Restriction map for
pFN21AB6569
(Circular) (Six-base) MAPSORT of: pFN21AB6569.seq Check: 6873 from: 1 to: 5830
pFN21AB6569 5830 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3893 5277 5545 1632
Size: 2261 1384 268 1917
Fragments arranged by size:
2261 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AlwNI CAG_nnn'CTG
Cuts at: 2050 2216 2558 2842 3573 5145 2050
Size: 166 342 284 731 1572 2735
Fragments arranged by size:
2735 1572 731 342 284 166
ApaI G_GGCC'C
Cuts at: 2180 2262 2433 3019 2180
Size: 82 171 586 4991
Fragments arranged by size:
4991 586 171 82
ApaLI G'TGCA_C
Cuts at: 2845 3887 5043 2845
Size: 1042 1156 3632
Fragments arranged by size:
3632 1156 1042
ApoI r'AATT_y
Cuts at: 1493 3139 3365 3517 5626 1493
Size: 1646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 2181 2605 3155 1870
Size: 73 238 424 550 4545
Fragments arranged by size:
4545 550 424 238 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2447 2591 3151 4606 618
Size: 325 217 627 660 144 560 1455 1842
Fragments arranged by size:
1842 1455 660 627 560 325 217 144
BanII G_rGCy'C
Cuts at: 1391 2180 2262 2433 2468 2763 3019 3030 3149
Size: 789 82 171 35 295 256 11 119
Cuts at: 3149 1391
Size: 4072
Fragments arranged by size:
4072 789 295 256 171 119 82 35
11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2081 928
Size: 971 182 4677
Fragments arranged by size:
4677 971 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4021 4055 1903
Size: 34 50 34 2000 34 3678
Fragments arranged by size:
3678 2000 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2184 2490 4441
Size: 107 122 71 631 605 512 306 1951
Cuts at: 4441 136
Size: 1525
Fragments arranged by size:
1951 1525 631 605 512 306 122 107
71
BglII A'GATC_T
Cuts at: 3063 5825 3063
Size: 2762 3068
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 2180 2262 2433 2452 2849 3019 3891 5047
Size: 390 82 171 19 397 170 872 1156
Cuts at: 5047 1790
Size: 2573
Fragments arranged by size:
2573 1156 872 397 390 171 170 82
19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2094 2246 2319
Size: 416 53 83 399 24 677 152 73
Cuts at: 2319 3022 3561 4519 442
Size: 703 539 958 1753
Fragments arranged by size:
1753 958 703 677 539 416 399 152
83 73 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 2583 2655 2711 4490 1239
Size: 1071 273 72 56 1779 2579
Fragments arranged by size:
2579 1779 1071 273 72 56
Bpu10I CC'TnA_GC
Cuts at: 2942 2942
Size: 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 4820 5118 3955
Size: 865 298 4667
Fragments arranged by size:
4667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4493 882
Size: 617 2994 2219
Fragments arranged by size:
2994 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4019 4654 275
Size: 53 83 186 936 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2709 2739 1178
Size: 30 165 30 1306 30 4269
Fragments arranged by size:
4269 1306 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2009 1186
Size: 823 5007
BseYI C'CCAG_C
Cuts at: 2426 5033 5529 2426
Size: 2607 496 2727
Fragments arranged by size:
2727 2607 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2010 1163
Size: 573 274 4983
Fragments arranged by size:
4983 573 274
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2763 2849 3891 3976 5047 2763
Size: 86 1042 85 1071 3546
Fragments arranged by size:
3546 1071 1042 86 85
BsmI GAATG_Cn'
Cuts at: 2628 2899 3341 3434 2628
Size: 271 442 93 5024
Fragments arranged by size:
5024 442 271 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2262 2433 2452 2468 2763 2849
Size: 399 390 82 171 19 16 295 86
Cuts at: 2849 3019 3030 3149 3891 3976 5047 1391
Size: 170 11 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 295 171 170
119 86 85 82 19 16 11
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2683 3185 844
Size: 1839 502 3489
Fragments arranged by size:
3489 1839 502
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4474 4661 1700
Size: 110 2664 187 2869
Fragments arranged by size:
2869 2664 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 3890 4902 3890
Size: 1012 4818
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2558 794
Size: 1764 4066
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 3063 3160 3527 3912 3929 5381 5467 5479
Size: 1091 97 367 385 17 1452 86 12
Cuts at: 5479 5825 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1091 385 367 346 97 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2397 2496 2397
Size: 99 5731
BtgI C'CryG_G
Cuts at: 513 1065 2568 513
Size: 552 1503 3775
Fragments arranged by size:
3775 1503 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2169 487
Size: 683 56 943 4148
Fragments arranged by size:
4148 943 683 56
BtsI GCAGTG_nn'
Cuts at: 3346 4140 4160 3346
Size: 794 20 5016
Fragments arranged by size:
5016 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2773 3277 4166
Size: 54 1425 50 275 18 943 504 889
Cuts at: 4166 5795 8
Size: 1629 43
Fragments arranged by size:
1629 1425 943 889 504 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2353 2768 3760 1353
Size: 665 335 415 992 3423
Fragments arranged by size:
3423 992 665 415 335
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2761 2761
Size: 5830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2583 2655 2711 3893 4490 5277
Size: 393 678 273 72 56 1182 597 787
Cuts at: 5277 5545 1239
Size: 268 1524
Fragments arranged by size:
1524 1182 787 678 597 393 273 268
72 56
EcoO109I rG'GnC_Cy
Cuts at: 2176 2177 2197 2330 2366 2393 2492 2573 2600
Size: 1 20 133 36 27 99 81 27
Cuts at: 2600 3016 2176
Size: 416 4990
Fragments arranged by size:
4990 416 133 99 81 36 27 27
20 1
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 4977 1678
Size: 3299 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 3029 3061 4471
Size: 32 135 32 689 32 736 32 1410
Cuts at: 4471 4503 4545 4577 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1410 736 689 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2263 3033 4944 5128 1305
Size: 958 770 1911 184 2007
Fragments arranged by size:
2007 1911 958 770 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2775 10
Size: 54 1425 343 943 3065
Fragments arranged by size:
3065 1425 943 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3788 4147 4306 518
Size: 648 2622 359 159 2042
Fragments arranged by size:
2622 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2354 2839 2888 2911 3246 3923 5071 5316
Size: 748 485 49 23 335 677 1148 245
Cuts at: 5316 1606
Size: 2120
Fragments arranged by size:
2120 1148 748 677 485 335 245 49
23
NcoI C'CATG_G
Cuts at: 513 1065 2568 513
Size: 552 1503 3775
Fragments arranged by size:
3775 1503 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NruI TCG'CGA
Cuts at: 2154 2154
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2461 3188 4733 1132
Size: 97 1232 727 1545 2229
Fragments arranged by size:
2229 1545 1232 727 97
PasI CC'CwG_GG
Cuts at: 1348 2050 2371 2578 3020 1348
Size: 702 321 207 442 4158
Fragments arranged by size:
4158 702 442 321 207
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PfoI T'CCnGG_A
Cuts at: 2326 2632 2326
Size: 306 5524
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 3899 3931 5412 5444 2957
Size: 32 910 32 1481 32 3343
Fragments arranged by size:
3343 1481 910 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2197 2330 2366 2393 2573 2600 2197
Size: 133 36 27 180 27 5427
Fragments arranged by size:
5427 180 133 36 27 27
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspOMI G'GGCC_C
Cuts at: 2176 2258 2429 3015 2176
Size: 82 171 586 4991
Fragments arranged by size:
4991 586 171 82
PstI C_TGCA'G
Cuts at: 830 2221 3182 830
Size: 1391 961 3478
Fragments arranged by size:
3478 1391 961
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
PvuII CAG'CTG
Cuts at: 2354 2839 2911 2354
Size: 485 72 5273
Fragments arranged by size:
5273 485 72
SacI G_AGCT'C
Cuts at: 2763 2763
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SanDI GG'GwC_CC
Cuts at: 2197 2573 2600 2197
Size: 376 27 5427
Fragments arranged by size:
5427 376 27
SapI GCTCTTCn'nnn_
Cuts at: 2018 2768 2018
Size: 750 5080
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2217 3178 4313 4994 5185 826
Size: 219 1172 961 1135 681 191 1471
Fragments arranged by size:
1471 1172 1135 961 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2490 2490
Size: 5830
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 2607 3157 1872
Size: 735 550 4545
Fragments arranged by size:
4545 735 550
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3934 4835 5097 820
Size: 197 926 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 2461 3188 1132
Size: 1329 727 3774
Fragments arranged by size:
3774 1329 727
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 2484 2702 1517
Size: 967 218 4645
Fragments arranged by size:
4645 967 218
StyI C'CwwG_G
Cuts at: 513 1065 2568 3269 513
Size: 552 1503 701 3074
Fragments arranged by size:
3074 1503 701 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3857 3874 4027 4212 4657 325
Size: 1255 2277 17 153 185 445 1498
Fragments arranged by size:
2277 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2716 4076 5788
Size: 274 80 33 51 1443 739 1360 1712
Cuts at: 5788 96
Size: 138
Fragments arranged by size:
1712 1443 1360 739 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5790 5822 5790
Size: 32 5798
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 2605 3155 1870
Size: 735 550 4545
Fragments arranged by size:
4545 735 550
XmnI GAAnn'nnTTC
Cuts at: 2795 3959 2795
Size: 1164 4666
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI
SfoI SrfI SwaI XcmI