Restriction map for
pFN21AB6587
(Circular) (Six-base) MAPSORT of: pFN21AB6587.seq Check: 7058 from: 1 to: 5770
pFN21AB6587 5770 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2648 3833 5217 5485 1632
Size: 1016 1185 1384 268 1917
Fragments arranged by size:
1917 1384 1185 1016 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 2809 4669 5646 1203
Size: 1606 1860 977 1327
Fragments arranged by size:
1860 1606 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 2152 3513 5085 2152
Size: 1361 1572 2837
Fragments arranged by size:
2837 1572 1361
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 3079 3305 3457 5566 1493
Size: 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2201 3095 1870
Size: 73 87 171 894 4545
Fragments arranged by size:
4545 894 171 87 73
BamHI G'GATC_C
Cuts at: 2239 2733 3100 3467 2239
Size: 494 367 367 4542
Fragments arranged by size:
4542 494 367 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3091 4546 618
Size: 325 217 627 1304 1455 1842
Fragments arranged by size:
1842 1455 1304 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2108 2406 3089 1391
Size: 717 298 683 4072
Fragments arranged by size:
4072 717 683 298
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 928
Size: 971 285 4514
Fragments arranged by size:
4514 971 285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2707 2978 3662 4872 2707
Size: 271 684 1210 3605
Fragments arranged by size:
3605 1210 684 271
BclI T'GATC_A
Cuts at: 1548 3049 1548
Size: 1501 4269
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2714 4381 136
Size: 107 122 71 631 605 1042 1667 1525
Fragments arranged by size:
1667 1525 1042 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2496 3831 4987 1790
Size: 706 1335 1156 2573
Fragments arranged by size:
2573 1335 1156 706
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2686 2723 4430 1239
Size: 845 602 37 1707 2579
Fragments arranged by size:
2579 1707 845 602 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 3895 4760 5058 2486
Size: 1409 865 298 3198
Fragments arranged by size:
3198 1409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2225 4433 882
Size: 617 726 2208 2219
Fragments arranged by size:
2219 2208 726 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2995 3577 3959 4594
Size: 53 83 186 936 1462 582 382 635
Cuts at: 4594 275
Size: 1451
Fragments arranged by size:
1462 1451 936 635 582 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 1178
Size: 30 165 30 1104 30 4411
Fragments arranged by size:
4411 1104 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 2219 2234 2383 2386 2395 1186
Size: 1018 15 15 149 3 9 4561
Fragments arranged by size:
4561 1018 149 15 15 9 3
BseYI C'CCAG_C
Cuts at: 2042 2167 2339 2723 2761 2892 4973 5469 2042
Size: 125 172 384 38 131 2081 496 2343
Fragments arranged by size:
2343 2081 496 384 172 131 125 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2108 3831 3916 4987 2108
Size: 1723 85 1071 2891
Fragments arranged by size:
2891 1723 1071 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2406 2496 3089 3831 3916 4987
Size: 399 318 298 90 593 742 85 1071
Cuts at: 4987 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 593 399 318 298 90
85
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 3125 844
Size: 1208 1073 3489
Fragments arranged by size:
3489 1208 1073
BsrBI CCG'CTC
Cuts at: 1394 1823 2458 3657 1394
Size: 429 635 1199 3507
Fragments arranged by size:
3507 1199 635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4265 4439 65
Size: 1183 3017 174 1396
Fragments arranged by size:
3017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 2313 4414 4601 1700
Size: 110 311 192 2101 187 2869
Fragments arranged by size:
2869 2101 311 192 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstXI CCAn_nnnn'nTGG
Cuts at: 2773 2920 2773
Size: 147 5623
BstYI r'GATC_y
Cuts at: 1972 2239 2733 2884 3100 3467 3852 3869 5321
Size: 267 494 151 216 367 385 17 1452
Cuts at: 5321 5407 5419 5765 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 494 385 367 346 267 216
151 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2092 2496 513
Size: 552 939 88 404 3787
Fragments arranged by size:
3787 939 552 404 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 3286 4080 4100 3286
Size: 794 20 4956
Fragments arranged by size:
4956 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2637 2841
Size: 54 1425 50 275 18 381 426 204
Cuts at: 2841 2925 2949 3217 4106 5735 8
Size: 84 24 268 889 1629 43
Fragments arranged by size:
1629 1425 889 426 381 275 268 204
84 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 2286 2360 2772 3700 1353
Size: 828 105 74 412 928 3423
Fragments arranged by size:
3423 928 828 412 105 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2392 4357 4731 4877 387
Size: 1064 138 803 1965 374 146 1280
Fragments arranged by size:
1965 1280 1064 803 374 146 138
EcoICRI GAG'CTC
Cuts at: 2106 2106
Size: 5770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2648 2686 2723 3833 4430 5217
Size: 393 452 564 38 37 1110 597 787
Cuts at: 5217 5485 1239
Size: 268 1524
Fragments arranged by size:
1524 1110 787 597 564 452 393 268
38 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2279 2978 2279
Size: 699 5071
EcoO109I rG'GnC_Cy
Cuts at: 2149 2436 2758 2785 2971 2149
Size: 287 322 27 186 4948
Fragments arranged by size:
4948 322 287 186 27
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2210 2270 2210
Size: 60 5710
FspI TGC'GCA
Cuts at: 2966 4276 2966
Size: 1310 4460
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2224 2256 2423 2455 2507
Size: 32 135 32 652 32 167 32 52
Cuts at: 2507 2539 4411 4443 4485 4517 1373
Size: 32 1872 32 42 32 2626
Fragments arranged by size:
2626 1872 652 167 135 52 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2046 2244 2841 4884 5068 1305
Size: 741 198 597 2043 184 2007
Fragments arranged by size:
2043 2007 741 597 198 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2843 2927 2951 10
Size: 54 1425 343 1011 84 24 2829
Fragments arranged by size:
2829 1425 1011 343 84 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 2489 2675 3728 4087 4246 518
Size: 648 1182 141 186 1053 359 159 2042
Fragments arranged by size:
2042 1182 1053 648 359 186 159 141
MspA1I CmG'CkG
Cuts at: 1606 2498 3186 3863 5011 5256 1606
Size: 892 688 677 1148 245 2120
Fragments arranged by size:
2120 1148 892 688 677 245
NaeI GCC'GGC
Cuts at: 2123 2315 2123
Size: 192 5578
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NgoMIV G'CCGG_C
Cuts at: 2121 2313 2121
Size: 192 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2674 2813 3128 4673 1132
Size: 97 1445 139 315 1545 2229
Fragments arranged by size:
2229 1545 1445 315 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 2809 4669 2809
Size: 1860 3910
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2152 1108
Size: 1044 4726
PfoI T'CCnGG_A
Cuts at: 2226 2274 2226
Size: 48 5722
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 2785 2149
Size: 636 5134
PshAI GACnn'nnGTC
Cuts at: 1613 2576 2994 1613
Size: 963 418 4389
Fragments arranged by size:
4389 963 418
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 3122 830
Size: 2292 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
SacI G_AGCT'C
Cuts at: 2108 2108
Size: 5770
SacII CC_GC'GG
Cuts at: 2499 2499
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SanDI GG'GwC_CC
Cuts at: 2785 2785
Size: 5770
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SfcI C'TryA_G
Cuts at: 826 1045 3118 4253 4934 5125 826
Size: 219 2073 1135 681 191 1471
Fragments arranged by size:
2073 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 3874 4775 5037 820
Size: 197 926 522 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 522 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 2674 3128 1132
Size: 1542 454 3774
Fragments arranged by size:
3774 1542 454
SspI AAT'ATT
Cuts at: 5 52 2552 3694 5
Size: 47 2500 1142 2081
Fragments arranged by size:
2500 2081 1142 47
StuI AGG'CCT
Cuts at: 1517 3061 1517
Size: 1544 4226
StyI C'CwwG_G
Cuts at: 513 1065 2004 3209 513
Size: 552 939 1205 3074
Fragments arranged by size:
3074 1205 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 2583 2895 3797 3814 3967 4152
Size: 1255 888 115 312 902 17 153 185
Cuts at: 4152 4597 325
Size: 445 1498
Fragments arranged by size:
1498 1255 902 888 445 312 185 153
115 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 4016 5728
Size: 274 80 33 51 1443 468 1571 1712
Cuts at: 5728 96
Size: 138
Fragments arranged by size:
1712 1571 1443 468 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2920 2920
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SapI
SexAI SfiI SfoI SrfI SwaI