Restriction map for
pFN21AB6575
(Circular) (Six-base) MAPSORT of: pFN21AB6575.seq Check: 5726 from: 1 to: 5833
pFN21AB6575 5833 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2450 2450
Size: 5833
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2514 3176 1937
Size: 577 662 4594
Fragments arranged by size:
4594 662 577
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 3896 5280 5548 1632
Size: 896 1368 1384 268 1917
Fragments arranged by size:
1917 1384 1368 896 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AlwNI CAG_nnn'CTG
Cuts at: 3576 5148 3576
Size: 1572 4261
ApaLI G'TGCA_C
Cuts at: 2379 3890 5046 2379
Size: 1511 1156 3166
Fragments arranged by size:
3166 1511 1156
ApoI r'AATT_y
Cuts at: 1493 2370 3142 3368 3520 5629 1493
Size: 877 772 226 152 2109 1697
Fragments arranged by size:
2109 1697 877 772 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3039 3072 3039
Size: 33 5800
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3154 4609 618
Size: 325 217 627 1367 1455 1842
Fragments arranged by size:
1842 1455 1367 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3152 1391
Size: 1761 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2021 928
Size: 971 122 4740
Fragments arranged by size:
4740 971 122
BbvCI CC'TCA_GC
Cuts at: 2109 2109
Size: 5833
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4024 4058 1903
Size: 34 50 34 2003 34 3678
Fragments arranged by size:
3678 2003 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 2811 1548
Size: 1263 4570
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 2141 3250 2141
Size: 1109 4724
Bme1580I G_kGCm'C
Cuts at: 1790 2352 2383 3894 5050 1790
Size: 562 31 1511 1156 2573
Fragments arranged by size:
2573 1511 1156 562 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2225 2225
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2049 4493 1239
Size: 810 2444 2579
Fragments arranged by size:
2579 2444 810
Bpu10I CC'TnA_GC
Cuts at: 2109 2109
Size: 5833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2297 3112 3958 4823 5121 2297
Size: 815 846 865 298 3009
Fragments arranged by size:
3009 865 846 815 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3108 4496 882
Size: 617 1609 1388 2219
Fragments arranged by size:
2219 1609 1388 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 2880 5827 2880
Size: 2947 2886
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3640 4022 4657 275
Size: 53 83 186 936 2107 382 635 1451
Fragments arranged by size:
2107 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5608
Fragments arranged by size:
5608 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2466 1186
Size: 1280 4553
BseYI C'CCAG_C
Cuts at: 2802 2993 5036 5532 2802
Size: 191 2043 496 3103
Fragments arranged by size:
3103 2043 496 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2450 1163
Size: 573 714 4546
Fragments arranged by size:
4546 714 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2383 3098 3894 3979 5050 2383
Size: 715 796 85 1071 3166
Fragments arranged by size:
3166 1071 796 715 85
BsmI GAATG_Cn'
Cuts at: 2532 3011 3344 3437 2532
Size: 479 333 93 4928
Fragments arranged by size:
4928 479 333 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2352 2383 3098 3152 3894 3979 5050
Size: 399 562 31 715 54 742 85 1071
Cuts at: 5050 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 715 562 399 85 54
31
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 2752 3722 5452 2752
Size: 970 1730 3133
Fragments arranged by size:
3133 1730 970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2450 3188 844
Size: 1606 738 3489
Fragments arranged by size:
3489 1606 738
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3071 4328 4502 65
Size: 1183 1823 1257 174 1396
Fragments arranged by size:
1823 1396 1257 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 2258 2982 96
Size: 2162 724 2947
Fragments arranged by size:
2947 2162 724
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3111 3893 4905 3111
Size: 782 1012 4039
Fragments arranged by size:
4039 1012 782
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5833
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828
Size: 1191 367 385 17 1452 86 12 346
Cuts at: 5828 1972
Size: 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3033 3033
Size: 5833
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5281
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DraIII CAC_nnn'GTG
Cuts at: 2387 2387
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3280 4169 5798
Size: 54 1425 50 275 18 1450 889 1629
Cuts at: 5798 8
Size: 43
Fragments arranged by size:
1629 1450 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2194 2778 3763 1353
Size: 841 584 985 3423
Fragments arranged by size:
3423 985 841 584
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2528 3896 4493 5280 5548 1239
Size: 393 417 479 1368 597 787 268 1524
Fragments arranged by size:
1524 1368 787 597 479 417 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2904 2904
Size: 5833
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
EcoRV GAT'ATC
Cuts at: 2160 2160
Size: 5833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2693 2693
Size: 5833
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 4980 1678
Size: 3302 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2753 2785 4474 4506 4548
Size: 32 135 32 1181 32 1689 32 42
Cuts at: 4548 4580 1373
Size: 32 2626
Fragments arranged by size:
2626 1689 1181 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2586 748
Size: 1838 3995
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2371 4947 5131 1305
Size: 1066 2576 184 2007
Fragments arranged by size:
2576 2007 1066 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2404 3791 4150 4309 518
Size: 648 1238 1387 359 159 2042
Fragments arranged by size:
2042 1387 1238 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2059 2069 3249 3926 5074 5319 1606
Size: 432 21 10 1180 677 1148 245 2120
Fragments arranged by size:
2120 1180 1148 677 432 245 21 10
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5281
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2927 3069 3191 4736 1132
Size: 97 1698 142 122 1545 2229
Fragments arranged by size:
2229 1698 1545 142 122 97
PasI CC'CwG_GG
Cuts at: 1348 2293 1348
Size: 945 4888
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2390 1108
Size: 1282 4551
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PstI C_TGCA'G
Cuts at: 830 3185 830
Size: 2355 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 3015 4081 5793 3015
Size: 1066 1712 3055
Fragments arranged by size:
3055 1712 1066
SexAI A'CCwGG_T
Cuts at: 2383 2383
Size: 5833
SfcI C'TryA_G
Cuts at: 826 1045 3181 4316 4997 5188 826
Size: 219 2136 1135 681 191 1471
Fragments arranged by size:
2136 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 3091 3937 4838 5100 820
Size: 197 926 333 815 846 901 262 1553
Fragments arranged by size:
1553 926 901 846 815 333 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 2716 152
Size: 2564 3269
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 2614 3757 5
Size: 47 2562 1143 2081
Fragments arranged by size:
2562 2081 1143 47
StuI AGG'CCT
Cuts at: 1517 2888 1517
Size: 1371 4462
StyI C'CwwG_G
Cuts at: 513 1065 3272 513
Size: 552 2207 3074
Fragments arranged by size:
3074 2207 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2973 3007 3860 3877 4030 4215 4660
Size: 1255 1393 34 853 17 153 185 445
Cuts at: 4660 325
Size: 1498
Fragments arranged by size:
1498 1393 1255 853 445 185 153 34
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2258 2982 3013
Size: 274 80 33 51 1443 281 724 31
Cuts at: 3013 4079 5791 96
Size: 1066 1712 138
Fragments arranged by size:
1712 1443 1066 724 281 274 138 80
51 33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5793 5825 5793
Size: 32 5801
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 2018 3962 2018
Size: 1944 3889
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI