Restriction map for
pFN21AB6586
(Circular) (Six-base) MAPSORT of: pFN21AB6586.seq Check: 1348 from: 1 to: 5923
pFN21AB6586 5923 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2824 2824
Size: 5923
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2421 3266 1937
Size: 484 845 4594
Fragments arranged by size:
4594 845 484
Acc65I G'GTAC_C
Cuts at: 2701 3244 2701
Size: 543 5380
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 3986 5370 5638 1632
Size: 701 1653 1384 268 1917
Fragments arranged by size:
1917 1653 1384 701 268
AflII C'TTAA_G
Cuts at: 820 1017 2567 2876 820
Size: 197 1550 309 3867
Fragments arranged by size:
3867 1550 309 197
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2285 1184
Size: 1101 4822
AlwNI CAG_nnn'CTG
Cuts at: 2278 3666 5238 2278
Size: 1388 1572 2963
Fragments arranged by size:
2963 1572 1388
ApaLI G'TGCA_C
Cuts at: 3047 3980 5136 3047
Size: 933 1156 3834
Fragments arranged by size:
3834 1156 933
ApoI r'AATT_y
Cuts at: 1493 2918 3232 3458 3610 5719 1493
Size: 1425 314 226 152 2109 1697
Fragments arranged by size:
2109 1697 1425 314 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 2900 3248 1870
Size: 73 133 824 348 4545
Fragments arranged by size:
4545 824 348 133 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 2654 2701 3244 4699
Size: 325 217 627 327 540 47 543 1455
Cuts at: 4699 618
Size: 1842
Fragments arranged by size:
1842 1455 627 543 540 327 325 217
47
BanII G_rGCy'C
Cuts at: 1391 2936 3242 1391
Size: 1545 306 4072
Fragments arranged by size:
4072 1545 306
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2459 2974 928
Size: 971 560 515 3877
Fragments arranged by size:
3877 971 560 515
BbvCI CC'TCA_GC
Cuts at: 2864 2864
Size: 5923
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2567 2572 2601 2606 4114
Size: 34 50 34 546 5 29 5 1508
Cuts at: 4114 4148 1903
Size: 34 3678
Fragments arranged by size:
3678 1508 546 50 34 34 34 29
5 5
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 2595 1548
Size: 1047 4876
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2123 4534 136
Size: 107 122 71 631 605 451 2411 1525
Fragments arranged by size:
2411 1525 631 605 451 122 107 71
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 2384 3340 2384
Size: 956 4967
Bme1580I G_kGCm'C
Cuts at: 1790 3051 3984 5140 1790
Size: 1261 933 1156 2573
Fragments arranged by size:
2573 1261 1156 933
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2808 2955 3654
Size: 416 53 83 399 24 1391 147 699
Cuts at: 3654 4612 442
Size: 958 1753
Fragments arranged by size:
1753 1391 958 699 416 399 147 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2300 2966 4583 1239
Size: 1046 15 666 1617 2579
Fragments arranged by size:
2579 1617 1046 666 15
Bpu10I CC'TnA_GC
Cuts at: 2864 2864
Size: 5923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2677 3714 493
Size: 711 1473 1037 2702
Fragments arranged by size:
2702 1473 1037 711
BsaBI GATnn'nnATC
Cuts at: 2273 2727 5917 2273
Size: 454 3190 2279
Fragments arranged by size:
3190 2279 454
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3730 4112 4747 275
Size: 53 83 186 936 2197 382 635 1451
Fragments arranged by size:
2197 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2745 4356 4754 5028 5175 5586 2745
Size: 1611 398 274 147 411 3082
Fragments arranged by size:
3082 1611 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5698
Fragments arranged by size:
5698 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2009 1186
Size: 823 5100
BseYI C'CCAG_C
Cuts at: 2129 2186 2252 5126 5622 2129
Size: 57 66 2874 496 2430
Fragments arranged by size:
2874 2430 496 66 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 1163
Size: 573 573 4777
Fragments arranged by size:
4777 573 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2592 3373 4134 5162 1540
Size: 275 186 591 781 761 1028 2301
Fragments arranged by size:
2301 1028 781 761 591 275 186
BsiHKAI G_wGCw'C
Cuts at: 3051 3984 4069 5140 3051
Size: 933 85 1071 3834
Fragments arranged by size:
3834 1071 933 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2936 3051 3242 3984 4069 5140 1391
Size: 399 1146 115 191 742 85 1071 2174
Fragments arranged by size:
2174 1146 1071 742 399 191 115 85
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2433 2824 3278 844
Size: 1589 391 454 3489
Fragments arranged by size:
3489 1589 454 391
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2401 2590 2794 4418 4592 65
Size: 1183 1153 189 204 1624 174 1396
Fragments arranged by size:
1624 1396 1183 1153 204 189 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4567 4754 1700
Size: 110 2757 187 2869
Fragments arranged by size:
2869 2757 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2230
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2274 3253 3620 4005 4022 5474 5560 5572
Size: 302 979 367 385 17 1452 86 12
Cuts at: 5572 5918 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 979 385 367 346 302 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2046 2046
Size: 5923
BtgI C'CryG_G
Cuts at: 513 1065 2118 2793 3099 513
Size: 552 1053 675 306 3337
Fragments arranged by size:
3337 1053 675 552 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 3439 4233 4253 3439
Size: 794 20 5109
Fragments arranged by size:
5109 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 2620 3227 2620
Size: 607 5316
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 3370 4259
Size: 54 1425 50 275 18 402 1138 889
Cuts at: 4259 5888 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1138 889 402 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2084 3203 3853 1353
Size: 665 66 1119 650 3423
Fragments arranged by size:
3423 1119 665 650 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2300 2333 2966 3986 4583 5370
Size: 393 653 15 33 633 1020 597 787
Cuts at: 5370 5638 1239
Size: 268 1524
Fragments arranged by size:
1524 1020 787 653 633 597 393 268
33 15
EcoO109I rG'GnC_Cy
Cuts at: 2052 2101 2166 2284 2773 3162 2052
Size: 49 65 118 489 389 4813
Fragments arranged by size:
4813 489 389 118 65 49
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 5070 1678
Size: 3392 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373
Size: 32 135 32 2992 32 42 32 2626
Fragments arranged by size:
2992 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2638 748
Size: 1890 4033
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 2705 3248 2705
Size: 543 5380
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 10
Size: 54 1425 343 402 3699
Fragments arranged by size:
3699 1425 402 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 2222 2267 3881 4240 4399 518
Size: 648 946 110 45 1614 359 159 2042
Fragments arranged by size:
2042 1614 946 648 359 159 110 45
MspA1I CmG'CkG
Cuts at: 1606 2180 2383 2944 3092 3339 4016 5164 5409
Size: 574 203 561 148 247 677 1148 245
Cuts at: 5409 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 574 561 247 245 203
148
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5371
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3281 4826 1132
Size: 97 2052 1545 2229
Fragments arranged by size:
2229 2052 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2245 1348
Size: 897 5026
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5923
PfoI T'CCnGG_A
Cuts at: 2277 2739 2277
Size: 462 5461
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2773 2101
Size: 672 5251
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspXI vC'TCGA_Gb
Cuts at: 2900 2900
Size: 5923
PstI C_TGCA'G
Cuts at: 830 2430 2503 2762 3275 830
Size: 1600 73 259 513 3478
Fragments arranged by size:
3478 1600 513 259 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2383 2944 2383
Size: 561 5362
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SanDI GG'GwC_CC
Cuts at: 2773 2773
Size: 5923
SapI GCTCTTCn'nnn_
Cuts at: 2018 2018
Size: 5923
SbfI CC_TGCA'GG
Cuts at: 2430 2762 3275 2430
Size: 332 513 5078
Fragments arranged by size:
5078 513 332
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SexAI A'CCwGG_T
Cuts at: 2412 2412
Size: 5923
SfcI C'TryA_G
Cuts at: 826 1045 2161 2426 2499 2758 3271 4406 5087
Size: 219 1116 265 73 259 513 1135 681
Cuts at: 5087 5278 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1116 681 513 265 259 219
191 73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 2078 3250 1872
Size: 206 1172 4545
Fragments arranged by size:
4545 1172 206
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2567 2876 2900 4027 4928 5190
Size: 197 926 624 309 24 1127 901 262
Cuts at: 5190 820
Size: 1553
Fragments arranged by size:
1553 1127 926 901 624 309 262 197
24
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3111 3847 5
Size: 47 3059 736 2081
Fragments arranged by size:
3059 2081 736 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2169 2782 3202 3362 513
Size: 552 1104 613 420 160 3074
Fragments arranged by size:
3074 1104 613 552 420 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2672 3950 3967 4120 4305 4750 325
Size: 1255 1092 1278 17 153 185 445 1498
Fragments arranged by size:
1498 1278 1255 1092 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4169 5881 96
Size: 274 80 33 51 1443 2192 1712 138
Fragments arranged by size:
2192 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2242 2369 2242
Size: 127 5796
XhoI C'TCGA_G
Cuts at: 1943 2900 1943
Size: 957 4966
XmaI C'CCGG_G
Cuts at: 1870 2076 3248 1870
Size: 206 1172 4545
Fragments arranged by size:
4545 1172 206
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII
SfiI SfoI SrfI SwaI