Restriction map for
pFN21AB6580
(Circular) (Six-base) MAPSORT of: pFN21AB6580.seq Check: 8003 from: 1 to: 5914
pFN21AB6580 5914 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3724 278
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2139 3257 1937
Size: 202 1118 4594
Fragments arranged by size:
4594 1118 202
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3977 5361 5629 1632
Size: 2345 1384 268 1917
Fragments arranged by size:
2345 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 4813 5790 1203
Size: 3610 977 1327
Fragments arranged by size:
3610 1327 977
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 4643 4643
Size: 5914
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5914
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2623 2655 2699 2731 2623
Size: 32 44 32 5806
Fragments arranged by size:
5806 44 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3657 5229 3657
Size: 1572 4342
ApaLI G'TGCA_C
Cuts at: 3971 5127 3971
Size: 1156 4758
ApoI r'AATT_y
Cuts at: 1493 2243 2435 3022 3223 3449 3601 5710 1493
Size: 750 192 587 201 226 152 2109 1697
Fragments arranged by size:
2109 1697 750 587 226 201 192 152
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 3239 1870
Size: 73 1296 4545
Fragments arranged by size:
4545 1296 73
BamHI G'GATC_C
Cuts at: 3244 3611 3244
Size: 367 5547
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3235 4690 618
Size: 325 217 627 1448 1455 1842
Fragments arranged by size:
1842 1455 1448 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3233 1391
Size: 1842 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2283 2715 928
Size: 971 384 432 4127
Fragments arranged by size:
4127 971 432 384
BbvCI CC'TCA_GC
Cuts at: 2736 2736
Size: 5914
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4105 4139 1903
Size: 34 2168 34 3678
Fragments arranged by size:
3678 2168 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 2893 3127 1548
Size: 1345 234 4335
Fragments arranged by size:
4335 1345 234
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4525 136
Size: 107 122 71 631 605 2853 1525
Fragments arranged by size:
2853 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2323 5909 2323
Size: 3586 2328
BlpI GC'TnA_GC
Cuts at: 3331 3331
Size: 5914
Bme1580I G_kGCm'C
Cuts at: 1790 3975 5131 1790
Size: 2185 1156 2573
Fragments arranged by size:
2573 2185 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5914
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2505 3645 4603
Size: 416 53 83 399 24 1088 1140 958
Cuts at: 4603 442
Size: 1753
Fragments arranged by size:
1753 1140 1088 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3084 1056
Size: 2028 3886
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4574 1239
Size: 3335 2579
Bpu10I CC'TnA_GC
Cuts at: 2736 2736
Size: 5914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4039 4904 5202 4039
Size: 865 298 4751
Fragments arranged by size:
4751 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4577 882
Size: 617 3078 2219
Fragments arranged by size:
3078 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 2352 3186 5908 2352
Size: 834 2722 2358
Fragments arranged by size:
2722 2358 834
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3721 4103 4738 275
Size: 53 83 186 936 2188 382 635 1451
Fragments arranged by size:
2188 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4347 4745 5019 5166 5577 4347
Size: 398 274 147 411 4684
Fragments arranged by size:
4684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5689
Fragments arranged by size:
5689 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3074 1186
Size: 1888 4026
BseYI C'CCAG_C
Cuts at: 5117 5613 5117
Size: 496 5418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2949 1163
Size: 573 1213 4128
Fragments arranged by size:
4128 1213 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3364 4125 5153 1540
Size: 275 186 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3975 4060 5131 3975
Size: 85 1071 4758
Fragments arranged by size:
4758 1071 85
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3233 3975 4060 5131 1391
Size: 399 1443 742 85 1071 2174
Fragments arranged by size:
2174 1443 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3269 844
Size: 2425 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3801 1394
Size: 429 1978 3507
Fragments arranged by size:
3507 1978 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4409 4583 65
Size: 1183 3161 174 1396
Fragments arranged by size:
3161 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4558 4745 1700
Size: 110 2748 187 2869
Fragments arranged by size:
2869 2748 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5914
BssSI C'ACGA_G
Cuts at: 3974 4986 3974
Size: 1012 4902
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5914
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 2323 3244 3611 3996 4013 5465 5551 5563
Size: 351 921 367 385 17 1452 86 12
Cuts at: 5563 5909 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 921 385 367 351 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2140 2140
Size: 5914
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5175
Fragments arranged by size:
5175 683 56
BtsI GCAGTG_nn'
Cuts at: 3430 4224 4244 3430
Size: 794 20 5100
Fragments arranged by size:
5100 794 20
ClaI AT'CG_AT
Cuts at: 1554 2904 1554
Size: 1350 4564
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 2442 3218 2442
Size: 776 5138
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4921 809
Size: 507 3605 1802
Fragments arranged by size:
3605 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3361 4250 5879
Size: 54 1425 50 275 18 1531 889 1629
Cuts at: 5879 8
Size: 43
Fragments arranged by size:
1629 1531 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 1537
Size: 275 1549 4090
Fragments arranged by size:
4090 1549 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3844 1353
Size: 2491 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2643 4501 4875 5021 387
Size: 1064 138 1054 1858 374 146 1280
Fragments arranged by size:
1858 1280 1064 1054 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3977 4574 5361 5629 1239
Size: 393 2345 597 787 268 1524
Fragments arranged by size:
2345 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2455 2693 2455
Size: 238 5676
EcoRI G'AATT_C
Cuts at: 3223 3601 3223
Size: 378 5536
EcoRV GAT'ATC
Cuts at: 2051 2761 2051
Size: 710 5204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 5914
FspI TGC'GCA
Cuts at: 4420 4420
Size: 5914
HaeII r_GCGC'y
Cuts at: 1678 2524 5061 1678
Size: 846 2537 2531
Fragments arranged by size:
2537 2531 846
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2194 2226 3050 3082 4555
Size: 32 135 32 622 32 824 32 1473
Cuts at: 4555 4587 4629 4661 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1473 824 622 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3504 669
Size: 988 281 1320 246 3079
Fragments arranged by size:
3079 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5914
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5028 5212 1305
Size: 3723 184 2007
Fragments arranged by size:
3723 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4092
Fragments arranged by size:
4092 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3872 4231 4390 518
Size: 648 2706 359 159 2042
Fragments arranged by size:
2706 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3330 4007 5155 5400 1606
Size: 1724 677 1148 245 2120
Fragments arranged by size:
2120 1724 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5362
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5914
NheI G'CTAG_C
Cuts at: 1052 3080 1052
Size: 2028 3886
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 3272 4817 1132
Size: 97 2043 1545 2229
Fragments arranged by size:
2229 2043 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5914
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5914
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2693 2693
Size: 5914
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 5882
PstI C_TGCA'G
Cuts at: 830 2122 3266 830
Size: 1292 1144 3478
Fragments arranged by size:
3478 1292 1144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 2955 4162 5874 2955
Size: 1207 1712 2995
Fragments arranged by size:
2995 1712 1207
SfcI C'TryA_G
Cuts at: 826 1045 2118 3262 4397 5078 5269 826
Size: 219 1073 1144 1135 681 191 1471
Fragments arranged by size:
1471 1144 1135 1073 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4018 4919 5181 820
Size: 197 926 2075 901 262 1553
Fragments arranged by size:
2075 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 3272 1132
Size: 2140 3774
SspI AAT'ATT
Cuts at: 5 52 2152 3838 5
Size: 47 2100 1686 2081
Fragments arranged by size:
2100 2081 1686 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5914
StyI C'CwwG_G
Cuts at: 513 1065 3353 513
Size: 552 2288 3074
Fragments arranged by size:
3074 2288 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3941 3958 4111 4296 4741 325
Size: 1255 2361 17 153 185 445 1498
Fragments arranged by size:
2361 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2721 2953 3039
Size: 274 80 33 51 1443 744 232 86
Cuts at: 3039 4160 5872 96
Size: 1121 1712 138
Fragments arranged by size:
1712 1443 1121 744 274 232 138 86
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5874 5906 5874
Size: 32 5882
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5914
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 2610 4043 2610
Size: 1433 4481
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3722 276
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI