Restriction map for
pFN21AB6582
(Circular) (Six-base) MAPSORT of: pFN21AB6582.seq Check: 5915 from: 1 to: 5917
pFN21AB6582 5917 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3260 1937
Size: 1323 4594
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 3980 5364 5632 1632
Size: 746 1602 1384 268 1917
Fragments arranged by size:
1917 1602 1384 746 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 4816 5793 1203
Size: 3613 977 1327
Fragments arranged by size:
3613 1327 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 2239 4646 2239
Size: 2407 3510
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AlwNI CAG_nnn'CTG
Cuts at: 2179 3660 5232 2179
Size: 1481 1572 2864
Fragments arranged by size:
2864 1572 1481
ApaI G_GGCC'C
Cuts at: 3123 3123
Size: 5917
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 2096 3226 3452 3604 5713 1493
Size: 603 1130 226 152 2109 1697
Fragments arranged by size:
2109 1697 1130 603 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2650 3209 3242 1870
Size: 73 707 559 33 4545
Fragments arranged by size:
4545 707 559 73 33
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3238 4693 618
Size: 325 217 627 1451 1455 1842
Fragments arranged by size:
1842 1455 1451 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 3123 3236 1391
Size: 643 1089 113 4072
Fragments arranged by size:
4072 1089 643 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4946
BbvCI CC'TCA_GC
Cuts at: 3052 3052
Size: 5917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 2434 1548
Size: 886 5031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4528 136
Size: 107 122 71 631 605 2856 1525
Fragments arranged by size:
2856 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2844 5912 2844
Size: 3068 2849
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 2784 3123 3978 5134 1790
Size: 994 339 855 1156 2573
Fragments arranged by size:
2573 1156 994 855 339
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4606 442
Size: 416 53 83 399 24 2231 958 1753
Fragments arranged by size:
2231 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3172 4577 1239
Size: 1933 1405 2579
Fragments arranged by size:
2579 1933 1405
Bpu10I CC'TnA_GC
Cuts at: 3052 3052
Size: 5917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2436 4042 4907 5205 2436
Size: 1606 865 298 3148
Fragments arranged by size:
3148 1606 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2306 4580 882
Size: 617 807 2274 2219
Fragments arranged by size:
2274 2219 807 617
BsaAI yAC'GTr
Cuts at: 493 1204 3708 493
Size: 711 2504 2702
Fragments arranged by size:
2702 2504 711
BsaBI GATnn'nnATC
Cuts at: 2346 5911 2346
Size: 3565 2352
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 3724 4106 4741
Size: 53 83 186 936 690 1501 382 635
Cuts at: 4741 275
Size: 1451
Fragments arranged by size:
1501 1451 936 690 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4350 4748 5022 5169 5580 4350
Size: 398 274 147 411 4687
Fragments arranged by size:
4687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5692
Fragments arranged by size:
5692 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 1186
Size: 1075 4842
BseYI C'CCAG_C
Cuts at: 2140 5120 5616 2140
Size: 2980 496 2441
Fragments arranged by size:
2980 2441 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 4128 5156 1540
Size: 275 186 1366 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3978 4063 5134 3978
Size: 85 1071 4761
Fragments arranged by size:
4761 1071 85
BsmI GAATG_Cn'
Cuts at: 3101 3428 3521 3101
Size: 327 93 5497
Fragments arranged by size:
5497 327 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2784 3123 3236 3978 4063 5134
Size: 399 244 750 339 113 742 85 1071
Cuts at: 5134 1391
Size: 2174
Fragments arranged by size:
2174 1071 750 742 399 339 244 113
85
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3135 3806 5536 3135
Size: 671 1730 3516
Fragments arranged by size:
3516 1730 671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3205 3272 844
Size: 2361 67 3489
Fragments arranged by size:
3489 2361 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2589 3804 1394
Size: 429 766 1215 3507
Fragments arranged by size:
3507 1215 766 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4412 4586 65
Size: 1183 3164 174 1396
Fragments arranged by size:
3164 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4561 4748 1700
Size: 110 2751 187 2869
Fragments arranged by size:
2869 2751 187 110
BsrGI T'GTAC_A
Cuts at: 96 2640 3094 96
Size: 2544 454 2919
Fragments arranged by size:
2919 2544 454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 3028 1184
Size: 1844 4073
BstYI r'GATC_y
Cuts at: 1972 2019 2347 2844 3196 3247 3614 3999 4016
Size: 47 328 497 352 51 367 385 17
Cuts at: 4016 5468 5554 5566 5912 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 497 385 367 352 346 328
86 51 47 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2611 513
Size: 552 1546 3819
Fragments arranged by size:
3819 1546 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 2109 3433 4227 4247 2109
Size: 1324 794 20 3779
Fragments arranged by size:
3779 1324 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2944 2979 529
Size: 35 2380 35 3467
Fragments arranged by size:
3467 2380 35 35
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4924 809
Size: 507 3608 1802
Fragments arranged by size:
3608 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3364 4253 5882
Size: 54 1425 50 275 18 1534 889 1629
Cuts at: 5882 8
Size: 43
Fragments arranged by size:
1629 1534 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2328 2741 3134 3847 1353
Size: 975 413 393 713 3423
Fragments arranged by size:
3423 975 713 413 393
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 3172 3980 4577 5364 5632 1239
Size: 393 746 794 808 597 787 268 1524
Fragments arranged by size:
1524 808 794 787 746 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2126 3212 2126
Size: 1086 4831
EcoO109I rG'GnC_Cy
Cuts at: 3119 3120 3119
Size: 1 5916
EcoRI G'AATT_C
Cuts at: 2096 3226 3604 2096
Size: 1130 378 4409
Fragments arranged by size:
4409 1130 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2705 2705
Size: 5917
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 5064 1678
Size: 3386 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2286 2318 2829
Size: 32 135 32 653 32 29 32 511
Cuts at: 2829 2861 4558 4590 4632 4664 1373
Size: 32 1697 32 42 32 2626
Fragments arranged by size:
2626 1697 653 511 135 42 32 32
32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 3507 669
Size: 988 281 1323 246 3079
Fragments arranged by size:
3079 1323 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2400 748
Size: 1652 4265
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 3046 5031 5215 1305
Size: 918 823 1985 184 2007
Fragments arranged by size:
2007 1985 918 823 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4095
Fragments arranged by size:
4095 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3875 4234 4393 518
Size: 648 2709 359 159 2042
Fragments arranged by size:
2709 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2991 3169 3333 4010 5158 5403 1606
Size: 1385 178 164 677 1148 245 2120
Fragments arranged by size:
2120 1385 1148 677 245 178 164
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5365
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 2501 3275 4820 1132
Size: 97 1272 774 1545 2229
Fragments arranged by size:
2229 1545 1272 774 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2179 1108
Size: 1071 4846
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PspOMI G'GGCC_C
Cuts at: 3119 3119
Size: 5917
PstI C_TGCA'G
Cuts at: 830 2049 3269 830
Size: 1219 1220 3478
Fragments arranged by size:
3478 1220 1219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
PvuII CAG'CTG
Cuts at: 3169 3169
Size: 5917
RsrII CG'GwC_CG
Cuts at: 2209 2209
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 2111 3008 4165 5877 2111
Size: 897 1157 1712 2151
Fragments arranged by size:
2151 1712 1157 897
SfcI C'TryA_G
Cuts at: 826 1045 2045 3265 4400 5081 5272 826
Size: 219 1000 1220 1135 681 191 1471
Fragments arranged by size:
1471 1220 1135 1000 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3244 1872
Size: 1372 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2451 4021 4922 5184 820
Size: 197 926 508 1570 901 262 1553
Fragments arranged by size:
1570 1553 926 901 508 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 2351 2509 3356 513
Size: 552 1286 158 847 3074
Fragments arranged by size:
3074 1286 847 552 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3944 3961 4114 4299 4744 325
Size: 1255 2364 17 153 185 445 1498
Fragments arranged by size:
2364 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2109 2640 3006
Size: 274 80 33 51 1443 132 531 366
Cuts at: 3006 3094 4163 5875 96
Size: 88 1069 1712 138
Fragments arranged by size:
1712 1443 1069 531 366 274 138 132
88 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 2090 2213 1316
Size: 774 123 5020
Fragments arranged by size:
5020 774 123
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2516 3196 2516
Size: 680 5237
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 3242 1870
Size: 1372 4545
XmnI GAAnn'nnTTC
Cuts at: 4046 4046
Size: 5917
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI