Restriction map for
pFN21AB6565
(Circular) (Six-base) MAPSORT of: pFN21AB6565.seq Check: 137 from: 1 to: 5827
pFN21AB6565 5827 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2695 2695
Size: 5827
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 2394 3148 2394
Size: 754 5073
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 2624 2786 3890 5274 5542 1632
Size: 872 120 162 1104 1384 268 1917
Fragments arranged by size:
1917 1384 1104 872 268 162 120
AfeI AGC'GCT
Cuts at: 2822 2822
Size: 5827
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 4726 5703 1203
Size: 3523 977 1327
Fragments arranged by size:
3523 1327 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 3570 5142 3570
Size: 1572 4255
ApaI G_GGCC'C
Cuts at: 2796 2796
Size: 5827
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 3136 3362 3514 5623 1493
Size: 1643 226 152 2109 1697
Fragments arranged by size:
2109 1697 1643 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 2883 3152 1870
Size: 73 194 746 269 4545
Fragments arranged by size:
4545 746 269 194 73
BamHI G'GATC_C
Cuts at: 2086 2328 3157 3524 2086
Size: 242 829 367 4389
Fragments arranged by size:
4389 829 367 242
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2394 2740 3148 4603
Size: 325 217 627 291 316 346 408 1455
Cuts at: 4603 618
Size: 1842
Fragments arranged by size:
1842 1455 627 408 346 325 316 291
217
BanII G_rGCy'C
Cuts at: 1391 2382 2517 2796 3146 1391
Size: 991 135 279 350 4072
Fragments arranged by size:
4072 991 350 279 135
BbeI G_GCGC'C
Cuts at: 2082 2082
Size: 5827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2110 2792 2887 928
Size: 971 211 682 95 3868
Fragments arranged by size:
3868 971 682 211 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2965 2999 4018 4052 1903
Size: 34 1028 34 1019 34 3678
Fragments arranged by size:
3678 1028 1019 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4438 136
Size: 107 122 71 631 605 2766 1525
Fragments arranged by size:
2766 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2734 5822 2734
Size: 3088 2739
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 2796 3888 5044 1790
Size: 1006 1092 1156 2573
Fragments arranged by size:
2573 1156 1092 1006
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2104 3558 4516
Size: 416 53 83 399 24 687 1454 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1454 958 687 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2737 4487 1239
Size: 815 683 1750 2579
Fragments arranged by size:
2579 1750 815 683
Bpu10I CC'TnA_GC
Cuts at: 2129 2129
Size: 5827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4490 882
Size: 617 2991 2219
Fragments arranged by size:
2991 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3618 493
Size: 711 2414 2702
Fragments arranged by size:
2702 2414 711
BsaBI GATnn'nnATC
Cuts at: 2531 5821 2531
Size: 3290 2537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 2115 2193 2241
Size: 53 83 186 936 546 36 78 48
Cuts at: 2241 2874 3634 4016 4651 275
Size: 633 760 382 635 1451
Fragments arranged by size:
1451 936 760 635 633 546 382 186
83 78 53 48 36
BsaWI w'CCGG_w
Cuts at: 2148 2814 4260 4658 4932 5079 5490 2148
Size: 666 1446 398 274 147 411 2485
Fragments arranged by size:
2485 1446 666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 1178
Size: 30 165 30 681 30 4891
Fragments arranged by size:
4891 681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 1186
Size: 1030 4797
BseYI C'CCAG_C
Cuts at: 5030 5526 5030
Size: 496 5331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5254
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2051 2998 3277 4038 5066 1540
Size: 275 186 50 947 279 761 1028 2301
Fragments arranged by size:
2301 1028 947 761 279 275 186 50
BsiHKAI G_wGCw'C
Cuts at: 2482 2541 3888 3973 5044 2482
Size: 59 1347 85 1071 3265
Fragments arranged by size:
3265 1347 1071 85 59
BsmI GAATG_Cn'
Cuts at: 2711 3338 3431 2711
Size: 627 93 5107
Fragments arranged by size:
5107 627 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3014 3014
Size: 5827
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 2482 2517 2541 2796 3146 3888
Size: 399 592 100 35 24 255 350 742
Cuts at: 3888 3973 5044 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 592 399 350 255 100
85 35 24
BspEI T'CCGG_A
Cuts at: 2814 5490 2814
Size: 2676 3151
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2581 2695 2709 3182 844
Size: 1737 114 14 473 3489
Fragments arranged by size:
3489 1737 473 114 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 3714 1394
Size: 429 194 1697 3507
Fragments arranged by size:
3507 1697 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2286 2560 4471 4658 1700
Size: 110 476 274 1911 187 2869
Fragments arranged by size:
2869 1911 476 274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 2060 1925
Size: 135 5692
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 2593 1184
Size: 1409 4418
BstYI r'GATC_y
Cuts at: 1972 2086 2328 2734 3157 3524 3909 3926 5378
Size: 114 242 406 423 367 385 17 1452
Cuts at: 5378 5464 5476 5822 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 423 406 385 367 346 242
114 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2230 2581 2832 513
Size: 552 939 226 351 251 3508
Fragments arranged by size:
3508 939 552 351 251 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 2330 3105 3343 4137 4157 2330
Size: 775 238 794 20 4000
Fragments arranged by size:
4000 794 775 238 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2896 2931 529
Size: 35 2332 35 3425
Fragments arranged by size:
3425 2332 35 35
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2048 2422
Size: 54 1425 50 275 18 177 41 374
Cuts at: 2422 2562 2572 2966 3274 4163 5792 8
Size: 140 10 394 308 889 1629 43
Fragments arranged by size:
1629 1425 889 394 374 308 275 177
140 54 50 43 41 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2048 3274 1537
Size: 275 236 1226 4090
Fragments arranged by size:
4090 1226 275 236
EarI CTCTTCn'nnn_
Cuts at: 1353 3757 1353
Size: 2404 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 4414 4788 4934 387
Size: 1064 138 585 2240 374 146 1280
Fragments arranged by size:
2240 1280 1064 585 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2504 2624 2737 2786 3890 4487
Size: 393 422 450 120 113 49 1104 597
Cuts at: 4487 5274 5542 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1104 787 597 450 422 393 268
120 113 49
EcoO109I rG'GnC_Cy
Cuts at: 2792 2792
Size: 5827
EcoRI G'AATT_C
Cuts at: 3136 3514 3136
Size: 378 5449
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 2082 2824 4974 1678
Size: 404 742 2150 2531
Fragments arranged by size:
2531 2150 742 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2877 2909 3011
Size: 32 135 32 512 32 761 32 102
Cuts at: 3011 3043 4468 4500 4542 4574 1373
Size: 32 1425 32 42 32 2626
Fragments arranged by size:
2626 1425 761 512 135 102 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3171 3417 669
Size: 988 281 1233 246 3079
Fragments arranged by size:
3079 1233 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KasI G'GCGC_C
Cuts at: 2078 2078
Size: 5827
KpnI G_GTAC'C
Cuts at: 2398 3152 2398
Size: 754 5073
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2325 3054 4941 5125 1305
Size: 1020 729 1887 184 2007
Fragments arranged by size:
2007 1887 1020 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2968 10
Size: 54 1425 343 177 959 2869
Fragments arranged by size:
2869 1425 959 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3785 4144 4303 518
Size: 648 2619 359 159 2042
Fragments arranged by size:
2619 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 3243 3920 5068 5313 1606
Size: 441 1196 677 1148 245 2120
Fragments arranged by size:
2120 1196 1148 677 441 245
NaeI GCC'GGC
Cuts at: 2562 2562
Size: 5827
NarI GG'CG_CC
Cuts at: 2079 2079
Size: 5827
NcoI C'CATG_G
Cuts at: 513 1065 2004 2832 513
Size: 552 939 828 3508
Fragments arranged by size:
3508 939 828 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NgoMIV G'CCGG_C
Cuts at: 2560 2560
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 3185 4730 1132
Size: 97 1956 1545 2229
Fragments arranged by size:
2229 1956 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2717 2953 1348
Size: 1369 236 4222
Fragments arranged by size:
4222 1369 236
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5827
PfoI T'CCnGG_A
Cuts at: 2031 2753 2031
Size: 722 5105
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspOMI G'GGCC_C
Cuts at: 2792 2792
Size: 5827
PstI C_TGCA'G
Cuts at: 830 2046 2592 2706 3179 830
Size: 1216 546 114 473 3478
Fragments arranged by size:
3478 1216 546 473 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SbfI CC_TGCA'GG
Cuts at: 2592 2706 3179 2592
Size: 114 473 5240
Fragments arranged by size:
5240 473 114
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SfcI C'TryA_G
Cuts at: 826 1045 2042 2588 2702 3175 4310 4991 5182
Size: 219 997 546 114 473 1135 681 191
Cuts at: 5182 826
Size: 1471
Fragments arranged by size:
1471 1135 997 681 546 473 219 191
114
SfoI GGC'GCC
Cuts at: 2080 2080
Size: 5827
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3931 4832 5094 820
Size: 197 926 1988 901 262 1553
Fragments arranged by size:
1988 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5827
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2508 2517 2832 3266 513
Size: 552 939 6 498 9 315 434 3074
Fragments arranged by size:
3074 939 552 498 434 315 9 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2966 3034 3854 3871 4024 4209 4654
Size: 1255 1386 68 820 17 153 185 445
Cuts at: 4654 325
Size: 1498
Fragments arranged by size:
1498 1386 1255 820 445 185 153 68
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2853 4073 5785
Size: 274 80 33 51 1443 876 1220 1712
Cuts at: 5785 96
Size: 138
Fragments arranged by size:
1712 1443 1220 876 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5787 5819 5787
Size: 32 5795
Tth111I GACn'n_nGTC
Cuts at: 1316 3025 1316
Size: 1709 4118
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2969 2969
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 2489 3956 2489
Size: 1467 4360
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI