890 34 3678
Fragments arranged by size:
3678 1253 890 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3149 4534 136
Size: 107 122 71 631 605 1477 1385 1525
Fragments arranged by size:
1525 1477 1385 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 2343 2990 3092 3145 3984 5140 1790
Size: 553 647 102 53 839 1156 2573
Fragments arranged by size:
2573 1156 839 647 553 102 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2251 3654 4612
Size: 416 53 83 399 24 834 1403 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1753 1403 958 834 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2395 4583 1239
Size: 1156 2188 2579
Fragments arranged by size:
2579 2188 1156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2096 4048 4913 5211 2096
Size: 1952 865 298 2808
Fragments arranged by size:
2808 1952 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2078 4586 882
Size: 617 579 2508 2219
Fragments arranged by size:
2508 2219 617 579
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 2276 5917 2276
Size: 3641 2282
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2670 3730 4112 4747
Size: 53 83 186 936 1137 1060 382 635
Cuts at: 4747 275
Size: 1451
Fragments arranged by size:
1451 1137 1060 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2882 4356 4754 5028 5175 5586 2882
Size: 1474 398 274 147 411 3219
Fragments arranged by size:
3219 1474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2207 2237 2427 2457 2630
Size: 30 165 30 804 30 190 30 173
Cuts at: 2630 2660 1178
Size: 30 4441
Fragments arranged by size:
4441 804 190 173 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2215 2335 1186
Size: 1029 120 4774
Fragments arranged by size:
4774 1029 120
BseYI C'CCAG_C
Cuts at: 2031 2716 2734 2751 5126 5622 2031
Size: 685 18 17 2375 496 2332
Fragments arranged by size:
2375 2332 685 496 18 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2374 1163
Size: 573 638 4712
Fragments arranged by size:
4712 638 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3092 3984 4069 5140 3092
Size: 892 85 1071 3875
Fragments arranged by size:
3875 1071 892 85
BsmI GAATG_Cn'
Cuts at: 2124 3434 3527 2124
Size: 1310 93 4520
Fragments arranged by size:
4520 1310 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2366 2366
Size: 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2343 2990 3092 3145 3242 3984
Size: 399 270 283 647 102 53 97 742
Cuts at: 3984 4069 5140 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 647 399 283 270 102
97 85 53
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3278 844
Size: 2434 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2882 4567 4754 1700
Size: 110 1072 1685 187 2869
Fragments arranged by size:
2869 1685 1072 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 2620 1184
Size: 1436 4487
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2264 2248
Size: 16 5907
BstYI r'GATC_y
Cuts at: 1972 2103 3253 3620 4005 4022 5474 5560 5572
Size: 131 1150 367 385 17 1452 86 12
Cuts at: 5572 5918 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1150 385 367 346 131 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2496 3108 513
Size: 552 939 492 612 3328
Fragments arranged by size:
3328 939 612 552 492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 2367 3439 4233 4253 2367
Size: 1072 794 20 4037
Fragments arranged by size:
4037 1072 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 2098 3227 2098
Size: 1129 4794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2565 3096
Size: 54 1425 50 275 18 333 402 531
Cuts at: 3096 3370 4259 5888 8
Size: 274 889 1629 43
Fragments arranged by size:
1629 1425 889 531 402 333 275 274
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2192 2396 2945 3853 1353
Size: 839 204 549 908 3423
Fragments arranged by size:
3423 908 839 549 204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2395 3986 4583 5370 5638 1239
Size: 393 530 233 1591 597 787 268 1524
Fragments arranged by size:
1591 1524 787 597 530 393 268 233
EcoO109I rG'GnC_Cy
Cuts at: 2986 2987 3142 2986
Size: 1 155 5767
Fragments arranged by size:
5767 155 1
EcoRI G'AATT_C
Cuts at: 2704 3232 3610 2704
Size: 528 378 5017
Fragments arranged by size:
5017 528 378
EcoRV GAT'ATC
Cuts at: 3115 3115
Size: 5923
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 5070 1678
Size: 3392 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2660 2692 4564 4596 4638
Size: 32 135 32 1088 32 1872 32 42
Cuts at: 4638 4670 1373
Size: 32 2626
Fragments arranged by size:
2626 1872 1088 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2165 3098 10
Size: 54 1425 343 333 933 2835
Fragments arranged by size:
2835 1425 933 343 333 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2540 3881 4240 4399 518
Size: 648 1329 45 1341 359 159 2042
Fragments arranged by size:
2042 1341 1329 648 359 159 45
MspA1I CmG'CkG
Cuts at: 1606 3339 4016 5164 5409 1606
Size: 1733 677 1148 245 2120
Fragments arranged by size:
2120 1733 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3108 513
Size: 552 939 1104 3328
Fragments arranged by size:
3328 1104 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3281 4826 1132
Size: 97 2052 1545 2229
Fragments arranged by size:
2229 2052 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3145 1348
Size: 1797 4126
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2334 2716 3109 3128 1108
Size: 1226 382 393 19 3903
Fragments arranged by size:
3903 1226 393 382 19
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2427 2459 3992 4024 5505 5537 2427
Size: 32 1533 32 1481 32 2813
Fragments arranged by size:
2813 1533 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2798 1613
Size: 1185 4738
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspOMI G'GGCC_C
Cuts at: 2986 3141 2986
Size: 155 5768
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SapI GCTCTTCn'nnn_
Cuts at: 2396 2396
Size: 5923
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 2261 4171 5883 2261
Size: 1910 1712 2301
Fragments arranged by size:
2301 1910 1712
SfcI C'TryA_G
Cuts at: 826 1045 3070 3271 4406 5087 5278 826
Size: 219 2025 201 1135 681 191 1471
Fragments arranged by size:
2025 1471 1135 681 219 201 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3149 3149
Size: 5923
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 2882 1700
Size: 1182 4741
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 4027 4928 5190 820
Size: 197 926 168 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 197 168
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 2936 1517
Size: 1419 4504
StyI C'CwwG_G
Cuts at: 513 1065 2004 2650 2937 3108 3213 3362 513
Size: 552 939 646 287 171 105 149 3074
Fragments arranged by size:
3074 939 646 552 287 171 149 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 2326 2489 3139 3950 3967 4120
Size: 1255 690 56 163 650 811 17 153
Cuts at: 4120 4305 4750 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 811 690 650 445 185 163
153 56 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 4169 5881
Size: 274 80 33 51 1443 282 1910 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1910 1712 1443 282 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2452 2484 2496 2528 2715 2747 3084 3116 5883
Size: 32 12 32 187 32 337 32 2767
Cuts at: 5883 5915 2452
Size: 32 2460
Fragments arranged by size:
2767 2460 337 187 32 32 32 32
32 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3106 3142 3106
Size: 36 5887
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2671 3731 276
Size: 53 83 186 936 1137 1060 2468
Fragments arranged by size:
2468 1137 1060 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI