Restriction map for
pFN21AB6581
(Circular) (Six-base) MAPSORT of: pFN21AB6581.seq Check: 9303 from: 1 to: 5836
pFN21AB6581 5836 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2970 3646 278
Size: 53 83 186 936 1434 676 2468
Fragments arranged by size:
2468 1434 936 676 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 2698 3963 4336 1563
Size: 1135 1265 373 3063
Fragments arranged by size:
3063 1265 1135 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2219 3899 5283 5551 1632
Size: 587 1680 1384 268 1917
Fragments arranged by size:
1917 1680 1384 587 268
AflII C'TTAA_G
Cuts at: 820 1017 2959 820
Size: 197 1942 3697
Fragments arranged by size:
3697 1942 197
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 3579 5151 3579
Size: 1572 4264
ApaI G_GGCC'C
Cuts at: 2392 2392
Size: 5836
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 2504 2756 2936 2975 3145 3371 3523 5632
Size: 1011 252 180 39 170 226 152 2109
Cuts at: 5632 1493
Size: 1697
Fragments arranged by size:
2109 1697 1011 252 226 180 170 152
39
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2121 2310 2385 3161 1870
Size: 73 178 189 75 776 4545
Fragments arranged by size:
4545 776 189 178 75 73
BamHI G'GATC_C
Cuts at: 2947 3166 3533 2947
Size: 219 367 5250
Fragments arranged by size:
5250 367 219
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3157 4612 618
Size: 325 217 627 1370 1455 1842
Fragments arranged by size:
1842 1455 1370 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2392 3155 1391
Size: 1001 763 4072
Fragments arranged by size:
4072 1001 763
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2611 928
Size: 971 712 4153
Fragments arranged by size:
4153 971 712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4027 4061 1903
Size: 34 2090 34 3678
Fragments arranged by size:
3678 2090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3088 3728 4938 3088
Size: 640 1210 3986
Fragments arranged by size:
3986 1210 640
BclI T'GATC_A
Cuts at: 1548 2280 1548
Size: 732 5104
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2396 4447 136
Size: 107 122 71 631 605 724 2051 1525
Fragments arranged by size:
2051 1525 724 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 3253 3253
Size: 5836
Bme1580I G_kGCm'C
Cuts at: 1790 2392 3897 5053 1790
Size: 602 1505 1156 2573
Fragments arranged by size:
2573 1505 1156 602
BmgBI CAC'GTC
Cuts at: 1318 3088 1318
Size: 1770 4066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2522 3567 4525
Size: 416 53 83 399 24 1105 1045 958
Cuts at: 4525 442
Size: 1753
Fragments arranged by size:
1753 1105 1045 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4496 1239
Size: 3257 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2734 2912 3101 3961 4826 5124 2734
Size: 178 189 860 865 298 3446
Fragments arranged by size:
3446 865 860 298 189 178
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4499 882
Size: 617 3000 2219
Fragments arranged by size:
3000 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3627 493
Size: 711 2423 2702
Fragments arranged by size:
2702 2423 711
BsaBI GATnn'nnATC
Cuts at: 2210 5830 2210
Size: 3620 2216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2497 2967 3643 4025
Size: 53 83 186 936 964 470 676 382
Cuts at: 4025 4660 275
Size: 635 1451
Fragments arranged by size:
1451 964 936 676 635 470 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2994 4269 4667 4941 5088 5499 2994
Size: 1275 398 274 147 411 3331
Fragments arranged by size:
3331 1275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5611
Fragments arranged by size:
5611 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 2909 1186
Size: 1078 645 4113
Fragments arranged by size:
4113 1078 645
BseYI C'CCAG_C
Cuts at: 2164 5039 5535 2164
Size: 2875 496 2465
Fragments arranged by size:
2875 2465 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3897 3982 5053 3897
Size: 85 1071 4680
Fragments arranged by size:
4680 1071 85
BsmI GAATG_Cn'
Cuts at: 2488 3347 3440 2488
Size: 859 93 4884
Fragments arranged by size:
4884 859 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2958 2958
Size: 5836
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2392 3155 3897 3982 5053 1391
Size: 399 602 763 742 85 1071 2174
Fragments arranged by size:
2174 1071 763 742 602 399 85
BspEI T'CCGG_A
Cuts at: 2994 5499 2994
Size: 2505 3331
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3191 844
Size: 2347 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2181 3723 1394
Size: 429 358 1542 3507
Fragments arranged by size:
3507 1542 429 358
BsrDI GCAATG_nn'
Cuts at: 65 1248 2857 4331 4505 65
Size: 1183 1609 1474 174 1396
Fragments arranged by size:
1609 1474 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2493 4480 4667 1700
Size: 110 683 1987 187 2869
Fragments arranged by size:
2869 1987 683 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 2239 2241 2243 1925
Size: 314 2 2 5518
Fragments arranged by size:
5518 314 2 2
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3127 794
Size: 2333 3503
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 2357 2357
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 2154 2947 3166 3533 3918 3935 5387 5473
Size: 182 793 219 367 385 17 1452 86
Cuts at: 5473 5485 5831 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 793 385 367 346 219 182
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2218 2394 513
Size: 552 939 214 176 3955
Fragments arranged by size:
3955 939 552 214 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2953 3045 3352 4146 4166 2953
Size: 92 307 794 20 4623
Fragments arranged by size:
4623 794 307 92 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3283 4172 5801
Size: 54 1425 50 275 18 1453 889 1629
Cuts at: 5801 8
Size: 43
Fragments arranged by size:
1629 1453 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3766 1353
Size: 2413 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2246 3059 4423 4797 4943
Size: 1064 138 435 222 813 1364 374 146
Cuts at: 4943 387
Size: 1280
Fragments arranged by size:
1364 1280 1064 813 435 374 222 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 3899 4496 5283 5551 1239
Size: 393 587 1680 597 787 268 1524
Fragments arranged by size:
1680 1524 787 597 587 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2389 2389
Size: 5836
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2902 2209
Size: 693 5143
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 4983 1678
Size: 3305 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373
Size: 32 135 32 2905 32 42 32 2626
Fragments arranged by size:
2905 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2318 748
Size: 1570 4266
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4014
Fragments arranged by size:
4014 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3794 4153 4312 518
Size: 648 2628 359 159 2042
Fragments arranged by size:
2628 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2472 3252 3929 5077 5322 1606
Size: 866 780 677 1148 245 2120
Fragments arranged by size:
2120 1148 866 780 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2394 513
Size: 552 939 390 3955
Fragments arranged by size:
3955 939 552 390
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2331 1348
Size: 983 4853
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2410 1108
Size: 1302 4534
PfoI T'CCnGG_A
Cuts at: 2535 2535
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2496 1613
Size: 883 4953
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PspOMI G'GGCC_C
Cuts at: 2388 2388
Size: 5836
PstI C_TGCA'G
Cuts at: 830 3188 830
Size: 2358 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SexAI A'CCwGG_T
Cuts at: 2763 2763
Size: 5836
SfcI C'TryA_G
Cuts at: 826 1045 2269 3131 3184 4319 5000 5191 826
Size: 219 1224 862 53 1135 681 191 1471
Fragments arranged by size:
1471 1224 1135 862 681 219 191 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 2387 3163 1872
Size: 515 776 4545
Fragments arranged by size:
4545 776 515
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2310 2749 2891 2959 3116 3940
Size: 197 926 367 439 142 68 157 824
Cuts at: 3940 4841 5103 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 824 439 367 262 197
157 142 68
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 2531 3760 5
Size: 47 2479 1229 2081
Fragments arranged by size:
2479 2081 1229 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2394 2404 3275 513
Size: 552 939 93 297 10 871 3074
Fragments arranged by size:
3074 939 871 552 297 93 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2504 3863 3880 4033 4218 4663 325
Size: 1255 924 1359 17 153 185 445 1498
Fragments arranged by size:
1498 1359 1255 924 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4082 5794 96
Size: 274 80 33 51 1443 2105 1712 138
Fragments arranged by size:
2105 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5796 5828 5796
Size: 32 5804
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 2310 1943
Size: 367 5469
XmaI C'CCGG_G
Cuts at: 1870 2385 3161 1870
Size: 515 776 4545
Fragments arranged by size:
4545 776 515
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2968 3644 276
Size: 53 83 186 936 1434 676 2468
Fragments arranged by size:
2468 1434 936 676 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI RsrII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI