Restriction map for
pFN21AB6571
(Circular) (Six-base) MAPSORT of: pFN21AB6571.seq Check: 2190 from: 1 to: 5830
pFN21AB6571 5830 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2561 3111 3173 1937
Size: 624 550 62 4594
Fragments arranged by size:
4594 624 550 62
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3893 5277 5545 1632
Size: 2261 1384 268 1917
Fragments arranged by size:
2261 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3034 1184
Size: 1850 3980
AlwNI CAG_nnn'CTG
Cuts at: 2378 2603 3573 5145 2378
Size: 225 970 1572 3063
Fragments arranged by size:
3063 1572 970 225
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 2426 2945 3093 3139 3365 3517 5626 1493
Size: 933 519 148 46 226 152 2109 1697
Fragments arranged by size:
2109 1697 933 519 226 152 148 46
AseI AT'TA_AT
Cuts at: 160 1032 3048 4384 160
Size: 872 2016 1336 1606
Fragments arranged by size:
2016 1606 1336 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3155 1870
Size: 73 1212 4545
Fragments arranged by size:
4545 1212 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3151 4606 618
Size: 325 217 627 1364 1455 1842
Fragments arranged by size:
1842 1455 1364 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3149 1391
Size: 1758 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2636 928
Size: 971 737 4122
Fragments arranged by size:
4122 971 737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4021 4055 1903
Size: 34 50 34 2000 34 3678
Fragments arranged by size:
3678 2000 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 2278 1548
Size: 730 5100
BfrBI ATG'CAT
Cuts at: 2126 2126
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4441 136
Size: 107 122 71 631 605 2769 1525
Fragments arranged by size:
2769 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 3891 5047 1790
Size: 2101 1156 2573
Fragments arranged by size:
2573 2101 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3561 4519 442
Size: 416 53 83 399 24 2144 958 1753
Fragments arranged by size:
2144 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2955 4490 1239
Size: 1716 1535 2579
Fragments arranged by size:
2579 1716 1535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 4820 5118 3955
Size: 865 298 4667
Fragments arranged by size:
4667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2479 4493 882
Size: 617 980 2014 2219
Fragments arranged by size:
2219 2014 980 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 2180 5824 2180
Size: 3644 2186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4019 4654 275
Size: 53 83 186 936 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5605
Fragments arranged by size:
5605 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2247 1186
Size: 1061 4769
BseYI C'CCAG_C
Cuts at: 5033 5529 5033
Size: 496 5334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2829 3128 3891 3976 5047 2829
Size: 299 763 85 1071 3612
Fragments arranged by size:
3612 1071 763 299 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2829 3128 3149 3891 3976 5047 1391
Size: 399 1039 299 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 1039 742 399 299 85 21
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 2707 3719 5449 2707
Size: 1012 1730 3088
Fragments arranged by size:
3088 1730 1012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3185 844
Size: 2341 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4474 4661 1700
Size: 110 2664 187 2869
Fragments arranged by size:
2869 2664 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 3890 4902 3890
Size: 1012 4818
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2014 794
Size: 1220 4610
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 2068 2792 3160 3527 3912 3929 5381 5467
Size: 96 724 368 367 385 17 1452 86
Cuts at: 5467 5479 5825 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 724 385 368 367 346 96
86 17 12
BstZ17I GTA'TAC
Cuts at: 2562 2562
Size: 5830
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5278
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2325 3116 487
Size: 683 56 1099 791 3201
Fragments arranged by size:
3201 1099 791 683 56
BtsI GCAGTG_nn'
Cuts at: 3346 4140 4160 3346
Size: 794 20 5016
Fragments arranged by size:
5016 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 2425 3134 2425
Size: 709 5121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3277 4166 5795
Size: 54 1425 50 275 18 1447 889 1629
Cuts at: 5795 8
Size: 43
Fragments arranged by size:
1629 1447 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3760 1353
Size: 2407 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2955 3893 4490 5277 5545 1239
Size: 393 1323 938 597 787 268 1524
Fragments arranged by size:
1524 1323 938 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3014 3014
Size: 5830
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 4977 1678
Size: 3299 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2813 2845 2887 2919 4471
Size: 32 135 32 1241 32 42 32 1552
Cuts at: 4471 4503 4545 4577 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1552 1241 135 42 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2688 2877 748
Size: 1940 189 3701
Fragments arranged by size:
3701 1940 189
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2691 4944 5128 1305
Size: 1386 2253 184 2007
Fragments arranged by size:
2253 2007 1386 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4008
Fragments arranged by size:
4008 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2624 3035 3788 4147 4306 518
Size: 648 1197 261 411 753 359 159 2042
Fragments arranged by size:
2042 1197 753 648 411 359 261 159
MspA1I CmG'CkG
Cuts at: 1606 3117 3246 3923 5071 5316 1606
Size: 1511 129 677 1148 245 2120
Fragments arranged by size:
2120 1511 1148 677 245 129
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5278
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NsiI A_TGCA'T
Cuts at: 2128 2128
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2130 3188 4733 1132
Size: 97 901 1058 1545 2229
Fragments arranged by size:
2229 1545 1058 901 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2603 1108
Size: 1495 4335
PfoI T'CCnGG_A
Cuts at: 2265 2265
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 2234 3400 2234
Size: 1166 4664
PstI C_TGCA'G
Cuts at: 830 3182 830
Size: 2352 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2370 3178 4313 4994 5185 826
Size: 219 1325 808 1135 681 191 1471
Fragments arranged by size:
1471 1325 1135 808 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3934 4835 5097 820
Size: 197 926 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 3269 513
Size: 552 2204 3074
Fragments arranged by size:
3074 2204 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3111 3857 3874 4027 4212 4657 325
Size: 1255 1531 746 17 153 185 445 1498
Fragments arranged by size:
1531 1498 1255 746 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2129 3023 4076
Size: 274 80 33 51 1443 152 894 1053
Cuts at: 4076 5788 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1053 894 274 152 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5790 5822 5790
Size: 32 5798
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI