Restriction map for
pFN21AB6564
(Circular) (Six-base) MAPSORT of: pFN21AB6564.seq Check: 8361 from: 1 to: 5905
pFN21AB6564 5905 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2856 3248 1937
Size: 919 392 4594
Fragments arranged by size:
4594 919 392
Acc65I G'GTAC_C
Cuts at: 2796 3226 2796
Size: 430 5475
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3104 3968 5352 5620 1632
Size: 1472 864 1384 268 1917
Fragments arranged by size:
1917 1472 1384 864 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 3200 1166
Size: 2034 3871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 2368 2479 3648 5220 2368
Size: 111 1169 1572 3053
Fragments arranged by size:
3053 1572 1169 111
ApaI G_GGCC'C
Cuts at: 2069 2069
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2284 2501 2906 3230 1870
Size: 73 341 217 405 324 4545
Fragments arranged by size:
4545 405 341 324 217 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2796 2910 3226 4681
Size: 325 217 627 300 709 114 316 1455
Cuts at: 4681 618
Size: 1842
Fragments arranged by size:
1842 1455 709 627 325 316 300 217
114
BanII G_rGCy'C
Cuts at: 1391 2069 3224 1391
Size: 678 1155 4072
Fragments arranged by size:
4072 1155 678
BbeI G_GCGC'C
Cuts at: 2091 2914 2091
Size: 823 5082
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4096 4130 1903
Size: 34 50 34 2075 34 3678
Fragments arranged by size:
3678 2075 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2096 2908
Size: 107 122 71 631 605 337 87 812
Cuts at: 2908 4516 136
Size: 1608 1525
Fragments arranged by size:
1608 1525 812 631 605 337 122 107
87 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2069 2693 2729 3966 5122 1790
Size: 279 624 36 1237 1156 2573
Fragments arranged by size:
2573 1237 1156 624 279 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3636 4594 442
Size: 416 53 83 399 24 2219 958 1753
Fragments arranged by size:
2219 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2776 2849 4565 1239
Size: 908 629 73 1716 2579
Fragments arranged by size:
2579 1716 908 629 73
Bpu10I CC'TnA_GC
Cuts at: 2997 2997
Size: 5905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 4030 4895 5193 2339
Size: 1691 865 298 3051
Fragments arranged by size:
3051 1691 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2817 2911 3712
Size: 53 83 186 936 555 729 94 801
Cuts at: 3712 4094 4729 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 801 729 635 555 382 186
94 83 53
BsaWI w'CCGG_w
Cuts at: 2337 2710 4338 4736 5010 5157 5568 2337
Size: 373 1628 398 274 147 411 2674
Fragments arranged by size:
2674 1628 411 398 373 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2447 2477 1178
Size: 30 165 30 1044 30 4606
Fragments arranged by size:
4606 1044 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2519 1186
Size: 958 375 4572
Fragments arranged by size:
4572 958 375
BseYI C'CCAG_C
Cuts at: 2236 3064 5108 5604 2236
Size: 828 2044 496 2537
Fragments arranged by size:
2537 2044 828 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 2762 1163
Size: 573 618 408 4306
Fragments arranged by size:
4306 618 573 408
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2897 3355 4116 5144 1540
Size: 275 186 896 458 761 1028 2301
Fragments arranged by size:
2301 1028 896 761 458 275 186
BsiHKAI G_wGCw'C
Cuts at: 3966 4051 5122 3966
Size: 85 1071 4749
Fragments arranged by size:
4749 1071 85
BsmI GAATG_Cn'
Cuts at: 2381 3416 3509 2381
Size: 1035 93 4777
Fragments arranged by size:
4777 1035 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2693 2729 3224 3966 4051 5122
Size: 399 279 624 36 495 742 85 1071
Cuts at: 5122 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 624 495 399 279 85
36
BspEI T'CCGG_A
Cuts at: 2337 2710 5568 2337
Size: 373 2858 2674
Fragments arranged by size:
2858 2674 373
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 2353 3260 844
Size: 1277 232 907 3489
Fragments arranged by size:
3489 1277 907 232
BsrBI CCG'CTC
Cuts at: 1394 1823 2652 2716 3073 3792 1394
Size: 429 829 64 357 719 3507
Fragments arranged by size:
3507 829 719 429 357 64
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 2032 2329 2844 2940 3143 4549
Size: 110 213 9 297 515 96 203 1406
Cuts at: 4549 4736 1700
Size: 187 2869
Fragments arranged by size:
2869 1406 515 297 213 203 187 110
96 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 2606 3965 4977 2606
Size: 1359 1012 3534
Fragments arranged by size:
3534 1359 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2368 794
Size: 1574 4331
BstBI TT'CG_AA
Cuts at: 2220 4709 2220
Size: 2489 3416
BstEII G'GTnAC_C
Cuts at: 1184 2260 1184
Size: 1076 4829
BstXI CCAn_nnnn'nTGG
Cuts at: 2827 2827
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2226 2508 3235 3602 3987 4004 5456 5542
Size: 254 282 727 367 385 17 1452 86
Cuts at: 5542 5554 5900 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 727 385 367 346 282 254
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2670 2850 2949 3201 513
Size: 552 1605 180 99 252 3217
Fragments arranged by size:
3217 1605 552 252 180 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2041 2512 487
Size: 683 56 815 471 3880
Fragments arranged by size:
3880 815 683 471 56
BtsI GCAGTG_nn'
Cuts at: 3421 4215 4235 3421
Size: 794 20 5091
Fragments arranged by size:
5091 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2088 2123 529
Size: 35 1524 35 4311
Fragments arranged by size:
4311 1524 35 35
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2298 2331 2653
Size: 54 1425 50 275 18 468 33 322
Cuts at: 2653 2673 2784 2842 2894 2952 3352 4241 5870
Size: 20 111 58 52 58 400 889 1629
Cuts at: 5870 8
Size: 43
Fragments arranged by size:
1629 1425 889 468 400 322 275 111
58 58 54 52 50 43 33 20
18
EagI C'GGCC_G
Cuts at: 1537 1812 2894 3352 1537
Size: 275 1082 458 4090
Fragments arranged by size:
4090 1082 458 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3835 1353
Size: 2482 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3092 4492 4866 5012 387
Size: 1064 138 1503 1400 374 146 1280
Fragments arranged by size:
1503 1400 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2776 2849 3104 3968 4565 5352
Size: 393 515 629 73 255 864 597 787
Cuts at: 5352 5620 1239
Size: 268 1524
Fragments arranged by size:
1524 864 787 629 597 515 393 268
255 73
EcoNI CCTnn'n_nnAGG
Cuts at: 2471 2471
Size: 5905
EcoO109I rG'GnC_Cy
Cuts at: 2065 2365 2409 2901 2065
Size: 300 44 492 5069
Fragments arranged by size:
5069 492 300 44
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FseI GG_CCGG'CC
Cuts at: 2848 2848
Size: 5905
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2039 2091 2914 5052 1678
Size: 361 52 823 2138 2531
Fragments arranged by size:
2531 2138 823 361 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 2447 2479 2610
Size: 32 135 32 482 32 361 32 131
Cuts at: 2610 2642 4546 4578 4620 4652 1373
Size: 32 1904 32 42 32 2626
Fragments arranged by size:
2626 1904 482 361 135 131 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2857 3249 3495 669
Size: 988 281 919 392 246 3079
Fragments arranged by size:
3079 988 919 392 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KasI G'GCGC_C
Cuts at: 2087 2910 2087
Size: 823 5082
KpnI G_GTAC'C
Cuts at: 2800 3230 2800
Size: 430 5475
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2622 2727 2770 5019 5203 1305
Size: 1317 105 43 2249 184 2007
Fragments arranged by size:
2249 2007 1317 184 105 43
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2300 2675 2954 10
Size: 54 1425 343 468 375 279 2961
Fragments arranged by size:
2961 1425 468 375 343 279 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2441 2583 2606 3200 3863 4222 4381
Size: 648 1275 142 23 594 663 359 159
Cuts at: 4381 518
Size: 2042
Fragments arranged by size:
2042 1275 663 648 594 359 159 142
23
MspA1I CmG'CkG
Cuts at: 1606 2922 3068 3321 3998 5146 5391 1606
Size: 1316 146 253 677 1148 245 2120
Fragments arranged by size:
2120 1316 1148 677 253 245 146
NaeI GCC'GGC
Cuts at: 2034 2331 2846 2942 3145 2034
Size: 297 515 96 203 4794
Fragments arranged by size:
4794 515 297 203 96
NarI GG'CG_CC
Cuts at: 2088 2911 2088
Size: 823 5082
NcoI C'CATG_G
Cuts at: 513 1065 2670 2949 513
Size: 552 1605 279 3469
Fragments arranged by size:
3469 1605 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NgoMIV G'CCGG_C
Cuts at: 2032 2329 2844 2940 3143 2032
Size: 297 515 96 203 4794
Fragments arranged by size:
4794 515 297 203 96
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 3263 4808 1132
Size: 97 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 2967 3030 2967
Size: 63 5842
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2365 2365
Size: 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2065 2065
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2118 2364 3257 830
Size: 1288 246 893 3478
Fragments arranged by size:
3478 1288 893 246
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
RsrII CG'GwC_CG
Cuts at: 3117 3117
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 2855 3247 1936
Size: 919 392 4594
Fragments arranged by size:
4594 919 392
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SexAI A'CCwGG_T
Cuts at: 2103 2472 2103
Size: 369 5536
SfcI C'TryA_G
Cuts at: 826 1045 2114 2360 2416 3253 4388 5069 5260
Size: 219 1069 246 56 837 1135 681 191
Cuts at: 5260 826
Size: 1471
Fragments arranged by size:
1471 1135 1069 837 681 246 219 191
56
SfoI GGC'GCC
Cuts at: 2089 2912 2089
Size: 823 5082
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 2908 3232 1872
Size: 1036 324 4545
Fragments arranged by size:
4545 1036 324
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2318 4009 4910 5172 820
Size: 197 926 375 1691 901 262 1553
Fragments arranged by size:
1691 1553 926 901 375 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2190 2670 2949 3344 513
Size: 552 1125 480 279 395 3074
Fragments arranged by size:
3074 1125 552 480 395 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2195 3202 3932 3949 4102 4287 4732
Size: 1255 615 1007 730 17 153 185 445
Cuts at: 4732 325
Size: 1498
Fragments arranged by size:
1498 1255 1007 730 615 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4151 5863 96
Size: 274 80 33 51 1443 2174 1712 138
Fragments arranged by size:
2174 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 2615 3241 2615
Size: 626 5279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2683 2683
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 2906 3230 1870
Size: 1036 324 4545
Fragments arranged by size:
4545 1036 324
XmnI GAAnn'nnTTC
Cuts at: 2172 4034 2172
Size: 1862 4043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI RsrII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII
SacI SacII SanDI SapI SfiI SrfI SwaI