Restriction map for
pFN21AB6574
(Circular) (Six-base) MAPSORT of: pFN21AB6574.seq Check: 9175 from: 1 to: 5908
pFN21AB6574 5908 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2468 3718 278
Size: 53 83 186 936 932 1250 2468
Fragments arranged by size:
2468 1250 936 932 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2676 3251 1937
Size: 739 575 4594
Fragments arranged by size:
4594 739 575
Acc65I G'GTAC_C
Cuts at: 2144 3229 2144
Size: 1085 4823
AclI AA'CG_TT
Cuts at: 1563 2072 4035 4408 1563
Size: 509 1963 373 3063
Fragments arranged by size:
3063 1963 509 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2608 2786 3002 3971 5355 5623 1632
Size: 976 178 216 969 1384 268 1917
Fragments arranged by size:
1917 1384 976 969 268 216 178
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5711
AflIII A'CryG_T
Cuts at: 1203 4807 5784 1203
Size: 3604 977 1327
Fragments arranged by size:
3604 1327 977
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 2232 1166
Size: 1066 4842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2288 1184
Size: 1104 4804
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2739 2771 2739
Size: 32 5876
AlwNI CAG_nnn'CTG
Cuts at: 2764 2980 3651 5223 2764
Size: 216 671 1572 3449
Fragments arranged by size:
3449 1572 671 216
ApaLI G'TGCA_C
Cuts at: 3965 5121 3965
Size: 1156 4752
ApoI r'AATT_y
Cuts at: 1493 3217 3443 3595 5704 1493
Size: 1724 226 152 2109 1697
Fragments arranged by size:
2109 1724 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4462 160
Size: 872 3430 1606
Fragments arranged by size:
3430 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 3233 1870
Size: 73 1290 4545
Fragments arranged by size:
4545 1290 73
BamHI G'GATC_C
Cuts at: 3238 3605 3238
Size: 367 5541
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2690 3229 4684 618
Size: 325 217 627 357 546 539 1455 1842
Fragments arranged by size:
1842 1455 627 546 539 357 325 217
BanII G_rGCy'C
Cuts at: 1391 3227 1391
Size: 1836 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2186 2940 3110 928
Size: 971 287 754 170 3726
Fragments arranged by size:
3726 971 754 287 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4099 4133 1903
Size: 34 2162 34 3678
Fragments arranged by size:
3678 2162 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2659 4519 136
Size: 107 122 71 631 605 987 1860 1525
Fragments arranged by size:
1860 1525 987 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5903 5903
Size: 5908
BlpI GC'TnA_GC
Cuts at: 3325 3325
Size: 5908
Bme1580I G_kGCm'C
Cuts at: 1790 3969 5125 1790
Size: 2179 1156 2573
Fragments arranged by size:
2573 2179 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2961 3639 4597
Size: 416 53 83 399 24 1544 678 958
Cuts at: 4597 442
Size: 1753
Fragments arranged by size:
1753 1544 958 678 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5908
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2394 2394
Size: 5908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4568 1239
Size: 3329 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2363 4033 4898 5196 2363
Size: 1670 865 298 3075
Fragments arranged by size:
3075 1670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2519 4571 882
Size: 617 1020 2052 2219
Fragments arranged by size:
2219 2052 1020 617
BsaAI yAC'GTr
Cuts at: 493 1204 3699 493
Size: 711 2495 2702
Fragments arranged by size:
2702 2495 711
BsaBI GATnn'nnATC
Cuts at: 5902 5902
Size: 5908
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2465 3715 4097 4732
Size: 53 83 186 936 932 1250 382 635
Cuts at: 4732 275
Size: 1451
Fragments arranged by size:
1451 1250 936 932 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2561 4341 4739 5013 5160 5571 2561
Size: 1780 398 274 147 411 2898
Fragments arranged by size:
2898 1780 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2062 2092 2261 2291 1178
Size: 30 165 30 659 30 169 30 4795
Fragments arranged by size:
4795 659 169 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2627 1186
Size: 1441 4467
BseYI C'CCAG_C
Cuts at: 5111 5607 5111
Size: 496 5412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2867 1163
Size: 573 1131 4204
Fragments arranged by size:
4204 1131 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3358 4119 5147 1540
Size: 275 186 1357 761 1028 2301
Fragments arranged by size:
2301 1357 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3969 4054 5125 3969
Size: 85 1071 4752
Fragments arranged by size:
4752 1071 85
BsmI GAATG_Cn'
Cuts at: 2327 2618 3419 3512 2327
Size: 291 801 93 4723
Fragments arranged by size:
4723 801 291 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2456 2456
Size: 5908
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3227 3969 4054 5125 1391
Size: 399 1437 742 85 1071 2174
Fragments arranged by size:
2174 1437 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2561 5571 2561
Size: 3010 2898
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3263 844
Size: 2419 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3795 1394
Size: 429 1972 3507
Fragments arranged by size:
3507 1972 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4403 4577 65
Size: 1183 3155 174 1396
Fragments arranged by size:
3155 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4552 4739 1700
Size: 110 2742 187 2869
Fragments arranged by size:
2869 2742 187 110
BsrGI T'GTAC_A
Cuts at: 96 2165 3135 96
Size: 2069 970 2869
Fragments arranged by size:
2869 2069 970
BssHII G'CGCG_C
Cuts at: 1925 2025 1925
Size: 100 5808
BssSI C'ACGA_G
Cuts at: 2030 3968 4980 2030
Size: 1938 1012 2958
Fragments arranged by size:
2958 1938 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5908
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 3173 1184
Size: 1989 3919
BstYI r'GATC_y
Cuts at: 1972 2976 3238 3605 3990 4007 5459 5545 5557
Size: 1004 262 367 385 17 1452 86 12
Cuts at: 5557 5903 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1004 385 367 346 262 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5356
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5169
Fragments arranged by size:
5169 683 56
BtsI GCAGTG_nn'
Cuts at: 2509 3424 4218 4238 2509
Size: 915 794 20 4179
Fragments arranged by size:
4179 915 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4915 809
Size: 507 3599 1802
Fragments arranged by size:
3599 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3355 4244 5873
Size: 54 1425 50 275 18 1525 889 1629
Cuts at: 5873 8
Size: 43
Fragments arranged by size:
1629 1525 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3355 1537
Size: 275 1543 4090
Fragments arranged by size:
4090 1543 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3838 1353
Size: 2485 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4495 4869 5015 387
Size: 1064 138 2906 374 146 1280
Fragments arranged by size:
2906 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2608 2786 3002 3971 4568 5355 5623
Size: 393 976 178 216 969 597 787 268
Cuts at: 5623 1239
Size: 1524
Fragments arranged by size:
1524 976 969 787 597 393 268 216
178
EcoNI CCTnn'n_nnAGG
Cuts at: 2609 2609
Size: 5908
EcoO109I rG'GnC_Cy
Cuts at: 3034 3034
Size: 5908
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
FspI TGC'GCA
Cuts at: 4414 4414
Size: 5908
HaeII r_GCGC'y
Cuts at: 1678 2338 5055 1678
Size: 660 2717 2531
Fragments arranged by size:
2717 2531 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 4549 4581 4623
Size: 32 135 32 881 32 2064 32 42
Cuts at: 4623 4655 1373
Size: 32 2626
Fragments arranged by size:
2626 2064 881 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3252 3498 669
Size: 988 281 1314 246 3079
Fragments arranged by size:
3079 1314 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2905 748
Size: 2157 3751
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KpnI G_GTAC'C
Cuts at: 2148 3233 2148
Size: 1085 4823
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5022 5206 1305
Size: 3717 184 2007
Fragments arranged by size:
3717 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2232 3866 4225 4384 518
Size: 648 1066 1634 359 159 2042
Fragments arranged by size:
2042 1634 1066 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2223 2761 2851 3324 4001 5149 5394
Size: 513 104 538 90 473 677 1148 245
Cuts at: 5394 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 538 513 473 245 104
90
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5356
NdeI CA'TA_TG
Cuts at: 387 2555 387
Size: 2168 3740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5908
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 2867 2961 3266 4811 1132
Size: 97 1638 94 305 1545 2229
Fragments arranged by size:
2229 1638 1545 305 97 94
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5908
PciI A'CATG_T
Cuts at: 4807 4807
Size: 5908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2055 1108
Size: 947 4961
PfoI T'CCnGG_A
Cuts at: 2380 2380
Size: 5908
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2336 2368 3977 4009 5490 5522 2336
Size: 32 1609 32 1481 32 2722
Fragments arranged by size:
2722 1609 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2133 2241 1613
Size: 520 108 5280
Fragments arranged by size:
5280 520 108
PsiI TTA'TAA
Cuts at: 3478 3478
Size: 5908
PstI C_TGCA'G
Cuts at: 830 2255 2277 3260 830
Size: 1425 22 983 3478
Fragments arranged by size:
3478 1425 983 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5908
PvuII CAG'CTG
Cuts at: 2119 2223 2761 2119
Size: 104 538 5266
Fragments arranged by size:
5266 538 104
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 4156 5868 4156
Size: 1712 4196
SexAI A'CCwGG_T
Cuts at: 3177 3177
Size: 5908
SfcI C'TryA_G
Cuts at: 826 1045 2251 2273 2482 3256 4391 5072 5263
Size: 219 1206 22 209 774 1135 681 191
Cuts at: 5263 826
Size: 1471
Fragments arranged by size:
1471 1206 1135 774 681 219 209 191
22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5908
SmaI CCC'GGG
Cuts at: 1872 3235 1872
Size: 1363 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 4012 4913 5175 820
Size: 197 926 399 1670 901 262 1553
Fragments arranged by size:
1670 1553 926 901 399 262 197
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 2961 3266 1132
Size: 1829 305 3774
Fragments arranged by size:
3774 1829 305
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5908
StyI C'CwwG_G
Cuts at: 513 1065 3347 513
Size: 552 2282 3074
Fragments arranged by size:
3074 2282 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 2318 3935 3952 4105 4290 4735
Size: 1255 498 240 1617 17 153 185 445
Cuts at: 4735 325
Size: 1498
Fragments arranged by size:
1617 1498 1255 498 445 240 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2165 3135 4154
Size: 274 80 33 51 1443 188 970 1019
Cuts at: 4154 5866 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1019 970 274 188 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2376 2408 2907 2939 5868 5900 2376
Size: 32 499 32 2929 32 2384
Fragments arranged by size:
2929 2384 499 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5908
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5908
XmaI C'CCGG_G
Cuts at: 1870 3233 1870
Size: 1363 4545
XmnI GAAnn'nnTTC
Cuts at: 2073 4037 2073
Size: 1964 3944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2466 3716 276
Size: 53 83 186 936 932 1250 2468
Fragments arranged by size:
2468 1250 936 932 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI