Restriction map for
pFN21AB6583
(Circular) (Six-base) MAPSORT of: pFN21AB6583.seq Check: 2437 from: 1 to: 5836
pFN21AB6583 5836 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3073 3073
Size: 5836
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2919 3646 278
Size: 53 83 186 936 1383 727 2468
Fragments arranged by size:
2468 1383 936 727 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3899 5283 5551 1632
Size: 2267 1384 268 1917
Fragments arranged by size:
2267 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 2099 2258 1166
Size: 933 159 4744
Fragments arranged by size:
4744 933 159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 2530 3579 5151 2530
Size: 1049 1572 3215
Fragments arranged by size:
3215 1572 1049
ApaI G_GGCC'C
Cuts at: 2249 2888 2249
Size: 639 5197
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 2116 3145 3371 3523 5632 1493
Size: 623 1029 226 152 2109 1697
Fragments arranged by size:
2109 1697 1029 623 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 2121 2852 3104 3161 1870
Size: 73 133 45 731 252 57 4545
Fragments arranged by size:
4545 731 252 133 73 57 45
AvrII C'CTAG_G
Cuts at: 3031 3031
Size: 5836
BamHI G'GATC_C
Cuts at: 2610 3166 3533 2610
Size: 556 367 4913
Fragments arranged by size:
4913 556 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 3110 3157 4612 618
Size: 325 217 627 546 777 47 1455 1842
Fragments arranged by size:
1842 1455 777 627 546 325 217 47
BanII G_rGCy'C
Cuts at: 1391 2138 2249 2861 2888 3155 1391
Size: 747 111 612 27 267 4072
Fragments arranged by size:
4072 747 612 267 111 27
BbeI G_GCGC'C
Cuts at: 2337 3114 2337
Size: 777 5059
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4027 4061 1903
Size: 34 2090 34 3678
Fragments arranged by size:
3678 2090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2856 4447 136
Size: 107 122 71 631 605 1184 1591 1525
Fragments arranged by size:
1591 1525 1184 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 3253 3253
Size: 5836
Bme1580I G_kGCm'C
Cuts at: 1790 2219 2249 2888 3897 5053 1790
Size: 429 30 639 1009 1156 2573
Fragments arranged by size:
2573 1156 1009 639 429 30
BmgBI CAC'GTC
Cuts at: 1318 2820 1318
Size: 1502 4334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2729 3567 4525
Size: 416 53 83 399 24 1312 838 958
Cuts at: 4525 442
Size: 1753
Fragments arranged by size:
1753 1312 958 838 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 2459 2636 2740 4496 1239
Size: 798 422 177 104 1756 2579
Fragments arranged by size:
2579 1756 798 422 177 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2624 3961 4826 5124 2624
Size: 1337 865 298 3336
Fragments arranged by size:
3336 1337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2462 4499 882
Size: 617 963 2037 2219
Fragments arranged by size:
2219 2037 963 617
BsaAI yAC'GTr
Cuts at: 493 1204 3627 493
Size: 711 2423 2702
Fragments arranged by size:
2702 2423 711
BsaBI GATnn'nnATC
Cuts at: 5830 5830
Size: 5836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2334 2916 3111
Size: 53 83 186 936 476 325 582 195
Cuts at: 3111 3643 4025 4660 275
Size: 532 382 635 1451
Fragments arranged by size:
1451 936 635 582 532 476 382 325
195 186 83 53
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5611
Fragments arranged by size:
5611 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2966 2969 2996 3005 3035 1186
Size: 1780 3 27 9 30 3987
Fragments arranged by size:
3987 1780 30 27 9 3
BseYI C'CCAG_C
Cuts at: 2248 2881 5039 5535 2248
Size: 633 2158 496 2549
Fragments arranged by size:
2549 2158 633 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3059 1163
Size: 573 1323 3940
Fragments arranged by size:
3940 1323 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2419 2769 3286 4047 5075 1540
Size: 275 186 418 350 517 761 1028 2301
Fragments arranged by size:
2301 1028 761 517 418 350 275 186
BsiHKAI G_wGCw'C
Cuts at: 3897 3982 5053 3897
Size: 85 1071 4680
Fragments arranged by size:
4680 1071 85
BsmI GAATG_Cn'
Cuts at: 2156 3347 3440 2156
Size: 1191 93 4552
Fragments arranged by size:
4552 1191 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2219 2249 2861 2888 3155 3897
Size: 399 348 81 30 612 27 267 742
Cuts at: 3897 3982 5053 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 612 399 348 267 85
81 30 27
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2403 3073 3191 844
Size: 1559 670 118 3489
Fragments arranged by size:
3489 1559 670 118
BsrBI CCG'CTC
Cuts at: 1394 1823 3130 3723 1394
Size: 429 1307 593 3507
Fragments arranged by size:
3507 1307 593 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2358 4331 4505 65
Size: 1183 1110 1973 174 1396
Fragments arranged by size:
1973 1396 1183 1110 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2453 4480 4667 1700
Size: 110 643 2027 187 2869
Fragments arranged by size:
2869 2027 643 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 2570 3896 4908 2570
Size: 1326 1012 3498
Fragments arranged by size:
3498 1326 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2360 794
Size: 1566 4270
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 2866 1184
Size: 1682 4154
BstXI CCAn_nnnn'nTGG
Cuts at: 2260 2350 2260
Size: 90 5746
BstYI r'GATC_y
Cuts at: 1972 2553 2610 2715 2730 3166 3533 3918 3935
Size: 581 57 105 15 436 367 385 17
Cuts at: 3935 5387 5473 5485 5831 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 581 436 385 367 346 105
86 57 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 2862 513
Size: 552 939 90 768 3487
Fragments arranged by size:
3487 939 768 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2106 2217 3352 4146 4166 2106
Size: 111 1135 794 20 3776
Fragments arranged by size:
3776 1135 794 111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 2676 3140 2676
Size: 464 5372
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2340 2416 2577
Size: 54 1425 50 275 18 510 76 161
Cuts at: 2577 2766 3283 4172 5801 8
Size: 189 517 889 1629 43
Fragments arranged by size:
1629 1425 889 517 510 275 189 161
76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2416 2766 3283 1537
Size: 275 604 350 517 4090
Fragments arranged by size:
4090 604 517 350 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 2954 3766 1353
Size: 828 773 812 3423
Fragments arranged by size:
3423 828 812 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2117 4423 4797 4943 387
Size: 1064 138 528 2306 374 146 1280
Fragments arranged by size:
2306 1280 1064 528 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2459 2636 2740 3899 4496 5283
Size: 393 405 422 177 104 1159 597 787
Cuts at: 5283 5551 1239
Size: 268 1524
Fragments arranged by size:
1524 1159 787 597 422 405 393 268
177 104
EcoO109I rG'GnC_Cy
Cuts at: 2885 2885
Size: 5836
EcoRI G'AATT_C
Cuts at: 2116 3145 3523 2116
Size: 1029 378 4429
Fragments arranged by size:
4429 1029 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2687 2687
Size: 5836
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 2337 3114 4983 1678
Size: 659 777 1869 2531
Fragments arranged by size:
2531 1869 777 659
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2222 2254 2479 2511 2804
Size: 32 135 32 650 32 225 32 293
Cuts at: 2804 2836 4477 4509 4551 4583 1373
Size: 32 1641 32 42 32 2626
Fragments arranged by size:
2626 1641 650 293 225 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KasI G'GCGC_C
Cuts at: 2333 3110 2333
Size: 777 5059
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 10
Size: 54 1425 343 510 3504
Fragments arranged by size:
3504 1425 510 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2258 2348 3794 4153 4312 518
Size: 648 933 159 90 1446 359 159 2042
Fragments arranged by size:
2042 1446 933 648 359 159 159 90
MspA1I CmG'CkG
Cuts at: 1606 2086 2437 2582 3252 3929 5077 5322 1606
Size: 480 351 145 670 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 670 480 351 245 145
NarI GG'CG_CC
Cuts at: 2334 3111 2334
Size: 777 5059
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4345
Fragments arranged by size:
4345 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5836
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2575 2607 2881 1108
Size: 1467 32 274 4063
Fragments arranged by size:
4063 1467 274 32
PfoI T'CCnGG_A
Cuts at: 2053 2523 2556 2613 2053
Size: 470 33 57 5276
Fragments arranged by size:
5276 470 57 33
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PspOMI G'GGCC_C
Cuts at: 2245 2884 2245
Size: 639 5197
PstI C_TGCA'G
Cuts at: 830 3188 830
Size: 2358 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2437 2437
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 2111 3184 4319 5000 5191 826
Size: 219 1066 1073 1135 681 191 1471
Fragments arranged by size:
1471 1135 1073 1066 681 219 191
SfoI GGC'GCC
Cuts at: 2335 3112 2335
Size: 777 5059
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 2123 3163 1872
Size: 251 1040 4545
Fragments arranged by size:
4545 1040 251
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2076 2639 3940 4841 5103 820
Size: 197 926 133 563 1301 901 262 1553
Fragments arranged by size:
1553 1301 926 901 563 262 197 133
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2586 3031 3275 513
Size: 552 939 237 345 445 244 3074
Fragments arranged by size:
3074 939 552 445 345 244 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3863 3880 4033 4218 4663 325
Size: 1255 2283 17 153 185 445 1498
Fragments arranged by size:
2283 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4082 5794 96
Size: 274 80 33 51 1443 2105 1712 138
Fragments arranged by size:
2105 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5796 5828 5796
Size: 32 5804
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2611 2611
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 2076 1943
Size: 133 5703
XmaI C'CCGG_G
Cuts at: 1870 2121 3161 1870
Size: 251 1040 4545
Fragments arranged by size:
4545 1040 251
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2917 3644 276
Size: 53 83 186 936 1383 727 2468
Fragments arranged by size:
2468 1383 936 727 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI